Lon protease influences antibiotic production and UV tolerance of Pseudomonas fluorescens Pf-5.

Abstract:

:Pseudomonas fluorescens Pf-5 is a soil bacterium that suppresses plant pathogens due in part to its production of the antibiotic pyoluteorin. Previous characterization of Pf-5 revealed three global regulators, including the stationary-phase sigma factor sigma(S) and the two-component regulators GacA and GacS, that influence both antibiotic production and stress response. In this report, we describe the serine protease Lon as a fourth global regulator influencing these phenotypes in Pf-5. lon mutants overproduced pyoluteorin, transcribed pyoluteorin biosynthesis genes at enhanced levels, and were more sensitive to UV exposure than Pf-5. The lon gene was preceded by sequences that resembled promoters recognized by the heat shock sigma factor sigma(32) (sigma(H)) of Escherichia coli, and Lon accumulation by Pf-5 increased after heat shock. Therefore, sigma(H) represents the third sigma factor (with sigma(S) and sigma(70)) implicated in the regulation of antibiotic production by P. fluorescens. Lon protein levels were similar in stationary-phase and exponentially growing cultures of Pf-5 and were not positively affected by the global regulator sigma(S) or GacS. The association of antibiotic production and stress response has practical implications for the success of disease suppression in the soil environment, where biological control organisms such as Pf-5 are likely to encounter environmental stresses.

journal_name

Appl Environ Microbiol

authors

Whistler CA,Stockwell VO,Loper JE

doi

10.1128/aem.66.7.2718-2725.2000

keywords:

subject

Has Abstract

pub_date

2000-07-01 00:00:00

pages

2718-25

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

66

pub_type

杂志文章
  • Stable carbon isotope analysis of nucleic acids to trace sources of dissolved substrates used by estuarine bacteria.

    abstract::The natural abundance of stable carbon isotopes measured in bacterial nucleic acids extracted from estuarine bacterial concentrates was used to trace sources of organic matter for bacteria in aquatic environments. The stable carbon isotope ratios of Pseudomonas aeruginosa and nucleic acids extracted from cultures rese...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.7.2012-2020.1990

    authors: Coffin RB,Velinsky DJ,Devereux R,Price WA,Cifuentes LA

    更新日期:1990-07-01 00:00:00

  • Listeria monocytogenes {sigma}B has a small core regulon and a conserved role in virulence but makes differential contributions to stress tolerance across a diverse collection of strains.

    abstract::Listeria monocytogenes strains are classified in at least three distinct phylogenetic lineages. There are correlations between lineage classification and source of bacterial isolation; e.g., human clinical and food isolates usually are classified in either lineage I or II. However, human clinical isolates are overrepr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00031-10

    authors: Oliver HF,Orsi RH,Wiedmann M,Boor KJ

    更新日期:2010-07-01 00:00:00

  • Preferential binding of radiolabeled zearalenone to a protein fraction of Fusarium roseum graminearum.

    abstract::Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.1.80-84.1979

    authors: Inaba T,Mirocha CJ

    更新日期:1979-01-01 00:00:00

  • Key role of cysteine residues in catalysis and subcellular localization of sulfur oxygenase-reductase of Acidianus tengchongensis.

    abstract::Analysis of known sulfur oxygenase-reductases (SORs) and the SOR-like sequences identified from public databases indicated that they all possess three cysteine residues within two conserved motifs (V-G-P-K-V-C(31) and C(101)-X-X-C(104); numbering according to the Acidianus tengchongensis numbering system). The thio-mo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.2.621-628.2005

    authors: Chen ZW,Jiang CY,She Q,Liu SJ,Zhou PJ

    更新日期:2005-02-01 00:00:00

  • Molecular epidemiology of Newcastle disease in Mexico and the potential spillover of viruses from poultry into wild bird species.

    abstract::Newcastle disease, one of the most important health problems that affects the poultry industry around the world, is caused by virulent strains of Newcastle disease virus. Newcastle disease virus is considered to be endemic in several countries in the Americas, including Mexico. In order to control Newcastle disease ou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00993-13

    authors: Cardenas Garcia S,Navarro Lopez R,Morales R,Olvera MA,Marquez MA,Merino R,Miller PJ,Afonso CL

