Abstract:
:The gene encoding the beta-amylase of Bacillus cereus BQ10-S1 (SpoII) was cloned into Escherichia coli JM 109. A sequenced DNA fragment of 2,001 bp contains the beta-amylase gene. The N-terminal sequences (AVNGKG MNPDYKAYLMAPLKKI), the C-terminal sequences (SHTSSW), and the amino acid sequences of the five regions in the beta-amylase molecules were determined. The mature beta-amylase contains 514 amino acid residues with a molecular mass of 57,885 Da. The amino acid sequence homology with those of known beta-amylases was 52.7% for Bacillus polymyxa, 52.0% for Bacillus circulans, 43.4% for Clostridium thermosulfurogenes, 31.8% for Arabidopsis thaliana, 31.5% for barley, 29.9% for sweet potato, and 28.9% for soybean. Ten well-conserved regions were found between the N terminus and the area around residue 430, but the C-terminal region of 90 residues has no similarity with those of the plant beta-amylases. The homology search revealed that this C-terminal region has homology with C-terminal regions of the beta-amylase from C. thermosulfurogenes, some bacterial alpha-amylases, cyclodextrin glucanotransferase, and glucoamylase. Some of these sequences are known as the raw-starch-binding domain. These results suggest that B. cereus beta-amylase has an extra domain which has raw-starch-binding ability and that the domain has considerable sequence homology with those of other amylases or related enzymes from a wide variety of microorganisms.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Nanmori T,Nagai M,Shimizu Y,Shinke R,Mikami Bdoi
10.1128/AEM.59.2.623-627.1993subject
Has Abstractpub_date
1993-02-01 00:00:00pages
623-7issue
2eissn
0099-2240issn
1098-5336journal_volume
59pub_type
杂志文章abstract::Fluorescent antibodies (FAs) prepared against 16 ammonia-oxidizing nitrifying bacteria were examined as to cross-reactivity in heterologous FA staining tests. Virtually all cross-reactions to the seven Nitrosomonas FAs were confined to the Nitrosomonas isolates. The five Nitrosospira, three Nitrosolobus, and one Nitro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.4.589-593.1978
更新日期:1978-10-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.1.269-274.1990
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.36.1.178-185.1978
更新日期:1978-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.56.7.2268-2270.1990
更新日期:1990-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.11.4131-4134.1995
更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.54.1.62-68.1988
更新日期:1988-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.6.1201-1205.1979
更新日期:1979-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.6.2049-2058.1994
更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/aem.69.8.4697-4705.2003
更新日期:2003-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.7.2259-2261.1990
更新日期:1990-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.8.3136-3141.1995
更新日期:1995-08-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.01969-13
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.34.6.647-653.1977
更新日期:1977-12-01 00:00:00
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更新日期:1999-06-01 00:00:00
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更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.49.6.1374-1378.1985
更新日期:1985-06-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2008-11-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2014-04-01 00:00:00
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更新日期:2009-08-01 00:00:00
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更新日期:2015-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
abstract::Plant surfaces, colonized by numerous and diverse bacterial species, are often considered hot spots for horizontal gene transfer (HGT) between plants and bacteria. Plant DNA released during the degradation of plant tissues can persist and remain biologically active for significant periods of time, suggesting that soil...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00