Effect of parental growth on dynamics of conjugative plasmid transfer in the pea spermosphere.


:Plasmid transfer rates for the conjugative plasmid R388::Tn1721 from Pseudomonas cepacia (donor) to Pseudomonas fluorescens (recipient) on agar media, in broth, and in microcosms containing sterile or nonsterile soil, in the presence or absence of germinating pea seeds, were determined. Donors, recipients, and transconjugants were enumerated on selective media after 1 day on agar or in broth culture and over a 7-day period in soil or pea spermosphere microcosms. Donor and recipient growth rates and plasmid transfer rate constants [(gamma), where (gamma) = transconjugants (middot) (donors (middot) recipients)(sup-1) (middot) h(sup-1)] were calculated for three initial parental densities (10(sup4), 10(sup6), or 10(sup8) CFU/g or ml) in each system. For all initial density levels, values of (gamma) in agar and broth matings were higher than those in soil or in the pea spermosphere-rhizosphere microcosms. Values of (gamma) were not influenced by the pea spermosphere or by sterile or nonsterile conditions of the soil. However, (gamma) values in microcosm experiments were inversely related to initial parental density and were directly related to donor growth rates. Values of (gamma) averaged 4 x 10(sup-10), 4 x 10(sup-12), and 3 x 10(sup-14) when initial donor and recipient cell densities were 10(sup4), 10(sup6), and 10(sup8) CFU/g, respectively. These results suggest that the plasmid transfer rate constant is independent of parental cell density only when parental growth is not limited. In a resource-limited environment, intra- or interspecific competition may reduce the transfer rate by limiting parental growth.


Appl Environ Microbiol


Sudarshana P,Knudsen GR





Has Abstract


1995-08-01 00:00:00












  • Cold shock induction of thermal sensitivity in Listeria monocytogenes.

    abstract::Cold shock at 0 to 15 degrees C for 1 to 3 h increased the thermal sensitivity of Listeria monocytogenes. In a model broth system, thermal death time at 60 degrees C was reduced by up to 45% after L. monocytogenes Scott A was cold shocked for 3 h. The duration of the cold shock affected thermal tolerance more than did...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Miller AJ,Bayles DO,Eblen BS

    更新日期:2000-10-01 00:00:00

  • Criteria and methodology for identifying respiratory denitrifiers.

    abstract::Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this redu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Mahne I,Tiedje JM

    更新日期:1995-03-01 00:00:00

  • Soil Fungicides for Controlling Chytridiaceous Mycoparasites of Gigaspora margarita and Glomus fasciculatum.

    abstract::The percentage of azygospores of Gigaspora margarita with zoosporangia of chytridiaceous fungi (CF) was reduced significantly after agitating them in fenaminosulf before incubation in soil. Fenaminosulf did not affect zoosporangia development on chlamydospores of Glomus fasciculatum. Metalaxyl and ethazol were not eff...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sylvia DM,Schenck NC

    更新日期:1983-04-01 00:00:00

  • Survival of Vibrio parahaemolyticus after chilling in transport media: an explanation for divergent findings.

    abstract::Differing incidences of Vibrio parahaemolyticus reported by separate government agencies are attributable to sample handling and subsequent isolation techniques. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Clark AG

    更新日期:1977-11-01 00:00:00

  • Identification and analysis of the biosynthetic gene cluster encoding the thiopeptide antibiotic cyclothiazomycin in Streptomyces hygroscopicus 10-22.

    abstract::Thiopeptide antibiotics are an important class of natural products resulting from posttranslational modifications of ribosomally synthesized peptides. Cyclothiazomycin is a typical thiopeptide antibiotic that has a unique bridged macrocyclic structure derived from an 18-amino-acid structural peptide. Here we reported ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wang J,Yu Y,Tang K,Liu W,He X,Huang X,Deng Z

    更新日期:2010-04-01 00:00:00

  • Generation of a superoxide dismutase (SOD)-deficient mutant of Campylobacter coli: evidence for the significance of SOD in Campylobacter survival and colonization.

