Microbial catabolism of vanillate: decarboxylation to guaiacol.

Abstract:

:A novel catabolic transformation of vanillic acid (4-hydroxy-3-methoxybenzoic acid) by microorganisms is reported. Several strains of Bacillus megaterium and a strain of Streptomyces are shown to convert vanillate to guaiacol (o-methoxyphenol) and CO2 by nonoxidative decarboxylation. Use of a modified most-probable-number procedure shows that numerous soils contain countable numbers (10(1) to 10(2) organisms per g of dry soil) of aerobic sporeformers able to convert vanillate to guaiacol. Conversion of vanillate to guaiacol by the microfloras of most-probable-number replicates was used as the criterion for scoring replicates positive or negative. Guaiacol was detected by thin-layer chromatography. These results indicate that the classic separations of catabolic pathways leading to specific ring-fashion substrates such as protocatechuate and catechol are often interconnectable by single enzymatic transformations, usually a decarboxylation.

journal_name

Appl Environ Microbiol

authors

Crawford RL,Olson PP

doi

10.1128/AEM.36.4.539-543.1978

keywords:

subject

Has Abstract

pub_date

1978-10-01 00:00:00

pages

539-43

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

36

pub_type

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