Use of Two-Dimensional Polyacrylamide Electrophoresis to Demonstrate that Putative Rhizobium Cross-Inoculation Mutants Actually Are Contaminants.

abstract：:The survival of Azospirillum brasilense Cd and Sp-245 in the rhizosphere of wheat and tomato plants and in 23 types of plant-free sterilized soils obtained from a wide range of environments in Israel and Mexico was evaluated. Large numbers of A. brasilense cells were detected in all the rhizospheres tested, regardless...

journal_title：Applied and environmental microbiology

authors： Bashan Y,Puente ME,Rodriguez-Mendoza MN,Toledo G,Holguin G,Ferrera-Cerrato R,Pedrin S

更新日期：1995-05-01 00:00:00

abstract：:When 7-aminocephalosporanic acid (7-ACA) was used as a single carbon source in the enrichment culture medium for screening 7-ACA-degrading microorganisms, pink yeast colonies appeared frequently, and these were identified as Rhodotorula glutinis. These intact R. glutinis cells converted (i) 7-ACA to deacetyl-7-ACA (7-...

journal_title：Applied and environmental microbiology

authors： Sakai Y,Abe T,Ohbayashi Y,Isaka K,Yamamoto K,Tani Y,Kato N

更新日期：1996-07-01 00:00:00

abstract：:Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscil...

journal_title：Applied and environmental microbiology

authors： Hallez R,Letesson JJ,Vandenhaute J,De Bolle X

更新日期：2007-02-01 00:00:00

abstract：:An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity. The highly l-enantioselective enzyme resembled thiol reagent-sensitive alkaline serine proteinases and was strongly activated by divalent cations. It possessed a h...

journal_title：Applied and environmental microbiology

authors： Hermes HF,Sonke T,Peters PJ,van Balken JA,Kamphuis J,Dijkhuizen L,Meijer EM

更新日期：1993-12-01 00:00:00

abstract：:Biofilms were grown from wild-type (WT) Pseudomonas aeruginosa PAO1 and the cell signaling lasI mutant PAO1-JP1 under laminar and turbulent flows to investigate the relative contributions of hydrodynamics and cell signaling for biofilm formation. Various biofilm morphological parameters were quantified using Image Str...

journal_title：Applied and environmental microbiology

authors： Purevdorj B,Costerton JW,Stoodley P

更新日期：2002-09-01 00:00:00

abstract：:l-Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) from Rhodotorula rubra has been used in the commercial manufacture of l-phenylalanine from trans-cinnamic acid. In this study, R. graminis PAL was investigated. Mutant strain GX6000 was isolated after ethyl methanesulfonate mutagenesis of wild-type R. graminis GX5007 by ...

journal_title：Applied and environmental microbiology

authors： Orndorff SA,Costantino N,Stewart D,Durham DR

更新日期：1988-04-01 00:00:00

abstract：:The rapid degradation of marine infrastructure at the low tide level due to accelerated low-water corrosion (ALWC) is a problem encountered worldwide. Despite this, there is limited understanding of the microbial communities involved in this process. We obtained samples of the orange-colored tubercles commonly associa...

journal_title：Applied and environmental microbiology

authors： Phan HC,Wade SA,Blackall LL

更新日期：2020-06-17 00:00:00

abstract：:We determined the prevalences of Escherichia coli O157:H7 in feces, hide, and carcasses of meat goats at a U.S. processing plant. Prevalences were 11.1%, 2.7%, and 2.7%, respectively. Sixteen pulsed-field gel electrophoresis (PFGE) subtypes were identified among 49 E. coli O157:H7 isolates, some of which were present ...

journal_title：Applied and environmental microbiology

authors： Jacob ME,Foster DM,Rogers AT,Balcomb CC,Sanderson MW

更新日期：2013-07-01 00:00:00

abstract：:The design of fully or partly defined media for mycoplasma cultivation involves the need to provide the essential lipids, cholesterol and long-chain fatty acids, in an assimilable and nontoxic form. This study introduces cyclodextrins (CDs) as carriers of these lipids, thus suggesting alternatives to serum or bovine s...

journal_title：Applied and environmental microbiology

authors： Greenberg-Ofrath N,Terespolosky Y,Kahane I,Bar R

更新日期：1993-02-01 00:00:00

abstract：:Trench leachate samples collected anoxically from shallow-land, low-level radioactive waste disposal sites were analyzed for total aerobic and anaerobic populations, sulfate reducers, denitrifiers, and methanogens. Among the several aerobic and anaerobic bacteria isolated, only Bacillus sp., Pseudomonas sp., Citrobact...

