Abstract:
:The biological utilization of CO(2) and H(2) for the formation of short-chain fatty acids was studied by using a mixed culture of bacteria. Optimization of a medium was carried out in continuous culture to identify limiting factors which controlled growth and production of organic acids. The optimal pH for growth and acid production was 7.0 at 37 degrees C; the maximal cell concentration obtained was 5.9 g of cells per liter (dry weight), and the maximal amount of volatile acids formed was 4.7 g/liter, with acetic acid as the predominant acid. With the optimized medium, it was found that the rate of transfer of hydrogen or carbon dioxide, or both, from gas to liquid was the limiting factor which controlled growth and production of acids.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Goldberg I,Cooney CLdoi
10.1128/AEM.41.1.148-154.1981keywords:
subject
Has Abstractpub_date
1981-01-01 00:00:00pages
148-54issue
1eissn
0099-2240issn
1098-5336journal_volume
41pub_type
杂志文章abstract::In order to understand the role of the mar locus in Salmonella with regard to multiple antibiotic resistance, cyclohexane resistance, and outer membrane protein F (OmpF) regulation, a marA::gfp reporter mutant was constructed in an antibiotic-sensitive Salmonella enterica serovar Typhimurium DT104 background. Salicyla...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.3.1190-1197.2001
更新日期:2001-03-01 00:00:00
abstract::Several selective media were evaluated for the primary isolation and enumeration of Staphylococcus aureus from halogenated indoor swimming pool waters. Standard plate counts of the viable population and total coliform densities were also determined to ascertain their value as indicator systems. All studies were done w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.4.699-702.1986
更新日期:1986-04-01 00:00:00
abstract::The cry19A operon of Bacillus thuringiensis subsp. jegathesan encodes two proteins, mosquitocidal Cry19A (ORF1; 75 kDa) and an ORF2 (60 kDa) of unknown function. Expression of the cry19A operon in an acrystalliferous strain of B. thuringiensis (4Q7) yielded one small crystal per cell, whereas no crystals were produced...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06750-11
更新日期:2012-03-01 00:00:00
abstract::Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin pr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.10.4468-4474.2000
更新日期:2000-10-01 00:00:00
abstract::Several transposable elements were isolated from the genome of Xanthomonas oryzae pv. oryzae. These elements and an avirulence gene isolated from X. oryzae pv. oryzae were used as hybridization probes for a collection of X. oryzae pv. oryzae strains from the Philippines. Each of the sequences was present in multiple c...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.9.3275-3283.1994
更新日期:1994-09-01 00:00:00
abstract::The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocy...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01777-06
更新日期:2006-12-01 00:00:00
abstract::A method for the measurement of specific levels of gene expression that combines target mRNA and target DNA quantitation has been developed. The use of target gene dose as a normalizing factor for mRNA provides an alternative to 16S or 23S rRNA, which are unsuitable for use in the environment because of their presence...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.2.451-457.1993
更新日期:1993-02-01 00:00:00
abstract::Although it is widely believed that horizontal patchiness exists in microbial sediment communities, determining the extent of variability or the particular members of the bacterial community which account for the observed differences among sites at various scales has not been routinely demonstrated. In this study, hor...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.5.1980-1986.2000
更新日期:2000-05-01 00:00:00
abstract::Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two ot...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.8.2095-2097.1989
更新日期:1989-08-01 00:00:00
abstract::Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same syst...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.2.465-467.1989
更新日期:1989-02-01 00:00:00
abstract::A mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. An organism, identified as Arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.5.1020-1025.1984
更新日期:1984-11-01 00:00:00
abstract::Strain VeGlc2, an anaerobic ultramicrobacterium belonging to the Verrucomicrobiales lineage of bacterial descent, fermented glucose to acetate, propionate, succinate, and CO2. The distribution of radiolabel in the fermentation end products produced from position-labelled glucose and in vitro measurements of enzyme act...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.12.4830-4833.1998
更新日期:1998-12-01 00:00:00
abstract::Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01591-13
更新日期:2013-10-01 00:00:00
abstract::Olavius crassitunicatus is a small symbiont-bearing worm that occurs at high abundance in oxygen-deficient sediments in the East Pacific Ocean. Using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization, we examined the diversity and phylogeny of bacterial symbionts in two geographically dista...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.3.1553-1561.2005
更新日期:2005-03-01 00:00:00
abstract::A recombinant Escherichia coli strain carrying a plasmid with an antibiotic resistance marker and expressing the green fluorescent protein was inoculated at a concentration of 3.8 x 10(8) CFU/g into direct-cut wheat (348 g of dry matter kg(-1)), wilted wheat (450 g of dry matter kg(-1)), and corn (375 g of dry matter ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.9.5163-5170.2005
更新日期:2005-09-01 00:00:00
