Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection.

Abstract:

:The aim of this study was to compare the antagonistic properties of Lactobacillus casei GG exerted in vitro against Salmonella typhimurium C5 in a cellular model, cultured enterocyte-like Caco-2 cells, to those exerted in vivo in an animal model, C3H/He/Oujco mice. Our results show that a 1-h contact between the invading strain C5 and either the culture or the supernatant of L. casei GG impeded the invasion by the Salmonella strain in Caco-2 cells, without modifying the viability of the strain. After neutralization at pH 7, no inhibition of the invasion by C5 was observed. The antagonistic activity of L. casei GG was examined in C3H/He/Oujco mice orally infected with C5 as follows: (i) L. casei GG was given daily to conventional animals as a probiotic, and (ii) it was given once to germ-free animals in order to study the effect of the population of L. casei GG established in the different segments of the gut. In vivo experiments show that after a single challenge with C5, this strain survives and persists at a higher level in the feces of the untreated conventional mice than in those of the treated group. In L. casei GG germ-free mice, establishment of L. casei GG in the gut significantly delayed the occurrence of 100% mortality of the animals (15 days after C5 challenge versus 9 days in germ-free mice [P < 0.01]). Cecal colonization level and translocation rate of C5 to the mesenteric lymph nodes, spleen, and liver were significantly reduced during the first 2 days post-C5 challenge, although the L. casei GG population level in the gut dramatically decreased in these animals.

journal_name

Appl Environ Microbiol

authors

Hudault S,Liévin V,Bernet-Camard MF,Servin AL

doi

10.1128/AEM.63.2.513-518.1997

subject

Has Abstract

pub_date

1997-02-01 00:00:00

pages

513-8

issue

2

eissn

0099-2240

issn

1098-5336

journal_volume

63

pub_type

杂志文章
  • A novel sensitive bioassay for detection of Bacillus cereus emetic toxin and related depsipeptide ionophores.

    abstract::Of the toxins produced by Bacillus cereus, the emetic toxin is likely the most dangerous but, due to the lack of a suitable assay, the least well known. In this paper, a new, sensitive, inexpensive, and rapid bioassay for detection of the emetic toxin of B. cereus is described. The assay is based on the loss of motili...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.4.1338-1343.1998

    authors: Andersson MA,Mikkola R,Helin J,Andersson MC,Salkinoja-Salonen M

    更新日期:1998-04-01 00:00:00

  • Growth of methanogenic bacteria in pure culture with 2-propanol and other alcohols as hydrogen donors.

    abstract::Two types of mesophilic, methanogenic bacteria were isolated in pure culture from anaerobic freshwater and marine mud with 2-propanol as the hydrogen donor. The freshwater strain (SK) was a Methanospirillum species, the marine, salt-requiring strain (CV), which had irregular coccoid cells, resembled Methanogenium sp. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.5.1056-1062.1986

    authors: Widdel F

    更新日期:1986-05-01 00:00:00

  • Effects of carbon substrates on nitrite accumulation in freshwater sediments

    abstract::The contribution of the biochemical pathways nitrification, denitrification, and dissimilatory NO3- reduction to NH4+ (DNRA) to the accumulation of NO2- in freshwaters is governed by the species compositions of the bacterial populations resident in the sediments, available carbon (C) and nitrogen (N) substrates, and e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.1.61-66.1999

    authors: Kelso BHL,Smith RV,Laughlin RJ

    更新日期:1999-01-01 00:00:00

  • Microbiological comparison of surface soil and unsaturated subsurface soil from a semiarid high desert.

    abstract::Thirty-two chemoheterotrophic bacteria were isolated from unsaturated subsurface soil samples obtained from ca. 70 m below land surface in a high desert in southeastern Idaho. Most isolates were gram positive (84%) and strict aerobes (79%). Acridine orange direct counts of microbes in one subsurface sample showed lowe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.9.2420-2423.1989

    authors: Colwell FS

    更新日期:1989-09-01 00:00:00

  • Molecular definition of vaginal microbiota in East African commercial sex workers.

    abstract::Resistance to HIV infection in a cohort of commercial sex workers living in Nairobi, Kenya, is linked to mucosal and antiinflammatory factors that may be influenced by the vaginal microbiota. Since bacterial vaginosis (BV), a polymicrobial dysbiosis characterized by low levels of protective Lactobacillus organisms, is...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02943-10

    authors: Schellenberg JJ,Links MG,Hill JE,Dumonceaux TJ,Kimani J,Jaoko W,Wachihi C,Mungai JN,Peters GA,Tyler S,Graham M,Severini A,Fowke KR,Ball TB,Plummer FA

