Development of a sensitive chemiluminometric assay for the detection of beta-galactosidase in permeabilized coliform bacteria and comparison with fluorometry and colorimetry.

abstract：:Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxyla...

journal_title：Applied and environmental microbiology

authors： Forsell JH,Kateley JR,Yoshizawa T,Pestka JJ

更新日期：1985-06-01 00:00:00

abstract：:The form of a previously developed Bĕlehrádek type of growth rate model was used to develop a probability model for defining the growth/no growth interface as a function of temperature (10 to 37 degrees C), pH (pH 2.8 to 6.9), lactic acid concentration (0 to 500 mM), and water activity (0.955 to 0.999; NaCl was used a...

journal_title：Applied and environmental microbiology

authors： Presser KA,Ross T,Ratkowsky DA

更新日期：1998-05-01 00:00:00

abstract：:Smectite clay minerals are abundant in soils and sediments worldwide and are typically rich in Fe. While recent investigations have shown that the structural Fe(III) bound in clay minerals is reduced by microorganisms, previous studies have not tested growth with clay minerals as the sole electron acceptor. Here we ha...

journal_title：Applied and environmental microbiology

authors： Kostka JE,Dalton DD,Skelton H,Dollhopf S,Stucki JW

更新日期：2002-12-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:This study was aimed at assessing whether the repeated exposure of 12 strains of Salmonella spp., Escherichia coli, and Listeria monocytogenes to alternative nonthermal decontamination techniques with UV light (UV-C) and nonthermal atmospheric plasma (NTAP) may cause the emergence of variants showing increased resista...

journal_title：Applied and environmental microbiology

authors： Álvarez-Molina A,de Toro M,Ruiz L,López M,Prieto M,Alvarez-Ordóñez A

更新日期：2020-04-17 00:00:00

abstract：:A cDNA expression library of Trichoderma reesei RutC-30 was constructed in the yeast Saccharomyces cerevisiae. Two genes, abf1 and bxl1, were isolated by screening the yeast library for extracellular alpha-L-arabinofuranosidase activity with the substrate p-nitrophenyl-alpha-L-arabinofuranoside. The genes abf1 and bxl...

journal_title：Applied and environmental microbiology

authors： Margolles-Clark E,Tenkanen M,Nakari-Setälä T,Penttilä M

更新日期：1996-10-01 00:00:00

abstract：UNLABELLED:The ability to sense and adapt to temperature fluctuation is critical to the aquatic survival, transmission, and infectivity of Vibrio cholerae, the causative agent of the disease cholera. Little information is available on the physiological changes that occur when V. cholerae experiences temperature shifts....

journal_title：Applied and environmental microbiology

authors： Townsley L,Sison Mangus MP,Mehic S,Yildiz FH

更新日期：2016-06-30 00:00:00

abstract：:The application of whole cells containing cytochrome P-450BM-3 monooxygenase [EC 1.14.14.1] for the bioconversion of long-chain saturated fatty acids to omega-1, omega-2, and omega-3 hydroxy fatty acids was investigated. We utilized pentadecanoic acid and studied its conversion to a mixture of 12-, 13-, and 14-hydroxy...

journal_title：Applied and environmental microbiology

authors： Schneider S,Wubbolts MG,Sanglard D,Witholt B

更新日期：1998-10-01 00:00:00

abstract：:The phenylurea herbicide diuron [N-(3,4-dichlorophenyl)-N,N-dimethylurea] is widely used in a broad range of herbicide formulations, and consequently, it is frequently detected as a major water contaminant in areas where there is extensive use. We constructed a linuron [N-(3,4-dichlorophenyl)-N-methoxy-N-methylurea]- ...

journal_title：Applied and environmental microbiology

authors： Sørensen SR,Albers CN,Aamand J

更新日期：2008-04-01 00:00:00

abstract：:Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

journal_title：Applied and environmental microbiology

authors： Moody SF,Tyler BM

更新日期：1990-08-01 00:00:00

abstract：:Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

journal_title：Applied and environmental microbiology

authors： Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

更新日期：2003-02-01 00:00:00

abstract：:An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorh...