    abstract::The microaerophilic nature of Campylobacter species implies an inherent sensitivity towards oxygen and its reduction products, particularly the superoxide anion. The deleterious effects of exposure to superoxide radicals are counteracted by the activity of superoxide dismutase (SOD). We have shown previously that Camp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Purdy D,Cawthraw S,Dickinson JH,Newell DG,Park SF

    更新日期:1999-06-01 00:00:00

  • Free-living Rhizobium strain able to grow on n(2) as the sole nitrogen source.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Dreyfus BL,Elmerich C,Dommergues YR

    更新日期:1983-02-01 00:00:00

  • Transmission of Escherichia coli O157:H7 from contaminated manure and irrigation water to lettuce plant tissue and its subsequent internalization.

    abstract::The transmission of Escherichia coli O157:H7 from manure-contaminated soil and irrigation water to lettuce plants was demonstrated using laser scanning confocal microscopy, epifluorescence microscopy, and recovery of viable cells from the inner tissues of plants. E. coli O157:H7 migrated to internal locations in plant...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Solomon EB,Yaron S,Matthews KR

    更新日期:2002-01-01 00:00:00

  • Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene (ehxA) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lorenz SC,Monday SR,Hoffmann M,Fischer M,Kase JA

    更新日期:2016-10-14 00:00:00

  • Expression of the arginine deiminase pathway genes in Lactobacillus sakei is strain dependent and is affected by the environmental pH.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Rimaux T,Rivière A,Illeghems K,Weckx S,De Vuyst L,Leroy F

    更新日期:2012-07-01 00:00:00

  • Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Müller TA,Fleischmann T,van der Meer JR,Kohler HP

    更新日期:2006-07-01 00:00:00

  • Evidence for natural transfer of a tetracycline resistance gene between bacteria from the human colon and bacteria from the bovine rumen.

    abstract::Previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, pRDB5, from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B(1)4. We have now shown that naturally occurring gene transfer elements in Bacteroides species and Prevotell...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Shoemaker NB,Wang GR,Salyers AA

    更新日期:1992-04-01 00:00:00

  • l-Rhamnose Metabolism in Clostridium beijerinckii Strain DSM 6423.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Diallo M,Simons AD,van der Wal H,Collas F,Houweling-Tan B,Kengen SWM,López-Contreras AM

    更新日期:2019-02-20 00:00:00

  • Reduction of Escherichia coli O157:H7 populations in cattle by addition of colicin E7-producing E. coli to feed.

    abstract::A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which laste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Schamberger GP,Phillips RL,Jacobs JL,Diez-Gonzalez F

    更新日期:2004-10-01 00:00:00

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Dalton C,Goater AD,Pethig R,Smith HV

    更新日期:2001-02-01 00:00:00

  • Multilocus sequence typing and phylogenetic analyses of Pseudomonas aeruginosa Isolates from the ocean.

    abstract::Recent isolation of Pseudomonas aeruginosa strains from the open ocean and subsequent pulsed-field gel electrophoresis analyses indicate that these strains have a unique genotype (N. H. Khan, Y. Ishii, N. Kimata-Kino, H. Esaki, T. Nishino, M. Nishimura, and K. Kogure, Microb. Ecol. 53:173-186, 2007). We hypothesized t...

    journal_title:Applied and environmental microbiology

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    authors: Khan NH,Ahsan M,Yoshizawa S,Hosoya S,Yokota A,Kogure K

    更新日期:2008-10-01 00:00:00

  • Effect of plating medium on heat activation requirement of Clostridium botulinum spores.

    abstract::Clostridium botulinum 62A and ATCC 25763 spores required heat activation for maximum colony formation when plated on reinforced clostridial agar (BBL Microbiology Systems) but not when plated on botulinum assay medium. Spores from strains B-aphis and 53B did not exhibit heat activation when plated on either medium. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Montville TJ

