Abstract:
:A lambda recombinant phage expressing beta-mannanase activity in Escherichia coli has been isolated from a genomic library of the extremely thermophilic anaerobe "Caldocellum saccharolyticum." The gene was cloned into pBR322 on a 5-kb BamHI fragment, and its location was obtained by deletion analysis. The sequence of a 2.1-kb fragment containing the mannanase gene has been determined. One open reading frame was found which could code for a protein of Mr 38,904. The mannanase gene (manA) was overexpressed in E. coli by cloning the gene downstream from the lacZ promoter of pUC18. The enzyme was most active at pH 6 and 80 degrees C and degraded locust bean gum, guar gum, Pinus radiata glucomannan, and konjak glucomannan. The noncoding region downstream from the mannanase gene showed strong homology to celB, a gene coding for a cellulase from the same organism, suggesting that the manA gene might have been inserted into its present position on the "C. saccharolyticum" genome by homologous recombination.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Lüthi E,Jasmat NB,Grayling RA,Love DR,Bergquist PLdoi
10.1128/AEM.57.3.694-700.1991subject
Has Abstractpub_date
1991-03-01 00:00:00pages
694-700issue
3eissn
0099-2240issn
1098-5336journal_volume
57pub_type
杂志文章abstract::[This corrects the article on p. 662 in vol. 45.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.6.1964-1964.1983
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.2.900-912.2004
更新日期:2004-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.9.3924-3930.2000
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.5.1067-1071.1985
更新日期:1985-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.2.459-462.1983
更新日期:1983-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.11.5718-5727.2002
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.10.2394-2396.1987
更新日期:1987-10-01 00:00:00
abstract::A strain of the aerobic anoxygenic photosynthetic bacteria was isolated from a deep-ocean hydrothermal vent plume environment. The in vivo absorption spectra of cells indicate the presence of bacteriochlorophyll a incorporated into light-harvesting complex I and a reaction center. The general morphological and physiol...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.1.337-341.1998
更新日期:1998-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.6.1938-1942.1993
更新日期:1993-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
doi:10.1128/AEM.48.3.504-507.1984
更新日期:1984-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2016-12-15 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1988-10-01 00:00:00