Abstract:
:Laboratory column studies were conducted at the Utah Water Research Laboratory, Logan, Utah, to evaluate reovirus removal from drinking water supplies by slow-rate sand filtration (SSF). Columns, constructed to simulate a full-scale SSF field operation, were inoculated with reovirus at ca. 1,000-times-greater concentrations than those typically found in domestic sewage. Reovirus removal and inactivation were investigated as functions of filter maturity and other filter sand characteristics. Reovirus removal studies demonstrated that the SSF process is capable of reducing reovirus in influent water by a minimum of 4 log concentration units under certain conditions of water quality, flow rate, and sand bed construction. Infectious reovirus was not detected in effluent samples from any of the sand beds studied, after inoculation of the SSF columns; therefore, removal efficiencies were not affected significantly by characteristics, including age, of the two filter sands evaluated. Studies conducted with radioactively labeled reovirus demonstrated that reovirus removed from influent water was distributed throughout the entire length of the filter beds. Concentrations of reovirus in the filter sands decreased with increasing bed depth. The greatest removal occurred in the top few centimeters of all sand beds. No infectious reovirus could be detected in clean or mature sand bed media, indicating that reoviruses were inactivated in the filter.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
McConnell LK,Sims RC,Barnett BBdoi
10.1128/AEM.48.4.818-825.1984subject
Has Abstractpub_date
1984-10-01 00:00:00pages
818-25issue
4eissn
0099-2240issn
1098-5336journal_volume
48pub_type
杂志文章abstract::Proteins that are produced for commercial purposes in Bacillus subtilis are commonly secreted via the Sec pathway. Despite its high secretion capacity, the secretion of heterologous proteins via the Sec pathway is often unsuccessful. Alternative secretion routes, like the Tat pathway, are therefore of interest. Two pa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01401-08
更新日期:2008-12-01 00:00:00
abstract::The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influenc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01067-06
更新日期:2006-11-01 00:00:00
abstract::A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01210-10
更新日期:2010-09-01 00:00:00
abstract::Denitrifying microbial communities and denitrification in salt marsh sediments may be affected by many factors, including environmental conditions, nutrient availability, and levels of pollutants. The objective of this study was to examine how microbial community composition and denitrification enzyme activities (DEA)...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01221-08
更新日期:2008-12-01 00:00:00
abstract::A light cycler-based real-time PCR (LC-PCR) assay that amplifies the F57 sequence of Mycobacterium avium subsp. paratuberculosis was developed. This assay also includes an internal amplification control template to monitor the amplification conditions in each reaction. The targeted F57 sequence element is unique for M...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.10.5957-5968.2005
更新日期:2005-10-01 00:00:00
abstract::The isoamylase gene from Flavobacterium odoratum KU was cloned into and expressed in Escherichia coli JM109. The promoter of the gene was successful in E. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. The enzyme found in the culture medium showed a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.9.4163-4170.1999
更新日期:1999-09-01 00:00:00
abstract::Three strains of Streptococcus bovis, a homolactic bacterium capable of utilizing starch, were evaluated for growth kinetics and ability to decrease the pH of alfalfa silage. A selected strain was evaluated for its competitiveness as an inoculant with Enterococcus faecium, an organism used in inoculants, and for its a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.10.3000-3005.1991
更新日期:1991-10-01 00:00:00
abstract::A novel, semicontinuous solid-phase fermentation system was used to produce fuel ethanol from sweet sorghum. The process was at an intermediate scale. In the process, dried and shredded sweet sorghum was rehydrated to 70% moisture, acidified to pH 2.0 to 3.0, and either pasteurized (12 h at 70 to 80 degrees C) or not ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.1.115-122.1986
更新日期:1986-01-01 00:00:00
abstract::During batch fermentation, the rate of ethanol production per milligram of cell protein is maximal for a brief period early in this process and declines progressively as ethanol accumulates in the surrounding broth. Our studies demonstrate that the removal of this accumulated ethanol does not immediately restore ferme...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.6.1286-1291.1987
更新日期:1987-06-01 00:00:00
abstract::Many fungal pathogens have short generation times, large population sizes, and mixed reproductive systems, providing high potential to adapt to heterogeneous environments of agroecosystems. Such adaptation complicates disease management and threatens food production. A better understanding of pathogen population biolo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02908-19
更新日期:2020-04-17 00:00:00
abstract::A European baseline survey during the years 2005 and 2006 has revealed that the monophasic Salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in German broiler flocks. In Denmark and the United Kingdom, its serovar prevalences were 15.15% and 2.8%...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02187-08
更新日期:2009-02-01 00:00:00
abstract::We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures between 33 and 39°C. W...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03890-15
更新日期:2016-01-15 00:00:00
abstract::Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by enzymes DszC, DszA, and DszB, which are encoded by the operon dszABC and contribute to the conversion in tandem. We investigated the expression characteristics of the dsz operon. Our results revealed that the levels of transcription and tran...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02319-07
更新日期:2008-02-01 00:00:00
