A novel CO-responsive transcriptional regulator and enhanced H2 production by an engineered Thermococcus onnurineus NA1 strain.

abstract：:Mycophenolic acid (MPA) is the active ingredient in the increasingly important immunosuppressive pharmaceuticals CellCept (Roche) and Myfortic (Novartis). Despite the long history of MPA, the molecular basis for its biosynthesis has remained enigmatic. Here we report the discovery of a polyketide synthase (PKS), MpaC,...

journal_title：Applied and environmental microbiology

authors： Regueira TB,Kildegaard KR,Hansen BG,Mortensen UH,Hertweck C,Nielsen J

更新日期：2011-05-01 00:00:00

abstract：:The ability of pure cultures of bacteria isolated from spoiling chicken leg muscle to produce strong off-odors was tested by using sterile leg muscle sections. Changes in the flora during storage and the incidence and identity of organisms capable of producing strong off-odors were noted. ...

journal_title：Applied and environmental microbiology

authors： McMeekin TA

更新日期：1977-06-01 00:00:00

abstract：:Receptors on the cell surfaces of bacterial hosts are essential during the infection cycle of bacteriophages. To date, the phage receptors of the industrial relevant dairy starter bacterium Streptococcus thermophilus remain elusive. Thus, we set out to identify cell surface structures that are involved in host recogni...

journal_title：Applied and environmental microbiology

authors： Szymczak P,Filipe SR,Covas G,Vogensen FK,Neves AR,Janzen T

更新日期：2018-11-15 00:00:00

abstract：:Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium ...

journal_title：Applied and environmental microbiology

authors： Muñoz G,Sotomayor P

更新日期：1990-07-01 00:00:00

abstract：:Species diversity, phylogenetic affiliations, and environmental occurrence patterns of thiosulfate-oxidizing marine bacteria were investigated by using new isolates from serially diluted continental slope and deep-sea abyssal plain sediments collected off the coast of New England and strains cultured previously from G...

journal_title：Applied and environmental microbiology

authors： Teske A,Brinkhoff T,Muyzer G,Moser DP,Rethmeier J,Jannasch HW

更新日期：2000-08-01 00:00:00

abstract：:The function of small secreted proteins (SSPs) in saprotrophic fungi is, for the most part, unknown. The white-rot mushroom Pleurotus ostreatus produces considerable amounts of SSPs at the onset of secondary metabolism, during colony development, and in response to chemical compounds such as 5-hydroxymethylfurfural an...

journal_title：Applied and environmental microbiology

authors： Feldman D,Amedi N,Carmeli S,Yarden O,Hadar Y

更新日期：2019-07-18 00:00:00

abstract：:Continuous centrifugation of large volumes of water from natural southeastern lakes allowed quantitative detection of Legionella pneumophila by direct immunofluorescent staining. Positive samples were injected intraperitoneally into guinea pigs, and the L. pneumophila were isolated and identified by their morphologica...

journal_title：Applied and environmental microbiology

authors： Fliermans CB,Cherry WB,Orrison LH,Thacker L

更新日期：1979-06-01 00:00:00

abstract：:Aerobiological monitoring was conducted in an experimental room to aid in the development of standardized sampling protocols for airborne microorganisms in the indoor environment. The objectives of this research were to evaluate the relative efficiencies of selected sampling methods for the retrieval of airborne funga...

journal_title：Applied and environmental microbiology

authors： Buttner MP,Stetzenbach LD

更新日期：1993-01-01 00:00:00

abstract：:The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA

更新日期：1989-12-01 00:00:00

abstract：:Cultures of the newly isolated bacterial strains AD20, AD25, and AD27, identified as strains of Ancylobacter aquaticus, were capable of growth on 1,2-dichloroethane (DCE) as the sole carbon and energy source. These strains, as well as two other new DCE utilizers, were facultative methylotrophs and were also able to gr...

