7-Mercaptoheptanoylthreonine phosphate substitutes for heat-stable factor (mobile factor) for growth of Methanomicrobium mobile.

Abstract:

:Methanomicrobium mobile requires a heat-stable factor present in ruminal fluid and in boiled cell extract from Methanobacterium thermoautotrophicum for growth. By comparing the growth of M. mobile with boiled cell extract with that observed with various methanogenic cofactors, we found that 7-mercaptoheptanoylthreonine phosphate (HS-HTP) supported sustained growth of M. mobile, at an optimal concentration of 100 microM. No derivatives or possible biosynthetic precursors of HS-HTP could replace HS-HTP as the sole source of growth factor. Results suggest that the growth requirement might be satisfied by 7-mercaptoheptanoic acid plus a second, unidentified heat-stable factor.

journal_name

Appl Environ Microbiol

authors

Kuhner CH,Smith SS,Noll KM,Tanner RS,Wolfe RS

doi

10.1128/AEM.57.10.2891-2895.1991

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

2891-5

issue

10

eissn

0099-2240

issn

1098-5336

journal_volume

57

pub_type

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