Pathway of glucose catabolism by strain VeGlc2, an anaerobe belonging to the verrucomicrobiales lineage of bacterial descent

Abstract:

:Strain VeGlc2, an anaerobic ultramicrobacterium belonging to the Verrucomicrobiales lineage of bacterial descent, fermented glucose to acetate, propionate, succinate, and CO2. The distribution of radiolabel in the fermentation end products produced from position-labelled glucose and in vitro measurements of enzyme activities in crude cell extracts prepared from glucose-grown cells showed that glucose was metabolized via the Embden-Meyerhof-Parnas pathway. The 6-phosphofructokinase (EC 2.7.1.90) activity required pyrophosphate as the phosphoryl donor, and ATP could not replace pyrophosphate. The other enzyme activities were those of a classical Embden-Meyerhof-Parnas pathway. 14CO2 was incorporated into propionate and succinate, suggesting that a carboxylation reaction rather than a transcarboxylation reaction was involved in the reductive pathway leading to succinate and propionate. Difference spectra showed that a type b cytochrome was present, which could be involved in electron transport in the reductive pathway.

journal_name

Appl Environ Microbiol

authors

Janssen PH

doi

10.1128/AEM.64.12.4830-4833.1998

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

4830-3

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

64

pub_type

杂志文章
  • Effect of Macrolide and Rifampin Resistance on the Fitness of Rhodococcus equi.

    abstract::Rhodococcus equi is a leading cause of severe pneumonia in foals. Standard treatment is dual antimicrobial therapy with a macrolide and rifampin, but the emergence of macrolide- and rifampin-resistant R. equi isolates is an increasing problem. The objective of this study was to determine the effect of macrolide and/or...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02665-18

    authors: Willingham-Lane JM,Berghaus LJ,Berghaus RD,Hart KA,Giguère S

    更新日期:2019-03-22 00:00:00

  • Different contributions of HtrA protease and chaperone activities to Campylobacter jejuni stress tolerance and physiology.

    abstract::The microaerophilic bacterium Campylobacter jejuni is the most common cause of bacterial food-borne infections in the developed world. Tolerance to environmental stress relies on proteases and chaperones in the cell envelope, such as HtrA and SurA. HtrA displays both chaperone and protease activities, but little is kn...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01603-10

    authors: Baek KT,Vegge CS,Skórko-Glonek J,Brøndsted L

    更新日期:2011-01-01 00:00:00

  • Population dynamics of Bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry.

    abstract::The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01777-06

    authors: Dinoto A,Marques TM,Sakamoto K,Fukiya S,Watanabe J,Ito S,Yokota A

    更新日期:2006-12-01 00:00:00

  • Effect of coumarin on growth and metabolism of Pythium.

    abstract::Coumarin concentrations that inhibited growth of Pythium sp. rapidly decreased the rate of incorporation of [14C]glucose into the mycelium. Coumarin also reduced drastically the carbohydrate and protein content of the cytoplasm and, to a lesser extent, the amino acid content and cell wall fraction. Enzymes related to ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.3.258-262.1977

    authors: Neto FC,Dietrich SM

    更新日期:1977-09-01 00:00:00

  • Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase.

    abstract::Enzymes with high specific activities at low temperatures have potential uses for chemical conversions when low temperatures are required, as in the food industry. Psychrotrophic microorganisms which grow at low temperatures may be a valuable source of cold-active enzymes that have higher activities at low temperature...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.12.4544-4552.1994

    authors: Trimbur DE,Gutshall KR,Prema P,Brenchley JE

    更新日期:1994-12-01 00:00:00

  • A Vibrating Mesh Nebulizer as an Alternative to the Collison Three-Jet Nebulizer for Infectious Disease Aerobiology.

    abstract::Experimental infection of animals with aerosols containing pathogenic agents is essential for an understanding of the natural history and pathogenesis of infectious disease as well as evaluation of potential treatments. We evaluated whether the Aeroneb nebulizer, a vibrating mesh nebulizer, would serve as an alternati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00747-19

    authors: Bowling JD,O'Malley KJ,Klimstra WB,Hartman AL,Reed DS

    更新日期:2019-08-14 00:00:00

  • Shewanella decolorationis LDS1 Chromate Resistance.

