Methods for enhanced culture recovery of Francisella tularensis.

abstract：:The polymerase chain reaction (PCR) and a radiolabeled oligonucleotide probe were used to specifically detect proteolytic and nonproteolytic Clostridium botulinum type B. Two synthetic primers deduced from the amino acid sequence data of type B neurotoxin were used to amplify a 1.5-kbp fragment corresponding to the li...

journal_title：Applied and environmental microbiology

authors： Szabo EA,Pemberton JM,Desmarchelier PM

更新日期：1992-01-01 00:00:00

abstract：:To assess the relative merits of tryptone yeast extract agar, the same medium unbuffered, and medium M17 for the assay of nine bacteriophages of lactic streptococci, comparative plaque counts were made with an overlay of 3 or 9 ml. Four of the phages exhibited no significant difference in plating efficiency between me...

journal_title：Applied and environmental microbiology

authors： Keogh BP

更新日期：1980-10-01 00:00:00

abstract：:Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days...

journal_title：Applied and environmental microbiology

authors： Chrisope GL,Fox CW,Marshall RT

更新日期：1976-05-01 00:00:00

abstract：:Basidiomycetes that cause brown rot of wood are essential biomass recyclers in coniferous forest ecosystems and a major cause of failure in wooden structures. Recent work indicates that distinct lineages of brown rot fungi have arisen independently from ligninolytic white rot ancestors via loss of lignocellulolytic en...

journal_title：Applied and environmental microbiology

authors： Korripally P,Timokhin VI,Houtman CJ,Mozuch MD,Hammel KE

更新日期：2013-04-01 00:00:00

abstract：:Statistical models were used to predict the effects of tryptone, glucose, yeast extract (TGY) and Mn on biomass formation of the highly radioresistant bacterium Deinococcus radiodurans. Results suggested that glucose had marginal effect on biomass buildup, but Mn was a significant factor for biomass formation. Mn also...

journal_title：Applied and environmental microbiology

authors： Zhang YM,Wong TY,Chen LY,Lin CS,Liu JK

更新日期：2000-01-01 00:00:00

abstract：:The kinetics of oxygen reduction by Desulfovibrio salexigens Mast1 and the role of polyglucose in this activity were examined and compared with those of strains of D. desulfuricans and D. gigas. Oxidation rates were highest at air saturation (up to 40 nmol of O(2) min mg of protein) and declined with decreasing oxygen...

journal_title：Applied and environmental microbiology

authors： van Niel EW,Gottschal JC

更新日期：1998-03-01 00:00:00

abstract：:Microscopic techniques were used in conjunction with normal microbiological procedures to examine the development of the spoilage microflora on the skin of chicken carcasses held at 2 degrees C. Pigmented and nonpigmented psychrotrophic pseudomonads were the major spoilage bacteria isolated at all stages of storage ex...

journal_title：Applied and environmental microbiology

authors： Thomas CJ,McMeekin TA

更新日期：1981-02-01 00:00:00

abstract：:Enterotoxin B produced by Staphylococus aureus 243 in brain heart infusion broth was concentrated by dialysis against 40% polyethylene glycol (20 M), partially purified on a Sephadex G-100 column and heated at 110 degrees C in thermal death time cans. Various heating menstrua included 0.04 M Veronal buffer (pH 7.4), b...

journal_title：Applied and environmental microbiology

authors： Lee IC,Stevenson KE,Harmon LG

更新日期：1977-02-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are microbial polyesters that can be used as completely biodegradable polymers, but the high production cost prevents their use in a wide range of applications. Recombinant Escherichia coli strains harboring the Ralstonia eutropha PHA biosynthesis genes have been reported to have several a...

journal_title：Applied and environmental microbiology

authors： Choi JI,Lee SY,Han K

更新日期：1998-12-01 00:00:00

abstract：:Different nutrient receptors varied in triggering germination of Bacillus subtilis spores with a pressure of 150 MPa, the GerA receptor being more responsive than the GerB receptor and even more responsive than the GerK receptor. This hierarchy in receptor responsiveness to pressure was the same as receptor responsive...

journal_title：Applied and environmental microbiology

authors： Black EP,Koziol-Dube K,Guan D,Wei J,Setlow B,Cortezzo DE,Hoover DG,Setlow P

