Sequence variation among group III F-specific RNA coliphages from water samples and swine lagoons.

abstract：:In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fos...

journal_title：Applied and environmental microbiology

authors： Jogler C,Lin W,Meyerdierks A,Kube M,Katzmann E,Flies C,Pan Y,Amann R,Reinhardt R,Schüler D

更新日期：2009-06-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:A total of 113 strains of mesophilic strains lactic streptococci of the species Streptococcus lactis, S. lactis subsp. diacetilactis, and S. cremoris, chosen from 291 strains that had been previously classified into six groups on the basis of their sensitivity to 132 virulent phages, were subjected to induction with m...

journal_title：Applied and environmental microbiology

authors： Reyrolle J,Chopin MC,Letellier F,Novel G

更新日期：1982-02-01 00:00:00

abstract：:Biotin-labeled DNA probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (Lactobacillus delbrueckii 21 and Lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. The forestomach sections were incubated ...

journal_title：Applied and environmental microbiology

authors： Tannock GW

更新日期：1989-02-01 00:00:00

abstract：:A beta-mannanase (EC 3.2.1.78) from Vibrio sp. strain MA-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. The final ion-exchange chromatography Mono Q yielded one major active fraction and three minor acti...

journal_title：Applied and environmental microbiology

authors： Tamaru Y,Araki T,Amagoi H,Mori H,Morishita T

更新日期：1995-12-01 00:00:00

abstract：:Deletion of chromosomally inserted gene sequences from Clavibacter xyli subsp. cynodontis, a xylem-inhabiting endophyte, was studied in vitro and in planta. We found that nonreplicating plasmid pCG610, which conferred resistance to kanamycin and tetracycline and contained segments of C. xyli subsp. cynodontis genomic ...

journal_title：Applied and environmental microbiology

authors： Turner JT,Lampel JS,Stearman RS,Sundin GW,Gunyuzlu P,Anderson JJ

更新日期：1991-12-01 00:00:00

abstract：:A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The Km and Vmax values for the substrate dihydrox...

journal_title：Applied and environmental microbiology

authors： Huang K,Rudolph FB,Bennett GN

更新日期：1999-07-01 00:00:00

abstract：:Vibrio vulnificus biotype 2 is a primary pathogen for eels and, as has recently been suggested, an opportunistic pathogen for humans. In this study we have investigated the ability of V. vulnificus biotype 2 to obtain iron by siderophore-mediated mechanisms and evaluated the importance of free iron in vibriosis. The v...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Fouz B,Alcaide E,Amaro C

更新日期：1996-03-01 00:00:00

abstract：:A number of hydrothermal vent sites exist on the summit of the Loihi Seamount, a shield volcano that is part of the Hawaiian archipelago. The vents are 1,100 to 1,325 m below the surface and range in temperature from slightly above ambient (10 degrees C) to high temperature (167 degrees C). The vent fluid is character...

journal_title：Applied and environmental microbiology

authors： Emerson D,Moyer CL

更新日期：2002-06-01 00:00:00

abstract：:During the course of immunologic studies involving the gastrointestinal colonization of mice with Candida albicans, it became apparent that the animals were being exposed to large numbers of Aspergillus fumigatus spores which interfered with the C. albicans colonization. To determine the source of the A. fumigatus exp...

journal_title：Applied and environmental microbiology

authors： Mayeux P,Dupepe L,Dunn K,Balsamo J,Domer J

更新日期：1995-06-01 00:00:00

abstract：:Arctic ground squirrels (Urocitellus parryii) are active for a scant 3 to 5 months of the year. During the active season, adult squirrels compete for mates, reproduce, and fatten in preparation for hibernation, while juvenile squirrels, weaned in early July, must grow and acquire sufficient fat to survive their first ...

journal_title：Applied and environmental microbiology

authors： Stevenson TJ,Buck CL,Duddleston KN

更新日期：2014-07-01 00:00:00

abstract：:Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg) and two mutants with enhanced agglutinability (Agg) possessed...