    abstract::The genus Shewanella is well known for its genetic diversity, its outstanding respiratory capacity, and its high potential for bioremediation. Here, a novel strain isolated from sediments of the Indian Ocean was characterized. A 16S rRNA analysis indicated that it belongs to the species Shewanella decolorationis It wa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00777-19

    authors: Lemaire ON,Honoré FA,Tempel S,Fortier EM,Leimkühler S,Méjean V,Iobbi-Nivol C

    更新日期:2019-08-29 00:00:00

  • Bacterial fermentation of cheese whey for production of a ruminant feed supplement rich in curde protein.

    abstract::A simple and efficient process for the production of a ruminant feed supplement, rich in crude protein (defined as total N X 6.25), by bacterial fermentation of cheese whey has been developed. The lactose in unpasteurized whey is fermented to lactate acid by Lactobacillus bulgaricus at a temperature of 43 degrees C an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.6.769-776.1976

    authors: Reddy CA,Henderson HE,Erdman MD

    更新日期:1976-12-01 00:00:00

  • Bacterial degradation of emulsan.

    abstract::Emulsan is a polyanionic heteropolysaccharide bioemulsifier produced by Acinetobacter calcoaceticus RAG-1. A mixed bacterial population was obtained by enrichment culture that was capable of degrading emulsan and using it as a carbon source. From this mixed culture, an emulsan-degrading bacterium, termed YUV-1, was is...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.3.573-579.1983

    authors: Shoham Y,Rosenberg M,Rosenberg E

    更新日期:1983-09-01 00:00:00

  • Developmental and microbiological analysis of the inception of bioluminescent symbiosis in the marine fish Nuchequula nuchalis (Perciformes: Leiognathidae).

    abstract::Many marine fish harbor luminous bacteria as bioluminescent symbionts. Despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01619-08

    authors: Dunlap PV,Davis KM,Tomiyama S,Fujino M,Fukui A

    更新日期:2008-12-01 00:00:00

  • Distribution and survival of motile Aeromonas spp. in brackish water receiving sewage treatment effluent.

    abstract::The spatiotemporal distributions of Aeromonas spp. and fecal coliforms in a cove receiving sewage treatment effluent and draining into a brackish lagoon were studied for 34 months with sampling at six stations. A total of 452 strains of Aeromonas spp. were isolated and identified at the outflow of the treatment system...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2459-2467.1991

    authors: Monfort P,Baleux B

    更新日期:1991-09-01 00:00:00

  • Fluorescence correlation spectroscopy to study diffusion and reaction of bacteriophages inside biofilms.

    abstract::In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02304-07

    authors: Briandet R,Lacroix-Gueu P,Renault M,Lecart S,Meylheuc T,Bidnenko E,Steenkeste K,Bellon-Fontaine MN,Fontaine-Aupart MP

    更新日期:2008-04-01 00:00:00

  • Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis.

    abstract::Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.8.2551-2558.1990

    authors: Liu W,Hansen JN

    更新日期:1990-08-01 00:00:00

  • Role of filamentous phage SW1 in regulating the lateral flagella of Shewanella piezotolerans strain WP3 at low temperatures.

    abstract::Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01675-13

    authors: Jian H,Xiao X,Wang F

    更新日期:2013-11-01 00:00:00

  • Nonphotosynthetic pigmented bacteria in a potable water treatment and distribution system.

    abstract::The occurrence of pigmented bacteria in potable water, from raw source water through treatment to distribution water, including dead-end locations, was compared at sample sites in a large municipal water system. Media used to enumerate heterotrophic bacteria and differentiate pigmented colonies were standard method pl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.4.912-921.1989

    authors: Reasoner DJ,Blannon JC,Geldreich EE,Barnick J

    更新日期:1989-04-01 00:00:00

  • Typing Listeria monocytogenes isolates from fish products and human listeriosis cases.

