Method for detection of microorganisms that produce gaseous nitrogen oxides.

Abstract:

:A method was developed to detect NO- or N(2)O-producing bacteria in solid or liquid medium by their ability to oxidize the redox indicator resazurin from its reduced colorless form to its oxidized pink form. The method was sensitive to as little as 35 nM N(2)O or 0.5 nM NO. Ninety-one percent of the colonies that oxidized resazurin on plates also produced N(2)O in slant cultures. Forty-four percent of the colonies that did not oxidize resazurin did produce N(2)O. This percentage was reduced to 15% when colonies in which the coloration was difficult to discern were picked to slants to determine whether they oxidized the slant. The production of N(2)O preceded the oxidation of resazurin by liquid cultures of Escherichia coli and a sludge isolate. With the denitrifying sewage isolate, the disappearance of N(2)O was followed by the return of resazurin to its reduced state. Wolinella succinogenes was found to produce small amounts of N(2)O from NO(3), which resulted in a transient oxidation of resazurin.

journal_name

Appl Environ Microbiol

authors

Jenneman GE,Montgomery AD,McInerney MJ

doi

10.1128/AEM.51.4.776-780.1986

keywords:

subject

Has Abstract

pub_date

1986-04-01 00:00:00

pages

776-80

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

51

pub_type

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