Serotyping, stx2 subtyping, and characterization of the locus of enterocyte effacement island of shiga toxin-producing Escherichia coli and E. coli O157:H7 strains isolated from the environment in France.

abstract：:This work demonstrates the ability of a bacterial concentration and recovery procedure combined with three different PCR assays targeting the lacZ, wecG, and 16S rRNA genes, respectively, to detect the presence of total coliforms in 100-ml samples of potable water (presence/absence test). PCR assays were first compare...

journal_title：Applied and environmental microbiology

authors： Maheux AF,Boudreau DK,Bisson MA,Dion-Dupont V,Bouchard S,Nkuranga M,Bergeron MG,Rodriguez MJ

更新日期：2014-07-01 00:00:00

abstract：:Rapid pathogen detection is crucial for the timely introduction of therapeutics. Two groups (one in the United Kingdom and one in the United States) independently evaluated inhibitor-resistant PCR reagents for the direct testing of substrates. In the United Kingdom, a multiplexed Bacillus anthracis (target) and Bacill...

journal_title：Applied and environmental microbiology

authors： Minogue TD,Rachwal PA,Trombley Hall A,Koehler JW,Weller SA

更新日期：2014-02-01 00:00:00

abstract：:Hepatitis A virus (HAV) and Norwalk-like virus (NLV) were detected by reverse transcription-PCR in clams imported into the United States from China. An epidemiological investigation showed that these clams were associated with five cases of Norwalk-like gastroenteritis in New York State in August 2000 (Food and Drug A...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Meade GK,Richards GP

更新日期：2002-08-01 00:00:00

abstract：:Previous studies have demonstrated that Staphylococcus epidermidis isolates colonizing the skin of healthy humans do not typically encode icaADBC, the genes responsible for the production of polysaccharide intercellular adhesin or biofilms. It was therefore hypothesized that the presence of icaADBC was deleterious to ...

journal_title：Applied and environmental microbiology

authors： Rogers KL,Rupp ME,Fey PD

更新日期：2008-10-01 00:00:00

abstract：:A census of clean room surface-associated bacterial populations was derived from the results of both the cloning and sequencing of 16S rRNA genes and DNA microarray (PhyloChip) analyses. Samples from the Lockheed Martin Aeronautics Multiple Testing Facility (LMA-MTF), the Kennedy Space Center Payload Hazard and Servic...

journal_title：Applied and environmental microbiology

authors： La Duc MT,Osman S,Vaishampayan P,Piceno Y,Andersen G,Spry JA,Venkateswaran K

更新日期：2009-10-01 00:00:00

abstract：:Lactobacillus bulgaricus LT4(0448) is a lysogenic strain from which a temperate bacteriophage can be induced by mitomycin C or UV irradiation. Lactobacillus lactis CNRZ 326 is an indicator strain for the temperate phage 0448, but this strain lyses only in the presence of Ca ions. A resistant culture developed secondar...

journal_title：Applied and environmental microbiology

authors： Cluzel PJ,Serio J,Accolas JP

更新日期：1987-08-01 00:00:00

abstract：:A large, obligately anaerobic spirochete (strain PB) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. The helical cells measured 0.6 to 0.7 micron by 12 to 20 micron and possessed approximately 16 periplasmic flagella inserted near each end of the protoplasmic cylinder. The ...

journal_title：Applied and environmental microbiology

authors： Paster BJ,Canale-Parola E

更新日期：1985-08-01 00:00:00

abstract：:Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...

journal_title：Applied and environmental microbiology

authors： Van Dyck PJ,Tobback P,Feyes M,van de Voorde H

更新日期：1982-06-01 00:00:00

abstract：:A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino...

journal_title：Applied and environmental microbiology

authors： Ishige T,Tani A,Sakai Y,Kato N

更新日期：2000-08-01 00:00:00

abstract：:Effects of the sesquiterpene farnesol (3,7,11-trimethyl-2,6,10-dodecatrien-1-ol) and the sesquiterpene derivative geosmin (1,10-trans-dimethyl-trans-9-decalol) were investigated in a geosmin-producing actinomycete, Streptomyces tendae. Exposure to 300 microM farnesol reduced biomass (fresh matter) accumulation by 97% ...

journal_title：Applied and environmental microbiology

authors： Dionigi CP,Millie DF,Johnsen PB

更新日期：1991-12-01 00:00:00

abstract：:Toxin-producing isolates of Bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. The toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic ...

journal_title：Applied and environmental microbiology

authors： Salkinoja-Salonen MS,Vuorio R,Andersson MA,Kämpfer P,Andersson MC,Honkanen-Buzalski T,Scoging AC

更新日期：1999-10-01 00:00:00

abstract：:Because of high diurnal water quality fluctuations in raw municipal wastewater, the use of proportional autosampling over a period of 24 h at municipal wastewater treatment plants (WWTPs) to evaluate carbon, nitrogen, and phosphorus removal has become a standard in many countries. Microbial removal or load estimation ...

journal_title：Applied and environmental microbiology

authors： Mayer RE,Vierheilig J,Egle L,Reischer GH,Saracevic E,Mach RL,Kirschner AK,Zessner M,Sommer R,Farnleitner AH

更新日期：2015-08-01 00:00:00

abstract：:The respiration percentage of H-labeled glucose by heterotrophic microorganisms in water samples was determined by a method based on equimolar addition of [H]- and [C]glucose to samples. The respiration percentage of [H]glucose exceeded that of [C]glucose by 10% over the assayed range of respiration rates. Respiration...