    abstract::Seventy-two Listeria monocytogenes isolates originating from 10 different fish products of 12 producers and 47 isolates from human listeriosis cases were typed by serotyping and multilocus enzyme electrophoresis. Seventy-five of these isolates were further subtyped by restriction analysis of genomic DNA with the enzym...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.4.1338-1343.1997

    authors: Boerlin P,Boerlin-Petzold F,Bannerman E,Bille J,Jemmi T

    更新日期:1997-04-01 00:00:00

  • Nitrogen fixation by Vibrio parahaemolyticus and its implications for a new ecological niche.

    abstract::A Vibrio parahaemolyticus strain isolated from the rhizosphere of the ecosystem dominant estuarine grass, Spartina alterniflora, was characterized and shown to carry nifH, the gene encoding the nitrogenase iron protein, and to fix N(2). Nitrogen fixation may contribute substantially to the adaptability, niche breadth,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00981-07

    authors: Criminger JD,Hazen TH,Sobecky PA,Lovell CR

    更新日期:2007-09-01 00:00:00

  • Factors limiting the spread of the protective symbiont Hamiltonella defensa in Aphis craccivora Aphids.

    abstract::Many insects are associated with heritable symbionts that mediate ecological interactions, including host protection against natural enemies. The cowpea aphid, Aphis craccivora, is a polyphagous pest that harbors Hamiltonella defensa, which defends against parasitic wasps. Despite this protective benefit, this symbion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01775-14

    authors: Dykstra HR,Weldon SR,Martinez AJ,White JA,Hopper KR,Heimpel GE,Asplen MK,Oliver KM

    更新日期:2014-09-01 00:00:00

  • Antibiotic manipulation of intestinal microbiota to identify microbes associated with Campylobacter jejuni exclusion in poultry.

    abstract::The ability of various subsets of poultry intestinal microbiota to protect turkeys from colonization by Campylobacter jejuni was investigated. Community subsets were generated in vivo by inoculation of day-old poults with the cecal contents of a Campylobacter-free adult turkey, followed by treatment with one antimicro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00678-10

    authors: Scupham AJ,Jones JA,Rettedal EA,Weber TE

    更新日期:2010-12-01 00:00:00

  • Repetitive, marker-free, site-specific integration as a novel tool for multiple chromosomal integration of DNA.

    abstract::We present a tool for repetitive, marker-free, site-specific integration in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB). The novelty of the tool described here is the inclusion of a minimal bacteri...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00346-13

    authors: Petersen KV,Martinussen J,Jensen PR,Solem C

    更新日期:2013-06-01 00:00:00

  • Use of repetitive DNA sequences and the PCR To differentiate Escherichia coli isolates from human and animal sources.

    abstract::The rep-PCR DNA fingerprint technique, which uses repetitive intergenic DNA sequences, was investigated as a way to differentiate between human and animal sources of fecal pollution. BOX and REP primers were used to generate DNA fingerprints from Escherichia coli strains isolated from human and animal sources (geese, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.6.2572-2577.2000

    authors: Dombek PE,Johnson LK,Zimmerley ST,Sadowsky MJ

    更新日期:2000-06-01 00:00:00

  • Binding of virus-like particles of Norwalk virus to romaine lettuce veins.

    abstract::Noroviruses (NoV) annually cause millions of cases of gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which are thought to bioaccumulate NoV particles during the filter-feeding process. NoV outbreaks, however, have also been known to occur from othe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01566-10

    authors: Gandhi KM,Mandrell RE,Tian P

    更新日期:2010-12-01 00:00:00

  • Submicronic fungal bioaerosols: high-resolution microscopic characterization and quantification.

    abstract::Submicronic particles released from fungal cultures have been suggested to be additional sources of personal exposure in mold-contaminated buildings. In vitro generation of these particles has been studied with particle counters, eventually supplemented by autofluorescence, that recognize fragments by size and discrim...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01740-14

    authors: Afanou KA,Straumfors A,Skogstad A,Nilsen T,Synnes O,Skaar I,Hjeljord L,Tronsmo A,Green BJ,Eduard W

