Use of antibody responses against locus of enterocyte effacement (LEE)-encoded antigens to monitor enterohemorrhagic Escherichia coli infections on cattle farms.

abstract：:Fructansucrase enzymes polymerize the fructose moiety of sucrose into levan or inulin fructans, with beta(2-6) and beta(2-1) linkages, respectively. The probiotic bacterium Lactobacillus johnsonii strain NCC 533 possesses a single fructansucrase gene (open reading frame AAS08734) annotated as a putative levansucrase p...

journal_title：Applied and environmental microbiology

authors： Anwar MA,Kralj S,van der Maarel MJ,Dijkhuizen L

更新日期：2008-06-01 00:00:00

abstract：:The susceptibility of the Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection, and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IK...

journal_title：Applied and environmental microbiology

authors： Fernández-Aguilar X,Cabezón O,Granados JE,Frey J,Serrano E,Velarde R,Cano-Manuel FJ,Mentaberre G,Ráez-Bravo A,Fandos P,López-Olvera JR

更新日期：2017-07-17 00:00:00

abstract：:Lysostaphin represents a promising therapeutic agent for the treatment of staphylococcal infections, in particular those of methicillin-resistant Staphylococcus aureus (MRSA). However, conventional expression systems for the enzyme suffer from various limitations, and there remains a need for an efficient and cost-eff...

journal_title：Applied and environmental microbiology

authors： Zhao H,Blazanovic K,Choi Y,Bailey-Kellogg C,Griswold KE

更新日期：2014-05-01 00:00:00

abstract：:The arginine deiminase system was found to function in protecting bacterial cells against the damaging effects of acid environments. For example, as little as 2.9 mM arginine added to acidified suspensions of Streptococcus sanguis at a pH of 4.0 resulted in ammonia production and protection against killing. The argini...

journal_title：Applied and environmental microbiology

authors： Casiano-Colón A,Marquis RE

更新日期：1988-06-01 00:00:00

abstract：:Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blen...

journal_title：Applied and environmental microbiology

authors： Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JA

更新日期：1990-06-01 00:00:00

abstract：:A mixed methanogenic culture fed with glucose was perturbed with butyrate and formate to investigate the role of formate in the acetogenesis of butyrate. A free energy analysis suggests that formate rather than H(2) was the interspecies electron carrier for butyrate conversion into acetate for the culture studied. ...

journal_title：Applied and environmental microbiology

authors： Bae J,McCarty PL

更新日期：1993-02-01 00:00:00

abstract：:Race 1 isolates of Fusarium oxysporum f. sp. lycopersici (FOL) are characterized by the presence of AVR1 in their genomes. The product of this gene, Avr1, triggers resistance in tomato cultivars carrying resistance gene I In FOL race 2 and race 3 isolates, AVR1 is absent, and hence they are virulent on tomato cultivar...

journal_title：Applied and environmental microbiology

authors： Biju VC,Fokkens L,Houterman PM,Rep M,Cornelissen BJC

更新日期：2017-02-01 00:00:00

abstract：:Survival studies were conducted on Legionella pneumophila cells that had been grown intracellularly in Acanthamoeba polyphaga and then exposed to polyhexamethylene biguanide (PHMB), benzisothiazolone (BIT), and 5-chloro-N-methylisothiazolone (CMIT). Susceptibilities were also determined for L. pneumophila grown under ...

journal_title：Applied and environmental microbiology

authors： Barker J,Brown MR,Collier PJ,Farrell I,Gilbert P

更新日期：1992-08-01 00:00:00

abstract：:A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...

journal_title：Applied and environmental microbiology

authors： Vesley D,Lillquist DR,Le CT

更新日期：1985-05-01 00:00:00

abstract：:Single 10-bp primers were used to generate random amplified polymorphic DNA (RAPD) markers from commercial and wild strains of the cultivated mushroom Agaricus bisporus via the polymerase chain reaction. Of 20 primers tested, 19 amplified A. bisporus DNA, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0...

journal_title：Applied and environmental microbiology

authors： Khush RS,Becker E,Wach M

更新日期：1992-09-01 00:00:00

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-bi...

