Phytic Acid and Sodium Chloride Show Marked Synergistic Bactericidal Effects against Nonadapted and Acid-Adapted Escherichia coli O157:H7 Strains.

Abstract:

:The synergistic antimicrobial effects of phytic acid (PA), a natural extract from rice bran, plus sodium chloride against Escherichia coli O157:H7 were examined. Exposure to NaCl alone at concentrations up to 36% (wt/wt) for 5 min did not reduce bacterial populations. The bactericidal effects of PA alone were much greater than those of other organic acids (acetic, citric, lactic, and malic acids) under the same experimental conditions (P < 0.05). Combining PA and NaCl under conditions that yielded negligible effects when each was used alone led to marked synergistic effects. For example, whereas 0.4% PA or 3 or 4% NaCl alone had little or no effect on cell viability, combining the two completely inactivated both nonadapted and acid-adapted cells, reducing their numbers to unrecoverable levels (>7-log CFU/ml reduction). Flow cytometry confirmed that PA disrupted the cell membrane to a greater extent than did other organic acids, although the cells remained viable. The combination of PA and NaCl induced complete disintegration of the cell membrane. By comparison, none of the other organic acids acted synergistically with NaCl, and neither did NaCl-HCl solutions at the same pH values as the test solutions of PA plus NaCl. These results suggest that PA has great potential as an effective bacterial membrane-permeabilizing agent, and we show that the combination is a promising alternative to conventional chemical disinfectants. These findings provide new insight into the utility of natural compounds as novel antimicrobial agents and increase our understanding of the mechanisms underlying the antibacterial activity of PA.

journal_name

Appl Environ Microbiol

authors

Kim NH,Rhee MS

doi

10.1128/AEM.03307-15

subject

Has Abstract

pub_date

2015-12-04 00:00:00

pages

1040-1049

issue

4

eissn

0099-2240

issn

1098-5336

pii

AEM.03307-15

journal_volume

82

pub_type

杂志文章
  • Kinetics of methyl t-butyl ether cometabolism at low concentrations by pure cultures of butane-degrading bacteria.

    abstract::Butane-oxidizing Arthrobacter (ATCC 27778) bacteria were shown to degrade low concentrations of methyl t-butyl ether (MTBE; range, 100 to 800 microg/liter) with an apparent half-saturation concentration (K(s)) of 2.14 mg/liter and a maximum substrate utilization rate (k(c)) of 0.43 mg/mg of total suspended solids per ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.5.2197-2201.2001

    authors: Liu CY,Speitel GE Jr,Georgiou G

    更新日期:2001-05-01 00:00:00

  • Degradation of ciprofloxacin by basidiomycetes and identification of metabolites generated by the brown rot fungus Gloeophyllum striatum.

    abstract::Ciprofloxacin (CIP), a fluoroquinolone antibacterial drug, is widely used in the treatment of serious infections in humans. Its degradation by basidiomycetous fungi was studied by monitoring 14CO2 production from [14C]CIP in liquid cultures. Sixteen species inhabiting wood, soil, humus, or animal dung produced up to 3...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.4.1556-1563.1999

    authors: Wetzstein HG,Stadler M,Tichy HV,Dalhoff A,Karl W

    更新日期:1999-04-01 00:00:00

  • Effects of Glucose Concentrations on Cadmium, Copper, Mercury, and Zinc Toxicity to a Klebsiella sp.

    abstract::The influence of glucose concentration on Cd, Cu, Hg, and Zn toxicity to a Klebsiella sp. was studied by following the degradation of C-labeled glucose at pH 6.0. Uptake of C into the cells was also determined. The carbon concentrations ranged from 0.01 to 40 mg liter, which are equivalent to soluble C concentrations ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.7.1689-1693.1988

    authors: Brynhildsen L,Lundgren BV,Allard B,Rosswall T

    更新日期:1988-07-01 00:00:00

  • Predictive models for Escherichia coli concentrations at inland lake beaches and relationship of model variables to pathogen detection.

    abstract::Predictive models, based on environmental and water quality variables, have been used to improve the timeliness and accuracy of recreational water quality assessments, but their effectiveness has not been studied in inland waters. Sampling at eight inland recreational lakes in Ohio was done in order to investigate usi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02995-12

    authors: Francy DS,Stelzer EA,Duris JW,Brady AM,Harrison JH,Johnson HE,Ware MW

    更新日期:2013-03-01 00:00:00

  • Purification and characterization of an extracellular beta-1,4-mannanase from a marine bacterium, Vibrio sp. strain MA-138.

