Arthrobacter aurescens TC1 atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli.

abstract：:The culturability of Vibrio cholerae O1 serotype Inaba strain 569B was decreased by the addition of glucose to cell suspensions in starvation media. A similar effect was observed with sucrose, maltose, and fructose. We term this inhibitory effect glucose shock. It was not observed with arabinose or xylose or with carb...

journal_title：Applied and environmental microbiology

authors： Shiba T,Hill RT,Straube WL,Colwell RR

更新日期：1995-07-01 00:00:00

abstract：:Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatographically at the rat...

journal_title：Applied and environmental microbiology

authors： David KA,Apte SK,Banerji A,Thomas J

更新日期：1980-05-01 00:00:00

abstract：:The distribution of Clostridium botulinum serotypes A, B, E, and F in Finnish trout farms was examined. A total of 333 samples were tested with a neurotoxin-specific PCR assay. C. botulinum type E was found in 68% of the farm sediment samples, in 15% of the fish intestinal samples, and in 5% of the fish skin samples. ...

journal_title：Applied and environmental microbiology

authors： Hielm S,Björkroth J,Hyytiä E,Korkeala H

更新日期：1998-11-01 00:00:00

abstract：:We investigated long-chain fatty acid (LCFA)-degrading anaerobic microbes by enrichment, isolation, and RNA-based stable isotope probing (SIP). Primary enrichment cultures were made with each of four LCFA substrates (palmitate, stearate, oleate, or linoleate, as the sole energy source) at 55 degrees C or 37 degrees C ...

journal_title：Applied and environmental microbiology

authors： Hatamoto M,Imachi H,Ohashi A,Harada H

更新日期：2007-02-01 00:00:00

abstract：:The transcription factors sinR and abrB are involved in the control of sporulation initiation in Bacillus subtilis. We identified a single homologue to sinR and three highly similar homologues to abrB, designated abrB310, abrB1941, and abrB3647, in Clostridium acetobutylicum ATCC 824. Using reporter vectors, we showed...

journal_title：Applied and environmental microbiology

authors： Scotcher MC,Rudolph FB,Bennett GN

更新日期：2005-04-01 00:00:00

abstract：:Three of 36 raw milk isolates of Yersinia enterocolitica produced enterotoxin in milk at 25 degrees C, but not at 4 degrees C. No strain tested could survive pasteurization. ...

journal_title：Applied and environmental microbiology

authors： Francis DW,Spaulding PL,Lovett J

更新日期：1980-07-01 00:00:00

abstract：:Nitrate was formed from ammonium at pH 3.2 to 6.1 in suspensions of a naturally acid forest soil; the maximum rates of formation occurred at ca. pH 4 to 5. Nitrate was also formed from soil nitrogen in suspensions incubated at 50 degrees C. Autotrophic nitrifying bacteria could not be isolated from this soil. Enrichme...

journal_title：Applied and environmental microbiology

authors： Stroo HF,Klein TM,Alexander M

更新日期：1986-11-01 00:00:00

abstract：:A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands co...

journal_title：Applied and environmental microbiology

authors： Royer JC,Nakas JP

更新日期：1990-06-01 00:00:00

abstract：:The degradation of three polycyclic aromatic hydrocarbons (PAH), pyrene (PYR), benz[a]anthracene (BAA), and benzo[a]pyrene (BaP), by Mycobacterium sp. strain RJGII-135 was studied. The bacterium was isolated from an abandoned coal gasification site soil by analog enrichment techniques and found to mineralize [14C]PYR....

journal_title：Applied and environmental microbiology

authors： Schneider J,Grosser R,Jayasimhulu K,Xue W,Warshawsky D

更新日期：1996-01-01 00:00:00

abstract：:Differing incidences of Vibrio parahaemolyticus reported by separate government agencies are attributable to sample handling and subsequent isolation techniques. ...

journal_title：Applied and environmental microbiology

authors： Clark AG

更新日期：1977-11-01 00:00:00

abstract：:The study site located in the Mediterranean Sea was visited eight times in 2005 and 2006 to collect samples from the epipelagic (5 m), mesopelagic (200 m, 600 m), and bathypelagic (1,000 m, 2,000 m) zones. Randomly amplified polymorphic DNA PCR (RAPD-PCR) analysis was used to obtain fingerprints from microbial and vir...

