Increases in the amounts of Vibrio spp. in oysters upon addition of exogenous bacteria.

Abstract:

:The bacterial pathogen Vibrio vulnificus is found naturally in brackish coastal waters but can be greatly concentrated by filter-feeding organisms such as shellfish. Numerous experiments in which exogenous V. vulnificus cells are added to oysters in an attempt to measure uptake and depuration have been performed. In nearly all cases, results have shown that laboratory-grown bacteria are rapidly taken up by the oysters but ultimately eliminated, while naturally present Vibrio populations in oysters are resistant to depuration. In this study, oysters harvested during winter months, with low culturable Vibrio concentrations, were incubated in aquaria supplemented with strains of V. vulnificus that were either genotypically or phenotypically distinct from the background bacteria. These exogenous cells were eliminated from the oysters, as previously seen, but other vibrios already inhabiting the oysters responded to the V. vulnificus inoculum by rapidly increasing in number and maintaining a large stable population. The presence of such an oyster-adapted Vibrio population would be expected to prevent colonization by exogenous V. vulnificus cells, thus explaining the rapid depuration of these added bacteria.

journal_name

Appl Environ Microbiol

authors

Froelich B,Oliver J

doi

10.1128/AEM.01110-13

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

5208-13

issue

17

eissn

0099-2240

issn

1098-5336

pii

AEM.01110-13

journal_volume

79

pub_type

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