Abstract:
:Production of propionicin PLG-1 by Propionibacterium thoenii P127 was pH dependent, with maximal activity detected in supernatants of cultures grown at pH 7.0 Propionicin PLG-1 was purified by ion-exchange chromatography and isoelectric focusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of propionicin PLG-1 purified through isoelectric focusing resolved a protein band with a molecular weight of 10,000. Propionicin PLG-1 was bactericidal to sensitive cells, demonstrating single-hit kinetics. The producing strain harbored a single plasmid (pLG1) with an approximate size of 250 kb. Preliminary data indicate that both propionicin PLG-1 and immunity to the bacteriocin are encoded on the chromosome. Exposure of strain P127 to acriflavine or to N-methyl-N'-nitro-N-nitrosoguanidine yielded isolates that no longer produced bacteriocin activity and isolates that were cured of the plasmid. However, loss of bacteriocin production was not correlated with loss of the plasmid. Isolates cured of the plasmid were phenotypically identical to plasmid-bearing cells in fermentation patterns, pigment production, and growth characteristics.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Lyon WJ,Glatz BAdoi
10.1128/AEM.59.1.83-88.1993subject
Has Abstractpub_date
1993-01-01 00:00:00pages
83-8issue
1eissn
0099-2240issn
1098-5336journal_volume
59pub_type
杂志文章abstract::It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting enviro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/AEM.41.3.752-757.1981
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doi:10.1128/AEM.40.3.678-680.1980
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1988-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.70.10.5898-5904.2004
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1976-07-01 00:00:00
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