Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.

abstract：:The effects of sublethal concentrations of a variety of chemicals on the susceptibility of rainbow trout (Oncorhynchus mykiss) juveniles to Saprolegnia parasitica infection was examined. Sublethal concentrations of un-ionized ammonia (0.05 mg/liter) and nitrite (0.12 mg/liter) increased fish susceptibility after 10 da...

journal_title：Applied and environmental microbiology

authors： Carballo M,Muñoz MJ

更新日期：1991-06-01 00:00:00

abstract：:Plasmid constructs pNW1 through pNW6 containing a controllable xylE gene (for catechol 2,3-dioxygenase) were introduced into Streptomyces lividans strains to provide a selectable marker system. xylE functions in S. lividans under the control of bacteriophage lambda promoters lambda pL and lambda pR. Thermoregulated ex...

journal_title：Applied and environmental microbiology

authors： Wipat A,Wellington EM,Saunders VA

更新日期：1991-11-01 00:00:00

abstract：:Fresh produce is known to carry nonpathogenic epiphytic microorganisms. During agricultural production and harvesting, leafy greens can become contaminated with antibiotic-resistant pathogens or commensals from animal and human sources. As lettuce does not undergo any inactivation or preservation treatment during proc...

journal_title：Applied and environmental microbiology

authors： Holvoet K,Sampers I,Callens B,Dewulf J,Uyttendaele M

更新日期：2013-11-01 00:00:00

abstract：:Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition as...

journal_title：Applied and environmental microbiology

authors： Hernández-Rodríguez CS,Hernández-Martínez P,Van Rie J,Escriche B,Ferré J

更新日期：2012-06-01 00:00:00

abstract：:While the contribution of Bacteria to bioremediation of oil-contaminated shorelines is well established, the response of Archaea to spilled oil and bioremediation treatments is unknown. The relationship between archaeal community structure and oil spill bioremediation was examined in laboratory microcosms and in a bio...

journal_title：Applied and environmental microbiology

authors： Röling WF,de Brito Couto IR,Swannell RP,Head IM

更新日期：2004-05-01 00:00:00

abstract：:The arginine deiminase system was found to function in protecting bacterial cells against the damaging effects of acid environments. For example, as little as 2.9 mM arginine added to acidified suspensions of Streptococcus sanguis at a pH of 4.0 resulted in ammonia production and protection against killing. The argini...

journal_title：Applied and environmental microbiology

authors： Casiano-Colón A,Marquis RE

更新日期：1988-06-01 00:00:00

abstract：:Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...

journal_title：Applied and environmental microbiology

authors： Uchiyama H,Nakajima T,Yagi O,Nakahara T

更新日期：1992-09-01 00:00:00

abstract：:We investigated the genetic diversity of symbiotic bacteria associated with two newly discovered species of Osedax from Monterey Canyon, CA, at 1,017-m (Osedax Monterey Bay sp. 3 "rosy" [Osedax sp. MB3]) and 381-m (Osedax Monterey Bay sp. 4 "yellow collar") depths. Quantitative PCR and clone libraries of 16S rRNA gene...

journal_title：Applied and environmental microbiology

authors： Goffredi SK,Johnson SB,Vrijenhoek RC

更新日期：2007-04-01 00:00:00

abstract：:The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...

journal_title：Applied and environmental microbiology

authors： Halos L,Jamal T,Maillard R,Girard B,Guillot J,Chomel B,Vayssier-Taussat M,Boulouis HJ

更新日期：2004-10-01 00:00:00

abstract：:Stachybotrys atra is the etiologic agent of stachybotryotoxicosis, and this fungus and its trichothecene mycotoxins were recently implicated in an outbreak of unexplained illness in homes. S. atra was grown on sterile rice, autoclaved, dried, and then aerosolized by acoustic vibration. The distribution of particles (m...

journal_title：Applied and environmental microbiology

authors： Sorenson WG,Frazer DG,Jarvis BB,Simpson J,Robinson VA

更新日期：1987-06-01 00:00:00

abstract：:A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential...

