Engineering of Bacillus subtilis for enhanced total synthesis of folic acid.

abstract：:There is a wealth of evidence indicating that mobile genetic elements can spread in natural microbial communities. However, little is known regarding the fraction of the community that actually engages in this behavior. Here we report on a new approach to quantify the fraction of a bacterial community that is able to ...

journal_title：Applied and environmental microbiology

authors： Musovic S,Dechesne A,Sørensen J,Smets BF

更新日期：2010-07-01 00:00:00

abstract：:Q fever, caused by Coxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection...

journal_title：Applied and environmental microbiology

authors： de Bruin A,de Groot A,de Heer L,Bok J,Wielinga PR,Hamans M,van Rotterdam BJ,Janse I

更新日期：2011-09-01 00:00:00

abstract：:We isolated thermotolerant Gluconobacter strains that are able to produce 5-keto-d-gluconic acid (5KGA) at 37 degrees C, a temperature at which regular mesophilic 5KGA-producing strains showed much less growth and 5KGA production. The thermotolerant strains produced 2KGA as the major product at both 30 and 37 degrees ...

journal_title：Applied and environmental microbiology

authors： Saichana I,Moonmangmee D,Adachi O,Matsushita K,Toyama H

更新日期：2009-07-01 00:00:00

abstract：:Streptomyces exfoliatus SMF19 produced two types of extracellular beta-lactamase-inhibiting proteins, 48 (beta LIP-I) and 33 (beta LIP-II) kDa. The inhibition mode of beta LIP-I on Bacto Penase and benzylpenicillin as substrates was uncompetitive (i.e., both Km and Vmax were changed), while that of beta LIP-II was non...

journal_title：Applied and environmental microbiology

authors： Kim MK,Lee KJ

更新日期：1994-03-01 00:00:00

abstract：:Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precisio...

journal_title：Applied and environmental microbiology

authors： Walker M,Redelman D

更新日期：2004-03-01 00:00:00

abstract：:Three strains of Streptococcus bovis, a homolactic bacterium capable of utilizing starch, were evaluated for growth kinetics and ability to decrease the pH of alfalfa silage. A selected strain was evaluated for its competitiveness as an inoculant with Enterococcus faecium, an organism used in inoculants, and for its a...

journal_title：Applied and environmental microbiology

authors： Jones BA,Muck RE,Ricke SC

更新日期：1991-10-01 00:00:00

abstract：:The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and w...

journal_title：Applied and environmental microbiology

authors： Buttner MP,Cruz P,Stetzenbach LD,Klima-Comba AK,Stevens VL,Cronin TD

更新日期：2004-08-01 00:00:00

abstract：:The influence of substrate and enzyme concentrations on the rate of saccharification of two defined insoluble cellulose substrates, Avicel (FMC Corp., Philadelphia, Pa.) and Solka-Floc (James River Co., Berlin, N.H.), by the cellulase enzyme system of Trichoderma viride was evaluated. In the assays, enzyme concentrati...

journal_title：Applied and environmental microbiology

authors： Liaw ET,Penner MH

更新日期：1990-08-01 00:00:00

abstract：:In this study, we examine bacterial attachment and survival on a titanium (Ti) cathode coated with various carbon nanomaterials (CNM): pristine carbon nanotubes (CNT), oxidized carbon nanotubes (O-CNT), oxidized-annealed carbon nanotubes (OA-CNT), carbon black (CB), and reduced graphene oxide (rGO). The carbon nanomat...

journal_title：Applied and environmental microbiology

authors： Zhang Q,Nghiem J,Silverberg GJ,Vecitis CD

更新日期：2015-07-01 00:00:00

abstract：:Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far. CDT-V is frequently associated with Shiga-toxigenic E. coli (STEC), enterohemorrhagic E. coli (EHEC) O157 strains, and str...

journal_title：Applied and environmental microbiology

authors： Sváb D,Horváth B,Maróti G,Dobrindt U,Tóth I

更新日期：2013-08-01 00:00:00

abstract：:We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of th...

