Conjugal transfer of a toxin-coding megaplasmid from Bacillus thuringiensis subsp. israelensis to mosquitocidal strains of Bacillus sphaericus.

Abstract:

:Both Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis produce mosquitocidal toxins during sporulation and are extensively used in the field for control of mosquito populations. All the known toxins of the latter organism are known to be encoded on a large plasmid, pBtoxis. In an attempt to combine the best properties of the two bacteria, an erythromycin resistance-marked pBtoxis plasmid was transferred to B. sphaericus by a mating technique. The resulting transconjugant bacteria were significantly more toxic to Aedes aegypti mosquitoes and were able to overcome resistance to B. sphaericus in a resistant colony of Culex quinquefasciatus, apparently due to the production of Cry11A but not Cry4A or Cry4B. The stability of the plasmid in the B. sphaericus host was moderate during vegetative growth, but segregational instability was observed, which led to substantial rates of plasmid loss during sporulation.

journal_name

Appl Environ Microbiol

authors

Gammon K,Jones GW,Hope SJ,de Oliveira CM,Regis L,Silva Filha MH,Dancer BN,Berry C

doi

10.1128/AEM.72.3.1766-1770.2006

keywords:

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

1766-70

issue

3

eissn

0099-2240

issn

1098-5336

pii

72/3/1766

journal_volume

72

pub_type

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