journal_title：Applied and environmental microbiology

authors： Kuparinen J,Tamminen T

更新日期：1982-04-01 00:00:00

abstract：:This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of ...

journal_title：Applied and environmental microbiology

authors： Tanaka S,Yamamoto K,Yamada K,Furuya K,Uyeno Y

更新日期：2016-04-18 00:00:00

abstract：:[This corrects the article on p. 1301 in vol. 50.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1986-03-01 00:00:00

abstract：:Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aur...

journal_title：Applied and environmental microbiology

authors： Synnott AJ,Kuang Y,Kurimoto M,Yamamichi K,Iwano H,Tanji Y

更新日期：2009-07-01 00:00:00

abstract：:The nprA gene, encoding Bacillus thuringiensis neutral protease A, was cloned by the use of gene-specific oligonucleotides. The size of neutral protease A deduced from the nprA sequence was 566 amino acids (60,982 Da). The cloned nprA gene was partially deleted in vitro, and the deleted allele, designated nprA3, was u...

journal_title：Applied and environmental microbiology

authors： Donovan WP,Tan Y,Slaney AC

更新日期：1997-06-01 00:00:00

abstract：:Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilm...

journal_title：Applied and environmental microbiology

authors： Ganeshnarayan K,Shah SM,Libera MR,Santostefano A,Kaplan JB

更新日期：2009-03-01 00:00:00

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:Listeria monocytogenes is among the most important food-borne pathogens and is well adapted to persist in the environment. To gain insight into the genetic relatedness and potential virulence of L. monocytogenes strains causing central nervous system (CNS) infections, we used multilocus variable-number tandem-repeat a...

journal_title：Applied and environmental microbiology

authors： Balandyté L,Brodard I,Frey J,Oevermann A,Abril C

更新日期：2011-12-01 00:00:00

abstract：:The utilization of the fuel oxygenate methyl tert-butyl ether (MTBE) and related compounds by microorganisms was investigated in a mainly theoretical study based on the Y(ATP) concept. Experiments were conducted to derive realistic maintenance coefficients and K(s) values needed to calculate substrate fluxes available...

journal_title：Applied and environmental microbiology

authors： Müller RH,Rohwerder T,Harms H

更新日期：2007-03-01 00:00:00

abstract：:Short-chain fatty acid (SCFA) biosynthesis is pertinent to production of biofuels, industrial compounds, and pharmaceuticals from renewable resources. To expand on Escherichia coli SCFA products, we previously implemented a coenzyme A (CoA)-dependent pathway that condenses acetyl-CoA to a diverse group of short-chain ...

journal_title：Applied and environmental microbiology

authors： McMahon MD,Prather KL

更新日期：2014-02-01 00:00:00

abstract：:Exopolysaccharide-producing Lactobacillus casei CG11 was isolated from soft, white, homemade cheese. In basal minimal medium, it produces a neutral heteropolysaccharide consisting predominantly of glucose (about 75%) and rhamnose (about 15%). Plasmid curing experiments revealed that exopolysaccharide production by str...

journal_title：Applied and environmental microbiology

authors： Kojic M,Vujcic M,Banina A,Cocconcelli P,Cerning J,Topisirovic L

更新日期：1992-12-01 00:00:00

abstract：:Pili (fimbriae) were observed on cells of each of the five strains of Bradyrhizobium japonicum and the one strain of Rhizobium trifolii examined. Pili on B. japonicum were about 4 nm in diameter and polarly expressed. Piliated cells were estimated by transmission electron microscopy and hydrophobic attachment to polys...

journal_title：Applied and environmental microbiology

authors： Vesper SJ,Bauer WD

更新日期：1986-07-01 00:00:00

abstract：:Iron-oxidizing thiobacilli were adapted to grow on a chalcopyrite and a galena ore concentrate. When grown on the chalcopyrite concentrate, the bacteria exhibited a doubling time of 38.4 +/- 2.9 h, with a final cellular protein concentration of 185 mug/ml and solubilization of 10.3 g of copper per liter. When grown on...

journal_title：Applied and environmental microbiology

authors： Kingma JG,Silver M

更新日期：1980-03-01 00:00:00

abstract：:Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests th...

journal_title：Applied and environmental microbiology

authors： Zozaya-Hinchliffe M,Martin DH,Ferris MJ

更新日期：2008-03-01 00:00:00

abstract：:Campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in Sydney, Australia. Three of four flocks examined carried C. jejuni. Eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes. ...

journal_title：Applied and environmental microbiology

authors： Shanker S,Rosenfield JA,Davey GR,Sorrell TC

更新日期：1982-05-01 00:00:00

abstract：:The growth kinetics of Flavobacterium sp. strain S12 specialized in the utilization of glycerol, and a number of oligo- and polysaccharides were determined in batch-culture experiments at 15 degrees C in pasteurized tap water supplied with very low amounts of substrates. Kss for the growth on maltotriose, maltotetraos...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Hijnen WA

更新日期：1985-04-01 00:00:00

abstract：:Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while...

journal_title：Applied and environmental microbiology

authors： Deng MQ,Cliver DO

更新日期：1998-05-01 00:00:00

abstract：:Higher yields of sterigmatocystin were obtained with Aspergillus versicolor than with Bipolaris sorokiniana both in liquid and on solid media. The optimum temperature for sterigmatocystin production by A. versicolor was 27 to 29 degrees C and 23 degrees C for B. sorokiniana. In liquid shake cultures, production of ste...

journal_title：Applied and environmental microbiology

authors： Rabie CJ,Lubben A,Steyn M

更新日期：1976-08-01 00:00:00