    abstract::A beta-mannanase (EC 3.2.1.78) from Vibrio sp. strain MA-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. The final ion-exchange chromatography Mono Q yielded one major active fraction and three minor acti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.12.4454-4458.1995

    authors: Tamaru Y,Araki T,Amagoi H,Mori H,Morishita T

    更新日期:1995-12-01 00:00:00

  • Propachlor removal by Pseudomonas strain GCH1 in an immobilized-cell system.

    abstract::A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.3.1190-1194.2000

    authors: Martín M,Mengs G,Plaza E,Garbi C,Sánchez M,Gibello A,Gutierrez F,Ferrer E

    更新日期:2000-03-01 00:00:00

  • Recombineering using RecTE from Pseudomonas syringae.

    abstract::In this report, we describe the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecET prote...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00911-10

    authors: Swingle B,Bao Z,Markel E,Chambers A,Cartinhour S

    更新日期:2010-08-01 00:00:00

  • Thermal and nonthermal effects of discontinuous microwave exposure (2.45 gigahertz) on the cell membrane of Escherichia coli.

    abstract::The aim of this study was to investigate the effects on the cell membranes of Escherichia coli of 2.45-GHz microwave (MW) treatment under various conditions with an average temperature of the cell suspension maintained at 37°C in order to examine the possible thermal versus nonthermal effects of short-duration MW expo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00789-14

    authors: Rougier C,Prorot A,Chazal P,Leveque P,Leprat P

    更新日期:2014-08-01 00:00:00

  • Linked redox precipitation of sulfur and selenium under anaerobic conditions by sulfate-reducing bacterial biofilms.

    abstract::A biofilm-forming strain of sulfate-reducing bacteria (SRB), isolated from a naturally occurring mixed biofilm and identified by 16S rDNA analysis as a strain of Desulfomicrobium norvegicum, rapidly removed 200 micro M selenite from solution during growth on lactate and sulfate. Elemental selenium and elemental sulfur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.12.7063-7072.2003

    authors: Hockin SL,Gadd GM

    更新日期:2003-12-01 00:00:00

  • Low-temperature decontamination with hydrogen peroxide or chlorine dioxide for space applications.

    abstract::The currently used microbial decontamination method for spacecraft and components uses dry-heat microbial reduction at temperatures of >110°C for extended periods to prevent the contamination of extraplanetary destinations. This process is effective and reproducible, but it is also long and costly and precludes the us...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07948-11

    authors: Pottage T,Macken S,Giri K,Walker JT,Bennett AM

    更新日期:2012-06-01 00:00:00

  • New Selective Growth Inhibitors of Sphaerotilus natans, Anslimins A and B.

    abstract::Selective growth inhibitors of Sphaerotilus natans were detected in the culture broths of several strains of Streptomyces. Anslimins A and B were isolated from the culture broth of Streptomyces oganonensis by adsorption on resinous adsorbent followed by elution with 50% aqueous acetone. The A and B components were the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.6.1123-1128.1980

    authors: Takiguchi Y,Yoshikawa H,Terao M

    更新日期:1980-06-01 00:00:00

  • Precipitation of barite by Myxococcus xanthus: possible implications for the biogeochemical cycle of barium.

    abstract::Bacterial precipitation of barite (BaSO(4)) under laboratory conditions is reported for the first time. The bacterium Myxococcus xanthus was cultivated in a solid medium with a diluted solution of barium chloride. Crystallization occurred as a result of the presence of live bacteria and the bacterial metabolic activit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.9.5722-5725.2003

    authors: González-Muñoz MT,Fernández-Luque B,Martínez-Ruiz F,Ben Chekroun K,Arias JM,Rodríguez-Gallego M,Martínez-Cañamero M,de Linares C,Paytan A

    更新日期:2003-09-01 00:00:00

  • Improved inactivation of nonenveloped enteric viruses and their surrogates by a novel alcohol-based hand sanitizer.