    更新日期:2014-11-01 00:00:00

  • Comparative transcriptome analysis of Listeria monocytogenes strains of the two major lineages reveals differences in virulence, cell wall, and stress response.

    abstract::Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02730-06

    authors: Severino P,Dussurget O,Vêncio RZ,Dumas E,Garrido P,Padilla G,Piveteau P,Lemaître JP,Kunst F,Glaser P,Buchrieser C

    更新日期:2007-10-01 00:00:00

  • Degradation of 1,2-dichloroethane by Ancylobacter aquaticus and other facultative methylotrophs.

    abstract::Cultures of the newly isolated bacterial strains AD20, AD25, and AD27, identified as strains of Ancylobacter aquaticus, were capable of growth on 1,2-dichloroethane (DCE) as the sole carbon and energy source. These strains, as well as two other new DCE utilizers, were facultative methylotrophs and were also able to gr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.3.976-983.1992

    authors: van den Wijngaard AJ,van der Kamp KW,van der Ploeg J,Pries F,Kazemier B,Janssen DB

    更新日期:1992-03-01 00:00:00

  • Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH.

    abstract::Alpha-ketoglutarate-dependent (R)-dichlorprop dioxygenase (RdpA) and alpha-ketoglutarate-dependent (S)-dichlorprop dioxygenase (SdpA), which are involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH, were expressed and purified as His6-tagged fusion proteins from Escherichia...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02758-05

    authors: Müller TA,Fleischmann T,van der Meer JR,Kohler HP

    更新日期:2006-07-01 00:00:00

  • Lactobacillus oligofermentans sp. nov., associated with spoilage of modified-atmosphere-packaged poultry products.

    abstract::Unidentified lactic acid bacterium (LAB) isolates which had mainly been detected in spoiled, marinated, modified atmosphere packaged (MAP) broiler meat products during two previous studies, were identified and analyzed for their phenotypic properties and the capability to produce biogenic amines. To establish the taxo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4400-4406.2005

    authors: Koort J,Murros A,Coenye T,Eerola S,Vandamme P,Sukura A,Björkroth J

    更新日期:2005-08-01 00:00:00

  • Tentative identification of methanogenic bacteria by fluorescence microscopy.

    abstract::Methanogenic bacteria, which are presently identified on the basis of cell morphology and substrate conversion to CH(4), can be differentiated from nonmethanogens and identified in pure or mixed culture on the basis of their autofluorescence under ultraviolet illumination. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.3.713-717.1977

    authors: Mink RW,Dugan PR

    更新日期:1977-03-01 00:00:00

  • A novel insecticidal toxin from photorhabdus luminescens, toxin complex a (Tca), and its histopathological effects on the midgut of manduca sexta

    abstract::Photorhabdus luminescens is a bacterium which is mutualistic with entomophagous nematodes and which secretes high-molecular-weight toxin complexes following its release into the insect hemocoel upon nematode invasion. Thus, unlike other protein toxins from Bacillus thuringiensis (delta-endotoxins and Vip's), P. lumine...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.8.3036-3041.1998

    authors: Blackburn M,Golubeva E,Bowen D,Ffrench-Constant RH

    更新日期:1998-08-01 00:00:00

  • Distribution of human virus contamination in shellfish from different growing areas in Greece, Spain, Sweden, and the United Kingdom.

    abstract::Viral pollution in shellfish has been analyzed simultaneously across a wide range of geographical regions, with emphasis on the concomitant variations in physicochemical characteristics and social features. The methods for sample treatment and for the detection of human enteric viruses were optimized by the participat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.12.5990-5998.2002

    authors: Formiga-Cruz M,Tofiño-Quesada G,Bofill-Mas S,Lees DN,Henshilwood K,Allard AK,Conden-Hansson AC,Hernroth BE,Vantarakis A,Tsibouxi A,Papapetropoulou M,Furones MD,Girones R

    更新日期:2002-12-01 00:00:00