journal_title：Applied and environmental microbiology

authors： Nikitkova AE,Haase EM,Scannapieco FA

更新日期：2013-01-01 00:00:00

abstract：:Strain selection and improvement in the baker's yeast industry have aimed to increase the speed of maltose fermentation in order to increase the leavening activity of industrial baking yeast. We identified two groups of baker's strains of Saccharomyces cerevisiae that can be distinguished by the mode of regulation of ...

journal_title：Applied and environmental microbiology

authors： Higgins VJ,Braidwood M,Bell P,Bissinger P,Dawes IW,Attfield PV

更新日期：1999-02-01 00:00:00

abstract：:The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -inde...

journal_title：Applied and environmental microbiology

authors： Chouaia B,Rossi P,Montagna M,Ricci I,Crotti E,Damiani C,Epis S,Faye I,Sagnon N,Alma A,Favia G,Daffonchio D,Bandi C

更新日期：2010-11-01 00:00:00

abstract：:The anoxygenic phototrophic bacterium Rhodopseudomonas palustris produces methane (CH4) from carbon dioxide (CO2) and hydrogen (H2) from protons (H+) when it expresses a variant form of molybdenum (Mo) nitrogenase that has two amino acid substitutions near its active site. We examined the influence of light energy and...

journal_title：Applied and environmental microbiology

authors： Zheng Y,Harwood CS

更新日期：2019-04-18 00:00:00

abstract：:Biotransformation of 2-chlorophenol by a methanogenic sediment community resulted in the transient accumulation of phenol and benzoate. 3-Chlorobenzoate was a more persistent product of 2-chlorophenol metabolism. The anaerobic biotransformation of phenol to benzoate presumably occurred via para-carboxylation and dehyd...

journal_title：Applied and environmental microbiology

authors： Becker JG,Stahl DA,Rittmann BE

更新日期：1999-11-01 00:00:00

abstract：:A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of gro...

journal_title：Applied and environmental microbiology

authors： Wells JM,Cole RJ,Cutler HC,Spalding DH

更新日期：1981-04-01 00:00:00

abstract：:The influence of substrate and enzyme concentrations on the rate of saccharification of two defined insoluble cellulose substrates, Avicel (FMC Corp., Philadelphia, Pa.) and Solka-Floc (James River Co., Berlin, N.H.), by the cellulase enzyme system of Trichoderma viride was evaluated. In the assays, enzyme concentrati...

journal_title：Applied and environmental microbiology

authors： Liaw ET,Penner MH

更新日期：1990-08-01 00:00:00

abstract：:Anoxic sediments from Rotsee (Switzerland) were analyzed for the presence and diversity of methanogens by using molecular tools and for methanogenic activity by using radiotracer techniques, in addition to the measurement of chemical profiles. After PCR-assisted sequence retrieval of the 16S rRNA genes (16S rDNA) from...

journal_title：Applied and environmental microbiology

authors： Zepp Falz K,Holliger C,Grosskopf R,Liesack W,Nozhevnikova AN,Müller B,Wehrli B,Hahn D

更新日期：1999-06-01 00:00:00

abstract：:A field collected population of Plutella xylostella (SERD4) was selected in the laboratory with Bacillus thuringiensis endotoxins Cry1Ac (Cry1Ac-SEL) and Cry1Ab (Cry1Ab-SEL). Both subpopulations showed similar phenotypes: high resistance to the Cry1A toxins and little cross-resistance to Cry1Ca or Cry1D. A previous an...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Ibiza-Palacios MS,Escriche B,Wright DJ,Crickmore N

更新日期：2005-11-01 00:00:00

abstract：:We isolated thermotolerant Gluconobacter strains that are able to produce 5-keto-d-gluconic acid (5KGA) at 37 degrees C, a temperature at which regular mesophilic 5KGA-producing strains showed much less growth and 5KGA production. The thermotolerant strains produced 2KGA as the major product at both 30 and 37 degrees ...

