Contribution of surfactin and SwrA to flagellin expression, swimming, and surface motility in Bacillus subtilis.

abstract：:The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies...

journal_title：Applied and environmental microbiology

authors： Booijink CC,Boekhorst J,Zoetendal EG,Smidt H,Kleerebezem M,de Vos WM

更新日期：2010-08-01 00:00:00

abstract：:Microwave radiation in Escherichia coli and Bacillus subtilis cell suspensions resulted in a dramatic reduction of the viable counts as well as increases in the amounts of DNA and protein released from the cells according to the increase of the final temperature of the cell suspensions. However, no significant reducti...

journal_title：Applied and environmental microbiology

authors： Woo IS,Rhee IK,Park HD

更新日期：2000-05-01 00:00:00

abstract：:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

journal_title：Applied and environmental microbiology

authors： Memmott SD,Ha YS,Dickman MB

更新日期：2002-04-01 00:00:00

abstract：:To understand how microbial communities and functional genes respond to arsenic contamination in the rhizosphere of Pteris vittata, five soil samples with different arsenic contamination levels were collected from the rhizosphere of P. vittata and nonrhizosphere areas and investigated by Biolog, geochemical, and funct...

journal_title：Applied and environmental microbiology

authors： Xiong J,Wu L,Tu S,Van Nostrand JD,He Z,Zhou J,Wang G

更新日期：2010-11-01 00:00:00

abstract：:Downstream from the ptsHI operon of Lactobacillus sakei, the genes atkY and atkB, organized in an operon, were observed. The two putative proteins, AtkB and AtkY, show sequence similarity to the Enterococcus hirae copper P-type ATPase, responsible for copper efflux, and its negative regulator. Characterization of AtkB...

journal_title：Applied and environmental microbiology

authors： Stentz R,Loizel C,Malleret C,Zagorec M

更新日期：2000-10-01 00:00:00

abstract：:The culturability of Vibrio cholerae O1 serotype Inaba strain 569B was decreased by the addition of glucose to cell suspensions in starvation media. A similar effect was observed with sucrose, maltose, and fructose. We term this inhibitory effect glucose shock. It was not observed with arabinose or xylose or with carb...

journal_title：Applied and environmental microbiology

authors： Shiba T,Hill RT,Straube WL,Colwell RR

更新日期：1995-07-01 00:00:00

abstract：:We used an inverse metabolic engineering approach to identify gene targets for improved xylose assimilation in recombinant Saccharomyces cerevisiae. Specifically, we created a genomic fragment library from Pichia stipitis and introduced it into recombinant S. cerevisiae expressing XYL1 and XYL2. Through serial subcult...

journal_title：Applied and environmental microbiology

authors： Jin YS,Alper H,Yang YT,Stephanopoulos G

更新日期：2005-12-01 00:00:00

abstract：:Campylobacter infections are increasing and pose a serious public health problem in Denmark. Infections in humans and broiler flocks show similar seasonality, suggesting that climate may play a role in infection. We examined the effects of temperature, precipitation, relative humidity, and hours of sunlight on Campylo...

journal_title：Applied and environmental microbiology

authors： Patrick ME,Christiansen LE,Wainø M,Ethelberg S,Madsen H,Wegener HC

更新日期：2004-12-01 00:00:00

abstract：:With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless bet...

journal_title：Applied and environmental microbiology

authors： Binishofer B,Moll I,Henrich B,Bläsi U

更新日期：2002-08-01 00:00:00

abstract：:The alphaproteobacterium Magnetospirillum gryphiswaldense has the intriguing ability to navigate within magnetic fields, a behavior named magnetotaxis, governed by the formation of magnetosomes, intracellular membrane-enveloped crystals of magnetite. Magnetosomes are aligned in chains along the cell's motility axis by...

journal_title：Applied and environmental microbiology

authors： Pfeiffer D,Schüler D

更新日期：2020-01-21 00:00:00

abstract：:A new method for the extraction of bacterial DNA from soil has been developed. Soil samples of 50 g were dispersed, and bacteria were released by use of a cation-exchange resin; subsequently, bacteria were separated from soil particles by low-speed centrifugation and lysed with lysozyme and ionic detergent, and the DN...

