Rapid-viability PCR method for detection of live, virulent Bacillus anthracis in environmental samples.

abstract：:PCR generated two distinct products from a toxic isolate of Alexandrium catenella, which had been taken from Dai Ya Bay (southern China), by using primers for large-subunit rRNA. This pattern is distinct from published data for North American Alexandrium species. Sequences of the two products suggest that the smaller ...

journal_title：Applied and environmental microbiology

authors： Yeung PK,Kong KF,Wong FT,Wong JT

更新日期：1996-11-01 00:00:00

abstract：:Cryptosporidium parvum is an apicomplexan parasite that infects humans and ruminants. C. parvum isolated from cattle in northeastern Turkey and in Israel was genotyped using multiple polymorphic genetic markers, and the two populations were compared to assess the effect of cattle husbandry on the parasite's population...

journal_title：Applied and environmental microbiology

authors： Tanriverdi S,Markovics A,Arslan MO,Itik A,Shkap V,Widmer G

更新日期：2006-04-01 00:00:00

abstract：:The microaerophilic bacterium Campylobacter jejuni is the most common cause of bacterial food-borne infections in the developed world. Tolerance to environmental stress relies on proteases and chaperones in the cell envelope, such as HtrA and SurA. HtrA displays both chaperone and protease activities, but little is kn...

journal_title：Applied and environmental microbiology

authors： Baek KT,Vegge CS,Skórko-Glonek J,Brøndsted L

更新日期：2011-01-01 00:00:00

abstract：:Nonhomologous end joining (NHEJ) is critical for genome stability because of its roles in double-strand break repair. Ku and ligase D (LigD) are the crucial proteins in this process, and strains expressing Ku and LigD can cyclize linear DNA in vivo Here, we established a proof-of-concept single-homology-arm linear DNA...

journal_title：Applied and environmental microbiology

authors： Lu S,Nie Y,Wang M,Xu HX,Ma DL,Liang JL,Wu XL

更新日期：2018-09-17 00:00:00

abstract：:Pleurotus ostreatus ;Florida' was grown in submerged liquid culture. The biomass yield of the fungus, grown for 3 days in 2-liter fermentors, where the mycelial pellets measuring 5 mm in diameter were formed, was 11.7 g (dry weight)/liter. Comparing the chemical constituents of fruiting bodies produced on cotton straw...

journal_title：Applied and environmental microbiology

authors： Hadar Y,Cohen-Arazi E

更新日期：1986-06-01 00:00:00

abstract：:Listeria monocytogenes is a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. To probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700 L. monocytogenes mutants was screened, using a micro...

journal_title：Applied and environmental microbiology

authors： Hingston PA,Piercey MJ,Truelstrup Hansen L

更新日期：2015-08-15 00:00:00

abstract：:Azospirillum lipoferum 4T has original properties such as nonmotility, melanin synthesis, and laccase activity. Following random Tn5 mutagenesis in A. lipoferum 4T, we obtained 10 mutants which were affected in melanization and laccase activity. The class 1 mutants, with intermediate levels of laccase activity, showed...

journal_title：Applied and environmental microbiology

authors： Faure D,Bouillant ML,Bally R

更新日期：1994-09-01 00:00:00

abstract：:The fall armyworm, Spodoptera frugiperda, is an invasive maize pest that has spread from the Americas into Africa and Asia and causes severe crop damage worldwide. Most populations of S. frugiperda show low susceptibility to Bacillus thuringiensis (Bt) Cry1Ab or Cry1Ac toxins, which have been proved to be effective ag...

journal_title：Applied and environmental microbiology

authors： Gómez I,Ocelotl J,Sánchez J,Aguilar-Medel S,Peña-Chora G,Lina-Garcia L,Bravo A,Soberón M

更新日期：2020-10-28 00:00:00

abstract：:Type III protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. Certain type III protein secretion genes (e.g., hrcC) have been found in Pseudomonas sp. strain SBW25 (and other biocontrol pseudomonads), but other ty...

journal_title：Applied and environmental microbiology

authors： Rezzonico F,Défago G,Moënne-Loccoz Y

更新日期：2004-09-01 00:00:00

abstract：:A simple and rapid radioimmunoassay was developed for the quantitative determination of ochratoxin A in barley. [14C]ochratoxin A, with a specific activity of 130 Ci/mol, was used as the tracer. Toxin levels below 100 ng/ml required a cleanup step. Three methods (the Association of Official Analytical Chemists cleanup...