更新日期：2005-10-01 00:00:00

abstract：:Fusarium oxysporum isolated from roots of and soil around Baccharis species from Brazil produced the trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and 3'-OH T-2 (TC-1), whereas Fusarium sporotrichioides from the same source produced T-2 toxin, HT-2 toxin, acetyl T-2, neosolaniol, TC-1, 3'-OH HT-2 (TC-3), i...

journal_title：Applied and environmental microbiology

authors： Mirocha CJ,Abbas HK,Kommedahl T,Jarvis BB

更新日期：1989-01-01 00:00:00

abstract：:The vertical and seasonal distribution and diversity of archaeal sequences was investigated in a hypersaline, stratified, monomictic lake, Solar Lake, Sinai, Egypt, during the limnological development of stratification and mixing. Archaeal sequences were studied via phylogenetic analysis of 16S rDNA sequences as well ...

journal_title：Applied and environmental microbiology

authors： Cytryn E,Minz D,Oremland RS,Cohen Y

更新日期：2000-08-01 00:00:00

abstract：:To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sa...

journal_title：Applied and environmental microbiology

authors： Ishii S,Kitamura G,Segawa T,Kobayashi A,Miura T,Sano D,Okabe S

更新日期：2014-12-01 00:00:00

abstract：:The frequency of Escherichia coli infection has lead to concerns over pathogenic bacteria in our food supply and a demand for therapeutics. Glycolipids on gut cells serve as receptors for the Shiga-like toxin produced by E. coli. Oligosaccharide moiety analogues of these glycolipids can compete with receptors for the ...

journal_title：Applied and environmental microbiology

authors： Liu Z,Lu Y,Zhang J,Pardee K,Wang PG

更新日期：2003-04-01 00:00:00

abstract：:Yeasts grown in the presence of benzoic acid tolerated 40 to 100% higher benzoic acid concentrations than did those grown in the absence of weak-acid-type preservatives. They also accumulated less benzoate in the presence of glucose. In chemostat cultures, benzoic acid reduced growth yield and the rate of cell product...

journal_title：Applied and environmental microbiology

authors： Warth AD

更新日期：1988-08-01 00:00:00

abstract：:The mercury resistance (mer) operon of plasmid R100 was cloned onto various plasmid vectors to study the effect of mer gene amplification on the rate of Hg2+ reduction by Escherichia coli cells. The plasmids were maintained at copy numbers ranging from 3 to 140 copies per cell. The overall Hg2+ reduction rate of intac...

journal_title：Applied and environmental microbiology

authors： Philippidis GP,Malmberg LH,Hu WS,Schottel JL

更新日期：1991-12-01 00:00:00

abstract：:The major gut bacteria of the worker caste of nine species of Australian termites, belonging to four families, were isolated and identified to generic level. All species were either facultative anaerobes or strict aerobes. A correlation appears to exist between the major gut bacterium and the family to which the termi...

journal_title：Applied and environmental microbiology

authors： Eutick ML,O'Brien RW,Slaytor M

更新日期：1978-05-01 00:00:00

abstract：:Recent declines in bumble bee populations are of great concern and have prompted critical evaluations of the role of pathogen introductions and host resistance in bee health. One factor that may influence host resilience when facing infection is the gut microbiota. Previous experiments with Bombus terrestris, a Europe...

journal_title：Applied and environmental microbiology

authors： Mockler BK,Kwong WK,Moran NA,Koch H

更新日期：2018-03-19 00:00:00

abstract：:Growth inhibition by isoleucine hydroxamate in Serratia marcescens was significantly enhanced by adding valine plus leucine and by using glycerol as the carbon source. Isoleucine hydroxamate-resistant mutants were isolated under conditions in which growth inhibition was enhanced. One of the mutants, strain GIHVLr2179,...

journal_title：Applied and environmental microbiology

authors： Kisumi M,Komatsubara S,Chibata I

更新日期：1977-12-01 00:00:00

abstract：:The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global a...

journal_title：Applied and environmental microbiology

authors： Yee N,Ma J,Dalia A,Boonfueng T,Kobayashi DY

更新日期：2007-03-01 00:00:00

abstract：:The present investigation showed by means of autoradiography that the cyanobacterium Microcystis wesenbergii did not incorporate [H]thymidine at nanomolar concentrations, whereas its associated heterotrophic bacteria appearing in the gelatinous cover of the cyanobacterium became labeled. Several other tested cyaobacte...