abstract::Malic acid has great potential for replacing petrochemical building blocks in the future. For this application, high yields, rates, and titers are essential in order to sustain a viable biotechnological production process. Natural high-capacity malic acid producers like the malic acid producer Aspergillus flavus have ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01445-13
更新日期:2013-10-01 00:00:00
abstract::Two strains of mesophilic lactic acid bacteria, Streptococcus cremoris AM2 and Leuconostoc lactis CNRZ 1091, were grown in pure and mixed cultures in the presence or absence of citrate (15 mM) and at controlled (pH 6.5) or uncontrolled pH. Microbial cell densities at the end of growth, maximum growth rates, the pH dec...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.10.2527-2531.1988
更新日期:1988-10-01 00:00:00
abstract::Agromonas oligotrophica (Bradyrhizobium oligotrophicum) S58(T) is a nitrogen-fixing oligotrophic bacterium isolated from paddy field soil that is able to grow in extra-low-nutrient environments. Here, the complete genome sequence of S58 was determined. The S58 genome was found to comprise a circular chromosome of 8,26...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00009-13
更新日期:2013-04-01 00:00:00
abstract::A plasmid marker rescue system based on restoration of the nptII gene was established in Streptococcus gordonii to study the transfer of bacterial and transgenic plant DNA by transformation. In vitro studies revealed that the marker rescue efficiency depends on the type of donor DNA. Plasmid and chromosomal DNA of bac...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.10.6121-6127.2003
更新日期:2003-10-01 00:00:00
abstract::The bacterial second messengers (p)ppGpp and bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) regulate important functions, such as transcription, virulence, biofilm formation, and quorum sensing. In mycobacteria, they regulate long-term survival during starvation, pathogenicity, and dormancy. Recently, a Pseudomonas aerugin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03999-14
更新日期:2015-04-01 00:00:00
abstract::During malolactic fermentation in wine by Oenococcus oeni, the degradation of citric acid was delayed compared to the degradation of malic acid. The maximum concentration of diacetyl, an intermediary compound in the citric acid metabolism with a buttery or nutty flavor, coincided with the exhaustion of malic acid in t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.2.740-745.1999
更新日期:1999-02-01 00:00:00
abstract::The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-ter...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.5.1430-1436.1993
更新日期:1993-05-01 00:00:00
abstract::The pathogenicity of Listeria monocytogenes as influenced by growth on crabmeat at 5 and 10 degrees C was studied. Crabmeat was inoculated with L. monocytogenes V7 (ca. 10(4) CFU/g) and incubated for up to 14 days at 5 and 10 degrees C. At selected incubation times, L. monocytogenes was removed from crabmeat by washin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.5.1216-1220.1990
更新日期:1990-05-01 00:00:00
abstract::Microbial mercury methylation and methylmercury decomposition were examined in Lake Clara, an oligotrophic northern Wisconsin seepage lake, using radioisotopic tracers. Methylation activity was near background in the water column, was greatest in the profundal surficial sediments, and decreased with depth in sediment ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.10.2397-2404.1987
更新日期:1987-10-01 00:00:00
abstract::Waters in marine and freshwater areas of Puerto Rico were analyzed for the presence of Legionella spp. by direct fluorescent antibody assay with guinea pig confirmation. Several species, including L. bozemanii, L. dumoffii, L. gormanii, L. longbeachae, L. micdadei, and L. pneumophila, were widely distributed among all...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.9.2231-2236.1987
更新日期:1987-09-01 00:00:00
abstract::A simple and efficient process for the production of a ruminant feed supplement, rich in crude protein (defined as total N X 6.25), by bacterial fermentation of cheese whey has been developed. The lactose in unpasteurized whey is fermented to lactate acid by Lactobacillus bulgaricus at a temperature of 43 degrees C an...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.32.6.769-776.1976
更新日期:1976-12-01 00:00:00
abstract::Few techniques are currently available for quantifying specific prokaryotic taxa in environmental samples. Quantification of specific genotypes has relied mainly on oligonucleotide hybridization to extracted rRNA or intact rRNA in whole cells. However, low abundance and cellular rRNA content limit the application of t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.11.4605-4614.2000
更新日期:2000-11-01 00:00:00
abstract::A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previou...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.12.4230-4235.1995
更新日期:1995-12-01 00:00:00
abstract::Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL, to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PC...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02855-07
更新日期:2008-04-01 00:00:00
abstract::The combination of ecological diversity with genetic and experimental tractability makes Drosophila a powerful model for the study of animal-associated microbial communities. Despite the known importance of yeasts in Drosophila physiology, behavior, and fitness, most recent work has focused on Drosophila-bacterial int...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01741-12
更新日期:2012-10-01 00:00:00
abstract::Large surface-to-volume ratios provide optimal nutrient uptake conditions for small microorganisms in oligotrophic habitats. The surface area can be increased with appendages. Here, we describe chains of interconnecting vesicles protruding from cells of strain Hel3_A1_48, affiliating with Formosa spp. within the Flavo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00829-19
更新日期:2019-09-17 00:00:00
abstract::The effects of long-term heavy metal deposition on microbial community structure and the level of bacterial community tolerance were studied along two different gradients in Scandinavian coniferous forest soils. One was near the Harjavalta smelter in Finland, and one was at Ronnskar in Sweden. Phospholipid fatty acid ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.2.420-428.1996
更新日期:1996-02-01 00:00:00