    更新日期:2011-06-01 00:00:00

  • Taking the starch out of oral biofilm formation: molecular basis and functional significance of salivary α-amylase binding to oral streptococci.

    abstract::α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-bi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.02581-12

    authors: Nikitkova AE,Haase EM,Scannapieco FA

    更新日期:2013-01-01 00:00:00

  • Immunofluorescent evidence of Proteus mirabilis swarm cell formation on sterilized rat feces.

    abstract::Swarming Proteus spp. were detected with the use of proteometry (a most-probable-number technique) in the fecal material of selected animal species and in raw sewage from a local sewage treatment plant. Proteus spp. were not detected in any of several soil and freshwater samples examined. Since rat feces harbored high...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.3.801-806.1981

    authors: Stahl ML,Williams FD

    更新日期:1981-03-01 00:00:00

  • Relationship between nitrogen-fixing sulfate reducers and fermenters in salt marsh sediments and roots of Spartina alterniflora.

    abstract::A combination of inhibitors and carbon substrates was used to determine the relative contribution of sulfate-reducing bacteria (SRB) and fermenting bacteria to nitrogen fixation in a salt marsh sediment and on the roots of Spartina alterniflora. Because a lag period precedes acetylene-reducing activity (ARA) in amende...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.8.2031-2036.1988

    authors: Gandy EL,Yoch DC

    更新日期:1988-08-01 00:00:00

  • Regulation of Botulinum Neurotoxin Synthesis and Toxin Complex Formation by Arginine and Glucose in Clostridium botulinum ATCC 3502.

    abstract::Botulinum neurotoxin (BoNT), produced by neurotoxigenic clostridia, is the most potent biological toxin known and the causative agent of the paralytic disease botulism. The nutritional, environmental, and genetic regulation of BoNT synthesis, activation, stability, and toxin complex (TC) formation is not well studied....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00642-17

    authors: Fredrick CM,Lin G,Johnson EA

    更新日期:2017-06-16 00:00:00

  • Characterization of Pseudomonas maltophilia Isolates from Fleece Rot.

    abstract::A total of 286 isolates of Pseudomonas maltophilia was collected from sheep exhibiting brown or yellowish fleece rot and from fly-strike lesions. Enzyme activities for 10 of the isolates were examined by plate tests and with the API ZYM system and compared with the enzymatic profile of a human type strain of P. maltop...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.2.346-348.1986

    authors: Macdiarmid JA,Burrell DH

    更新日期:1986-02-01 00:00:00

  • Characterization of mundticin L, a class IIa anti-Listeria bacteriocin from Enterococcus mundtii CUGF08.

    abstract::Enterococcus mundtii CUGF08, a lactic acid bacterium isolated from alfalfa sprouts, was found to produce mundticin L, a new class IIa bacteriocin that has a high level of inhibitory activity against the genus Listeria. The plasmid-associated operons containing genes for the mundticin L precursor, the ATP binding casse...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00752-09

    authors: Feng G,Guron GK,Churey JJ,Worobo RW

    更新日期:2009-09-01 00:00:00

  • Distributions of Fecal Markers in Wastewater from Different Climatic Zones for Human Fecal Pollution Tracking in Australian Surface Waters.

    abstract::Recreational and potable water supplies polluted with human wastewater can pose a direct health risk to humans. Therefore, sensitive detection of human fecal pollution in environmental waters is very important to water quality authorities around the globe. Microbial source tracking (MST) utilizes human fecal markers (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03765-15

    authors: Ahmed W,Sidhu JPS,Smith K,Beale DJ,Gyawali P,Toze S

    更新日期:2015-12-18 00:00:00

  • Ex vivo protocol for testing virus survival on human skin: experiments with herpesvirus 2.

    abstract::We report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. Using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. At the physiological skin temperature of 32 degrees C, the half-life ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.11.4252-4255.1996

    authors: Graham ML,Springthorpe VS,Sattar SA

    更新日期:1996-11-01 00:00:00

  • Iron-Stimulated N(2) Fixation and Growth in Natural and Cultured Populations of the Planktonic Marine Cyanobacteria Trichodesmium spp.