journal_title：Applied and environmental microbiology

authors： Saichana I,Moonmangmee D,Adachi O,Matsushita K,Toyama H

更新日期：2009-07-01 00:00:00

abstract：:The transposon Tn916 and self-mobilizing plasmid pAM beta 1 were conjugated from Enterococcus faecalis to the ruminal bacterium Streptococcus bovis JB1. Transconjugants were identified by resistance to tetracycline (Tn916) or erythromycin (pAM beta 1) and by Southern hybridization analyses. Transfer frequencies were 7...

journal_title：Applied and environmental microbiology

authors： Hespell RB,Whitehead TR

更新日期：1991-09-01 00:00:00

abstract：:Several selective media were evaluated for the primary isolation and enumeration of Staphylococcus aureus from halogenated indoor swimming pool waters. Standard plate counts of the viable population and total coliform densities were also determined to ascertain their value as indicator systems. All studies were done w...

journal_title：Applied and environmental microbiology

authors： Alico RK,Dragonjac MF

更新日期：1986-04-01 00:00:00

abstract：:The effect of solar radiation and predacious microorganisms on the survival of bacteria of fecal and plant origin was studied. The decline in the numbers of Escherichia coli cells in estuarine water samples was found to be significantly greater in the presence of both naturally occurring microbial predators and solar ...

journal_title：Applied and environmental microbiology

authors： McCambridge J,McMeekin TA

更新日期：1981-05-01 00:00:00

abstract：:An amylosucrase gene was subjected to high-rate segmental random mutagenesis, which was directed toward a segment encoding amino acids that influence the interaction with substrate molecules in subsites -1 to +3. A screen was used to identify enzyme variants with compromised glucan chain elongation. With an average mu...

journal_title：Applied and environmental microbiology

authors： Schneider J,Fricke C,Overwin H,Hofmann B,Hofer B

更新日期：2009-12-01 00:00:00

abstract：:Inactivation (loss of culturability) by sunlight of enterococci and fecal coliforms within sewage effluent diluted in seawater was investigated in field experiments. In most experiments, 500-ml flasks of pure silica were used to confine activated sludge effluent diluted to 2% (vol/vol) in seawater. Inactivation of bac...

journal_title：Applied and environmental microbiology

authors： Davies-Colley RJ,Bell RG,Donnison AM

更新日期：1994-06-01 00:00:00

abstract：:Very little is known about the interaction of bryophytes with bacteria. Therefore, we analyzed bacteria associated with three bryophyte species, Tortula ruralis, Aulacomnium palustre, and Sphagnum rubellum, which represent typical moss species of three nutrient-poor plant communities at the southern Baltic Sea coast i...

journal_title：Applied and environmental microbiology

authors： Opelt K,Berg G

更新日期：2004-11-01 00:00:00

abstract：:Citrus canker, caused by Xanthomonas citri pv. citri, is a serious disease of citrus plants worldwide. Earlier phylogenetic studies using housekeeping genes revealed that X. citri pv. citri is related to many other pathovars, which can be collectively referred as Xanthomonas citri pathovars (XCPs). From the present st...

journal_title：Applied and environmental microbiology

authors： Bansal K,Midha S,Kumar S,Patil PB

更新日期：2017-04-17 00:00:00

abstract：:The metal-reducing bacterium Shewanella oneidensis MR-1 displays remarkable anaerobic respiratory plasticity, which is reflected in the extensive number of electron transport components encoded in its genome. In these studies, several cell components required for the reduction of vanadium(V) were determined. V(V) redu...

journal_title：Applied and environmental microbiology

authors： Myers JM,Antholine WE,Myers CR

更新日期：2004-03-01 00:00:00

abstract：:Genome analysis revealed the existence of a putative transcriptional regulatory system governing CO metabolism in Thermococcus onnurineus NA1, a carboxydotrophic hydrogenogenic archaeon. The regulatory system is composed of CorQ with a 4-vinyl reductase domain and CorR with a DNA-binding domain of the LysR-type transc...

journal_title：Applied and environmental microbiology

authors： Kim MS,Choi AR,Lee SH,Jung HC,Bae SS,Yang TJ,Jeon JH,Lim JK,Youn H,Kim TW,Lee HS,Kang SG

更新日期：2015-03-01 00:00:00