journal_title：Applied and environmental microbiology

authors： Rousseau DM,Slegers GA,Van Peteghem CH

更新日期：1985-08-01 00:00:00

abstract：:The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...

journal_title：Applied and environmental microbiology

authors： Dorella FA,Estevam EM,Pacheco LG,Guimarães CT,Lana UG,Gomes EA,Barsante MM,Oliveira SC,Meyer R,Miyoshi A,Azevedo V

更新日期：2006-11-01 00:00:00

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:Acinetobacter sp. strain P6 has previously been reported to utilize biphenyl (BP) and chlorinated BPs, with accumulation of corresponding chlorobenzoic acids. Arthrobacter sp. strain M5 was isolated as a contaminant in the culture of Acinetobacter sp. strain P6 growing on 4-chlorobiphenyl and showed properties similar...

journal_title：Applied and environmental microbiology

authors： Furukawa K,Chakrabarty AM

更新日期：1982-09-01 00:00:00

abstract：:Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts attached to flagellated protists. Spirochetes in the gut microbial community investigated so far are related to the genus...

journal_title：Applied and environmental microbiology

authors： Noda S,Ohkuma M,Yamada A,Hongoh Y,Kudo T

更新日期：2003-01-01 00:00:00

abstract：:Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipi...

journal_title：Applied and environmental microbiology

authors： Martínez B,Böttiger T,Schneider T,Rodríguez A,Sahl HG,Wiedemann I

更新日期：2008-08-01 00:00:00

abstract：:A rich history of investigation documents various Drosophila-yeast mutualisms, suggesting that Drosophila suzukii similarly has an association with a specific yeast species or community. To discover candidate yeast species, yeasts were isolated from larval frass, adult midguts, and fruit hosts of D. suzukii. Terminal ...

journal_title：Applied and environmental microbiology

authors： Hamby KA,Hernández A,Boundy-Mills K,Zalom FG

更新日期：2012-07-01 00:00:00

abstract：:This study examined the inactivation of human norovirus (HuNoV) GI.1 and GII.4 by chlorine under conditions mimicking sewage treatment. Using a porcine gastric mucin-magnetic bead (PGM-MB) assay, no statistically significant loss in HuNoV binding (inactivation) was observed for secondary effluent treatments of ≤25 ppm...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Fay JP,Calci K,Pouillot R,Woods J,Chen H,Niemira BA,Van Doren JM

更新日期：2017-11-16 00:00:00

abstract：:Many fungal pathogens have short generation times, large population sizes, and mixed reproductive systems, providing high potential to adapt to heterogeneous environments of agroecosystems. Such adaptation complicates disease management and threatens food production. A better understanding of pathogen population biolo...

journal_title：Applied and environmental microbiology

authors： Kozhar O,Larsen MM,Grünwald NJ,Peever TL

更新日期：2020-04-17 00:00:00

abstract：:AvrPtoB is a type III effector protein from Pseudomonas syringae pv. tomato that physically interacts with the tomato Pto kinase and, depending on the host genotype, either elicits or suppresses programmed cell death associated with plant immunity. We reported previously that avrPtoB-related sequences are present in d...

journal_title：Applied and environmental microbiology

authors： Lin NC,Abramovitch RB,Kim YJ,Martin GB

更新日期：2006-01-01 00:00:00

abstract：:Selective growth inhibitors of Sphaerotilus natans were detected in the culture broths of several strains of Streptomyces. Anslimins A and B were isolated from the culture broth of Streptomyces oganonensis by adsorption on resinous adsorbent followed by elution with 50% aqueous acetone. The A and B components were the...

journal_title：Applied and environmental microbiology

authors： Takiguchi Y,Yoshikawa H,Terao M

更新日期：1980-06-01 00:00:00

abstract：:The obligate intracellular growth of Rickettsia prowazekii places severe restrictions on the analysis of rickettsial gene expression. With a small genome, predicted to code for 835 proteins, identifying which proteins are differentially expressed in rickettsiae that are isolated from different hosts or that vary in vi...