    abstract::Norovirus is the leading cause of food-related illness in the United States, and contamination of ready-to-eat items by food handlers poses a high risk for disease. This study reports the in vitro (suspension test) and in vivo (fingerpad protocol) assessments of a new ethanol-based hand sanitizer containing a synergis...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00487-08

    authors: Macinga DR,Sattar SA,Jaykus LA,Arbogast JW

    更新日期:2008-08-01 00:00:00

  • Dynamics of ruminal ciliated protozoa in feedlot cattle.

    abstract::Fluctuations in ciliated protozoan concentrations were monitored in 40 individually fed crossbred heifers that were stepped up to an 85% concentrate diet either slowly (12 days) or rapidly (3 days), with or without monensin (30 ppm). Ruminal fluid was withdrawn from all animals by stomach tube at the start of the stud...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.10.3174-3178.1990

    authors: Towne G,Nagaraja TG,Brandt RT Jr,Kemp KE

    更新日期:1990-10-01 00:00:00

  • Clinically Unreported Salmonellosis Outbreak Detected via Comparative Genomic Analysis of Municipal Wastewater Salmonella Isolates.

    abstract::Municipal wastewater includes human waste that contains both commensal and pathogenic enteric microorganisms, and this collective community microbiome can be monitored for community diseases. In a previous study, we assessed the salmonellosis disease burden using municipal wastewater from Honolulu, Hawaii, which was m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00139-19

    authors: Diemert S,Yan T

    更新日期:2019-05-02 00:00:00

  • Selection of Streptomyces ambofaciens mutants that produce large quantities of spiramycin and determination of optimal conditions for spiramycin production.

    abstract::The aim of this work was to develop a strategy to isolate a morphologically stable mutant of Streptomyces ambofaciens ATCC 15154 which produced high titers of spiramycin. The rationale was to grow a nitrosoguanidine-mutated population for many generations under nonselective conditions followed by two cycles of protopl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.11.3511-3514.1990

    authors: Ford LM,Eaton TE,Godfrey OW

    更新日期:1990-11-01 00:00:00

  • Probing the functional diversity of global pristine soil communities with 3-chlorobenzoate reveals that communities of generalists dominate catabolic transformation.

    abstract::Understanding of functional diversity of microbial populations has lagged description of their molecular diversity. Differences in substrate specificity, kinetics, products, and regulation can dramatically influence phenotypic variation among closely related strains, features that are missed when the strains studied a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01905-13

    authors: Rhodes AN,Fulthorpe RR,Tiedje JM

    更新日期:2013-11-01 00:00:00

  • Silage collected from dairy farms harbors an abundance of listeriaphages with considerable host range and genome size diversity.

    abstract::Since the food-borne pathogen Listeria monocytogenes is common in dairy farm environments, it is likely that phages infecting this bacterium ("listeriaphages") are abundant on dairy farms. To better understand the ecology and diversity of listeriaphages on dairy farms and to develop a diverse phage collection for furt...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01859-12

    authors: Vongkamjan K,Switt AM,den Bakker HC,Fortes ED,Wiedmann M

    更新日期:2012-12-01 00:00:00

  • Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilms.

    abstract::Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experience...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.5.1690-1692.1994

    authors: Stewart PS,Griebe T,Srinivasan R,Chen CI,Yu FP,deBeer D,McFeters GA

    更新日期:1994-05-01 00:00:00

  • Degradation of Coal by the Fungi Polyporus versicolor and Poria monticola.

    abstract::We report that two species of basidiomycete fungi (Polyporus versicolor and Poria monticola) grow in minimal liquid or solid medium when supplemented with crushed lignite coal. The fungi also grow directly on crushed lignite coal. The growth of both fungi was observed qualitatively as the production and extension of h...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.1.23-27.1982

    authors: Cohen MS,Gabriele PD

    更新日期:1982-07-01 00:00:00

  • Pyruvate and lactate metabolism by Shewanella oneidensis MR-1 under fermentation, oxygen limitation, and fumarate respiration conditions.

    abstract::Shewanella oneidensis MR-1 is a facultative anaerobe that derives energy by coupling organic matter oxidation to the reduction of a wide range of electron acceptors. Here, we quantitatively assessed the lactate and pyruvate metabolism of MR-1 under three distinct conditions: electron acceptor-limited growth on lactate...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05382-11

    authors: Pinchuk GE,Geydebrekht OV,Hill EA,Reed JL,Konopka AE,Beliaev AS,Fredrickson JK