journal_title：Applied and environmental microbiology

authors： Qian C,Wong CC,Swarup S,Chiam KH

更新日期：2013-08-01 00:00:00

abstract：:High-performance liquid chromatography with a cation-exchange resin-packed column was used to determine fermentation products of several known and unknown Clostridium species. The column was operated at 30 degrees C, and isocratic elution was done with 0.013 N H(2)SO(4). Sample preparation for high-performance liquid ...

journal_title：Applied and environmental microbiology

authors： Ehrlich GG,Goerlitz DF,Bourell JH,Eisen GV,Godsy EM

更新日期：1981-11-01 00:00:00

abstract：:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

journal_title：Applied and environmental microbiology

authors： Memmott SD,Ha YS,Dickman MB

更新日期：2002-04-01 00:00:00

abstract：:A novel enterotoxin gene was cloned from Bacillus cereus FM1, and its nucleotide sequence was determined. Previously, a 45-kDa protein causing characteristic enterotoxin symptoms in higher animals had been isolated (K. Shinagawa, p. 181-193, in A. E. Pohland et al., ed., Microbial Toxins in Foods and Feeds, 1990) from...

journal_title：Applied and environmental microbiology

authors： Asano SI,Nukumizu Y,Bando H,Iizuka T,Yamamoto T

更新日期：1997-03-01 00:00:00

abstract：:Nitrate, sulfate, and carbonate were used as electron acceptors to examine the anaerobic biodegradability of chlorinated aromatic compounds in estuarine and freshwater sediments. The respective denitrifying, sulfidogenic, and methanogenic enrichment cultures were established on each of the monochlorinated phenol and m...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Rivera MD,Young LY

更新日期：1993-04-01 00:00:00

abstract：:High concentrations of free metal ions in the environment can be detrimental to bacterial survival. However, bacteria utilize strategies, including the activation of stress response pathways and immobilizing chemical elements on their surface, to limit this toxicity. In this study, we characterized LA4131, the HtpX-li...

journal_title：Applied and environmental microbiology

authors： Henry R,Lo M,Khoo C,Zhang H,Boysen RI,Picardeau M,Murray GL,Bulach DM,Adler B

更新日期：2013-08-01 00:00:00

abstract：:The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was no...

journal_title：Applied and environmental microbiology

authors： Elsen A,Declerck S,De Waele D

更新日期：2003-07-01 00:00:00

abstract：:Of 98 suspected food-borne Clostridium perfringens isolates obtained from a nationwide survey by the Food and Consumer Product Safety Authority in The Netherlands, 59 strains were identified as C. perfringens type A. Using PCR-based techniques, the cpe gene encoding enterotoxin was detected in eight isolates, showing ...

journal_title：Applied and environmental microbiology

authors： Xiao Y,Wagendorp A,Moezelaar R,Abee T,Wells-Bennik MH

更新日期：2012-10-01 00:00:00

abstract：:Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of eac...

journal_title：Applied and environmental microbiology

authors： Ishihara K,Shimokubo N,Sakagami A,Ueno H,Muramatsu Y,Kadosawa T,Yanagisawa C,Hanaki H,Nakajima C,Suzuki Y,Tamura Y

更新日期：2010-08-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) viruses in some fungi are associated with hypovirulence and have been used or proposed as biological control agents. We isolated 7.5-kb dsRNAs from 13 of 286 field strains of Fusarium graminearum isolated from maize in Korea. One of these strains, DK21, was examined in more detail. This str...

journal_title：Applied and environmental microbiology

authors： Chu YM,Jeon JJ,Yea SJ,Kim YH,Yun SH,Lee YW,Kim KH

更新日期：2002-05-01 00:00:00

abstract：:Cryptosporidium parvum is an apicomplexan parasite that infects humans and ruminants. C. parvum isolated from cattle in northeastern Turkey and in Israel was genotyped using multiple polymorphic genetic markers, and the two populations were compared to assess the effect of cattle husbandry on the parasite's population...