journal_title：Applied and environmental microbiology

authors： Tucker AM,Driskell LO,Pannell LK,Wood DO

更新日期：2011-07-01 00:00:00

abstract：UNLABELLED:In the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P. putida have been investigated by generating single-, double-, and triple-null mut...

journal_title：Applied and environmental microbiology

authors： Huertas-Rosales Ó,Ramos-González MI,Espinosa-Urgel M

更新日期：2016-08-30 00:00:00

abstract：:An agar medium containing 5% cholesterol has been developed for the isolation, enumeration, and characterization of cholesterol-reducing strains of Eubacterium. ...

journal_title：Applied and environmental microbiology

authors： Brinkley AW,Gottesman AR,Mott GE

更新日期：1980-12-01 00:00:00

abstract：:During submerged culture in the presence of glucose and glutamate, the filamentous fungus Monascus ruber produces water-soluble red pigments together with citrinin, a mycotoxin with nephrotoxic and hepatoxic effects on animals. Analysis of the (13)C-pigment molecules from mycelia cultivated with [1-(13)C]-, [2-(13)C]-...

journal_title：Applied and environmental microbiology

authors： Hajjaj H,Klaébé A,Goma G,Blanc PJ,Barbier E,François J

更新日期：2000-03-01 00:00:00

abstract：:Genomic probes were used to investigate hepatitis A virus (HAV) and enterovirus RNAs in two types of shellfish from natural beds (Atlantic coast, France). After elution concentration, nucleic acid extracted by proteinase K and purified by phenol-chloroform and ethanol precipitation was assayed by dot blot hybridizatio...

journal_title：Applied and environmental microbiology

authors： Le Guyader F,Apaire-Marchais V,Brillet J,Billaudel S

更新日期：1993-11-01 00:00:00

abstract：:In this work, we shed light on the metabolism of dihydroxyacetone (DHA), a versatile, ubiquitous, and important intermediate for various chemicals in industry, by analyzing its metabolism at the system level in Escherichia coli Using constraint-based modeling, we show that the growth of E. coli on DHA is suboptimal an...

journal_title：Applied and environmental microbiology

authors： Peiro C,Millard P,de Simone A,Cahoreau E,Peyriga L,Enjalbert B,Heux S

更新日期：2019-07-18 00:00:00

abstract：:While screening aerobic, heterotrophic marine bacteria for production of volatile organic compounds, we found that a group of isolates produced substantial amounts of acetone. Acetone production was confirmed by gas chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography. The m...

journal_title：Applied and environmental microbiology

authors： Nemecek-Marshall M,Wojciechowski C,Kuzma J,Silver GM,Fall R

更新日期：1995-01-01 00:00:00

abstract：:DNA primers corresponding to conserved motifs in bacterial repetitive (REP, ERIC, and BOX) elements and PCR were used to show that REP-, ERIC-, and BOX-like DNA sequences are widely distributed in phytopathogenic Xanthomonas and Pseudomonas strains. REP-, ERIC, and BOX-PCR (collectively known as rep-PCR) were used to ...

journal_title：Applied and environmental microbiology

authors： Louws FJ,Fulbright DW,Stephens CT,de Bruijn FJ

更新日期：1994-07-01 00:00:00

abstract：:The abundance of nifH, nirS, and nirK gene fragments involved in nitrogen (N) fixation and denitrification in thinned second-growth Douglas-fir (Pseudotsuga menziesii subsp. menziesii [Mirb.] Franco) forest soil was investigated by using quantitative real-time PCR. Prokaryotic N cycling is an important aspect of N ava...

journal_title：Applied and environmental microbiology

authors： Levy-Booth DJ,Winder RS

更新日期：2010-11-01 00:00:00

abstract：:Examination of Listeria monocytogenes prevalence among ready-to-eat foods in Japan revealed frequent (5.7 to 12.1%) contamination of minced tuna and fish roe products, and the isolates had the same virulence levels as clinical isolates in terms of invasion efficiency and infectivity in cell cultures and a murine infec...

journal_title：Applied and environmental microbiology

authors： Miya S,Takahashi H,Ishikawa T,Fujii T,Kimura B

更新日期：2010-05-01 00:00:00