    更新日期:1981-10-01 00:00:00

  • Solute transport proteins and the outer membrane protein NmpC contribute to heat resistance of Escherichia coli AW1.7.

    abstract::This study aimed to elucidate determinants of heat resistance in Escherichia coli by comparing the composition of membrane lipids, as well as gene expression, in heat-resistant E. coli AW1.7 and heat-sensitive E. coli GGG10 with or without heat shock. The survival of E. coli AW1.7 at late exponential phase was 100-fol...

    journal_title:Applied and environmental microbiology

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    authors: Ruan L,Pleitner A,Gänzle MG,McMullen LM

    更新日期:2011-05-01 00:00:00

  • Biogenic mineral production by a novel arsenic-metabolizing thermophilic bacterium from the Alvord Basin, Oregon.

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    authors: Ledbetter RN,Connon SA,Neal AL,Dohnalkova A,Magnuson TS

    更新日期:2007-09-01 00:00:00

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    journal_title:Applied and environmental microbiology

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    authors: Wang GS,Grammel H,Abou-Aisha K,Sägesser R,Ghosh R

    更新日期:2012-10-01 00:00:00

  • Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain.

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    authors: Ohtani N,Tomita M,Itaya M

    更新日期:2015-12-28 00:00:00

  • Characterization of rhizobial isolates of Phaseolus vulgaris by staircase electrophoresis of low-molecular-weight RNA.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Velázquez E,Martínez-Romero E,Rodríguez-Navarro DN,Trujillo ME,Daza A,Mateos PF,Martínez-Molina E,van Berkum P

    更新日期:2001-02-01 00:00:00

  • Mycoplasma agalactiae Secretion of β-(1→6)-Glucan, a Rare Polysaccharide in Prokaryotes, Is Governed by High-Frequency Phase Variation.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Gaurivaud P,Baranowski E,Pau-Roblot C,Sagné E,Citti C,Tardy F

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  • Production and characterization of antibodies against nivalenol tetraacetate.

    abstract::Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmuno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wang CR,Chu FS

    更新日期:1991-04-01 00:00:00

  • Preservation of Rhizobium viability and symbiotic infectivity by suspension in water.

    abstract::Three Rhizobium japonicum strains and two slow-growing cowpea-type Rhizobium strains were found to remain viable and able to rapidly modulate their respective hosts after being stored in purified water at ambient temperatures for periods of 1 year and longer. Three fast-growing Rhizobium species did not remain viable ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Crist DK,Wyza RE,Mills KK,Bauer WD,Evans WR

    更新日期:1984-05-01 00:00:00

  • Abundance and distribution of Legionellaceae in Puerto Rican waters.

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ortiz-Roque CM,Hazen TC

    更新日期:1987-09-01 00:00:00

  • Degradation of Pyridine by Micrococcus luteus Isolated from Soil.

    abstract::An organism capable of growth on pyridine was isolated from soil by enrichment culture techniques and identified as Micrococcus luteus. The organism oxidized pyridine for energy and released N contained in the pyridine ring as ammonium. The organism could not grow on mono- or disubstituted pyridinecarboxylic acids or ...

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    pub_type: 杂志文章


    authors: Sims GK,Sommers LE,Konopka A

    更新日期:1986-05-01 00:00:00

  • Diversity of Listeria species in urban and natural environments.

    abstract::A total of 442 Listeria isolates, including 234 Listeria seeligeri, 80 L. monocytogenes, 74 L. welshimeri, 50 L. innocua, and 4 L. marthii isolates, were obtained from 1,805 soil, water, and other environmental samples collected over 2 years from four urban areas and four areas representing natural environments. Liste...

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    pub_type: 杂志文章


    authors: Sauders BD,Overdevest J,Fortes E,Windham K,Schukken Y,Lembo A,Wiedmann M

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    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wanker E,Huber A,Schwab H

    更新日期:1995-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Herve-Jimenez L,Guillouard I,Guedon E,Boudebbouze S,Hols P,Monnet V,Maguin E,Rul F

    更新日期:2009-04-01 00:00:00