journal_title：Applied and environmental microbiology

authors： Winter C,Weinbauer MG

更新日期：2010-10-01 00:00:00

abstract：:A field evaluation of double violet agar for the isolation and presumptive identification of Klebsiella pneumoniae from water has been performed. Water from the North Oconee River, Clarke County, Ga., was cultured for presence of klebsiellae using the membrane filter technique. Colonies were presumptively identified a...

journal_title：Applied and environmental microbiology

authors： Campbell LM,Roth IL,Klein RD

更新日期：1976-02-01 00:00:00

abstract：:The probability of recovering pathogenic Escherichia coli from food by the Bacteriological Analytical Manual method was determined by the effects of several factors: the number of strains per food, the ability of pathogenic strains to survive enrichment, and the frequency of plasmid loss during enrichment. Biochemical...

journal_title：Applied and environmental microbiology

authors： Hill WE,Ferreira JL,Payne WL,Jones VM

更新日期：1985-06-01 00:00:00

abstract：:An in-depth study of the continuous cell line designated BGM is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. Based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, ...

journal_title：Applied and environmental microbiology

authors： Dahling DR,Wright BA

更新日期：1986-04-01 00:00:00

abstract：:Washed and air-oxidized proteins from Sporomusa ovata cleaved the C-O bond of methanol or methoxyaromatics and transferred the methyl to dl-tetrahydrofolate. The reactions strictly required a reductive activation by titanium citrate, catalytic amounts of ATP, and the addition of dl-tetrahydrofolate. Methylcorrinoid-co...

journal_title：Applied and environmental microbiology

authors： Stupperich E,Konle R

更新日期：1993-09-01 00:00:00

abstract：:The ability of staphylococcal strains isolated from different anatomical sites in 133 healthy goats to produce toxic shock syndrome toxin 1 (TSST-1) and the presence of antibodies to this toxin in serum and milk were studied. The enzyme-linked immunosorbent assay method was used to detect both the toxin and the presen...

journal_title：Applied and environmental microbiology

authors： Valle J,Vadillo S,Piriz S,Gomez-Lucia E

更新日期：1991-03-01 00:00:00

abstract：:Hot-water extracts of mesquite (Prosopis glandulosa) wood were assayed for their total carbohydrate, reducing sugar, and glucose content. These hydrolysates were then used as complete media for yeast growth. A total of 10 strains of yeasts were evaluated for their biomass production in the mesquite wood hydrolysates. ...

journal_title：Applied and environmental microbiology

authors： Stanlake GJ

更新日期：1986-08-01 00:00:00

abstract：:The aim of this work was to investigate the medium requirements for growth and production of exopolysaccharides by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772. The strain was grown in batch cultures on a chemically defined medium, and the technique of single omission of medium components was applied to deter...

journal_title：Applied and environmental microbiology

authors： Grobben GJ,Chin-Joe I,Kitzen VA,Boels IC,Boer F,Sikkema J,Smith MR,de Bont JA

更新日期：1998-04-01 00:00:00

abstract：:1,2-Dichloroethane (1,2-DCA) and 1,2-dibromoethane (ethylene dibromide [EDB]) contaminate groundwater at many hazardous waste sites. The objectives of this study were to measure yields, maximum specific growth rates (μ), and half-saturation coefficients (K(S)) in enrichment cultures that use 1,2-DCA and EDB as termina...

journal_title：Applied and environmental microbiology

authors： Yu R,Peethambaram HS,Falta RW,Verce MF,Henderson JK,Bagwell CE,Brigmon RL,Freedman DL

更新日期：2013-02-01 00:00:00

abstract：:Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), ...

journal_title：Applied and environmental microbiology

authors： Unnerstad H,Nilsson I,Ericsson H,Danielsson-Tham ML,Bille J,Bannerman E,Tham W

更新日期：1999-05-01 00:00:00

abstract：:Interactions of soybean with Bradyrhizobium japonicum 123 (serogroup 123) and 138 (serogroup c1) were used to examine the relationship between early infection rates, competition for nodulation, and patterns of nodule occupancy. Both strains formed more infections in autoclaved soil (sterile soil) than in untreated soi...