    更新日期:2013-08-01 00:00:00

  • Killing of Staphylococcus aureus via Magnetic Hyperthermia Mediated by Magnetotactic Bacteria.

    abstract::Staphylococcus aureus is a common hospital and household pathogen. Given the emergence of antibiotic-resistant derivatives of this pathogen resulting from the use of antibiotics as general treatment, development of alternative therapeutic strategies is urgently needed. Here, we assess the feasibility of killing S. aur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04103-15

    authors: Chen C,Chen L,Yi Y,Chen C,Wu LF,Song T

    更新日期:2016-02-12 00:00:00

  • Bacterial and Fungal Numbers in Ruminal and Cecal Contents of the Blue Duiker (Cephalophus monticola).

    abstract::[This corrects the article on p. 469 in vol. 57.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.5.1579-1579.1991

    authors:

    更新日期:1991-05-01 00:00:00

  • Soil Fungicides for Controlling Chytridiaceous Mycoparasites of Gigaspora margarita and Glomus fasciculatum.

    abstract::The percentage of azygospores of Gigaspora margarita with zoosporangia of chytridiaceous fungi (CF) was reduced significantly after agitating them in fenaminosulf before incubation in soil. Fenaminosulf did not affect zoosporangia development on chlamydospores of Glomus fasciculatum. Metalaxyl and ethazol were not eff...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.4.1306-1309.1983

    authors: Sylvia DM,Schenck NC

    更新日期:1983-04-01 00:00:00

  • Kinetics, stereospecificity, and expression of the malolactic enzyme.

    abstract::Mass spectrometric measurement of carbon dioxide production was used to study malolactic fermentation (MLF) in Lactobacillus collinoides isolated from cider. The kinetics and stereospecificity of the malolactic enzyme (MLE) were studied, and the stoichiometry of the reaction sequence was investigated. The optimum pH f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.8.3360-3363.1999

    authors: Arthurs CE,Lloyd D

    更新日期:1999-08-01 00:00:00

  • Multiplex PCR method for detection of three Aeromonas enterotoxin genes.

    abstract::A novel multiplex PCR method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in Aeromonas spp. This assay was used to characterize 35 reference strains as well as 537 food-borne isolates. A total of seven gene patte...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01357-09

    authors: Kingombe CI,D'Aoust JY,Huys G,Hofmann L,Rao M,Kwan J

    更新日期:2010-01-01 00:00:00

  • Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium.

    abstract::Based on results from eight experiments, no overall difference was found between roll tube and three- and five-tube most-probable-number (MPN) methods for estimating total numbers of ruminal bacteria. However, standard errors for the replicate means within an experiment were higher with the MPN procedures. Visual grow...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.11.2789-2792.1989

    authors: Dehority BA,Tirabasso PA,Grifo AP Jr

    更新日期:1989-11-01 00:00:00

  • Ammonia production by ruminal microorganisms and enumeration, isolation, and characterization of bacteria capable of growth on peptides and amino acids from the sheep rumen.

    abstract::Excessive NH(3) production in the rumen is a major nutritional inefficiency in ruminant animals. Experiments were undertaken to compare the rates of NH(3) production from different substrates in ruminal fluid in vitro and to assess the role of asaccharolytic bacteria in NH(3) production. Ruminal fluid was taken from f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.10.4925-4931.2002

    authors: Eschenlauer SC,McKain N,Walker ND,McEwan NR,Newbold CJ,Wallace RJ

    更新日期:2002-10-01 00:00:00

  • Fluorene Oxidation In Vivo by Phanerochaete chrysosporium and In Vitro during Manganese Peroxidase-Dependent Lipid Peroxidation.

    abstract::The oxidation of fluorene, a polycyclic hydrocarbon which is not a substrate for fungal lignin peroxidase, was studied in liquid cultures of Phanerochaete chrysosporium and in vitro with P. chrysosporium extracellular enzymes. Intact fungal cultures metabolized fluorene to 9-hydroxyfluorene via 9-fluorenone. Some conv...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.5.1788-1792.1996

    authors: Bogan BW,Lamar RT,Hammel KE

    更新日期:1996-05-01 00:00:00

  • Fundamental procedures for determining ergosterol content of decaying plant material by liquid chromatography.