journal_title：Applied and environmental microbiology

authors： Bern L

更新日期：1985-01-01 00:00:00

abstract：:The reliable detection of airborne biological threat agents depends on several factors, including the performance criteria of the detector and its operational environment. One step in improving the detector's performance is to increase our knowledge of the biological aerosol background in potential operational environ...

journal_title：Applied and environmental microbiology

authors： Dybwad M,Granum PE,Bruheim P,Blatny JM

更新日期：2012-03-01 00:00:00

abstract：:Homologous recombination plays an important role in the structuring of genetic variation of many bacteria; however, its importance in adaptive evolution is not well established. We investigated the association of intersubspecific homologous recombination (IHR) with the shift to a novel host (mulberry) by the plant-pat...

journal_title：Applied and environmental microbiology

authors： Nunney L,Schuenzel EL,Scally M,Bromley RE,Stouthamer R

更新日期：2014-05-01 00:00:00

abstract：:Aerobic biotransformation of the diaryl ethers 2,7-dichlorodibenzo-p-dioxin and 1,2,3,4-tetrachlorodibenzo-p-dioxin by the dibenzo-p-dioxin-utilizing strain Sphingomonas wittichii RW1, producing corresponding metabolites, was demonstrated for the first time. Our strain transformed 2,7-dichlorodibenzo-p-dioxin, yieldin...

journal_title：Applied and environmental microbiology

authors： Hong HB,Chang YS,Nam IH,Fortnagel P,Schmidt S

更新日期：2002-05-01 00:00:00

abstract：:Efficient biosynthesis of L-tyrosine from glucose is necessary to make biological production economically viable. To this end, we designed and constructed a modular biosynthetic pathway for L-tyrosine production in E. coli MG1655 by encoding the enzymes for converting erythrose-4-phosphate (E4P) and phosphoenolpyruvat...

journal_title：Applied and environmental microbiology

authors： Juminaga D,Baidoo EE,Redding-Johanson AM,Batth TS,Burd H,Mukhopadhyay A,Petzold CJ,Keasling JD

更新日期：2012-01-01 00:00:00

abstract：:The genus of Marinobacter is one of the most ubiquitous in the global oceans and assumed to significantly impact various biogeochemical cycles. The genome structure and content of Marinobacter aquaeolei VT8 was analyzed and compared with those from other organisms with diverse adaptive strategies. Here, we report the ...

journal_title：Applied and environmental microbiology

authors： Singer E,Webb EA,Nelson WC,Heidelberg JF,Ivanova N,Pati A,Edwards KJ

更新日期：2011-04-01 00:00:00

abstract：:One emerging disease of grapevine in Europe is Bois noir (BN), a phytoplasmosis caused by "Candidatus Phytoplasma solani" and spread in vineyards by the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). Here we present the first full characterization of the bacterial community of this important disease vector co...

journal_title：Applied and environmental microbiology

authors： Gonella E,Negri I,Marzorati M,Mandrioli M,Sacchi L,Pajoro M,Crotti E,Rizzi A,Clementi E,Tedeschi R,Bandi C,Alma A,Daffonchio D

更新日期：2011-02-01 00:00:00

abstract：:A total of 69 dyes were incorporated separately at different concentrations into an agar medium for evaluation of their effects on the quantitative recovery of five serotypes of Yersinia enterocolitica. One strain of Pseudomonas aeruginosa and one strain of Bacillus cereus were included for comparative purposes. Certa...

journal_title：Applied and environmental microbiology

authors： Schiemann DA

更新日期：1979-08-01 00:00:00

abstract：:The cry19A operon of Bacillus thuringiensis subsp. jegathesan encodes two proteins, mosquitocidal Cry19A (ORF1; 75 kDa) and an ORF2 (60 kDa) of unknown function. Expression of the cry19A operon in an acrystalliferous strain of B. thuringiensis (4Q7) yielded one small crystal per cell, whereas no crystals were produced...

journal_title：Applied and environmental microbiology

authors： Barboza-Corona JE,Park HW,Bideshi DK,Federici BA

更新日期：2012-03-01 00:00:00

abstract：:We investigated the effect of preincubation of environmental waters amended with inorganic nutrients (nitrogen, phosphorus, and traces of iron and magnesium) on the kinetics of the microbial transformation of phenol, propanil, propyl ester of (2,4-dichlorophenoxy)acetic acid, methyl parathion, Ronnel, and methoxychlor...

journal_title：Applied and environmental microbiology

authors： Paris DF,Rogers JE

更新日期：1986-02-01 00:00:00