journal_title：Applied and environmental microbiology

authors： Anderson AJ,Habibzadegah-Tari P,Tepper CS

更新日期：1988-02-01 00:00:00

abstract：:During a molecular phylogenetic survey of extremely acidic (pH < 1), metal-rich acid mine drainage habitats in the Richmond Mine at Iron Mountain, Calif., we detected 16S rRNA gene sequences of a novel bacterial group belonging to the order Rickettsiales in the Alphaproteobacteria. The closest known relatives of this ...

journal_title：Applied and environmental microbiology

authors： Baker BJ,Hugenholtz P,Dawson SC,Banfield JF

更新日期：2003-09-01 00:00:00

abstract：:Profiles of dissolved O(2) and methane with increasing depth were generated for Lake Washington sediment, which suggested the zone of methane oxidation is limited to the top 0.8 cm of the sediment. Methane oxidation potentials were measured for 0.5-cm layers down to 1.5 cm and found to be relatively constant at 270 to...

journal_title：Applied and environmental microbiology

authors： Auman AJ,Stolyar S,Costello AM,Lidstrom ME

更新日期：2000-12-01 00:00:00

abstract：:Previously, members of our group reported the isolation and characterization of mutacin II from Streptococcus mutans T8 and the genetic analyses of the mutacin II biosynthesis genes (J. Novak, P. W. Caufield, and E. J. Miller, J. Bacteriol. 176:4316-4320, 1994; F. Qi, P. Chen, and P. W. Caufield, Appl. Environ. Microb...

journal_title：Applied and environmental microbiology

authors： Qi F,Chen P,Caufield PW

更新日期：1999-09-01 00:00:00

abstract：:The kinetics of oxygen reduction by Desulfovibrio salexigens Mast1 and the role of polyglucose in this activity were examined and compared with those of strains of D. desulfuricans and D. gigas. Oxidation rates were highest at air saturation (up to 40 nmol of O(2) min mg of protein) and declined with decreasing oxygen...

journal_title：Applied and environmental microbiology

authors： van Niel EW,Gottschal JC

更新日期：1998-03-01 00:00:00

abstract：:The cloned 135-kDa CryIC delta-endotoxin from Bacillus thuringiensis is a lepidopteran-active toxin, displaying high activity in vivo against Spodoptera litoralis and Spodoptera frugiperda larvae and in vitro against the S. frugiperda Sf9 cell line. Here, we report that the CryIC delta-endotoxin cloned from B. thuring...

journal_title：Applied and environmental microbiology

authors： Smith GP,Merrick JD,Bone EJ,Ellar DJ

更新日期：1996-02-01 00:00:00

abstract：:Conserved regions about 420 bp long of the pelADE cluster specific to Erwinia chrysanthemi were amplified by PCR and used to differentiate 78 strains of E. chrysanthemi that were obtained from different hosts and geographical areas. No PCR products were obtained from DNA samples extracted from other pectinolytic and n...

journal_title：Applied and environmental microbiology

authors： Nassar A,Darrasse A,Lemattre M,Kotoujansky A,Dervin C,Vedel R,Bertheau Y

更新日期：1996-07-01 00:00:00

abstract：:Vibrio cholerae, an environmental organism, is a facultative human pathogen. Here, we report the virulence profiles, comprising 18 genetic markers, of 102 clinical and 692 environmental V. cholerae strains isolated in Bangladesh between March 2004 and January 2006, showing the variability of virulence determinants wit...

journal_title：Applied and environmental microbiology

authors： Hasan NA,Ceccarelli D,Grim CJ,Taviani E,Choi J,Sadique A,Alam M,Siddique AK,Sack RB,Huq A,Colwell RR

更新日期：2013-09-01 00:00:00

abstract：:The phenylurea herbicide diuron [N-(3,4-dichlorophenyl)-N,N-dimethylurea] is widely used in a broad range of herbicide formulations, and consequently, it is frequently detected as a major water contaminant in areas where there is extensive use. We constructed a linuron [N-(3,4-dichlorophenyl)-N-methoxy-N-methylurea]- ...

journal_title：Applied and environmental microbiology

authors： Sørensen SR,Albers CN,Aamand J

更新日期：2008-04-01 00:00:00

abstract：:The metabolic and energetic characterization of the growth of Leuconostoc oenos on glucose-citrate or glucose-fructose mixtures enables the potential role of this bacterium in the wine-making process to be ascertained. Moreover, mixotrophic conditions remain a suitable means for improving biomass productivities of mal...