    更新日期:2011-12-01 00:00:00

  • Growth of geobacter sulfurreducens with acetate in syntrophic cooperation with hydrogen-oxidizing anaerobic partners

    abstract::Pure cultures of Geobacter sulfurreducens and other Fe(III)-reducing bacteria accumulated hydrogen to partial pressures of 5 to 70 Pa with acetate, butyrate, benzoate, ethanol, lactate, or glucose as the electron donor if electron release to an acceptor was limiting. G. sulfurreducens coupled acetate oxidation with el...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.6.2232-2236.1998

    authors: Cord-Ruwisch R,Lovley DR,Schink B

    更新日期:1998-06-01 00:00:00

  • Production of trypsin inhibitor by a Cephalosporium sp.

    abstract::Conditions for the production of the trypsin inhibitor from Cephalosporium sp. KM 388 were investigated. Polypeptone-meat extract-glucose medium supported excellent production of the trypsin inhibitor. In this medium, polypeptone and meat extract were utilized both as carbon and nitrogen sources and as limiting substr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.4.631-635.1978

    authors: Tsuchiya K,Kimura T

    更新日期:1978-04-01 00:00:00

  • Mechanisms of bactericidal action of cinnamaldehyde against Listeria monocytogenes and of eugenol against L. monocytogenes and Lactobacillus sakei.

    abstract::The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.5750-5755.2004

    authors: Gill AO,Holley RA

    更新日期:2004-10-01 00:00:00

  • AmyA, an alpha-amylase with beta-cyclodextrin-forming activity, and AmyB from the thermoalkaliphilic organism Anaerobranca gottschalkii: two alpha-amylases adapted to their different cellular localizations.

    abstract::Two alpha-amylase genes from the thermophilic alkaliphile Anaerobranca gottschalkii were cloned, and the corresponding enzymes, AmyA and AmyB, were investigated after purification of the recombinant proteins. Based on their amino acid sequences, AmyA is proposed to be a lipoprotein with extracellular localization and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.7.3709-3715.2005

    authors: Ballschmiter M,Armbrecht M,Ivanova K,Antranikian G,Liebl W

    更新日期:2005-07-01 00:00:00

  • Insecticidal Activity of the Toxins from Bacillus thuringiensis subspecies kurstaki and tenebrionis Adsorbed and Bound on Pure and Soil Clays.

    abstract::The release of transgenic plants and microorganisms expressing truncated genes from various subspecies of Bacillus thuringiensis that encode active insecticidal toxins rather than inactive protoxins could result in the accumulation of these active proteins in soil, especially when bound on clays and other soil particl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.5.1786-1790.1995

    authors: Tapp H,Stotzky G

    更新日期:1995-05-01 00:00:00

  • Survival in soil of different toluene-degrading Pseudomonas strains after solvent shock.

    abstract::We assayed the tolerance to solvents of three toluene-degrading Pseudomonas putida strains and Pseudomonas mendocina KR1 in liquid and soil systems. P. putida DOT-T1 tolerated concentrations of heptane, propylbenzene, octanol, and toluene of at least 10% (vol/vol), while P. putida F1 and EEZ15 grew well in the presenc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.1.38-42.1998

    authors: Huertas MJ,Duque E,Marqués S,Ramos JL

    更新日期:1998-01-01 00:00:00

  • Increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle.

    abstract::Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by us...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.9.5685-5687.2004

    authors: De Oliveira VC,Maeda I,Delessert S,Poirier Y

    更新日期:2004-09-01 00:00:00

  • Nuclear Ribosomal DNA as a Probe for Genetic Variability in the Japanese Pear Pathotype of Alternaria alternata.

    abstract::A restriction fragment length polymorphism analysis of nuclear ribosomal RNA genes (rDNA) was used to measure the amount and distribution of genetic variability in populations of the Japanese pear pathotype of Alternaria alternata on both micro- and macrogeographical scales. A total of 322 isolates were obtained from ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.10.3197-3205.1993

    authors: Adachi Y,Watanabe H,Tanabe K,Doke N,Nishimura S,Tsuge T

    更新日期:1993-10-01 00:00:00

  • Inducible promoter-repressor system from the Lactobacillus casei phage phiFSW.

    abstract::With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless bet...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.8.4132-4135.2002

    authors: Binishofer B,Moll I,Henrich B,Bläsi U

    更新日期:2002-08-01 00:00:00