Biotransformation of the antimelanoma agent betulinic acid by Bacillus megaterium ATCC 13368.

abstract：:The lipopeptide, surfactin, is produced by Bacillus subtilis. A study has been made on large-scale production of this surfactant. A good yield was obtained from a glucose substrate fermentation by continuously removing the product by foam fractionation. The surfactin could be easily recovered from the collapsed foam b...

journal_title：Applied and environmental microbiology

authors： Cooper DG,Macdonald CR,Duff SJ,Kosaric N

更新日期：1981-09-01 00:00:00

abstract：:Growing bacteria have a high concentration of ribosomes to ensure sufficient protein synthesis, which is necessary for genome replication and cellular division. To elucidate whether metabolic activity of soil microorganisms is coupled with growth, we investigated the relationship between rRNA and DNA synthesis in a so...

journal_title：Applied and environmental microbiology

authors： Papp K,Hungate BA,Schwartz E

更新日期：2018-04-02 00:00:00

abstract：:Of 98 suspected food-borne Clostridium perfringens isolates obtained from a nationwide survey by the Food and Consumer Product Safety Authority in The Netherlands, 59 strains were identified as C. perfringens type A. Using PCR-based techniques, the cpe gene encoding enterotoxin was detected in eight isolates, showing ...

journal_title：Applied and environmental microbiology

authors： Xiao Y,Wagendorp A,Moezelaar R,Abee T,Wells-Bennik MH

更新日期：2012-10-01 00:00:00

abstract：:Primary cell cultures, a continuous cell line, and a diploid cell line were grown on an artificial capillary system. The cells were subsequently infected with Venezuelan equine encephalitis virus, and viral replication was studied. Extracellular fluids harvested from this system contained high titers of virus and were...

journal_title：Applied and environmental microbiology

authors： Johnson AD,Eddy GA,Gangemi JD,Ramsburg HH,Metzger JF

更新日期：1978-02-01 00:00:00

abstract：:A total of 52 strains of Lactobacillus acidophilus were examined for production of bacteriocins. A majority (63%) demonstrated inhibitory activity against all members of a four-species grouping of Lactobacillus leichmannii, Lactobacillus bulgaricus, Lactobacillus helveticus, and Lactobacillus lactis. Four L. acidophil...

journal_title：Applied and environmental microbiology

authors： Barefoot SF,Klaenhammer TR

更新日期：1983-06-01 00:00:00

abstract：:Eleven of 13 Enterobacteriaceae species tested grew in moist stored wheat, highlighting a potential risk of this energy-saving airtight storage method. When Hansenula anomala was coinoculated, all Enterobacteriaceae species were significantly inhibited after 2 months of storage, six of them to below the detection limi...

journal_title：Applied and environmental microbiology

authors： Olstorpe M,Schnürer J,Passoth V

更新日期：2012-01-01 00:00:00

abstract：:A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of inge...

journal_title：Applied and environmental microbiology

authors： Mackie RI,Heath S

更新日期：1979-09-01 00:00:00

abstract：:Mycobiota growing on food is often beneficial for the ripening and development of the specific flavor characteristics of the product, but it can also be harmful due to the production of undesirable compounds such as mycotoxins or antibiotics. Some of the fungi most frequently isolated from fermented and cured meat pro...

journal_title：Applied and environmental microbiology

authors： Laich F,Fierro F,Martín JF

更新日期：2002-03-01 00:00:00

abstract：:To investigate the biochemical basis of the differences in the insecticidal spectrum of Bacillus thuringiensis insecticidal crystal proteins (ICPs), we performed membrane binding and toxicity assays with three different ICPs and three lepidopteran species. The three ICPs have different toxicity patterns in the three s...

journal_title：Applied and environmental microbiology

authors： Van Rie J,Jansens S,Höfte H,Degheele D,Van Mellaert H

更新日期：1990-05-01 00:00:00

abstract：:Heat-treated cells of Rhizobium leguminosarum biovar trifolii strain 162X95 were used to produce monoclonal antibodies (MAbs). The fusion produced three cross-reactive MAbs and eight MAbs specific for the immunizing strain and a group of five other R. trifolii strains from the same geographic region where 162X95 was i...