journal_title：Applied and environmental microbiology

authors： Jacobsen CS,Rasmussen OF

更新日期：1992-08-01 00:00:00

abstract：:We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures between 33 and 39°C. W...

journal_title：Applied and environmental microbiology

authors： Pankowski JA,Puckett SM,Nano FE

更新日期：2016-01-15 00:00:00

abstract：:Three cutinase gene-like genes from the basidiomycete Coprinopsis cinerea (Coprinus cinereus) found with a similarity search were cloned and expressed in Trichoderma reesei under the control of an inducible cbh1 promoter. The selected transformants of all three polyesterase constructs showed activity with p-nitropheny...

journal_title：Applied and environmental microbiology

authors： Kontkanen H,Westerholm-Parvinen A,Saloheimo M,Bailey M,Rättö M,Mattila I,Mohsina M,Kalkkinen N,Nakari-Setälä T,Buchert J

更新日期：2009-04-01 00:00:00

abstract：:We report a method which combines epifluorescence microscopy and microautoradiography to determine both the total number of microorganisms in natural water populations and those individual organisms active in the uptake of specific substrates. After incubation with H-labeled substrate, the sample is filtered and, whil...

journal_title：Applied and environmental microbiology

authors： Tabor PS,Neihof RA

更新日期：1982-10-01 00:00:00

abstract：:The bacterial epiphyte Pseudomonas syringae MF714R was cultured on agar or in broth or collected from colonized leaves; it was then inoculated onto greenhouse-grown bean plants incubated in a growth chamber at low relative humidity or in the field or onto field-grown bean plants. Cells cultured in liquid medium surviv...

journal_title：Applied and environmental microbiology

authors： Wilson M,Lindow SE

更新日期：1993-02-01 00:00:00

abstract：:In various sediments in which Fe(III) reduction was the terminal electron-accepting process, [C]glucose was fermented to C-fatty acids in a manner similar to that observed in methanogenic sediments. These results are consistent with the hypothesis that, in Fe(III)-reducing sediments, fermentable substrates are oxidize...

journal_title：Applied and environmental microbiology

authors： Lovley DR,Phillips EJ

更新日期：1989-12-01 00:00:00

abstract：:The initial microbial colonization of tooth surfaces is a repeatable and selective process, with certain bacterial species predominating in the nascent biofilm. Characterization of the initial microflora is the first step in understanding interactions among community members that shape ensuing biofilm development. Usi...

journal_title：Applied and environmental microbiology

authors： Diaz PI,Chalmers NI,Rickard AH,Kong C,Milburn CL,Palmer RJ Jr,Kolenbrander PE

更新日期：2006-04-01 00:00:00

abstract：:The secondary metabolite mediating the GacS-dependent growth-inhibitory effect exerted by the rice rhizosphere isolate Pseudomonas putida RW10S2 on phytopathogenic Xanthomonas species was identified as white-line-inducing principle (WLIP), a member of the viscosin group of cyclic lipononadepsipeptides. WLIP producers ...

journal_title：Applied and environmental microbiology

authors： Rokni-Zadeh H,Li W,Sanchez-Rodriguez A,Sinnaeve D,Rozenski J,Martins JC,De Mot R

更新日期：2012-07-01 00:00:00

abstract：:A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantificatio...

journal_title：Applied and environmental microbiology

authors： Sails AD,Fox AJ,Bolton FJ,Wareing DR,Greenway DL

更新日期：2003-03-01 00:00:00

abstract：:The alpha-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the LacI/GalR family (galR) of Lactococcus raffinolactis ATCC 43920 were cloned and sequenced. When transferred into Lactococcus lactis and Pediococcus acidilactici strains, aga modified the sugar fermentation profile of...