journal_title：Applied and environmental microbiology

authors： Salou P,Loubiere P,Pareilleux A

更新日期：1994-05-01 00:00:00

abstract：:The aromatic amino acids are synthesized via a common biosynthetic pathway. A tryptophan-producing mutant of Corynebacterium glutamicum was genetically engineered to produce tyrosine or phenylalanine in abundance. To achieve this, three biosynthetic genes encoding the first enzyme in the common pathway, 3-deoxy-d-arab...

journal_title：Applied and environmental microbiology

authors： Ikeda M,Katsumata R

更新日期：1992-03-01 00:00:00

abstract：:Malonyl-coenzyme A (CoA) decarboxylase, malonyl-CoA synthetase, and malonate transporter mutants of Rhizobium leguminosarum bv. viciae and trifolii fixed N2 at wild-type rates on pea and clover, respectively. Thus, malonate does not drive N2 fixation in legume nodules. ...

journal_title：Applied and environmental microbiology

authors： Karunakaran R,East AK,Poole PS

更新日期：2013-07-01 00:00:00

abstract：:The colonization of granular activated carbon columns by bacteria can have both beneficial and potentially detrimental consequences. Bacterial growth on the carbon surface can remove adsorbed organics and thus partially regenerate the carbon bed. However, growth can also increase the levels of bacteria in the column e...

journal_title：Applied and environmental microbiology

authors： Shimp RJ,Pfaender FK

更新日期：1982-08-01 00:00:00

abstract：:Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests th...

journal_title：Applied and environmental microbiology

authors： Zozaya-Hinchliffe M,Martin DH,Ferris MJ

更新日期：2008-03-01 00:00:00

abstract：:Parental strains and asporogenous mutants of Bacillus thuringiensis subspp. kurstaki and aizawai produced high yields of delta-endotoxin on M medium, which contained 330 mug of potassium per ml, but not on ST and ST-a media, each of which contained only 11 mug of potassium per ml. On ST and ST-a media, refractile gran...

journal_title：Applied and environmental microbiology

authors： Wakisaka Y,Masaki E,Nishimoto Y

更新日期：1982-06-01 00:00:00

abstract：:Differences in biological activities (hemagglutination, hemolytic, leukotoxic, and virulence) and ribotypes between the two subspecies of Fusobacterium necrophorum of bovine ruminal and liver abscess origins were investigated. Hemagglutination activity was present in all hepatic, but only some ruminal, strains of Fuso...

journal_title：Applied and environmental microbiology

authors： Okwumabua O,Tan Z,Staats J,Oberst RD,Chengappa MM,Nagaraja TG

更新日期：1996-02-01 00:00:00

abstract：:An outstanding challenge toward efficient production of biofuels and value-added chemicals from plant biomass is the impact that lignocellulose-derived inhibitors have on microbial fermentations. Elucidating the mechanisms that underlie their toxicity is critical for developing strategies to overcome them. Here, using...

journal_title：Applied and environmental microbiology

authors： Pisithkul T,Jacobson TB,O'Brien TJ,Stevenson DM,Amador-Noguez D

更新日期：2015-09-01 00:00:00

abstract：:In this study, we describe the association of three Campylobacter jejuni metabolism-related traits, γ-glutamyl-transpeptidase (GGT), fucose permease (fucP), and secreted L-asparaginase [ansB(s)], with multilocus sequence types (STs). A total of 710 C. jejuni isolates with known STs were selected and originated from hu...

journal_title：Applied and environmental microbiology

authors： de Haan CP,Llarena AK,Revez J,Hänninen ML

更新日期：2012-08-01 00:00:00

abstract：:In this article, a new mechanism influencing the transport of microorganisms through unsaturated porous media is examined, and a new method for directly visualizing bacterial behavior within a porous medium under controlled chemical and flow conditions is introduced. Resting cells of hydrophilic and relatively hydroph...

journal_title：Applied and environmental microbiology

authors： Wan J,Wilson JL,Kieft TL

更新日期：1994-02-01 00:00:00