Vibrio harveyi nitroreductase is also a chromate reductase.


:The chromate reductase purified from Pseudomonas ambigua was found to be homologous with several nitroreductases. Escherichia coli DH5alpha and Vibrio harveyi KCTC 2720 nitroreductases were chosen for the present study, and their chromate-reducing activities were determined. A fusion between glutathione S-transferase (GST) and E. coli DH5alpha NfsA (GST-EcNfsA), a fusion between GST and E. coli DH5alpha NfsB (GST-EcNfsB), and a fusion between GST and V. harveyi KCTC 2720 NfsA (GST-VhNfsA) were prepared for their overproduction and easy purification. GST-EcNfsA, GST-EcNFsB, and GST-VhNFsA efficiently reduced nitrofurazone and 2,4,6-trinitrotoluene (TNT) as their nitro substrates. The K(m) values for GST-EcNfsA, GST-EcNfsB, and GST-VhNfsA for chromate reduction were 11.8, 23.5, and 5.4 micro M, respectively. The V(max) values for GST-EcNfsA, GST-EcNfsB, and GST-VhNfsA were 3.8, 3.9, and 10.7 nmol/min/mg of protein, respectively. GST-VhNfsA was the most effective of the three chromate reductases, as determined by each V(max)/K(m) value. The optimal temperatures of GST-EcNfsA, GST-EcNfsB, and GST-VhNfsA for chromate reduction were 55, 30, and 30 degrees C, respectively. Thus, it is confirmed that nitroreductase can also act as a chromate reductase. Nitroreductases may be used in chromate remediation. GST-EcNfsA, GST-EcNfsB, and GST-VhNfsA have a molecular mass of 50 kDa and exist as a monomer in solution. Thin-layer chromatography showed that GST-EcNfsA, GST-EcNfsB, and GST-VhNfsA contain FMN as a cofactor. GST-VhNfsA reduced Cr(VI) to Cr(III). Cr(III) was much less toxic to E. coli than Cr(VI).


Appl Environ Microbiol


Kwak YH,Lee DS,Kim HB





Has Abstract


2003-08-01 00:00:00












  • Effect of Leuconostoc mesenteroides NRRL B-512F dextransucrase carboxy-terminal deletions on dextran and oligosaccharide synthesis.

    abstract::Dextransucrase (DSR-S) from Leuconostoc mesenteroides NRRL B-512F is a glucosyltransferase that catalyzes synthesis of soluble dextran from sucrose. In the presence of efficient acceptor molecules, such as maltose, the reaction pathway is shifted toward glucooligosaccharide synthesis. Like glucosyltransferases from or...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Monchois V,Reverte A,Remaud-Simeon M,Monsan P,Willemot RM

    更新日期:1998-05-01 00:00:00

  • Evidence from liposome encapsulation for transport-limited microbial metabolism of solid alkanes.

    abstract::The recalcitrance of xenobiotics may be caused by an absence of transforming enzymes or by their inability to enter microbial cells. A nondestructive method for differentiating between these two possibilities is described. The solid n-alkanes octadecane (C(18)) and hexatriacontane (C(36)) were encapsulated into phosph...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Miller RM,Bartha R

    更新日期:1989-02-01 00:00:00

  • Biosurfactant production by a soil pseudomonas strain growing on polycyclic aromatic hydrocarbons.

    abstract::The capacity of polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria to produce biosurfactants was investigated. Twenty-three bacteria isolated from a soil contaminated with petroleum wastes were able to form clearing zones on mineral salt agar plates sprayed with solutions of PAHs. Naphthalene and phenanthrene we...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Deziel E,Paquette G,Villemur R,Lepine F,Bisaillon J

    更新日期:1996-06-01 00:00:00

  • Campylobacter jejuni strains compete for colonization in broiler chicks.

    abstract::Campylobacter jejuni isolates possess multiple adhesive proteins termed adhesins, which promote the organism's attachment to epithelial cells. Based on the proposal that one or more adhesins are shared among C. jejuni isolates, we hypothesized that C. jejuni strains would compete for intestinal and cecal colonization ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Konkel ME,Christensen JE,Dhillon AS,Lane AB,Hare-Sanford R,Schaberg DM,Larson CL

