Heat resistance of bacterial spores correlated with protoplast dehydration, mineralization, and thermal adaptation.

Abstract:

:Twenty-eight types of lysozyme-sensitive spores among seven Bacillus species representative of thermophiles, mesophiles, and psychrophiles were obtained spanning a 3,000-fold range in moist-heat resistance. The resistance within species was altered by demineralization of the native spores to protonated spores and remineralization of the protonated spores to calcified spores and by thermal adaptation at maximum, optimum, and minimum sporulation temperatures. Protoplast wet densities, and thereby protoplast water contents, were obtained by buoyant density sedimentation in Nycodenz gradients (Nyegaard and Co., Oslo, Norway). Increases in mineralization and thermal adaptation caused reductions in protoplast water content between limits of ca. 57 and 28% (wet weight basis), and thereby correlated with increases in sporal heat resistance. Above and below these limits, however, increases in mineralization and thermal adaptation correlated with increases in sporal resistance independently of unchanged protoplast water contents. All three factors evidently contributed to and were necessary for heat resistance of the spores, but dehydration predominated.

journal_name

Appl Environ Microbiol

authors

Beaman TC,Gerhardt P

doi

10.1128/AEM.52.6.1242-1246.1986

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

1242-6

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

52

pub_type

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