Shear stress, temperature, and inoculation concentration influence the adhesion of water-stressed Helicobacter pylori to stainless steel 304 and polypropylene.

Abstract:

:Although molecular techniques have identified Helicobacter pylori in drinking water-associated biofilms, there is a lack of studies reporting what factors affect the attachment of the bacterium to plumbing materials. Therefore, the adhesion of H. pylori suspended in distilled water to stainless steel 304 (SS304) coupons placed on tissue culture plates subjected to different environmental conditions was monitored. The extent of adhesion was evaluated for different water exposure times, using epifluorescence microscopy to count total cell numbers. High shear stresses-estimated through computational fluid dynamics-negatively influenced the adhesion of H. pylori to the substrata (P < 0.001), a result that was confirmed in similar experiments with polypropylene (P < 0.05). However, the temperature and inoculation concentration appeared to have no effect on adhesion (P > 0.05). After 2 hours, H. pylori cells appeared to be isolated on the surface of SS304 and were able to form small aggregates with longer exposure times. However, the formation of a three-dimensional structure was only very rarely observed. This study suggests that the detection of the pathogen in well water described by other authors can be related to the increased ability of H. pylori to integrate into biofilms under conditions of low shear stress. It will also allow a more rational selection of locations to perform molecular or plate culture analysis for the detection of H. pylori in drinking water-associated biofilms.

journal_name

Appl Environ Microbiol

authors

Azevedo NF,Pinto AR,Reis NM,Vieira MJ,Keevil CW

doi

10.1128/AEM.72.4.2936-2941.2006

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

2936-41

issue

4

eissn

0099-2240

issn

1098-5336

pii

72/4/2936

journal_volume

72

pub_type

杂志文章
  • Induction of a futile Embden-Meyerhof-Parnas pathway in Deinococcus radiodurans by Mn: possible role of the pentose phosphate pathway in cell survival.

    abstract::Statistical models were used to predict the effects of tryptone, glucose, yeast extract (TGY) and Mn on biomass formation of the highly radioresistant bacterium Deinococcus radiodurans. Results suggested that glucose had marginal effect on biomass buildup, but Mn was a significant factor for biomass formation. Mn also...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.1.105-112.2000

    authors: Zhang YM,Wong TY,Chen LY,Lin CS,Liu JK

    更新日期:2000-01-01 00:00:00

  • Gut Microbial Divergence between Two Populations of the Hadal Amphipod Hirondellea gigas.

    abstract::Hadal environments sustain diverse microorganisms. A few studies have investigated hadal microbial communities consisting of free-living or particle-associated bacteria and archaea. However, animal-associated microbial communities in hadal environments remain largely unexplored, and comparative analyses of animal gut ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02032-18

    authors: Zhang W,Watanabe HK,Ding W,Lan Y,Tian RM,Sun J,Chen C,Cai L,Li Y,Oguri K,Toyofuku T,Kitazato H,Drazen JC,Bartlett D,Qian PY

    更新日期:2018-12-13 00:00:00

  • Diversity and structure of bacterial communities in Arctic versus Antarctic pack ice.

    abstract::A comprehensive assessment of bacterial diversity and community composition in arctic and antarctic pack ice was conducted through cultivation and cultivation-independent molecular techniques. We sequenced 16S rRNA genes from 115 and 87 pure cultures of bacteria isolated from arctic and antarctic pack ice, respectivel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.11.6610-6619.2003

    authors: Brinkmeyer R,Knittel K,Jürgens J,Weyland H,Amann R,Helmke E

    更新日期:2003-11-01 00:00:00

  • Spatial variability in biodegradation rates as evidenced by methane production from an aquifer.

    abstract::Accurate predictions of carbon and energy cycling rates in the environment depend on sampling frequencies and on the spatial variability associated with biological activities. We examined the variability associated with anaerobic biodegradation rates at two sites in an alluvial sand aquifer polluted by municipal landf...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.10.3632-3639.1994

    authors: Adrian NR,Robinson JA,Suflita JM

    更新日期:1994-10-01 00:00:00

  • Hydrogen production by geobacter species and a mixed consortium in a microbial electrolysis cell.