journal_title：Applied and environmental microbiology

authors： van den Wijngaard AJ,van der Kamp KW,van der Ploeg J,Pries F,Kazemier B,Janssen DB

更新日期：1992-03-01 00:00:00

abstract：:Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipi...

journal_title：Applied and environmental microbiology

authors： Martínez B,Böttiger T,Schneider T,Rodríguez A,Sahl HG,Wiedemann I

更新日期：2008-08-01 00:00:00

abstract：:To gain a predictive understanding of the interspecies interactions within microbial communities that govern community function, the genomic complement of every member population must be determined. Although metagenomic sequencing has enabled the de novo reconstruction of some microbial genomes from environmental comm...

journal_title：Applied and environmental microbiology

authors： Nelson WC,Maezato Y,Wu YW,Romine MF,Lindemann SR

更新日期：2015-10-23 00:00:00

abstract：:A variety of lactic acid bacteria were screened for their ability to produce folate intracellularly and/or extracellularly. Lactococcus lactis, Streptococcus thermophilus, and Leuconostoc spp. all produced folate, while most Lactobacillus spp., with the exception of Lactobacillus plantarum, were not able to produce fo...

journal_title：Applied and environmental microbiology

authors： Sybesma W,Starrenburg M,Tijsseling L,Hoefnagel MH,Hugenholtz J

更新日期：2003-08-01 00:00:00

abstract：:To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which ...

journal_title：Applied and environmental microbiology

authors： Wu H,Gao K,Villafañe VE,Watanabe T,Helbling EW

更新日期：2005-09-01 00:00:00

abstract：:A physiological, unbalanced model is presented that explicitly describes growth of the marine cyanobacterium Trichodesmium sp. at the expense of N(2) (diazotrophy). The model involves the dynamics of intracellular reserves of carbon and nitrogen and allows the uncoupling of the metabolism of these elements. The result...

journal_title：Applied and environmental microbiology

authors： Rabouille S,Staal M,Stal LJ,Soetaert K

更新日期：2006-05-01 00:00:00

abstract：:We examined the harmful side effects on indigenous soil microorganisms of two organic solvents, acetone and dichloromethane, that are normally used for spiking of soil with polycyclic aromatic hydrocarbons for experimental purposes. The solvents were applied in two contamination protocols to either the whole soil samp...

journal_title：Applied and environmental microbiology

authors： Brinch UC,Ekelund F,Jacobsen CS

更新日期：2002-04-01 00:00:00

abstract：:An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of chromagenic reaction products, and a simple permeabilization step to ...

journal_title：Applied and environmental microbiology

authors： Paoni NF,Arroyo RL

更新日期：1984-01-01 00:00:00

abstract：:Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are...

journal_title：Applied and environmental microbiology

authors： McCabe PM,Van Alfen NK

更新日期：1999-12-01 00:00:00

abstract：:A radioisotope method was devised to study bacterial respiratory reduction of arsenate in sediments. The following two arsenic-rich soda lakes in California were chosen for comparison on the basis of their different salinities: Mono Lake (approximately 90 g/liter) and Searles Lake (approximately 340 g/liter). Profiles...

journal_title：Applied and environmental microbiology

authors： Kulp TR,Hoeft SE,Miller LG,Saltikov C,Murphy JN,Han S,Lanoil B,Oremland RS

更新日期：2006-10-01 00:00:00

abstract：:The recalcitrance of xenobiotics may be caused by an absence of transforming enzymes or by their inability to enter microbial cells. A nondestructive method for differentiating between these two possibilities is described. The solid n-alkanes octadecane (C(18)) and hexatriacontane (C(36)) were encapsulated into phosph...

journal_title：Applied and environmental microbiology

authors： Miller RM,Bartha R

更新日期：1989-02-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Se...

