Amplification of DNA polymerase gene fragments from viruses infecting microalgae.

abstract：:Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of s...

journal_title：Applied and environmental microbiology

authors： Saikaly PE,Barlaz MA,de Los Reyes FL 3rd

更新日期：2007-10-01 00:00:00

abstract：:The bphC and bphD genes of Pseudomonas putida involved in the catabolism of polychlorinated biphenyls or biphenyl were identified, localized, and studied for expression in Escherichia coli. This was achieved by cloning a 2.4-kilobase (kb) DNA fragment of recombinant cosmid pOH101 into HindIII site of pUC plasmids down...

journal_title：Applied and environmental microbiology

authors： Khan AA,Walia SK

更新日期：1990-04-01 00:00:00

abstract：:White rot basidiomycetes were able to biodegrade styrene (1-phenylethene) graft copolymers of lignin containing different proportions of lignin and polystyrene [poly(1-phenylethylene)]. The biodegradation tests were run on lignin-styrene copolymerization products which contained 10.3, 32.2, and 50.4% (wt/wt) lignin. T...

journal_title：Applied and environmental microbiology

authors： Milstein O,Gersonde R,Huttermann A,Chen MJ,Meister JJ

更新日期：1992-10-01 00:00:00

abstract：:One fluorescent compound previously named TDP-2 was isolated and purified from a rice culture of Fusarium equiseti (Alaska 2-2). Mass spectral and nuclear magnetic resonance data indicated that it is a C-3'-N-acetyl derivative of fusarochromanone, a newly discovered mycotoxin. Time course studies of synthesis of these...

journal_title：Applied and environmental microbiology

authors： Xie WP,Mirocha CJ,Pawlosky RJ,Wen YC,Xu XG

更新日期：1989-04-01 00:00:00

abstract：:Staphylococcus aureus Cowan 1 cells were armed with anti-flagellar (anti-H) antibody produced in rabbits immunized with flagellar core protein prepared from Vibrio vulnificus. This reagent was assessed by coagglutination for its capacity to agglutinate and identify V. vulnificus. A species-specific H antigen is expres...

journal_title：Applied and environmental microbiology

authors： Simonson J,Siebeling RJ

更新日期：1986-12-01 00:00:00

abstract：:Lantibiotics are antimicrobial peptides belonging to the family of ribosomally synthesized and posttranslationally modified peptides (RiPPs) and feature thioether linkages in their structures. In this study, we identified the biosynthetic gene cluster of a cinnamycin analog, named mathermycin, from Marinactinospora th...

journal_title：Applied and environmental microbiology

authors： Chen E,Chen Q,Chen S,Xu B,Ju J,Wang H

更新日期：2017-07-17 00:00:00

abstract：:Selective growth inhibitors of Sphaerotilus natans were detected in the culture broths of several strains of Streptomyces. Anslimins A and B were isolated from the culture broth of Streptomyces oganonensis by adsorption on resinous adsorbent followed by elution with 50% aqueous acetone. The A and B components were the...

journal_title：Applied and environmental microbiology

authors： Takiguchi Y,Yoshikawa H,Terao M

更新日期：1980-06-01 00:00:00

abstract：:The existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as Thiobacillus ferroox...

journal_title：Applied and environmental microbiology

authors： Sugio T,White KJ,Shute E,Choate D,Blake RC

更新日期：1992-01-01 00:00:00

abstract：:We investigated the prevalence of sapoviruses (SaVs) in the Tamagawa River in Japan from April 2003 to March 2004 and performed genetic analysis of the SaV genes identified in river water. A total of 60 river water samples were collected from five sites along the river, and 500 ml was concentrated using the cation-coa...