    abstract::Portions of published procedures for measurement of ergosterol content of decomposing plants were examined for their influence upon ergosterol yield. Common methods of treatment of plant samples prior to sterol extraction (e.g., oven drying, freezing, lyophilization) led to reduced recoveries of ergosterol (ca. 20 to ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.7.1876-1879.1988

    authors: Newell SY,Arsuffi TL,Fallon RD

    更新日期:1988-07-01 00:00:00

  • Application of halophilic nuclease H of Micrococcus varians subsp. halophilus to commercial production of flavoring agent 5'-GMP.

    abstract::RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.4.994-995.1982

    authors: Kamekura M,Hamakawa T,Onishi H

    更新日期:1982-10-01 00:00:00

  • Competition of Rhizobium japonicum Strains in Early Stages of Soybean Nodulation.

    abstract::The effects of preexposure of soybean (Glycine max L. Merrill) roots to Rhizobium japonicum strains and subsequent establishment of other strains in the nodules were investigated by using combinations of effective strains (USDA 110 and USDA 138) and effective-ineffective strains (USDA 110 and SM-5). Strain USDA 110 wa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.4.870-873.1983

    authors: Kosslak RM,Bohlool BB,Dowdle S,Sadowsky MJ

    更新日期:1983-10-01 00:00:00

  • A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture.

    abstract::A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantificatio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1383-1390.2003

    authors: Sails AD,Fox AJ,Bolton FJ,Wareing DR,Greenway DL

    更新日期:2003-03-01 00:00:00

  • Effect of raw-milk cheese consumption on the enterococcal flora of human feces.

    abstract::Enterococci are one of the major facultative anaerobic bacterial groups that reside in the human gastrointestinal tract. In the present study, the composition of the enterococcal fecal flora in three healthy humans was analyzed before, during, and after the daily consumption of approximately 125 g of a raw-milk Chedda...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.1.312-319.2003

    authors: Gelsomino R,Vancanneyt M,Cogan TM,Swings J

    更新日期:2003-01-01 00:00:00

  • Antagonism of Lactic Acid Bacteria against Phytopathogenic Bacteria.

    abstract::A variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens Xanthomonas campestris, Erwinia carotovora, and Pseudomonas syringae. Effective "in vitro" inhibition was found both on agar plates and in broth cultures. I...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.3.552-555.1986

    authors: Visser R,Holzapfel WH,Bezuidenhout JJ,Kotzé JM

    更新日期:1986-09-01 00:00:00

  • Tailing of thermal inactivation curve of Aspergillus niger spores.

    abstract::The nonlinear thermal inactivation of Aspergillus niger spores in phosphate-citrate buffer was studied. The thermal inactivation pattern of the spore consisted of a shoulder, an accelerated decline, and a tail at various constant temperatures around 60 degrees C. The pattern fitted a thermotolerant subpopulation model...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.10.3745-3749.1996

    authors: Fujikawa H,Itoh T

    更新日期:1996-10-01 00:00:00

  • Gene expression induced in Escherichia coli O157:H7 upon exposure to model apple juice.

    abstract::Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription pattern...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02841-08

    authors: Bergholz TM,Vanaja SK,Whittam TS

    更新日期:2009-06-01 00:00:00

  • New Selective Growth Inhibitors of Sphaerotilus natans, Anslimins A and B.

    abstract::Selective growth inhibitors of Sphaerotilus natans were detected in the culture broths of several strains of Streptomyces. Anslimins A and B were isolated from the culture broth of Streptomyces oganonensis by adsorption on resinous adsorbent followed by elution with 50% aqueous acetone. The A and B components were the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.6.1123-1128.1980

    authors: Takiguchi Y,Yoshikawa H,Terao M

    更新日期:1980-06-01 00:00:00

  • Binding of Cyt1Aa and Cry11Aa toxins of Bacillus thuringiensis serovar israelensis to brush border membrane vesicles of Tipula paludosa (Diptera: Nematocera) and subsequent pore formation.

