Permanent colonization by Lactobacillus casei is hindered by the low rate of cell division in mouse gut.

abstract：:In the vast number of random mutagenesis experiments that have targeted protein thermostability, single amino acid substitutions that increase the apparent melting temperature (Tm) of the enzyme more than 1 to 2 degrees C are rare and often require the creation of a large library of mutated genes. Here we present a ca...

journal_title：Applied and environmental microbiology

authors： Xiao Z,Bergeron H,Grosse S,Beauchemin M,Garron ML,Shaya D,Sulea T,Cygler M,Lau PC

更新日期：2008-02-01 00:00:00

abstract：:Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean...

journal_title：Applied and environmental microbiology

authors： Demezas DH,Reardon TB,Watson JM,Gibson AH

更新日期：1991-12-01 00:00:00

abstract：:Carnobacterium maltaromaticum UAL307, isolated from fresh pork, exhibits potent activity against a number of gram-positive organisms, including numerous Listeria species. Three bacteriocins were isolated from culture supernatant, and using matrix-assisted laser desorption ionization-time of flight mass spectrometry an...

journal_title：Applied and environmental microbiology

authors： Martin-Visscher LA,van Belkum MJ,Garneau-Tsodikova S,Whittal RM,Zheng J,McMullen LM,Vederas JC

更新日期：2008-08-01 00:00:00

abstract：:Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybr...

journal_title：Applied and environmental microbiology

authors： Sajjaphan K,Shapir N,Wackett LP,Palmer M,Blackmon B,Tomkins J,Sadowsky MJ

更新日期：2004-07-01 00:00:00

abstract：:Traditional microscope-based estimates of species richness of aquatic hyphomycetes depend upon the ability of the species in the community to sporulate. Molecular techniques which detect DNA from all stages of the life cycle could potentially circumvent the problems associated with traditional methods. Leaf disks from...

journal_title：Applied and environmental microbiology

authors： Nikolcheva LG,Cockshutt AM,Bärlocher F

更新日期：2003-05-01 00:00:00

abstract：:We studied the growth of six culturable bacterial lineages from coastal North Sea picoplankton in environmental samples under different incubation conditions. The grazing pressure of heterotrophic nanoflagellates (HNF) was reduced either by double prefiltration through 0.8- micro m-pore-size filters or by 10-fold dilu...

journal_title：Applied and environmental microbiology

authors： Beardsley C,Pernthaler J,Wosniok W,Amann R

更新日期：2003-05-01 00:00:00

abstract：:Comparisons of animals bearing and lacking microorganisms can offer valuable insight into the interactions between animal hosts and their resident microbiota. Most hosts are naturally infected, and therefore, these comparisons require specific procedures (e.g., antibiotic treatment or physical exclusion of microorgani...

journal_title：Applied and environmental microbiology

authors： Ridley EV,Wong AC,Douglas AE

更新日期：2013-05-01 00:00:00

abstract：:The structure and composition of the oocyst wall are primary factors determining the survival and hydrologic transport of Cryptosporidium parvum oocysts outside the host. Microscopic and biochemical analyses of whole oocysts and purified oocyst walls were undertaken to better understand the inactivation kinetics and h...

journal_title：Applied and environmental microbiology

authors： Jenkins MB,Eaglesham BS,Anthony LC,Kachlany SC,Bowman DD,Ghiorse WC

更新日期：2010-03-01 00:00:00

abstract：:4-Ethoxy-3-methoxyphenylglycerol-gamma-formyl ester (compound IV) was identified as a degradation product of both 4-ethoxy-3-methoxyphenylglycerol-beta-syringaldehyde ether (compound I) and 4-ethoxy-3-methoxyphenylglycerol-beta-2,6-dimethoxyphenyl ether (compound II) by a ligninolytic culture of Coriolus versicolor. A...

journal_title：Applied and environmental microbiology

authors： Kawai S,Umezawa T,Higuchi T

更新日期：1985-12-01 00:00:00

abstract：:Restriction fragment length polymorphism (RFLP) and vegetative compatibility analyses were undertaken to assess genetic relationships among 52 isolates of Fusarium oxysporum f. sp. vasinfectum of worldwide origin and representing race A, 3, or 4 on cotton plants. Ten distinct vegetative compatibility groups (VCGs) wer...

journal_title：Applied and environmental microbiology

authors： Fernandez D,Assigbese K,Dubois MP,Geiger JP

更新日期：1994-11-01 00:00:00

abstract：:The Arthrobacter sp. strain SU 4-chlorobenzoate (4-CBA) dehalogenation pathway converts 4-CBA to 4-hydroxybenzoate (4-HBA). The pathway operon contains the genes fcbA, fcbB, and fcbC (A. Schmitz, K. H. Gartemann, J. Fiedler, E. Grund, and R. Eichenlaub, Appl. Environ. Microbiol. 58:4068-4071, 1992). Genes fcbA and fcb...

journal_title：Applied and environmental microbiology

authors： Zhuang Z,Gartemann KH,Eichenlaub R,Dunaway-Mariano D

更新日期：2003-05-01 00:00:00

abstract：:Short peptides of 11 residues were synthesized and tested against the economically important plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and Xanthomonas vesicatoria and compared to the previously described peptide Pep3 (WKLFKKILKVL-NH(2)). The antimicrobial activity of Pep3 and 22 analogues was ...

