Abstract:
:Minute amounts of oxygen were supplied to a continuous cultivation of Lactococcus lactis subsp. cremoris MG1363 grown on a defined glucose-limited medium at a dilution rate of 0.1 h(-1). More than 80% of the carbon supplied with glucose ended up in fermentation products other than lactate. Addition of even minute amounts of oxygen increased the yield of biomass on glucose by more than 10% compared to that obtained under anaerobic conditions and had a dramatic impact on catabolic enzyme activities and hence on the distribution of carbon at the pyruvate branch point. Increasing aeration caused carbon dioxide and acetate to replace formate and ethanol as catabolic end products while hardly affecting the production of either acetoin or lactate. The negative impact of oxygen on the synthesis of pyruvate formate lyase was confirmed. Moreover, oxygen was shown to down regulate the protein level of alcohol dehydrogenase while increasing the enzyme activity levels of the pyruvate dehydrogenase complex, alpha-acetolactate synthase, and the NADH oxidases. Lactate dehydrogenase and glyceraldehyde dehydrogenase enzyme activity levels were unaffected by aeration.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Jensen NB,Melchiorsen CR,Jokumsen KV,Villadsen Jdoi
10.1128/AEM.67.6.2677-2682.2001keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
2677-82issue
6eissn
0099-2240issn
1098-5336journal_volume
67pub_type
杂志文章abstract::Campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. This procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of C. jejuni in milk from bulk tanks. ...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.46.2.459-462.1983
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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更新日期:2011-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1985-07-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.42.2.308-316.1981
更新日期:1981-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.56.8.2488-2493.1990
更新日期:1990-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.9.2203-2207.1988
更新日期:1988-09-01 00:00:00
abstract::A novel death-specific gene, ScDSP, was obtained from a death stage subtraction cDNA library of the diatom Skeletonema costatum. The full length of ScDSP cDNA was 921 bp in length, containing a 699-bp open reading frame encoding 232 amino acids and two stretches of 66 and 156 bp in the 5' and 3' untranslated regions, ...
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更新日期:2005-12-01 00:00:00
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更新日期:2003-04-01 00:00:00
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更新日期:1994-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.2.555-559.2002
更新日期:2002-02-01 00:00:00