journal_title：Applied and environmental microbiology

authors： Boucher I,Parrot M,Gaudreau H,Champagne CP,Vadeboncoeur C,Moineau S

更新日期：2002-12-01 00:00:00

abstract：:In Europe the use of the growth promoter avoparcin is considered to have selected for vancomycin-resistant enterococci (VRE). Sweden ceased using avoparcin in 1986, and only occasional cases of VRE from hospitals have been reported since 1995. Within the framework of a European study, samples from urban raw sewage, tr...

journal_title：Applied and environmental microbiology

authors： Iversen A,Kühn I,Franklin A,Möllby R

更新日期：2002-06-01 00:00:00

abstract：:An inexpensive, simple-to-construct nephelometer which was used to monitor the lysis of spheroplasts is described. The nephelometer is a flow-through device with a linear response to cell concentration from the lower detection limit to 8 x 10(8) cells per ml. ...

journal_title：Applied and environmental microbiology

authors： Smith TL,Witter LD

更新日期：1982-12-01 00:00:00

abstract：:Solubilization of coal was demonstrated with filtrates (0.45-mum-pore-size filters) obtained from the broth in which Polyporus versicolor had grown. The rate and extent of solubilization were dependent on the age of the fungal cultures, the particle size of the coal, the pH of the filtrates, and the presence of protei...

journal_title：Applied and environmental microbiology

authors： Cohen MS,Bowers WC,Aronson H,Gray ET

更新日期：1987-12-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:Few studies, if any, have addressed the adhesion of enterococci to the intestinal mucosa and their interference with the adhesion of pathogens, although more than 60% of probiotic preparations in the market contain strains of enterococci. The objective of this study was to investigate if Enterococcus faecium 18C23 has...

journal_title：Applied and environmental microbiology

authors： Jin LZ,Marquardt RR,Zhao X

更新日期：2000-10-01 00:00:00

abstract：:Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

journal_title：Applied and environmental microbiology

authors： McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

更新日期：2001-01-01 00:00:00

abstract：:1,2-Dibromoethane (EDB), which is a groundwater contaminant in areas where it was once used as a soil fumigant, was shown to be degraded aerobically by microorganisms in two types of surface soils from an EDB-contaminated groundwater discharge area. At initial concentrations of 6 to 8 mug/liter, EDB was degraded in a ...

journal_title：Applied and environmental microbiology

authors： Pignatello JJ

更新日期：1986-03-01 00:00:00

abstract：:To further study mechanisms of coping with osmotic stress-low water activity, mutants of Staphylococcus aureus with transposon Tn917-lacZ-induced NaCl sensitivity were selected for impaired ability to grow on solid defined medium containing 2 M NaCl. Southern hybridization experiments showed that NaCl-sensitive mutant...

journal_title：Applied and environmental microbiology

authors： Vijaranakul U,Nadakavukaren MJ,Bayles DO,Wilkinson BJ,Jayaswal RK

更新日期：1997-05-01 00:00:00

abstract：:Shewanella oneidensis MR-1 is a gram-negative facultative anaerobe capable of utilizing a broad range of electron acceptors, including several solid substrates. S. oneidensis MR-1 can reduce Mn(IV) and Fe(III) oxides and can produce current in microbial fuel cells. The mechanisms that are employed by S. oneidensis MR-...

journal_title：Applied and environmental microbiology

authors： Bretschger O,Obraztsova A,Sturm CA,Chang IS,Gorby YA,Reed SB,Culley DE,Reardon CL,Barua S,Romine MF,Zhou J,Beliaev AS,Bouhenni R,Saffarini D,Mansfeld F,Kim BH,Fredrickson JK,Nealson KH

更新日期：2007-11-01 00:00:00

abstract：:The growth of and production of protease, alpha-amylase, alpha-galactosidase, and lipase by Actinomucor taiwanensis in relation to temperature and relative humidity during the preparation of sufu (Chinese cheese) pehtze were investigated. The incubation temperature, humidity, and cultivation time greatly affected the ...

journal_title：Applied and environmental microbiology

authors： Chou CC,Ho FM,Tsai CS

更新日期：1988-03-01 00:00:00