Abstract:
:Lactobacillus plantarum is an attractive candidate for bioprocessing of lignocellulosic biomass due to its high metabolic variability, including its ability to ferment both pentoses and hexoses, as well as its high acid tolerance, a quality often utilized in industrial processes. This bacterium grows naturally on biomass; however, it lacks the inherent ability to deconstruct lignocellulosic substrates. As a first step toward engineering lignocellulose-converting lactobacilli, we have introduced genes coding for a GH6 cellulase and a GH11 xylanase from a highly active cellulolytic bacterium into L. plantarum. For this purpose, we employed the recently developed pSIP vectors for efficient secretion of heterologous proteins. Both enzymes were secreted by L. plantarum at levels estimated at 0.33 nM and 3.3 nM, for the cellulase and xylanase, respectively, in culture at an optical density at 600 nm (OD600) of 1. Transformed cells demonstrated the ability to degrade individually either cellulose or xylan and wheat straw. When mixed together to form a two-strain cell-based consortium secreting both cellulase and xylanase, they exhibited synergistic activity in the overall release of soluble sugar from wheat straw. This result paves the way toward metabolic harnessing of L. plantarum for novel biorefining applications, such as production of ethanol and polylactic acid directly from plant biomass.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Moraïs S,Shterzer N,Grinberg IR,Mathiesen G,Eijsink VG,Axelsson L,Lamed R,Bayer EA,Mizrahi Idoi
10.1128/AEM.01211-13subject
Has Abstractpub_date
2013-09-01 00:00:00pages
5242-9issue
17eissn
0099-2240issn
1098-5336pii
AEM.01211-13journal_volume
79pub_type
杂志文章abstract::Lipophilic compounds of the culture suspension containing Rhodococcus erythropolis DSM43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source. Thirteen main components from a dichloromethane-methanol extract of the R. erythropolis cultures were isolated and characteri...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.44.4.864-870.1982
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.40.3.678-680.1980
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更新日期:1991-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.4.2359-2365.2006
更新日期:2006-04-01 00:00:00
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更新日期:1979-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1983-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.4.794-797.1989
更新日期:1989-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2015-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00