journal_title：Applied and environmental microbiology

authors： Kitajima M,Oka T,Haramoto E,Katayama H,Takeda N,Katayama K,Ohgaki S

更新日期：2010-04-01 00:00:00

abstract：:Microbial biomass, dehydrogenase activity, carbon metabolism, and aerobic bacterial populations were examined in cropped and fallow Pahokee muck (a lithic medisaprist) of the Florida Everglades. Dehydrogenase activity was two- to sevenfold greater in soil cropped to St. Augustinegrass (Stenotaphrum secundatum (Walt) K...

journal_title：Applied and environmental microbiology

authors： Tate RL,Terry RE

更新日期：1980-08-01 00:00:00

abstract：:[This corrects the article on p. 2161 in vol. 59.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1993-12-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:A repeated cross-sectional study was conducted to identify farm management, environment, weather, and landscape factors that predict the count of generic Escherichia coli on spinach at the preharvest level. E. coli was enumerated for 955 spinach samples collected on 12 farms in Texas and Colorado between 2010 and 2012...

journal_title：Applied and environmental microbiology

authors： Park S,Navratil S,Gregory A,Bauer A,Srinath I,Szonyi B,Nightingale K,Anciso J,Jun M,Han D,Lawhon S,Ivanek R

更新日期：2015-04-01 00:00:00

abstract：:In Comamonas sp. strain JS46, 3-nitrobenzoate (3Nba) is initially oxidized at the 3,4 position by a dioxygenase, which results in release of nitrite and production of protocatechuate. The locus coding for the 3Nba dioxygenase (designated mnb, for m-nitrobenzoate) was mobilized from strain JS46 using a plasmid capture ...

journal_title：Applied and environmental microbiology

authors： Providenti MA,Shaye RE,Lynes KD,McKenna NT,O'brien JM,Rosolen S,Wyndham RC,Lambert IB

更新日期：2006-04-01 00:00:00

abstract：:Saccharomyces pastorianus strains are hybrids of Saccharomyces cerevisiae and Saccharomyces eubayanus that have been domesticated for centuries in lager beer brewing environments. As sequences and structures of S. pastorianus genomes are being resolved, molecular mechanisms and evolutionary origins of several industri...

journal_title：Applied and environmental microbiology

authors： Brouwers N,Brickwedde A,Gorter de Vries AR,van den Broek M,Weening SM,van den Eijnden L,Diderich JA,Bai FY,Pronk JT,Daran JG

更新日期：2019-10-30 00:00:00

abstract：:Legionella pneumophila was detected and identified by an immunoblot assay using a monoclonal antibody specific to serogroups 1 to 8. Samples containing L. pneumophila were plated on buffered charcoal yeast extract agar supplemented with glycine, vancomycin, and polymyxin B. After incubation at 35 degrees C for 3 days,...

journal_title：Applied and environmental microbiology

authors： Bérubé A,Trudel M,Payment P

更新日期：1989-06-01 00:00:00

abstract：:Most yeast species can ferment sugars to ethanol, but only a few can grow in the complete absence of oxygen. Oxygen availability might, therefore, be a key parameter in spoilage of food caused by fermentative yeasts. In this study, the oxygen requirement and regulation of alcoholic fermentation were studied in batch c...

journal_title：Applied and environmental microbiology

authors： Rodrigues F,Côrte-Real M,Leão C,van Dijken JP,Pronk JT

更新日期：2001-05-01 00:00:00

abstract：:The microporous polyethylene hollow-fiber membrane has a unique microfibrile structure throughout its depth and has been found to possess the functions of filtration and adsorption of endotoxin in water. The membrane has a maximum pore diameter of approximately 0.04 micron, a diameter which is within the range of micr...

journal_title：Applied and environmental microbiology

authors： Sawada Y,Fujii R,Igami I,Kawai A,Kamiki T,Niwa M

更新日期：1986-04-01 00:00:00

abstract：:Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...

journal_title：Applied and environmental microbiology

authors： Folsom BR,Schieche DR,DiGrazia PM,Werner J,Palmer S

更新日期：1999-11-01 00:00:00

abstract：:Microbial mercury methylation and methylmercury decomposition were examined in Lake Clara, an oligotrophic northern Wisconsin seepage lake, using radioisotopic tracers. Methylation activity was near background in the water column, was greatest in the profundal surficial sediments, and decreased with depth in sediment ...