    abstract::A hydrogen utilizing exoelectrogenic bacterium (Geobacter sulfurreducens) was compared to both a nonhydrogen oxidizer (Geobacter metallireducens) and a mixed consortium in order to compare the hydrogen production rates and hydrogen recoveries of pure and mixed cultures in microbial electrolysis cells (MECs). At an app...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01760-09

    authors: Call DF,Wagner RC,Logan BE

    更新日期:2009-12-01 00:00:00

  • A Novel Glaesserella sp. Isolated from Pigs with Severe Respiratory Infections Has a Mosaic Genome with Virulence Factors Putatively Acquired by Horizontal Transfer.

    abstract::An unknown member of the family Pasteurellaceae was repeatedly isolated from 20- to 24-week-old pigs with severe pulmonary lesions reared on the same farm in Victoria, Australia. The etiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylog...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00092-18

    authors: Watt AE,Browning GF,Legione AR,Bushell RN,Stent A,Cutler RS,Young ND,Marenda MS

    更新日期:2018-05-17 00:00:00

  • Microbial composition and structure of aerobic granular sewage biofilms.

    abstract::Aerobic activated sludge granules are dense, spherical biofilms which can strongly improve purification efficiency and sludge settling in wastewater treatment processes. In this study, the structure and development of different granule types were analyzed. Biofilm samples originated from lab-scale sequencing batch rea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01002-07

    authors: Weber SD,Ludwig W,Schleifer KH,Fried J

    更新日期:2007-10-01 00:00:00

  • Metatranscriptome analysis of the human fecal microbiota reveals subject-specific expression profiles, with genes encoding proteins involved in carbohydrate metabolism being dominantly expressed.

    abstract::The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00502-10

    authors: Booijink CC,Boekhorst J,Zoetendal EG,Smidt H,Kleerebezem M,de Vos WM

    更新日期:2010-08-01 00:00:00

  • Antimicrobial Peptide GH12 Prevents Dental Caries by Regulating Dental Plaque Microbiota.

    abstract::Due to the complex microecology and microenvironment of dental plaque, novel caries prevention strategies require modulating the microbial communities ecologically and reducing the cariogenic properties effectively. Antimicrobial peptide GH12 reduced the lactic acid production and exopolysaccharide (EPS) synthesis of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00527-20

    authors: Jiang W,Wang Y,Luo J,Chen X,Zeng Y,Li X,Feng Z,Zhang L

    更新日期:2020-07-02 00:00:00

  • Multiple Evolutionary Trajectories Have Led to the Emergence of Races in Fusarium oxysporum f. sp. lycopersici.

    abstract::Race 1 isolates of Fusarium oxysporum f. sp. lycopersici (FOL) are characterized by the presence of AVR1 in their genomes. The product of this gene, Avr1, triggers resistance in tomato cultivars carrying resistance gene I In FOL race 2 and race 3 isolates, AVR1 is absent, and hence they are virulent on tomato cultivar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02548-16

    authors: Biju VC,Fokkens L,Houterman PM,Rep M,Cornelissen BJC

    更新日期:2017-02-01 00:00:00

  • Directed construction and analysis of a Sinorhizobium meliloti pSymA deletion mutant library.

    abstract::Resources from the Sinorhizobium meliloti Rm1021 open reading frame (ORF) plasmid libraries were used in a medium-throughput method to construct a set of 50 overlapping deletion mutants covering all of the Rm1021 pSymA megaplasmid except the replicon region. Each resulting pSymA derivative carried a defined deletion o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02974-12

    authors: Yurgel SN,Mortimer MW,Rice JT,Humann JL,Kahn ML

    更新日期:2013-03-01 00:00:00

  • The N-Acetylglucosaminidase LytB of Streptococcus pneumoniae Is Involved in the Structure and Formation of Biofilms.

    abstract::The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00280-20

    authors: Domenech M,García E

    更新日期:2020-05-05 00:00:00

  • Variation in microbial activity in histosols and its relationship to soil moisture.

    abstract::Microbial biomass, dehydrogenase activity, carbon metabolism, and aerobic bacterial populations were examined in cropped and fallow Pahokee muck (a lithic medisaprist) of the Florida Everglades. Dehydrogenase activity was two- to sevenfold greater in soil cropped to St. Augustinegrass (Stenotaphrum secundatum (Walt) K...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.2.313-317.1980

    authors: Tate RL,Terry RE

    更新日期:1980-08-01 00:00:00

  • Virus movement in soil columns flooded with secondary sewage effluent.

