Abstract:
:The medicinal value associated with complex polyketide and nonribosomal peptide natural products has prompted biosynthetic schemes dependent upon heterologous microbial hosts. Here we report the successful biosynthesis of yersiniabactin (Ybt), a model polyketide-nonribosomal peptide hybrid natural product, using Escherichia coli as a heterologous host. After introducing the biochemical pathway for Ybt into E. coli, biosynthesis was initially monitored qualitatively by mass spectrometry. Next, production of Ybt was quantified in a high-cell-density fermentation environment with titers reaching 67 +/- 21 (mean +/- standard deviation) mg/liter and a volumetric productivity of 1.1 +/- 0.3 mg/liter-h. This success has implications for basic and applied studies on Ybt biosynthesis and also, more generally, for future production of polyketide, nonribosomal peptide, and mixed polyketide-nonribosomal peptide natural products using E. coli.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Pfeifer BA,Wang CC,Walsh CT,Khosla Cdoi
10.1128/aem.69.11.6698-6702.2003keywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
6698-702issue
11eissn
0099-2240issn
1098-5336journal_volume
69pub_type
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