journal_title：Applied and environmental microbiology

authors： Korthals ET,Winfrey MR

更新日期：1987-10-01 00:00:00

abstract：:Sterol demethylation inhibitor (DMI) fungicides are widely used to control fungi pathogenic to humans and plants. Resistance to DMIs is mediated either through alterations in the structure of the target enzyme CYP51 (encoding 14alpha-demethylase), through increased expression of the CYP51 gene, or through increased ex...

journal_title：Applied and environmental microbiology

authors： Ma Z,Proffer TJ,Jacobs JL,Sundin GW

更新日期：2006-04-01 00:00:00

abstract：:[This corrects the article on p. 1743 in vol. 64, PMID: 9572946.]. ...

journal_title：Applied and environmental microbiology

authors： Kaucner C,Stinear T

更新日期：1998-11-01 00:00:00

abstract：:Seawater used in mariculture has been suspected of being a potential source of infection. In this study, the lethal effects of low-amperage electric treatment on microorganisms were examined in natural seawater and in seawater inoculated with Vibrio parahaemolyticus. In both cases, bacteria including V. parahaemolytic...

journal_title：Applied and environmental microbiology

authors： Park JC,Lee MS,Lee DH,Park BJ,Han DW,Uzawa M,Takatori K

更新日期：2003-04-01 00:00:00

abstract：UNLABELLED:We previously isolated the transaminase KES23458 from Pseudomonas sp. strain AAC as a promising biocatalyst for the production of 12-aminododecanoic acid, a constituent building block of nylon-12. Here, we report the subsequent characterization of this transaminase. It exhibits activity with a broad substrat...

journal_title：Applied and environmental microbiology

authors： Wilding M,Peat TS,Newman J,Scott C

更新日期：2016-06-13 00:00:00

abstract：:The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...

journal_title：Applied and environmental microbiology

authors： Rawlings TK,Ruiz GM,Colwell RR

更新日期：2007-12-01 00:00:00

abstract：:Quorum sensing (QS) regulates Phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that QS is not important for antagonism in algal cultures. QS mutants reduced Vibrio anguillarum to the same extent as the wild type. Consequently, a combination of probiotic Phaeobacter and QS inhibitors ...

journal_title：Applied and environmental microbiology

authors： Prol García MJ,D'Alvise PW,Gram L

更新日期：2013-09-01 00:00:00

abstract：:Salmonella species are pathogenic bacteria often detected in sewage, freshwater, marine coastal water, and groundwater. Salmonella spp. can survive for long periods in natural waters, and the persistence of specific and epidemic strains is of great concern in public health. However, the diversity of species found in t...

journal_title：Applied and environmental microbiology

authors： Baudart J,Lemarchand K,Brisabois A,Lebaron P

更新日期：2000-04-01 00:00:00

abstract：:A beta-mannanase (EC 3.2.1.78) from Vibrio sp. strain MA-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. The final ion-exchange chromatography Mono Q yielded one major active fraction and three minor acti...

journal_title：Applied and environmental microbiology

authors： Tamaru Y,Araki T,Amagoi H,Mori H,Morishita T

更新日期：1995-12-01 00:00:00

abstract：:Microorganisms in diverse terrestrial surface and subsurface environments can anaerobically catalyze the oxidative dissolution of uraninite. While a limited quantity (∼5 to 12 μmol liter(-1)) of uranium is oxidatively dissolved in pure culture studies, the metabolism is coupled to electron transport, providing the pot...

journal_title：Applied and environmental microbiology

authors： Weber KA,Thrash JC,Van Trump JI,Achenbach LA,Coates JD

更新日期：2011-07-01 00:00:00

abstract：:A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Tran B,Sangermano LR,Palmer CJ

更新日期：1994-07-01 00:00:00