journal_title：Applied and environmental microbiology

authors： Yoshimasu MA,Zawistowski J

更新日期：2001-01-01 00:00:00

abstract：:The ability of bifidobacteria to adhere to the intestine of the human host is considered to be important for efficient colonization and achieving probiotic effects. Bifidobacterium bifidum strains DSM20456 and MIMBb75 adhere well to the human intestinal cell lines Caco-2 and HT-29. The surface lipoprotein BopA was pre...

journal_title：Applied and environmental microbiology

authors： Kainulainen V,Reunanen J,Hiippala K,Guglielmetti S,Vesterlund S,Palva A,Satokari R

更新日期：2013-11-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:Recently, the lignin-degrading basidiomycete Pleurotus ostreatus has become a widely used model organism for fungal genomic and transcriptomic analyses. The increasing interest in this species has led to an increasing number of studies analyzing the transcriptional regulation of multigene families that encode extracel...

journal_title：Applied and environmental microbiology

authors： Castanera R,López-Varas L,Pisabarro AG,Ramírez L

更新日期：2015-06-15 00:00:00

abstract：:Soil organic carbon (SOC) plays an important role in regulating global climate change, carbon and nutrient cycling in soils, and soil moisture. Organic matter (OM) additions to soils can affect the rate at which SOC is mineralized by microbes, with potentially important effects on SOC stocks. Understanding how pyrogen...

journal_title：Applied and environmental microbiology

authors： Whitman T,DeCiucies S,Hanley K,Enders A,Woolet J,Lehmann J

更新日期：2021-01-29 00:00:00

abstract：:3'-Phosphoadenosine-5'-phosphatase (PAPase) is required for the removal of toxic 3'-phosphoadenosine-5'-phosphate (PAP) produced during sulfur assimilation in various eukaryotic organisms. This enzyme is a well-known target of lithium and sodium toxicity and has been used for the production of salt-resistant transgeni...

journal_title：Applied and environmental microbiology

authors： Zhang JY,Zou J,Bao Q,Chen WL,Wang L,Yang H,Zhang CC

更新日期：2006-01-01 00:00:00

abstract：:Thirteen strains of Agrobacterium tumefaciens isolated from grapevine tumors in northern China were surveyed. These strains varied in their host range properties, although all were tumorigenic on grapevines. Twelve of these strains belonged to Agrobacterium sp. biotype 3, and 11 strains resulted in the synthesis of th...

journal_title：Applied and environmental microbiology

authors： Ma DQ,Yanofsky MF,Gordon MP,Nester EW

更新日期：1987-06-01 00:00:00

abstract：:By taking advantage of MazF, an ACA codon-specific mRNA interferase, Escherichia coli cells can be converted into a bioreactor producing only a single protein of interest by using an ACA-less mRNA for the protein. In this single-protein production (SPP) system, we engineered MazF by replacing two tryptophan residues i...

journal_title：Applied and environmental microbiology

authors： Vaiphei ST,Mao L,Shimazu T,Park JH,Inouye M

更新日期：2010-09-01 00:00:00

abstract：:We previously reported the isolation of a 21.5-kb genomic DNA fragment from Pseudomonas sp. strain ADP, which contains the atzA gene, encoding the first metabolic step for the degradation of the herbicide atrazine (M. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Mic...

journal_title：Applied and environmental microbiology

authors： Boundy-Mills KL,de Souza ML,Mandelbaum RT,Wackett LP,Sadowsky MJ

更新日期：1997-03-01 00:00:00

abstract：:The role of three key nitrogen regulatory genes, glnB (encoding the P(II) protein), glnZ (encoding the P(z) protein), and glnD (encoding the GlnD protein), in regulation of poly-3-hydroxybutyrate (PHB) biosynthesis by ammonia in Azospirillum brasilense Sp7 was investigated. It was observed that glnB glnZ and glnD muta...

journal_title：Applied and environmental microbiology

authors： Sun J,Van Dommelen A,Van Impe J,Vanderleyden J

更新日期：2002-02-01 00:00:00