journal_title：Applied and environmental microbiology

authors： Quéméneur M,Heinrich-Salmeron A,Muller D,Lièvremont D,Jauzein M,Bertin PN,Garrido F,Joulian C

更新日期：2008-07-01 00:00:00

abstract：:Four hitherto undescribed Clostridium strains capable of cleaving the C ring of quercetin, kaempferol, and naringenin at C-3-C-4 were isolated from the fecal flora of humans. None of the strains cleaved catechin. C-ring fission occurred when the substrate was either in solution or in suspension. Mixed cultures of flav...

journal_title：Applied and environmental microbiology

authors： Winter J,Moore LH,Dowell VR Jr,Bokkenheuser VD

更新日期：1989-05-01 00:00:00

abstract：:Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bott...

journal_title：Applied and environmental microbiology

authors： Loy A,Beisker W,Meier H

更新日期：2005-07-01 00:00:00

abstract：:The kinetics of the enzymatic formation of 7,8-epoxy-1-octene, 1,2-7,8-diepoxyoctane, and 1,2-epoxyoctane by growing and resting cell suspensions of Pseudomonas oleovorans are described. Formation of 1,2-epoxyoctane occurs concurrently with exponential growth on 1-octene, providing that 1-octene is in excess. Conversi...

journal_title：Applied and environmental microbiology

authors： Schwartz RD,McCoy CJ

更新日期：1976-01-01 00:00:00

abstract：:Newcastle disease, one of the most important health problems that affects the poultry industry around the world, is caused by virulent strains of Newcastle disease virus. Newcastle disease virus is considered to be endemic in several countries in the Americas, including Mexico. In order to control Newcastle disease ou...

journal_title：Applied and environmental microbiology

authors： Cardenas Garcia S,Navarro Lopez R,Morales R,Olvera MA,Marquez MA,Merino R,Miller PJ,Afonso CL

更新日期：2013-08-01 00:00:00

abstract：:The primary goal of this study was to determine the conditions required for the effective inactivation of Bacillus anthracis spores on materials by using methyl bromide (MeBr) gas. Another objective was to obtain comparative decontamination efficacy data with three avirulent microorganisms to assess their potential fo...

journal_title：Applied and environmental microbiology

authors： Wood JP,Wendling M,Richter W,Lastivka A,Mickelsen L

更新日期：2016-01-22 00:00:00

abstract：:Staphylococcus aureus Cowan 1 cells were armed with anti-flagellar (anti-H) antibody produced in rabbits immunized with flagellar core protein prepared from Vibrio vulnificus. This reagent was assessed by coagglutination for its capacity to agglutinate and identify V. vulnificus. A species-specific H antigen is expres...

journal_title：Applied and environmental microbiology

authors： Simonson J,Siebeling RJ

更新日期：1986-12-01 00:00:00

abstract：:A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility receiving industrial waste containing ferric-EDTA. The isolate grew on ferric-EDTA as the sole carbon source at concentrations exceed...

journal_title：Applied and environmental microbiology

authors： Lauff JJ,Steele DB,Coogan LA,Breitfeller JM

更新日期：1990-11-01 00:00:00

abstract：:The potential for aerobic methyl tert-butyl ether (MTBE) degradation was investigated with microcosms containing aquifer sediment and groundwater from four MTBE-contaminated sites characterized by oxygen-limited in situ conditions. MTBE depletion was observed for sediments from two sites (e.g., 4.5 mg/liter degraded i...

journal_title：Applied and environmental microbiology

authors： Kane SR,Beller HR,Legler TC,Koester CJ,Pinkart HC,Halden RU,Happel AM

更新日期：2001-12-01 00:00:00

abstract：:In this study, a number of Listeria monocytogenes strains of different origins were evaluated for in vitro invasion capacity for various human cell types (monocytic THP-1, enterocytic Caco-2, and hepatocytic HepG2 cells) and for expression levels of specific virulence genes. For THP-1 cells, no differences between cli...

journal_title：Applied and environmental microbiology

authors： Werbrouck H,Grijspeerdt K,Botteldoorn N,Van Pamel E,Rijpens N,Van Damme J,Uyttendaele M,Herman L,Van Coillie E