journal_title：Applied and environmental microbiology

authors： Wright SF

更新日期：1990-07-01 00:00:00

abstract：:The effects of different organic substrates on the abilities of anaerobic sediment enrichments to reductively dechlorinate polychlorinated biphenyls (PCBs) were studied. Sediments collected from a site previously contaminated with PCBs were dosed with additional PCBs (Aroclor 1242; approximately 300 ppm [300 mug/g], s...

journal_title：Applied and environmental microbiology

authors： Nies L,Vogel TM

更新日期：1990-09-01 00:00:00

abstract：:Enterotoxin-positive (Ent+) and enterotoxin-negative (Ent-) strains of Clostridium perfringens were cultured in Duncan-Strong sporulation medium containing starch at 37 and 46 degrees C. At 37 degrees C, all strains degraded starch and sporulated well. However, only Ent- strains could hydrolyze starch, grow extensivel...

journal_title：Applied and environmental microbiology

authors： García-Alvarado JS,Rodriguez MA,Labbé RG

更新日期：1992-01-01 00:00:00

abstract：:The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjug...

journal_title：Applied and environmental microbiology

authors： Musovic S,Oregaard G,Kroer N,Sørensen SJ

更新日期：2006-10-01 00:00:00

abstract：:Three species of colonic bacteria can ferment the mucopolysaccharide chondroitin sulfate: Bacteroides ovatus, Bacteroides sp. strain 3452A (an unnamed DNA homology group), and B. thetaiotaomicron. Proteins associated with the utilization of chondroitin sulfate by B. thetaiotaomicron have been characterized previously....

journal_title：Applied and environmental microbiology

authors： Lipeski L,Guthrie EP,O'Brien M,Kotarski SF,Salyers AA

更新日期：1986-05-01 00:00:00

abstract：:The genes encoding NAD(+)-dependent alanine dehydrogenases (AlaDHs) (EC 1.4.1.1) from the Antarctic bacterial organisms Shewanella sp. strain Ac10 (SheAlaDH) and Carnobacterium sp. strain St2 (CarAlaDH) were cloned and expressed in Escherichia coli. Of all of the AlaDHs that have been sequenced, SheAlaDH exhibited the...

journal_title：Applied and environmental microbiology

authors： Galkin A,Kulakova L,Ashida H,Sawa Y,Esaki N

更新日期：1999-09-01 00:00:00

abstract：:Accumulating evidence suggests that pesticides have played a role in the increased rate of honey bee colony loss. One of the most commonly used pesticides in the United States is the neonicotinoid imidacloprid. Although the primary mode of action of imidacloprid is on the insect nervous system, it has also been shown ...

journal_title：Applied and environmental microbiology

authors： Raymann K,Motta EVS,Girard C,Riddington IM,Dinser JA,Moran NA

更新日期：2018-06-18 00:00:00

abstract：:A resting culture of Rhizopus arrhizus (ATCC 11145) transformed verrucarin A into 16-hydroxyverrucarin A, whereas R. arrhizus transformed verrucarin B into a mixture of 16-hydroxyverrucarin B and 3'-hydroxyverrucarin A. Relative to verrucarins A and B, the 16-hydroxy derivatives showed marked increases in activity, as...

journal_title：Applied and environmental microbiology

authors： Pavanasasivam G,Jarvis BB

更新日期：1983-08-01 00:00:00

abstract：:The leaf colonization strategies of two bacterial strains were investigated. The foliar pathogen Pseudomonas syringae pv. syringae strain B728a and the nonpathogen Pantoea agglomerans strain BRT98 were marked with a green fluorescent protein, and surface (epiphytic) and subsurface (endophytic) sites of bean and maize ...

journal_title：Applied and environmental microbiology

authors： Sabaratnam S,Beattie GA

更新日期：2003-02-01 00:00:00

abstract：:Three Rhizobium japonicum strains and two slow-growing cowpea-type Rhizobium strains were found to remain viable and able to rapidly modulate their respective hosts after being stored in purified water at ambient temperatures for periods of 1 year and longer. Three fast-growing Rhizobium species did not remain viable ...

journal_title：Applied and environmental microbiology

authors： Crist DK,Wyza RE,Mills KK,Bauer WD,Evans WR

更新日期：1984-05-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) released by infected individuals or present in human and hospital wastes can potentially cause contamination problems. The presence of HIV-1 was investigated in 16 environmental samples, including raw wastewater, sludge, final effluent, soil, and pond water, collected fr...