    abstract::Bacillus thuringiensis serovar israelensis (B. thuringiensis subsp. israelensis) produces four insecticidal crystal proteins (ICPs) (Cry4A, Cry4B, Cry11A, and Cyt1A). Toxicity of recombinant B. thuringiensis subsp. israelensis strains expressing only one of the toxins was determined with first instars of Tipula paludo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01056-06

    authors: Oestergaard J,Ehlers RU,Martínez-Ramírez AC,Real MD

    更新日期:2007-06-01 00:00:00

  • Host Ranges of the IncN Group Plasmid pCU1 and Its Minireplicon in Gram-Negative Purple Bacteria.

    abstract::The bacterial host ranges of the conjugatively self-transmissible IncN group plasmid pCU1 and its mobilizable miniderivative, pCU785, were examined. Species of the gram-negative purple bacteria were chosen for this study. Conjugative mobilization of pCU785 into a wide variety of bacteria was facilitated by the presenc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.9.2273-2276.1988

    authors: Krishnan BR,Iyer VN

    更新日期:1988-09-01 00:00:00

  • Differential carbohydrate media and anaerobic replica plating techniques in delineating carbohydrate-utilizing subgroups in rumen bacterial populations.

    abstract::A basal (BC) medium devoid of added carbohydrates, a complete (CC) medium containing nine carbohydrates were developed for enumerating rumen bacteria. The colony counts on the BC medium were 85 to 100% of those obtained on the CC medium. These colonies were pinpoint size (less than or equal to mm in diameter) but incr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.4.709-719.1980

    authors: Leedle JA,Hespell RB

    更新日期:1980-04-01 00:00:00

  • Use of a species-specific DNA hybridization probe for enumerating Bacteroides vulgatus in human feces.

    abstract::pBV-1, a recombinant plasmid that contains a chromosomal DNA fragment from Bacteroides vulgatus, hybridized to DNA from B. vulgatus but not to DNA from other colonic Bacteroides species. This plasmid was used as a DNA probe to detect and enumerate B. vulgatus in pure culture, in mixed cultures, and in a bacterial frac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.4.958-964.1985

    authors: Kuritza AP,Salyers AA

    更新日期:1985-10-01 00:00:00

  • Genetic evidence that high noninduced maltase and maltose permease activities, governed by MALx3-encoded transcriptional regulators, determine efficiency of gas production by baker's yeast in unsugared dough.

    abstract::Strain selection and improvement in the baker's yeast industry have aimed to increase the speed of maltose fermentation in order to increase the leavening activity of industrial baking yeast. We identified two groups of baker's strains of Saccharomyces cerevisiae that can be distinguished by the mode of regulation of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.2.680-685.1999

    authors: Higgins VJ,Braidwood M,Bell P,Bissinger P,Dawes IW,Attfield PV

    更新日期:1999-02-01 00:00:00

  • New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon.

    abstract::We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mappin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.1.278-282.1999

    authors: Katzen F,Becker A,Ielmini MV,Oddo CG,Ielpi L

    更新日期:1999-01-01 00:00:00

  • The MAP kinase Slt2 is involved in vacuolar function and actin remodeling in Saccharomyces cerevisiae mutants affected by endogenous oxidative stress.

    abstract::Oxidative stress causes transient actin cytoskeleton depolarization and also provokes vacuole fragmentation in wild-type cells. Under conditions of oxidative stress induced by hydrogen peroxide, the Slt2 protein is required to repolarize the actin cytoskeleton and to promote vacuole fusion. In this study, we show that...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01692-13

    authors: Pujol-Carrion N,Petkova MI,Serrano L,de la Torre-Ruiz MA

    更新日期:2013-10-01 00:00:00

  • Antimicrobial Peptide GH12 Prevents Dental Caries by Regulating Dental Plaque Microbiota.

    abstract::Due to the complex microecology and microenvironment of dental plaque, novel caries prevention strategies require modulating the microbial communities ecologically and reducing the cariogenic properties effectively. Antimicrobial peptide GH12 reduced the lactic acid production and exopolysaccharide (EPS) synthesis of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00527-20

    authors: Jiang W,Wang Y,Luo J,Chen X,Zeng Y,Li X,Feng Z,Zhang L

    更新日期:2020-07-02 00:00:00