Abstract:
:Both Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis produce mosquitocidal toxins during sporulation and are extensively used in the field for control of mosquito populations. All the known toxins of the latter organism are known to be encoded on a large plasmid, pBtoxis. In an attempt to combine the best properties of the two bacteria, an erythromycin resistance-marked pBtoxis plasmid was transferred to B. sphaericus by a mating technique. The resulting transconjugant bacteria were significantly more toxic to Aedes aegypti mosquitoes and were able to overcome resistance to B. sphaericus in a resistant colony of Culex quinquefasciatus, apparently due to the production of Cry11A but not Cry4A or Cry4B. The stability of the plasmid in the B. sphaericus host was moderate during vegetative growth, but segregational instability was observed, which led to substantial rates of plasmid loss during sporulation.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Gammon K,Jones GW,Hope SJ,de Oliveira CM,Regis L,Silva Filha MH,Dancer BN,Berry Cdoi
10.1128/AEM.72.3.1766-1770.2006keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
1766-70issue
3eissn
0099-2240issn
1098-5336pii
72/3/1766journal_volume
72pub_type
杂志文章abstract::This study presents population analyses of microbial communities inhabiting a site of extreme acid mine drainage (AMD) production. The site is the inactive underground Richmond mine at Iron Mountain, Calif., where the weathering of a massive sulfide ore body (mostly pyrite) produces solutions with pHs of approximately...
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更新日期:1980-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-05-01 00:00:00
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更新日期:1982-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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