    abstract::Secondary sewage effluent containing about 3 X 10(4) plaque-forming units of polio virus type 1 (LSc) per ml was passed through columns 250 cm in length packed with calcareous sand from an area in the Salt River bed used for ground-water recharge of secondary sewage effluent. Viruses were not detected in 1-ml samples ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.4.520-526.1976

    authors: Lance JC,Gerba CP,Melnick JL

    更新日期:1976-10-01 00:00:00

  • Proteolytic activity of the ruminal bacterium Butyrivibrio fibrisolvens.

    abstract::The proteolytic activity of Butyrivibrio fibrisolvens, a ubiquitously distributed bacterial species in the gastrointestinal tracts of ruminants and other mammals, was characterized. The relative proteolytic activity (micrograms of azocasein degraded per hour per milligram of protein) varied greatly with the strain: 0 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.1.51-58.1986

    authors: Cotta MA,Hespell RB

    更新日期:1986-07-01 00:00:00

  • Intergeneric conjugation in Streptomyces peucetius and Streptomyces sp. strain C5: chromosomal integration and expression of recombinant plasmids carrying the chiC gene.

    abstract::Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.1.84-91.2003

    authors: Paranthaman S,Dharmalingam K

    更新日期:2003-01-01 00:00:00

  • Effect of Macrolide and Rifampin Resistance on the Fitness of Rhodococcus equi.

    abstract::Rhodococcus equi is a leading cause of severe pneumonia in foals. Standard treatment is dual antimicrobial therapy with a macrolide and rifampin, but the emergence of macrolide- and rifampin-resistant R. equi isolates is an increasing problem. The objective of this study was to determine the effect of macrolide and/or...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02665-18

    authors: Willingham-Lane JM,Berghaus LJ,Berghaus RD,Hart KA,Giguère S

    更新日期:2019-03-22 00:00:00

  • Molecular cloning, purification, and biochemical characterization of hydantoin racemase from the legume symbiont Sinorhizobium meliloti CECT 4114.

    abstract::Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.1.625-630.2004

    authors: Martínez-Rodríguez S,Las Heras-Vázquez FJ,Mingorance-Cazorla L,Clemente-Jiménez JM,Rodríguez-Vico F

    更新日期:2004-01-01 00:00:00

  • Modular engineering of L-tyrosine production in Escherichia coli.

    abstract::Efficient biosynthesis of L-tyrosine from glucose is necessary to make biological production economically viable. To this end, we designed and constructed a modular biosynthetic pathway for L-tyrosine production in E. coli MG1655 by encoding the enzymes for converting erythrose-4-phosphate (E4P) and phosphoenolpyruvat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06017-11

    authors: Juminaga D,Baidoo EE,Redding-Johanson AM,Batth TS,Burd H,Mukhopadhyay A,Petzold CJ,Keasling JD

    更新日期:2012-01-01 00:00:00

  • Structural and functional differences in two cyclic bacteriocins with the same sequences produced by lactobacilli.

    abstract::Lactobacillus gasseri LA39 and L. reuteri LA6 isolated from feces of the same human infant were found to produce similar cyclic bacteriocins (named gassericin A and reutericin 6, respectively) that cannot be distinguished by molecular weights or primary amino acid sequences. However, reutericin 6 has a narrower spectr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.5.2906-2911.2004

    authors: Kawai Y,Ishii Y,Arakawa K,Uemura K,Saitoh B,Nishimura J,Kitazawa H,Yamazaki Y,Tateno Y,Itoh T,Saito T

    更新日期:2004-05-01 00:00:00

  • A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS3 Family Insertion Sequence, ISBlo11.

    abstract::Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combinati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00824-18

    authors: Sakanaka M,Nakakawaji S,Nakajima S,Fukiya S,Abe A,Saburi W,Mori H,Yokota A

    更新日期:2018-08-17 00:00:00

  • A β-Alanine Catabolism Pathway Containing a Highly Promiscuous ω-Transaminase in the 12-Aminododecanate-Degrading Pseudomonas sp. Strain AAC.