更新日期：2006-06-01 00:00:00

abstract：:The effects of polychlorinated biphenyl (PCB) and phenanthrene stress on glucose uptake by natural microbial populations were examined by the heterotrophic potential technique. Temporal and spatial distributions in glucose uptake velocities were examined for natural samples as well as PCB- and phenanthrene-stressed sa...

journal_title：Applied and environmental microbiology

authors： Sayler GS,Lund LC,Shiaris MP,Sherrill TW,Perkins RE

更新日期：1979-05-01 00:00:00

abstract：:The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP wit...

journal_title：Applied and environmental microbiology

authors： Jain RK,Dreisbach JH,Spain JC

更新日期：1994-08-01 00:00:00

abstract：:Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ...

journal_title：Applied and environmental microbiology

authors： Asgeirsdóttir SA,Scholtmeijer K,Wessels JG

更新日期：1999-05-01 00:00:00

abstract：:Multicellular communities produced by Bacillus subtilis can adopt sliding or swarming to translocate over surfaces. While sliding is a flagellum-independent motility produced by the expansive forces in a growing colony, swarming requires flagellar functionality and is characterized by the appearance of hyperflagellate...

journal_title：Applied and environmental microbiology

authors： Ghelardi E,Salvetti S,Ceragioli M,Gueye SA,Celandroni F,Senesi S

更新日期：2012-09-01 00:00:00

abstract：:Sakacin A is an antilisterial bacteriocin produced by Lactobacillus sake Lb706. In order to identify genes involved in sakacin A production and immunity, the plasmid fraction of L. sake Lb706 was shotgun cloned directly into a sakacin A-nonproducing and -sensitive variant, L. sake Lb706-B, by using the broad-host-rang...

journal_title：Applied and environmental microbiology

authors： Axelsson L,Holck A,Birkeland SE,Aukrust T,Blom H

更新日期：1993-09-01 00:00:00

abstract：:The biotinylating reagent succinimidyl 6-(biotinamido)hexanoate was used to label the cell surfaces of the cosmopolitan, marine, eukaryotic microorganism Emiliania huxleyi under different growth conditions. Proteins characteristic of different nutrient conditions could be identified. In particular, a nitrogen-regulate...

journal_title：Applied and environmental microbiology

authors： Palenik B,Koke JA

更新日期：1995-09-01 00:00:00

abstract：:A method was developed to detect NO- or N(2)O-producing bacteria in solid or liquid medium by their ability to oxidize the redox indicator resazurin from its reduced colorless form to its oxidized pink form. The method was sensitive to as little as 35 nM N(2)O or 0.5 nM NO. Ninety-one percent of the colonies that oxid...

journal_title：Applied and environmental microbiology

authors： Jenneman GE,Montgomery AD,McInerney MJ

更新日期：1986-04-01 00:00:00

abstract：:Inhibition of enzyme activity by high concentrations of substrate and/or cofactor is a general phenomenon demonstrated in many enzymes, including aldehyde dehydrogenases. Here we show that the uncharacterized protein BetB (SA2613) from Staphylococcus aureus is a highly specific betaine aldehyde dehydrogenase, which ex...

journal_title：Applied and environmental microbiology

authors： Chen C,Joo JC,Brown G,Stolnikova E,Halavaty AS,Savchenko A,Anderson WF,Yakunin AF

更新日期：2014-07-01 00:00:00

abstract：:Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that ...

journal_title：Applied and environmental microbiology

authors： Huber H,Stetter KO

更新日期：1990-02-01 00:00:00

abstract：:Facultative endosymbionts can benefit insect hosts in a variety of ways, including context-dependent roles, such as providing defense against pathogens. The role of some symbionts in defense may be overlooked, however, when pathogen infection is transient, sporadic, or asymptomatic. The facultative endosymbiont Ricket...

journal_title：Applied and environmental microbiology

authors： Hendry TA,Hunter MS,Baltrus DA

更新日期：2014-12-01 00:00:00