journal_title：Applied and environmental microbiology

authors： Hunter RC,Beveridge TJ

更新日期：2005-05-01 00:00:00

abstract：:The milk supply is considered a primary route for a bioterrorism attack with Bacillus anthracis spores because typical high-temperature short-time (HTST) pasteurization conditions cannot inactivate spores. In the event of intentional contamination, an effective method to inactivate the spores in milk under HTST proces...

journal_title：Applied and environmental microbiology

authors： Xu S,Labuza TP,Diez-Gonzalez F

更新日期：2008-06-01 00:00:00

abstract：:Degradation of cotton cellulose by Trichoderma reesei endoglucanase I (EGI) and cellobiohydrolase II (CBHII) was investigated by analyzing the insoluble cellulose fragments remaining after enzymatic hydrolysis. Changes in the molecular-size distribution of cellulose after attack by EGI, alone and in combination with C...

journal_title：Applied and environmental microbiology

authors： Kleman-Leyer KM,Siika-Aho M,Teeri TT,Kirk TK

更新日期：1996-08-01 00:00:00

abstract：:A trans unsaturated fatty acid was found as a major constituent in the lipids of Pseudomonas putida P8. The fatty acid was identified as 9-trans-hexadecenoic acid by gas chromatography, argentation thin-layer chromatography, and infrared absorption spectrometry. Growing cells of P. putida P8 reacted to the presence of...

journal_title：Applied and environmental microbiology

authors： Heipieper HJ,Diefenbach R,Keweloh H

更新日期：1992-06-01 00:00:00

abstract：:Comparisons of animals bearing and lacking microorganisms can offer valuable insight into the interactions between animal hosts and their resident microbiota. Most hosts are naturally infected, and therefore, these comparisons require specific procedures (e.g., antibiotic treatment or physical exclusion of microorgani...

journal_title：Applied and environmental microbiology

authors： Ridley EV,Wong AC,Douglas AE

更新日期：2013-05-01 00:00:00

abstract：UNLABELLED:The l-isoaspartyl protein carboxyl methyltransferase (PCM) repairs protein damage resulting from spontaneous conversion of aspartyl or asparaginyl residues to isoaspartate and increases long-term stationary-phase survival of Escherichia coli under stress. In the course of studies intended to examine PCM func...

journal_title：Applied and environmental microbiology

authors： VandenBerg KE,Ahn S,Visick JE

更新日期：2016-08-15 00:00:00

abstract：:The production of pectolytic enzymes (pectate lyase [Pel] and polygalacturonase [Peh]), cellulase (Cel), and protease (Prt) is activated in the soft rot bacterium Erwinia carotovora subsp. carotovora by aepA (activator of extracellular protein production) and celery extract (Y. Liu, H. Murata, A. Chatterjee, and A. K....

journal_title：Applied and environmental microbiology

authors： Murata H,Chatterjee A,Liu Y,Chatterjee AK

更新日期：1994-09-01 00:00:00

abstract：:The phylogenetic diversity and species richness of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were examined with aquarium biofiltration systems. Species richness, deduced from rarefaction analysis, and diversity indices indicated that the phylogenetic diversity and species richness of AOA are greater than thos...

journal_title：Applied and environmental microbiology

authors： Urakawa H,Tajima Y,Numata Y,Tsuneda S

更新日期：2008-02-01 00:00:00

abstract：:Sulfate-reducing bacteria (SRB) in anoxic waters and sediments are the major producers of methylmercury in aquatic systems. Although a considerable amount of work has addressed the environmental factors that control methylmercury formation and the conditions that control bioavailability of inorganic mercury to SRB, li...

journal_title：Applied and environmental microbiology

authors： Ekstrom EB,Morel FM,Benoit JM

更新日期：2003-09-01 00:00:00

abstract：:Enterococci are one of the major facultative anaerobic bacterial groups that reside in the human gastrointestinal tract. In the present study, the composition of the enterococcal fecal flora in three healthy humans was analyzed before, during, and after the daily consumption of approximately 125 g of a raw-milk Chedda...

journal_title：Applied and environmental microbiology

authors： Gelsomino R,Vancanneyt M,Cogan TM,Swings J

更新日期：2003-01-01 00:00:00

abstract：:The use of antibiotic resistance genes in plasmids causes potential biosafety and clinical hazards, such as the possibility of horizontal spread of resistance genes or the rapid emergence of multidrug-resistant pathogens. This paper introduces a novel auxotrophy complementation system that allowed plasmids and host ce...