    更新日期:2007-04-01 00:00:00

  • Thermal inactivation of susceptible and multiantimicrobial-resistant salmonella strains grown in the absence or presence of glucose.

    abstract::The heat resistance of susceptible and multiantimicrobial-resistant Salmonella strains grown to stationary phase in glucose-free tryptic soy broth supplemented with 0.6% yeast extract (TSBYE-G; nonadapted), in regular (0.25% glucose) TSBYE, or in TSBYE-G with 1.00% added glucose (TSBYE+G; acid adapted) was determined ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bacon RT,Ransom JR,Sofos JN,Kendall PA,Belk KE,Smith GC

    更新日期:2003-07-01 00:00:00

  • Biochemical fingerprinting of water coliform bacteria, a new method for measuring phenotypic diversity and for comparing different bacterial populations.

    abstract::A simple, automated microplate system for biochemical characterization of water isolates can be used to obtain fingerprints of the bacterial flora from various water samples. Mathematical models for calculating the diversities and similarities between bacterial populations are described for such fingerprints. The dive...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kühn I,Allestam G,Stenström TA,Möllby R

    更新日期:1991-11-01 00:00:00

  • Periplasmic cytochrome c3 of Desulfovibrio vulgaris is directly involved in H2-mediated metal but not sulfate reduction.

    abstract::Kinetic parameters and the role of cytochrome c(3) in sulfate, Fe(III), and U(VI) reduction were investigated in Desulfovibrio vulgaris Hildenborough. While sulfate reduction followed Michaelis-Menten kinetics (K(m) = 220 micro M), loss of Fe(III) and U(VI) was first-order at all concentrations tested. Initial reducti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Elias DA,Suflita JM,McInerney MJ,Krumholz LR

    更新日期:2004-01-01 00:00:00

  • Starvation-induced thermal tolerance as a survival mechanism in a psychrophilic marine bacterium.

    abstract::Carbon-starved cultures of strain Ant-300, a psychrophilic marine vibrio isolated from the Antarctic Convergence, were compared with their nonstarved counterparts for resistance to heat. Specifically, starved and unstarved cells were exposed to 17 degrees C, which is 4 degrees C above the maximum growth temperature, a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Preyer JM,Oliver JD

    更新日期:1993-08-01 00:00:00

  • Lyophilization prior to direct DNA extraction from bovine feces improves the quantification of Escherichia coli O157:H7 and Campylobacter jejuni.

    abstract::Lyophilization was used to concentrate bovine feces prior to DNA extraction and analysis using real-time PCR. Lyophilization significantly improved the sensitivity of detection compared to that in fresh feces and was associated with reliable quantification of both Escherichia coli O157:H7 and Campylobacter jejuni bact...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Rapp D,Waller J,Brightwell G,Muirhead RW

    更新日期:2010-03-01 00:00:00

  • Characterization of mundticin L, a class IIa anti-Listeria bacteriocin from Enterococcus mundtii CUGF08.

    abstract::Enterococcus mundtii CUGF08, a lactic acid bacterium isolated from alfalfa sprouts, was found to produce mundticin L, a new class IIa bacteriocin that has a high level of inhibitory activity against the genus Listeria. The plasmid-associated operons containing genes for the mundticin L precursor, the ATP binding casse...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Feng G,Guron GK,Churey JJ,Worobo RW

    更新日期:2009-09-01 00:00:00

  • Effect of Increasing the Copy Number of Bacteriophage Origins of Replication, in trans, on Incoming-Phage Proliferation.

    abstract::Bacteriophage resistance mechanisms which are derived from a bacteriophage genome are termed Per (phage-encoded resistance). When present in trans in Lactococcus lactis NCK203, Per50, the cloned origin of replication from phage phi50, interferes with phi50 replication. The per50 fragment was found to afford negligible...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: O'sullivan DJ,Hill C,Klaenhammer TR

    更新日期:1993-08-01 00:00:00

  • Isolation of tellurite- and selenite-resistant bacteria from hydrothermal vents of the Juan de Fuca Ridge in the Pacific Ocean.

    abstract::Deep-ocean hydrothermal-vent environments are rich in heavy metals and metalloids and present excellent sites for the isolation of metal-resistant microorganisms. Both metalloid-oxide-resistant and metalloid-oxide-reducing bacteria were found. Tellurite- and selenite-reducing strains were isolated in high numbers from...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Rathgeber C,Yurkova N,Stackebrandt E,Beatty JT,Yurkov V