    abstract::In light of recent proposals that iron (Fe) availability may play an important role in controlling oceanic primary production and nutrient flux, its regulatory impact on N(2) fixation and production dynamics was investigated in the widespread and biogeochemically important diazotrophic, planktonic cyanobacteria Tricho...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.3.1044-1047.1994

    authors: Paerl HW,Prufert-Bebout LE,Guo C

    更新日期:1994-03-01 00:00:00

  • Developmental and microbiological analysis of the inception of bioluminescent symbiosis in the marine fish Nuchequula nuchalis (Perciformes: Leiognathidae).

    abstract::Many marine fish harbor luminous bacteria as bioluminescent symbionts. Despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01619-08

    authors: Dunlap PV,Davis KM,Tomiyama S,Fujino M,Fukui A

    更新日期:2008-12-01 00:00:00

  • Extremely acidophilic protists from acid mine drainage host Rickettsiales-lineage endosymbionts that have intervening sequences in their 16S rRNA genes.

    abstract::During a molecular phylogenetic survey of extremely acidic (pH < 1), metal-rich acid mine drainage habitats in the Richmond Mine at Iron Mountain, Calif., we detected 16S rRNA gene sequences of a novel bacterial group belonging to the order Rickettsiales in the Alphaproteobacteria. The closest known relatives of this ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.9.5512-5518.2003

    authors: Baker BJ,Hugenholtz P,Dawson SC,Banfield JF

    更新日期:2003-09-01 00:00:00

  • Unique substrate spectrum and PCR application of Nanoarchaeum equitans family B DNA polymerase.

    abstract::The known archaeal family B DNA polymerases are unable to participate in the PCR in the presence of uracil. Here, we report on a novel archaeal family B DNA polymerase from Nanoarchaeum equitans that can successfully utilize deaminated bases such as uracil and hypoxanthine and on its application to PCR. N. equitans fa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00624-08

    authors: Choi JJ,Song JG,Nam KH,Lee JI,Bae H,Kim GA,Sun Y,Kwon ST

    更新日期:2008-11-01 00:00:00

  • Elevated temperature enhances virulence of Erwinia carotovora subsp. carotovora strain EC153 to plants and stimulates production of the quorum sensing signal, N-acyl homoserine lactone, and extracellular proteins.

    abstract::Erwinia carotovora subsp. atroseptica, E. carotovora subsp. betavasculorum, and E. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt), and the quorum-sensing signal N-acyl-homoserine lactone (AHL) at 28 de...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4655-4663.2005

    authors: Hasegawa H,Chatterjee A,Cui Y,Chatterjee AK

    更新日期:2005-08-01 00:00:00

  • Engineering Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase for Fully Active Amidated NAD Biosynthesis.

    abstract::NAD and its reduced form NADH function as essential redox cofactors and have major roles in determining cellular metabolic features. NAD can be synthesized through the deamidated and amidated pathways, for which the key reaction involves adenylylation of nicotinic acid mononucleotide (NaMN) and nicotinamide mononucleo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00692-17

    authors: Wang X,Zhou YJ,Wang L,Liu W,Liu Y,Peng C,Zhao ZK

    更新日期:2017-06-16 00:00:00

  • Manganese Peroxidase, Produced by Trametes versicolor during Pulp Bleaching, Demethylates and Delignifies Kraft Pulp.

    abstract::Previous work has shown that Trametes (Coriolus) versicolor bleaches kraft pulp brownstock with the concomitant release of methanol. In this work, the fungus is shown to produce both laccase and manganese peroxidase (MnP) but not lignin peroxidase during pulp bleaching. MnP production was enhanced by the presence of p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.1.260-265.1993

    authors: Paice MG,Reid ID,Bourbonnais R,Archibald FS,Jurasek L

    更新日期:1993-01-01 00:00:00

  • Development and application of oligonucleotide probes for molecular identification of Xenorhabdus species.

    abstract::Synthetic deoxyoligonucleotide probes that hybridized against the region at positions 455 through 480 of 16S rRNA were developed for the identification of all five Xenorhabdus species. Sequence variation in the respective rRNA region between two strains of Xenorhabdus luminescens in addition allowed the construction o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.1.181-186.1990

    authors: Pütz J,Meinert F,Wyss U,Ehlers RU,Stackebrandt E

    更新日期:1990-01-01 00:00:00

  • A comparison of methods for counting viruses in aquatic systems.

    abstract::In this study, we compared different methods-including transmission electron microscopy-and various nucleic acid labeling methods in which we used the fluorochromes 4',6'-diamidino-2-phenylindole (DAPI), 4-[3-methyl-2,3-dihydro-(benzo-1, 3-oxazole)-2-methylmethyledene]-1-(3'-trimethyl ammoniumpropyl)-quinilinium diioi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.6.2283-2289.2000

    authors: Bettarel Y,Sime-Ngando T,Amblard C,Laveran H

    更新日期:2000-06-01 00:00:00

  • Genome-wide transcriptional variation within and between steady states for continuous growth of the hyperthermophile Thermotoga Maritima.