journal_title：Applied and environmental microbiology

authors： Dong WR,Xiang LX,Shao JZ

更新日期：2010-04-01 00:00:00

abstract：:Race 1 isolates of Fusarium oxysporum f. sp. lycopersici (FOL) are characterized by the presence of AVR1 in their genomes. The product of this gene, Avr1, triggers resistance in tomato cultivars carrying resistance gene I In FOL race 2 and race 3 isolates, AVR1 is absent, and hence they are virulent on tomato cultivar...

journal_title：Applied and environmental microbiology

authors： Biju VC,Fokkens L,Houterman PM,Rep M,Cornelissen BJC

更新日期：2017-02-01 00:00:00

abstract：:Two isolates of enterohemorrhagic Escherichia coli (EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and to other O104:H4 strains. This suggests that different O104:H4 EHEC strains were present several years prior to the...

journal_title：Applied and environmental microbiology

authors： Monecke S,Mariani-Kurkdjian P,Bingen E,Weill FX,Balière C,Slickers P,Ehricht R

更新日期：2011-12-01 00:00:00

abstract：UNLABELLED:Rhizobia are best known for nodulating legume roots and fixing atmospheric nitrogen for the host in exchange for photosynthates. However, the majority of the diverse strains of rhizobia do not form nodules on legumes, often because they lack key loci that are needed to induce nodulation. Nonnodulating rhizob...

journal_title：Applied and environmental microbiology

authors： Gano-Cohen KA,Stokes PJ,Blanton MA,Wendlandt CE,Hollowell AC,Regus JU,Kim D,Patel S,Pahua VJ,Sachs JL

更新日期：2016-08-15 00:00:00

abstract：:The effects of mineral fertilizer (NPK) and organic manure on the community structure of soil ammonia-oxidizing bacteria (AOB) was investigated in a long-term (16-year) fertilizer experiment. The experiment included seven treatments: organic manure, half organic manure N plus half fertilizer N, fertilizer NPK, fertili...

journal_title：Applied and environmental microbiology

authors： Chu H,Fujii T,Morimoto S,Lin X,Yagi K,Hu J,Zhang J

更新日期：2007-01-01 00:00:00

abstract：:Intact core tetraether membrane lipids of marine planktonic Crenarchaeota were quantified in water column-suspended particulate matter obtained from four depth intervals ( approximately 70, 500, 1,000 and 1,500 m) at seven stations in the northwestern Arabian Sea to investigate the distribution of the organisms at var...

journal_title：Applied and environmental microbiology

authors： Sinninghe Damsté JS,Rijpstra WI,Hopmans EC,Prahl FG,Wakeham SG,Schouten S

更新日期：2002-06-01 00:00:00

abstract：:Bacterial source tracking is used to apportion fecal pollution among putative sources. Within this context, library-independent markers are genetic or phenotypic traits that can be used to identify the host origin without a need for library-dependent classification functions. The objective of this project was to use m...

journal_title：Applied and environmental microbiology

authors： Soule M,Kuhn E,Loge F,Gay J,Call DR

更新日期：2006-03-01 00:00:00

abstract：:Data from analyses of continuous culture fermentation of insoluble cellulose by Ruminococcus albus 7 were used to derive constants for the rate of cellulose hydrolysis and fermentation, growth yield, and maintenance. Cellulose concentration was 1% in the nutrient reservoir, and hydraulic retention times of 0.5, 1.0, 1...

journal_title：Applied and environmental microbiology

authors： Pavlostathis SG,Miller TL,Wolin MJ

更新日期：1988-11-01 00:00:00

abstract：:An enrichment method using d-cycloserine was designed for the isolation of spontaneous mutants of Zymomonas mobilis deficient in glucose or fructose utilization. The mutants could easily be isolated since they represented 80 to 90% of the population after two and three enrichment cycles. Glucokinase and fructokinase a...

journal_title：Applied and environmental microbiology

authors： Ait-Abdelkader N,Pencreach G,Joset F,Baratti JC

更新日期：1996-03-01 00:00:00