journal_title：Applied and environmental microbiology

authors： Tanriverdi S,Markovics A,Arslan MO,Itik A,Shkap V,Widmer G

更新日期：2006-04-01 00:00:00

abstract：:Five autoagglutinating Aeromonas hydrophila isolates recovered from eels and humans were assigned to serogroups O:14 and O:81 of the Sakazaki and Shimada (National Institutes of Health) scheme. They had the following properties in common: positive precipitation after boiling, moderate surface hydrophobicity (salt-aggr...

journal_title：Applied and environmental microbiology

authors： Esteve C,Alcaide E,Canals R,Merino S,Blasco D,Figueras MJ,Tomás JM

更新日期：2004-10-01 00:00:00

abstract：:Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat lab...

journal_title：Applied and environmental microbiology

authors： Ishibashi Y,Cervantes C,Silver S

更新日期：1990-07-01 00:00:00

abstract：:Pyocins are bacteriocins secreted by Pseudomonas aeruginosa, and they assist in the colonization of different niches. A major subset of these antibacterial proteins adopt a modular organization characteristic of polymorphic toxins. They include a receptor-binding domain, a segment enabling membrane passage, and a toxi...

journal_title：Applied and environmental microbiology

authors： Ghequire MGK,Öztürk B

更新日期：2018-08-31 00:00:00

abstract：:The expanded Fermi solution was originally developed for estimating the number of food-poisoning victims when information concerning the circumstances of exposure is scarce. The method has been modified for estimating the initial number of pathogenic or probiotic cells or spores so that enough of them will survive the...

journal_title：Applied and environmental microbiology

authors： Peleg M,Normand MD,Horowitz J,Corradini MG

更新日期：2011-01-01 00:00:00

abstract：:Copper resistance mechanisms provide an important adaptive advantage to plant pathogenic bacteria under exposure to copper treatments. Copper resistance determinants have been described in Pseudomonas syringae pv. syringae (Pss) strains isolated from mango intimately associated with 62 kb plasmids belonging to the pPT...

journal_title：Applied and environmental microbiology

authors： Aprile F,Heredia-Ponce Z,Cazorla FM,de Vicente A,Gutiérrez-Barranquero JA

更新日期：2020-12-23 00:00:00

abstract：:Growth of the ruminal bacteria Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1, and R. albus 7 followed Monod kinetics with respect to concentrations of individual pure cellodextrins (cellobiose, cellotriose, cellotetraose, cellopentaose, and cellohexaose). Under the conditions tested, R. flavefaciens FD-...

journal_title：Applied and environmental microbiology

authors： Shi Y,Weimer PJ

更新日期：1996-03-01 00:00:00

abstract：:The fall armyworm, Spodoptera frugiperda, is an invasive maize pest that has spread from the Americas into Africa and Asia and causes severe crop damage worldwide. Most populations of S. frugiperda show low susceptibility to Bacillus thuringiensis (Bt) Cry1Ab or Cry1Ac toxins, which have been proved to be effective ag...

journal_title：Applied and environmental microbiology

authors： Gómez I,Ocelotl J,Sánchez J,Aguilar-Medel S,Peña-Chora G,Lina-Garcia L,Bravo A,Soberón M

更新日期：2020-10-28 00:00:00

abstract：:During batch fermentation, the rate of ethanol production per milligram of cell protein is maximal for a brief period early in this process and declines progressively as ethanol accumulates in the surrounding broth. Our studies demonstrate that the removal of this accumulated ethanol does not immediately restore ferme...

journal_title：Applied and environmental microbiology

authors： Dombek KM,Ingram LO

更新日期：1987-06-01 00:00:00

abstract：:Arbitrarily primed polymerase chain reaction, with incorporation of either radioactive or fluorescent labels, was used as a rapid and sensitive method for obtaining genomic fingerprints of strains of Lactococcus lactis. Closely related strains produced almost identical fingerprints. Fingerprints of other strains showe...

journal_title：Applied and environmental microbiology

authors： Cancilla MR,Powell IB,Hillier AJ,Davidson BE

更新日期：1992-05-01 00:00:00