journal_title：Applied and environmental microbiology

authors： Francis AJ,Dobbs S,Nine BJ

更新日期：1980-07-01 00:00:00

abstract：:Newcastle disease, one of the most important health problems that affects the poultry industry around the world, is caused by virulent strains of Newcastle disease virus. Newcastle disease virus is considered to be endemic in several countries in the Americas, including Mexico. In order to control Newcastle disease ou...

journal_title：Applied and environmental microbiology

authors： Cardenas Garcia S,Navarro Lopez R,Morales R,Olvera MA,Marquez MA,Merino R,Miller PJ,Afonso CL

更新日期：2013-08-01 00:00:00

abstract：:The beta-subgroup of the Proteobacteria has been shown to be important in aquatic habitats and was investigated in depth here by molecular 16S rRNA techniques in biofilms of the Elbe River and its polluted tributary, the Spittelwasser River. The bacterial 16S rRNA genes were cloned from each site, screened for beta-pr...

journal_title：Applied and environmental microbiology

authors： Brümmer IH,Felske A,Wagner-Döbler I

更新日期：2003-08-01 00:00:00

abstract：:Preinfection development in Colletotrichum spp. exhibits three morphologies (conidia, germ tubes, and appressoria) and is directed by a complex interplay of environmental signals. Germ tube morphogenesis for Colletotrichum trifolii and the related fungus Colletotrichum gloeosporioides f. sp. aeschynomene was shown to ...

journal_title：Applied and environmental microbiology

authors： Buhr TL,Dickman MB

更新日期：1997-06-01 00:00:00

abstract：:Escherichia coli O157:H7 carried on plant surfaces, including alfalfa sprouts, has been implicated in food poisoning and outbreaks of disease in the United States. Adhesion to cell surfaces is a key component for bacterial establishment and colonization on many types of surfaces. Several E. coli O157:H7 surface protei...

journal_title：Applied and environmental microbiology

authors： Torres AG,Jeter C,Langley W,Matthysse AG

更新日期：2005-12-01 00:00:00

abstract：:Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F(70)(10) of 1, 3, and 5 min), ...

journal_title：Applied and environmental microbiology

authors： Seyer K,Lessard M,Piette G,Lacroix M,Saucier L

更新日期：2003-06-01 00:00:00

abstract：:We compared the influences of grazing by the bacterivorous nanoflagellate Poterioochromonas sp. strain DS on ultramicrobacterial Actinobacteria affiliated with the Luna-2 cluster and ultramicrobacterial Betaproteobacteria of the species Polynucleobacter cosmopolitanus. These bacteria were almost identical in size (<0....

journal_title：Applied and environmental microbiology

authors： Tarao M,Jezbera J,Hahn MW

更新日期：2009-07-01 00:00:00

abstract：:Upflow, anaerobic, fixed-bed, activated charcoal biotreatment columns capable of operating at free cyanide concentrations of greater than 100 mg liter-1 with a hydraulic retention time of less than 48 h were developed. Methanogenesis was maintained under a variety of feed medium conditions which included ethanol, phen...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Cooper DA,Speece R,Henson M

更新日期：1991-06-01 00:00:00

abstract：:Small streams and their headwaters are key sources of microbial diversity in fluvial systems and serve as an entry point for bacteria from surrounding environments. Community assembly processes occurring in these streams shape downstream population structure and nutrient cycles. To elucidate the development and stabil...

journal_title：Applied and environmental microbiology

authors： Teachey ME,McDonald JM,Ottesen EA

更新日期：2019-05-16 00:00:00

abstract：:Antigens from the methicillin-resistant Staphylococcus aureus (MRSA) cell wall have been shown to be immunogenic in vivo and upregulated during biofilm growth. In this study, we created purified, recombinant forms of selected antigens and biofilm-upregulated, cell wall-associated proteins. These proteins were shown to...

journal_title：Applied and environmental microbiology

authors： Brady RA,Leid JG,Kofonow J,Costerton JW,Shirtliff ME

更新日期：2007-10-01 00:00:00

abstract：:The Sinorhizobium meliloti ORFeome project cloned 6,314 open reading frames (ORFs) into a modified Gateway entry vector system from which the ORFs could be transferred to destination vectors in vivo via bacterial conjugation. In this work, a reporter gene destination vector, pMK2030, was constructed and used to genera...