journal_title：Applied and environmental microbiology

authors： Zdor RE,Pueppke SG

更新日期：1988-08-01 00:00:00

abstract：:Controlling antibiotic-resistant (ART) bacteria in cheese fermentation is important for food safety and public health. A plant-maintained culture was found to be a potential source for ART bacterial contamination in cheese fermentation. Antibiotics had a detectable effect on the ART population from contamination in th...

journal_title：Applied and environmental microbiology

authors： Li X,Li Y,Alvarez V,Harper WJ,Wang HH

更新日期：2011-10-01 00:00:00

abstract：:In 2004, more than 1,230 cases of gastroenteritis due to pandemic O3:K6 strains of Vibrio parahaemolyticus were reported in southern Sinaloa, a state in Northwestern Mexico. Recurrent sporadic cases arose from 2004 to 2010, spreading from the south to the north. In the present study, Vibrio parahaemolyticus was detect...

journal_title：Applied and environmental microbiology

authors： Velazquez-Roman J,León-Sicairos N,Flores-Villaseñor H,Villafaña-Rauda S,Canizalez-Roman A

更新日期：2012-03-01 00:00:00

abstract：:A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in the environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE or DGGE, respectively). The sp...

journal_title：Applied and environmental microbiology

authors： Heuer H,Krsek M,Baker P,Smalla K,Wellington EM

更新日期：1997-08-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized antimicrobial peptides with substantial posttranslational modifications. They are characterized by the unique amino acids lanthionine and methyllanthionine, which are introduced by dehydration of Ser/Thr residues and linkage of the resulting dehydrated amino acids with Cys resi...

journal_title：Applied and environmental microbiology

authors： Basi-Chipalu S,Dischinger J,Josten M,Szekat C,Zweynert A,Sahl HG,Bierbaum G

更新日期：2015-05-15 00:00:00

abstract：:Forty-two Helicobacter isolates were isolated from swine feces in The Netherlands and Denmark. All 12 isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis ( approximately 99% similarity). Species-specific PCR indicated that all of the isolates were H. canadensis isolates. Although the ...

journal_title：Applied and environmental microbiology

authors： Inglis GD,McConville M,de Jong A

更新日期：2006-06-01 00:00:00

abstract：:We have previously reported that the prominent industrial enzyme producer Trichoderma reesei (teleomorph Hypocrea jecorina; Hypocreales, Ascomycota, Dikarya) has a genetically isolated, sympatric sister species devoid of sexual reproduction and which is constituted by the majority of anamorphic strains previously attr...

journal_title：Applied and environmental microbiology

authors： Atanasova L,Jaklitsch WM,Komoń-Zelazowska M,Kubicek CP,Druzhinina IS

更新日期：2010-11-01 00:00:00

abstract：:The influence of nisin on the proton motive force (delta p) generated by glucose-energized cells of the obligate putrefactive anaerobe Clostridium sporogenes PA 3679 was determined. The components of delta p, the transmembrane potential (delta psi) and the pH gradient (delta pH), were determined from the distributions...

journal_title：Applied and environmental microbiology

authors： Okereke A,Montville TJ

更新日期：1992-08-01 00:00:00

abstract：:In this study, a number of Listeria monocytogenes strains of different origins were evaluated for in vitro invasion capacity for various human cell types (monocytic THP-1, enterocytic Caco-2, and hepatocytic HepG2 cells) and for expression levels of specific virulence genes. For THP-1 cells, no differences between cli...

journal_title：Applied and environmental microbiology

authors： Werbrouck H,Grijspeerdt K,Botteldoorn N,Van Pamel E,Rijpens N,Van Damme J,Uyttendaele M,Herman L,Van Coillie E

更新日期：2006-06-01 00:00:00

abstract：:We recently described a new method to activate antibiotic production in bacteria by introducing a mutation conferring resistance to a drug such as streptomycin, rifampin, paromomycin, or gentamicin. This method, however, enhanced antibiotic production by only up to an order of magnitude. Working with Streptomyces coel...

journal_title：Applied and environmental microbiology

authors： Wang G,Hosaka T,Ochi K

更新日期：2008-05-01 00:00:00