    更新日期:2002-09-01 00:00:00

  • Anaerobic biodegradation of cyanide under methanogenic conditions.

    abstract::Upflow, anaerobic, fixed-bed, activated charcoal biotreatment columns capable of operating at free cyanide concentrations of greater than 100 mg liter-1 with a hydraulic retention time of less than 48 h were developed. Methanogenesis was maintained under a variety of feed medium conditions which included ethanol, phen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Fallon RD,Cooper DA,Speece R,Henson M

    更新日期:1991-06-01 00:00:00

  • Chloroethene biodegradation in sediments at 4 degrees C.

    abstract::Microbial reductive dechlorination of [1,2-14C]trichloroethene to [14C]cis-dichloroethene and [14C]vinyl chloride was observed at 4 degrees C in anoxic microcosms prepared with cold temperature-adapted aquifer and river sediments from Alaska. Microbial anaerobic oxidation of [1,2-14C]cis-dichloroethene and [1,2-14C]vi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bradley PM,Richmond S,Chapelle FH

    更新日期:2005-10-01 00:00:00

  • Persistence and differential survival of fecal indicator bacteria in subtropical waters and sediments.

    abstract::Fecal coliforms and enterococci are indicator organisms used worldwide to monitor water quality. These bacteria are used in microbial source tracking (MST) studies, which attempt to assess the contribution of various host species to fecal pollution in water. Ideally, all strains of a given indicator organism (IO) woul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Anderson KL,Whitlock JE,Harwood VJ

    更新日期:2005-06-01 00:00:00

  • Effects of organic substrates on dechlorination of aroclor 1242 in anaerobic sediments.

    abstract::The effects of different organic substrates on the abilities of anaerobic sediment enrichments to reductively dechlorinate polychlorinated biphenyls (PCBs) were studied. Sediments collected from a site previously contaminated with PCBs were dosed with additional PCBs (Aroclor 1242; approximately 300 ppm [300 mug/g], s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Nies L,Vogel TM

    更新日期:1990-09-01 00:00:00

  • Dynamics of Photosynthesis in a Glycogen-Deficient glgC Mutant of Synechococcus sp. Strain PCC 7002.

    abstract::Cyanobacterial glycogen-deficient mutants display impaired degradation of light-harvesting phycobilisomes under nitrogen-limiting growth conditions and secrete a suite of organic acids as a putative reductant-spilling mechanism. This genetic background, therefore, represents an important platform to better understand ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Jackson SA,Eaton-Rye JJ,Bryant DA,Posewitz MC,Davies FK

    更新日期:2015-09-01 00:00:00

  • Use of Raman Spectroscopy and Phase-Contrast Microscopy To Characterize Cold Atmospheric Plasma Inactivation of Individual Bacterial Spores.

    abstract:UNLABELLED:Raman spectroscopy and phase-contrast microscopy were used to examine calcium dipicolinate (CaDPA) levels and rates of nutrient and nonnutrient germination of multiple individual Bacillus subtilis spores treated with cold atmospheric plasma (CAP). Major results for this work include the following: (i) >5 log...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wang S,Doona CJ,Setlow P,Li YQ

    更新日期:2016-09-16 00:00:00

  • Hydrogen profiles and localization of methanogenic activities in the highly compartmentalized hindgut of soil-feeding higher termites (Cubitermes spp.).

    abstract::It has been shown that the coexistence of methanogenesis and reductive acetogenesis in the hindgut of the wood-feeding termite Reticulitermes flavipes is based largely on the radial distribution of the respective microbial populations and relatively high hydrogen partial pressures in the gut lumen. Using Clark-type mi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Schmitt-Wagner D,Brune A

    更新日期:1999-10-01 00:00:00

  • TetR-based gene regulation systems for Francisella tularensis.

    abstract::There are a number of genetic tools available for studying Francisella tularensis, the etiological agent of tularemia; however, there is no effective inducible or repressible gene expression system. Here, we describe inducible and repressible gene expression systems for F. tularensis based on the Tet repressor, TetR. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: LoVullo ED,Miller CN,Pavelka MS Jr,Kawula TH