abstract::Cytolysin and gelatinase are prominent pathogenicity determinants associated with highly virulent Enterococcus faecalis strains. In an effort to explore the expression profiles of these virulence traits in vivo, we have employed E. faecalis variants expressing the luxABCDE cassette under the control of either the P16S...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00831-13
更新日期:2013-07-01 00:00:00
abstract::Few techniques are currently available for quantifying specific prokaryotic taxa in environmental samples. Quantification of specific genotypes has relied mainly on oligonucleotide hybridization to extracted rRNA or intact rRNA in whole cells. However, low abundance and cellular rRNA content limit the application of t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.11.4605-4614.2000
更新日期:2000-11-01 00:00:00
abstract::The secondary metabolite mediating the GacS-dependent growth-inhibitory effect exerted by the rice rhizosphere isolate Pseudomonas putida RW10S2 on phytopathogenic Xanthomonas species was identified as white-line-inducing principle (WLIP), a member of the viscosin group of cyclic lipononadepsipeptides. WLIP producers ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00335-12
更新日期:2012-07-01 00:00:00
abstract::The cheese microbiota contributes to a large extent to the development of the typical color, flavor, and texture of the final product. Its composition is not well defined in most cases and varies from one cheese to another. The aim of the present study was to establish procedures for gene transcript quantification in ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02360-12
更新日期:2013-01-01 00:00:00
abstract::A competitive PCR technique was used to enumerate the proteolytic bacterium Clostridium proteoclasticum from the rumen. A PCR primer, which circumscribes this organism and several closely related strains, was designed for a variable region within their 16S rRNA genes and was used in conjunction with a universal forwar...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.3.907-913.1998
更新日期:1998-03-01 00:00:00
abstract::Bacterial precipitation of barite (BaSO(4)) under laboratory conditions is reported for the first time. The bacterium Myxococcus xanthus was cultivated in a solid medium with a diluted solution of barium chloride. Crystallization occurred as a result of the presence of live bacteria and the bacterial metabolic activit...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.9.5722-5725.2003
更新日期:2003-09-01 00:00:00
abstract::Organically raised swine had high fecal populations of chlortetracycline (CTC)-resistant (growing at 64 μg CTC/ml) Escherichia coli, Megasphaera elsdenii, and anaerobic bacteria. By comparison, CTC-resistant bacteria in feral swine feces were over 1,000-fold fewer and exhibited lower taxonomic diversity. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00688-11
更新日期:2011-10-01 00:00:00
abstract::Killer toxin activity of Pichia anomala WC65 appeared fungicidal for P. bimundalis WC38 and fungistatic for Candida albicans RC1. Inhibitory activity against sensitive C. albicans showed a linear relationship between toxin concentrations and the inverse of the reduced growth rates. The plot of toxin concentrations aga...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.5.1099-1103.1988
更新日期:1988-05-01 00:00:00
abstract::Wolbachia, a common bacterial endosymbiont of insects, has been shown to protect its hosts against a wide range of pathogens. However, not all strains exert a protective effect on their host. Here we assess the effects of two divergent Wolbachia strains, wAlbB from Aedes albopictus and wMelPop from Drosophila melanoga...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06751-11
更新日期:2012-03-01 00:00:00
abstract::During fermentative growth in natural and industrial environments, Saccharomyces cerevisiae must redistribute the available nitrogen from multiple exogenous sources to amino acids in order to suitably fulfill anabolic requirements. To exhaustively explore the management of this complex resource, we developed an advanc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02617-16
更新日期:2017-02-15 00:00:00
abstract::Noroviruses from mussels collected near sewage effluents were compared with local patient outbreak strains. Sequence analyses of RNA polymerase-capsid-poly(A)-3' (3.1-kilobase) regions confirmed the 99.9% similarity between genotype I.1 strains from mussels and patient strains from recreational-bathing outbreaks, indi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02477-07
更新日期:2008-04-01 00:00:00
abstract::The dairy starter bacterium Lactococcus lactis is able to synthesize folate and accumulates large amounts of folate, predominantly in the polyglutamyl form. Only small amounts of the produced folate are released in the extracellular medium. Five genes involved in folate biosynthesis were identified in a folate gene cl...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.6.3069-3076.2003
更新日期:2003-06-01 00:00:00
abstract::The growth characteristics and acetate production of several Escherichia coli strains were compared by using shake flasks, batch fermentations, and glucose-feedback-controlled fed-batch fermentations to assess the potential of each strain to grow at high cell densities. Of the E. coli strains tested, including JM105, ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.4.1004-1011.1990
更新日期:1990-04-01 00:00:00
abstract::A single gene (xylB) encoding both beta-D-xylosidase (EC 3.2.1.37) and alpha-L-arabinofuranosidase (EC 3.2.1.55) activities was identified and sequenced from the ruminal bacterium Butyrivibrio fibrisolvens. The xylB gene consists of a 1.551-bp open reading frame (ORF) encoding 517 amino acids. A subclone containing a ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.4.1227-1234.1991
更新日期:1991-04-01 00:00:00
abstract::Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.1.84-91.2003
更新日期:2003-01-01 00:00:00