Abstract:
:We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lactone production. Monodisrupted strains produced similar levels of lactone as the wild-type strain (50 mg/liter) except for Deltapox3, which produced 220 mg of gamma-decalactone per liter after 24 h. The Deltapox2 Deltapox3 double-disrupted strain, although slightly affected in growth, produced about 150 mg of lactone per liter, indicating that Aox2p was not essential for the biotransformation. The Deltapox2 Deltapox3 Deltapox5 triple-disrupted strain produced and consumed lactone very slowly. On the contrary, the Deltapox2 Deltapox3 Deltapox4 Deltapox5 multidisrupted strain did not grow or biotransform methyl ricinoleate into gamma-decalactone, demonstrating that Aox4p is essential for the biotransformation.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Waché Y,Laroche C,Bergmark K,Møller-Andersen C,Aguedo M,Le Dall MT,Wang H,Nicaud JM,Belin JMdoi
10.1128/aem.66.3.1233-1236.2000keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
1233-6issue
3eissn
0099-2240issn
1098-5336journal_volume
66pub_type
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