    abstract::Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates (80 degrees C/0.25 h(-1), 80 degrees C/0.17 h(-1), and 85 degrees C/0.25 h(-1)) showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.9.5572-5576.2005

    authors: Shockley KR,Scott KL,Pysz MA,Conners SB,Johnson MR,Montero CI,Wolfinger RD,Kelly RM

    更新日期:2005-09-01 00:00:00

  • Predictive model for inactivation of feline calicivirus, a norovirus surrogate, by heat and high hydrostatic pressure.

    abstract::Noroviruses, which are members of the Caliciviridae family, represent the leading cause of nonbacterial gastroenteritis in developed countries; such norovirus infections result in high economic costs for health protection. Person-to-person contact, contaminated water, and foods, especially raw shellfish, vegetables, a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01784-07

    authors: Buckow R,Isbarn S,Knorr D,Heinz V,Lehmacher A

    更新日期:2008-02-01 00:00:00

  • Magnetospirillum bellicus sp. nov., a novel dissimilatory perchlorate-reducing alphaproteobacterium isolated from a bioelectrical reactor.

    abstract::Previously isolated dissimilatory perchlorate-reducing bacteria (DPRB) have been primarily affiliated with the Betaproteobacteria. Enrichments from the cathodic chamber of a bioelectrical reactor (BER) inoculated from creek water in Berkeley, CA, yielded a novel organism most closely related to a previously described ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00015-10

    authors: Thrash JC,Ahmadi S,Torok T,Coates JD

    更新日期:2010-07-01 00:00:00

  • Response of a soil bacterial community to grassland succession as monitored by 16S rRNA levels of the predominant ribotypes.

    abstract::The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.3998-4003.2000

    authors: Felske A,Wolterink A,Van Lis R,De Vos WM,Akkermans AD

    更新日期:2000-09-01 00:00:00

  • Composition and enzymatic activity of the extracellular matrix secreted by germlings of botrytis cinerea.

    abstract::Germlings of Botrytis cinerea, an important fungal pathogen of plants, produce an extracellular matrix (ECM), or ensheathing film, that serves, in part, in their attachment (R. P. Doss, et al., Appl. Environ. Microbiol. 61:260-265, 1995). The composition of this film has been ascertained by using samples obtained by g...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.2.404-408.1999

    authors: Doss RP

    更新日期:1999-02-01 00:00:00

  • Diversity of two-domain laccase-like multicopper oxidase genes in Streptomyces spp.: identification of genes potentially involved in extracellular activities and lignocellulose degradation during composting of agricultural waste.

    abstract::Traditional three-domain fungal and bacterial laccases have been extensively studied for their significance in various biotechnological applications. Growing molecular evidence points to a wide occurrence of more recently recognized two-domain laccase-like multicopper oxidase (LMCO) genes in Streptomyces spp. However,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00223-14

    authors: Lu L,Zeng G,Fan C,Zhang J,Chen A,Chen M,Jiang M,Yuan Y,Wu H,Lai M,He Y

    更新日期:2014-06-01 00:00:00

  • Phylogenetic Relationships and Functional Genes: Distribution of a Gene (mnxG) encoding a putative manganese-oxidizing enzyme in Bacillus species.

    abstract::Several Bacillus and Paenibacillus species were isolated from Fe and Mn oxide minerals precipitating at a deep subsurface oxic-anoxic interface at Henderson Molybdenum Mine, Empire, CO. The isolates were investigated for their Mn(II)-oxidizing potential and interrogated for possession of the mnxG gene, a gene that cod...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00540-08

    authors: Mayhew LE,Swanner ED,Martin AP,Templeton AS

    更新日期:2008-12-01 00:00:00

  • "Endomicrobia": cytoplasmic symbionts of termite gut protozoa form a separate phylum of prokaryotes.

    abstract::Lignocellulose digestion by wood-feeding termites depends on the mutualistic interaction of unusual, flagellate protists located in their hindgut. Most of the flagellates harbor numerous prokaryotic endosymbionts of so-far-unknown identity and function. Using a full-cycle molecular approach, we show here that the endo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.3.1473-1479.2005

    authors: Stingl U,Radek R,Yang H,Brune A

    更新日期:2005-03-01 00:00:00