    更新日期:2012-10-01 00:00:00

  • Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.

    abstract::A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Delécluse A,Rosso ML,Ragni A

    更新日期:1995-12-01 00:00:00

  • Oxidation of chloroform in an aerobic soil exposed to natural gas.

    abstract::Acclimation of a sandy soil to an air-natural gas mixture stimulated the biological oxidation of chloroform to carbon dioxide. Acetylene and methane inhibited chloroform oxidation. Chloroform oxidation continued up to 31 days in the absence of methane. Chloroform oxidation rates increased at chloroform concentrations ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Strand SE,Shippert L

    更新日期:1986-07-01 00:00:00

  • Inhibition of Babesia spp. by plastics.

    abstract::Five commercially available plastic containers were compared with glass for toxicity toward Babesia rodhaini and Babesia bigemina. Comparisons were made by using infectivity tests in mice (B. rodhaini) and cattle (B. bigemina). Low-density polypropylene and polystyrene containers were not toxic, but two of the three p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Timms P

    更新日期:1981-01-01 00:00:00

  • A novel chromogenic ester agar medium for detection of Salmonellae.

    abstract::A novel agar medium, chromogenic Salmonella esterase (CSE) agar, for the differentiation of salmonellae is described. The agar contains peptones and nutrient extracts together with the following (grams per liter unless otherwise specified): 4-[2-(4-octanoyloxy-3, 5-dimethoxyphenyl)-vinyl]-quinolinium-1-(propan-3-yl ca...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Cooke VM,Miles RJ,Price RG,Richardson AC

    更新日期:1999-02-01 00:00:00

  • Enumeration of polysaccharide-degrading Bacteroides species in human feces by using species-specific DNA probes.

    abstract::DNA probes that are specific for each of five predominant species of human colonic Bacteroides (B. thetaiotaomicron, B. uniformis, B. distasonis, "Bacteroides group 3452-A", and B. ovatus) were used to detect and enumerate these species in fecal samples from two adult volunteers. These five species are capable of ferm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kuritza AP,Shaughnessy P,Salyers AA

    更新日期:1986-02-01 00:00:00

  • Medium for isolation and differentiation of Vibrio parahaemolyticus and Vibrio alginolyticus.

    abstract::Trypticase soy agar supplemented with sucrose, sodium chloride, bile salts, and triphenyltetrazolium chloride is an improved plating medium for the isolation of Vibrio parahaemolyticus from samples of seawater, permitting better differentiation of this organism from Vibrio alginolyticus and other bacteria. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kourany M

    更新日期:1983-01-01 00:00:00

  • Novel polyketide synthase from Nectria haematococca.

    abstract::We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: beta-ketoacyl synthase, acyltransferase...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Graziani S,Vasnier C,Daboussi MJ

    更新日期:2004-05-01 00:00:00

  • Pathogenicity of Listeria monocytogenes grown on crabmeat.

    abstract::The pathogenicity of Listeria monocytogenes as influenced by growth on crabmeat at 5 and 10 degrees C was studied. Crabmeat was inoculated with L. monocytogenes V7 (ca. 10(4) CFU/g) and incubated for up to 14 days at 5 and 10 degrees C. At selected incubation times, L. monocytogenes was removed from crabmeat by washin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Brackett RE,Beuchat LR

    更新日期:1990-05-01 00:00:00

  • Selective enrichment with a resuscitation step for isolation of freeze-injured Escherichia coli O157:H7 from foods.

    abstract::We studied injury of Escherichia coli O157:H7 cells in 11 food items during freeze storage and methods of isolating freeze-injured E. coli O157:H7 cells from foods. Food samples inoculated with E. coli O157:H7 were stored for 16 weeks at -20 degrees C in a freezer. Noninjured and injured cells were counted by using tr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hara-Kudo Y,Ikedo M,Kodaka H,Nakagawa H,Goto K,Masuda T,Konuma H,Kojima T,Kumagai S

    更新日期:2000-07-01 00:00:00

  • Lipase expression in Pseudomonas alcaligenes is under the control of a two-component regulatory system.

    abstract::Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. In an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Krzeslak J,Gerritse G,van Merkerk R,Cool RH,Quax WJ

    更新日期:2008-03-01 00:00:00