    abstract:UNLABELLED:We previously isolated the transaminase KES23458 from Pseudomonas sp. strain AAC as a promising biocatalyst for the production of 12-aminododecanoic acid, a constituent building block of nylon-12. Here, we report the subsequent characterization of this transaminase. It exhibits activity with a broad substrat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00665-16

    authors: Wilding M,Peat TS,Newman J,Scott C

    更新日期:2016-06-13 00:00:00

  • Differential proteomic analysis of Rickettsia prowazekii propagated in diverse host backgrounds.

    abstract::The obligate intracellular growth of Rickettsia prowazekii places severe restrictions on the analysis of rickettsial gene expression. With a small genome, predicted to code for 835 proteins, identifying which proteins are differentially expressed in rickettsiae that are isolated from different hosts or that vary in vi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05140-11

    authors: Tucker AM,Driskell LO,Pannell LK,Wood DO

    更新日期:2011-07-01 00:00:00

  • Morphological diversity of ruminal bacteriophages from sheep and cattle.

    abstract::Large numbers of bacteriophages (2 x 10(7) to 1 x 10(8)/ml) were present in ruminal fluid from sheep and cattle. Twenty-six distinct types were identified and placed in three morphological groups; several phages possessed unusual structural features. The large numbers and diversity of phages observed indicates a possi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.6.1637-1641.1988

    authors: Klieve AV,Bauchop T

    更新日期:1988-06-01 00:00:00

  • Isolation of new bacterial species from drinking water biofilms and proof of their in situ dominance with highly specific 16S rRNA probes.

    abstract::A polyphasic approach involving cultivation, direct viable counts, rRNA-based phylogenetic classification, and in situ probing was applied for the characterization of the dominant microbial population in a municipal drinking water distribution system. A total of 234 bacterial strains cultivated on R2A medium were scre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.11.4164-4170.1997

    authors: Kalmbach S,Manz W,Szewzyk U

    更新日期:1997-11-01 00:00:00

  • Effect of chlorine dioxide gas on fungi and mycotoxins associated with sick building syndrome.

    abstract::The growth of indoor molds and their resulting products (e.g., spores and mycotoxins) can present health hazards for human beings. The efficacy of chlorine dioxide gas as a fumigation treatment for inactivating sick building syndrome-related fungi and their mycotoxins was evaluated. Filter papers (15 per organism) fea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.9.5399-5403.2005

    authors: Wilson SC,Wu C,Andriychuk LA,Martin JM,Brasel TL,Jumper CA,Straus DC

    更新日期:2005-09-01 00:00:00

  • Metabolism of aflatoxin B1 by rat hepatic microsomes induced by polyhalogenated biphenyl congeners.

    abstract::The metabolism of aflatoxin B1 to aflatoxins M1 and Q1 by rat liver microsomes from animals pretreated with polychlorinated or polybrominated biphenyl congeners depended on the structure of the halogenated biphenyl inducers. Microsomes from rats treated with phenobarbital (PB) or halogenated biphenyls that exhibit PB-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.4.882-886.1985

    authors: Halvorson MR,Phillips TD,Safe SH,Robertson LW

    更新日期:1985-04-01 00:00:00

  • Effect of L-proline on sake brewing and ethanol stress in Saccharomyces cerevisiae.

    abstract::During the fermentation of sake, cells of Saccharomyces cerevisiae are exposed to high concentrations of ethanol, thereby damaging the cell membrane and functional proteins. L-proline protects yeast cells from damage caused by freezing or oxidative stress. In this study, we evaluated the role of intracellular L-prolin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8656-8662.2005

    authors: Takagi H,Takaoka M,Kawaguchi A,Kubo Y

    更新日期:2005-12-01 00:00:00

  • L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches.

    abstract::Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by het...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.3.1869-1873.2004

    authors: van der Kaaij H,Desiere F,Mollet B,Germond JE

    更新日期:2004-03-01 00:00:00

  • Quantitative assessment of factors affecting the recovery of indigenous and released thermophilic bacteria from compost.

    abstract::Thermophilic actinomycetes and bacilli were recovered from mushroom compost by conventional dilution plating and sedimentation chamber-Andersen sampler methods. Excessive growth of thermophilic bacilli on dilution plates accounted for the poor recovery and limited diversity of actinomycete colonies, and this result wa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.12.3107-3112.1988

    authors: Amner W,McCarthy AJ,Edwards C

    更新日期:1988-12-01 00:00:00