journal_title：Applied and environmental microbiology

authors： Ansari SA,Farrah SR,Chaudhry GR

更新日期：1992-12-01 00:00:00

abstract：:Anaerobic degradation of polycyclic aromatic hydrocarbons has been investigated mostly with naphthalene as a model compound. Naphthalene degradation by sulfate-reducing bacteria proceeds via carboxylation to 2-naphthoic acid, formation of a coenzyme A thioester, and subsequent reduction to 5,6,7,8-tetrahydro-2-naphtho...

journal_title：Applied and environmental microbiology

authors： Weyrauch P,Heker I,Zaytsev AV,von Hagen CA,Arnold ME,Golding BT,Meckenstock RU

更新日期：2020-07-20 00:00:00

abstract：:Anaerobic ammonium oxidation is a recent addition to the microbial nitrogen cycle, and its metabolic pathway, including the production and conversion of its intermediate hydrazine, is not well understood. Therefore, the effect of hydroxylamine addition on the hydrazine metabolism of anaerobic ammonium-oxidizing (anamm...

journal_title：Applied and environmental microbiology

authors： van der Star WR,van de Graaf MJ,Kartal B,Picioreanu C,Jetten MS,van Loosdrecht MC

更新日期：2008-07-01 00:00:00

abstract：:A straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (sMMO) activity on solid media. Such activity results in the development of a colored complex between 1-naphthol, which is formed when sMMO reacts with naphthalene, and o-dianisidine (tetrazotized). Methanotro...

journal_title：Applied and environmental microbiology

authors： Graham DW,Korich DG,LeBlanc RP,Sinclair NA,Arnold RG

更新日期：1992-07-01 00:00:00

abstract：:A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Tran B,Sangermano LR,Palmer CJ

更新日期：1994-07-01 00:00:00

abstract：:The insertion sequence IS629, which is highly prevalent in Escherichia coli O157:H7 genomes, was found to be absent in O157:H- strains, which are on a divergent pathway in the emergence of O157:H7. Although O157:H- is deficient in IS629, it permits IS629 transposition, with an excision frequency higher than that of an...

journal_title：Applied and environmental microbiology

authors： Rump LV,Fischer M,González-Escalona N

更新日期：2011-07-01 00:00:00

abstract：:We report the spontaneous formation of a stable mannitol-producing variant of Leuconostoc pseudomesenteroides. The mannitol-producing variant showed mannitol dehydrogenase activity which was absent in the parental strain. It was also able to use fructose and glucose simultaneously, whereas the parental strain showed d...

journal_title：Applied and environmental microbiology

authors： Grobben GJ,Peters SW,Wisselink HW,Weusthuis RA,Hoefnagel MH,Hugenholtz J,Eggink G

更新日期：2001-06-01 00:00:00

abstract：:Direct electron microscopy of bacteriophages adsorbed to a carbon film without prior enrichment by specific host strains or concentration by physical or chemical methods was used to study the morphological diversity of natural bacteriophage assemblages in a North German lake. All samples contained a mixture of morphol...

journal_title：Applied and environmental microbiology

authors： Demuth J,Neve H,Witzel KP

更新日期：1993-10-01 00:00:00

abstract：:A system of tangential-flow filtration was evaluated for use in the detection of Giardia cysts in drinking water. This method was more sensitive in recovering cysts than a frequently used wound-orlon system of through-filtration. ...

journal_title：Applied and environmental microbiology

authors： Isaac-Renton JL,Fung CP,Lochan A

更新日期：1986-08-01 00:00:00

abstract：:The bioconversion of waste paper to single-cell protein at pH <1 by Scytalidium acidophilum is described. Waste paper pretreated with 72% H(2)SO(4) at 4 degrees C was diluted with water to a pH of <0.1 and hydrolyzed. This yielded an adequate sugar-containing substrate for the growth of the fungus. A total of 97% of t...

journal_title：Applied and environmental microbiology

authors： Ivarson KC,Morita H

更新日期：1982-03-01 00:00:00

abstract：:Solubilization of coal was demonstrated with filtrates (0.45-mum-pore-size filters) obtained from the broth in which Polyporus versicolor had grown. The rate and extent of solubilization were dependent on the age of the fungal cultures, the particle size of the coal, the pH of the filtrates, and the presence of protei...

journal_title：Applied and environmental microbiology

authors： Cohen MS,Bowers WC,Aronson H,Gray ET

更新日期：1987-12-01 00:00:00