journal_title：Applied and environmental microbiology

authors： Humann JL,Schroeder BK,Mortimer MW,House BL,Yurgel SN,Maloney SC,Ward KL,Fallquist HM,Ziemkiewicz HT,Kahn ML

更新日期：2008-11-01 00:00:00

abstract：:Typing of F-specific RNA (FRNA) coliphages has been proposed as a useful method for distinguishing human from animal fecal contamination in environmental samples. Group II and III FRNA coliphages are generally associated with human wastes, but several exceptions have been noted. In the present study, we have genotyped...

journal_title：Applied and environmental microbiology

authors： Stewart JR,Vinjé J,Oudejans SJ,Scott GI,Sobsey MD

更新日期：2006-02-01 00:00:00

abstract：:In natural environments such as anaerobic digesters, bacteria are frequently subjected to the stress of nutrient fluxes because of the continual changes in the flow of nutrients, and to survive, they must be capable of adapting readily to nutrient changes. In this study, the metabolic activities of Escherichia coli, S...

journal_title：Applied and environmental microbiology

authors： Kearney TE,Larkin MJ,Levett PN

更新日期：1994-10-01 00:00:00

abstract：:The bacterial communities associated with the development of necroses in injured agria cactus tissue were examined in the field by using both human-induced injuries and cactus tissue inoculated with cactophilic bacteria. Whole-cell bacterial fatty acids were used to determine when and where each of 23 detected species...

journal_title：Applied and environmental microbiology

authors： Foster JL,Fogleman JC

更新日期：1994-02-01 00:00:00

abstract：:Food-borne hepatitis A outbreaks may be prevented by subjecting foods at risk of virus contamination to moderate treatments of high hydrostatic pressure (HHP). A pretreatment promoting hepatitis A virus (HAV) capsid-folding changes enhances the virucidal effect of HHP, indicating that its efficacy depends on capsid co...

journal_title：Applied and environmental microbiology

authors： D'Andrea L,Pérez-Rodríguez FJ,Costafreda MI,Beguiristain N,Fuentes C,Aymerich T,Guix S,Bosch A,Pintó RM

更新日期：2014-10-01 00:00:00

abstract：:Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests th...

journal_title：Applied and environmental microbiology

authors： Zozaya-Hinchliffe M,Martin DH,Ferris MJ

更新日期：2008-03-01 00:00:00

abstract：:The lytic action of glycerol and sucrose esters of fatty acids with different carbon chain lengths on the exponentially growing cells of Bacillus subtilis 168 was investigated. Of each series of esters, glycerol dodecanoate and sucrose hexadecanoate were the most active. Lysis at 1 h after the addition of 0.1 mM glyce...

journal_title：Applied and environmental microbiology

authors： Tsuchido T,Ahn YH,Takano M

更新日期：1987-03-01 00:00:00

abstract：:The aromatic amino acids are synthesized via a common biosynthetic pathway. A tryptophan-producing mutant of Corynebacterium glutamicum was genetically engineered to produce tyrosine or phenylalanine in abundance. To achieve this, three biosynthetic genes encoding the first enzyme in the common pathway, 3-deoxy-d-arab...

journal_title：Applied and environmental microbiology

authors： Ikeda M,Katsumata R

更新日期：1992-03-01 00:00:00

abstract：:Mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (VOC) by gas chromatography-mass spectrometry (GC/MS). Fifty mesophilic and twenty-nine ...

journal_title：Applied and environmental microbiology

authors： Ercolini D,Russo F,Nasi A,Ferranti P,Villani F

更新日期：2009-04-01 00:00:00

abstract：:Many fungal pathogens have short generation times, large population sizes, and mixed reproductive systems, providing high potential to adapt to heterogeneous environments of agroecosystems. Such adaptation complicates disease management and threatens food production. A better understanding of pathogen population biolo...

journal_title：Applied and environmental microbiology

authors： Kozhar O,Larsen MM,Grünwald NJ,Peever TL

更新日期：2020-04-17 00:00:00

abstract：:Washed cell suspensions of Desulfovibrio vulgaris rapidly reduced Cr(VI) to Cr(III) with H(2) as the electron donor. The c(3) cytochrome from this organism functioned as a Cr(VI) reductase. D. vulgaris may have advantages over previously described Cr(VI) reducers for the bioremediation of Cr(VI)-contaminated waters. ...

journal_title：Applied and environmental microbiology

authors： Lovley DR,Phillips EJ

更新日期：1994-02-01 00:00:00