journal_title：Applied and environmental microbiology

authors： Ferre R,Badosa E,Feliu L,Planas M,Montesinos E,Bardají E

更新日期：2006-05-01 00:00:00

abstract：:A field evaluation of double violet agar for the isolation and presumptive identification of Klebsiella pneumoniae from water has been performed. Water from the North Oconee River, Clarke County, Ga., was cultured for presence of klebsiellae using the membrane filter technique. Colonies were presumptively identified a...

journal_title：Applied and environmental microbiology

authors： Campbell LM,Roth IL,Klein RD

更新日期：1976-02-01 00:00:00

abstract：:Fusarium oxysporum isolated from roots of and soil around Baccharis species from Brazil produced the trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and 3'-OH T-2 (TC-1), whereas Fusarium sporotrichioides from the same source produced T-2 toxin, HT-2 toxin, acetyl T-2, neosolaniol, TC-1, 3'-OH HT-2 (TC-3), i...

journal_title：Applied and environmental microbiology

authors： Mirocha CJ,Abbas HK,Kommedahl T,Jarvis BB

更新日期：1989-01-01 00:00:00

abstract：:Extraintestinal growth of fecal bacteria can impair accurate assessment of watershed health. Anaerobic fecal bacteria belonging to the order Bacteroidales are attractive candidates for fecal source tracking because they have host-specific distributions and do not grow well in the presence of high oxygen concentrations...

journal_title：Applied and environmental microbiology

authors： Walters SP,Field KG

更新日期：2006-07-01 00:00:00

abstract：:The effects of long-term heavy metal deposition on microbial community structure and the level of bacterial community tolerance were studied along two different gradients in Scandinavian coniferous forest soils. One was near the Harjavalta smelter in Finland, and one was at Ronnskar in Sweden. Phospholipid fatty acid ...

journal_title：Applied and environmental microbiology

authors： Pennanen T,Frostegard A,Fritze H,Baath E

更新日期：1996-02-01 00:00:00

abstract：:The filter exclusion method was used to isolate Frankia strains from Myrica pennsylvanica (bayberry) root nodules collected at diverse sites in New Jersey. A total of 16 isolates from five locations were cultured. The isolates were characterized by morphological, chemical, physiological, and plant infectivity criteria...

journal_title：Applied and environmental microbiology

authors： Bloom RA,Lechevalier MP,Tate RL 3rd

更新日期：1989-09-01 00:00:00

abstract：:The circulation of human parechoviruses (HPeVs) in the population was studied by environmental surveillance comprising of molecular analyses of sewage samples (n = 89) that were collected from 15 different locations in The Netherlands. Samples were taken from sewage originating from schools (n = 9) or from parts of mu...

journal_title：Applied and environmental microbiology

authors： Lodder WJ,Wuite M,de Roda Husman AM,Rutjes SA

更新日期：2013-10-01 00:00:00

abstract：:Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (...

journal_title：Applied and environmental microbiology

authors： Olsson-Francis K,de la Torre R,Cockell CS

更新日期：2010-04-01 00:00:00

abstract：:A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed di...

journal_title：Applied and environmental microbiology

authors： Clough SJ,Flavier AB,Schell MA,Denny TP

更新日期：1997-03-01 00:00:00

abstract：:Prototrophic and growth factor-requiring strains of Alcaligenes spp. were used to study the effect of a protozoan, Tetrahymena pyriformis, on the degradation of p-aminobenzoate. The protozoan inhibited activity of the prototrophic bacterium by reducing its population size. For the growth factor-requiring strain of Alc...

journal_title：Applied and environmental microbiology

authors： Huang TC,Chang MC,Alexander M

更新日期：1981-01-01 00:00:00

abstract：:Currently only five (SEA-SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits.Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc) which is part of the Staphylococcus aureus genomic...

journal_title：Applied and environmental microbiology

authors： Schwendimann L,Merda D,Berger T,Denayer S,Feraudet Tarisse C,Kläui AJ,Messio S,Mistou MY,Nia Y,Hennekinne JA,Graber HU

更新日期：2020-12-18 00:00:00

abstract：:In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica en...

journal_title：Applied and environmental microbiology

authors： Winkelmann N,Jaekel U,Meyer C,Serrano W,Rachel R,Rosselló-Mora R,Harder J

更新日期：2010-02-01 00:00:00

abstract：:Estuarine systems are the major conduits for the transfer of nitrate from agricultural and other terrestrial-anthropogenic sources into marine ecosystems. Within estuarine sediments some microbially driven processes (denitrification and anammox) result in the net removal of nitrogen from the environment, while others ...

journal_title：Applied and environmental microbiology

authors： Smith CJ,Nedwell DB,Dong LF,Osborn AM

更新日期：2007-06-01 00:00:00

abstract：:There is currently little information on nonphosphorylated sugar epimerases, which are of potential interest for producing rare sugars. We found a gene (the TM0416 gene) encoding a putative d-tagatose-3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima We overexpressed the TM0416 gene ...

journal_title：Applied and environmental microbiology

authors： Shin SM,Cao TP,Choi JM,Kim SB,Lee SJ,Lee SH,Lee DW

更新日期：2017-05-01 00:00:00

abstract：:The possible origin of beef contamination and genetic diversity of Escherichia coli populations in beef cattle, on carcasses and ground beef, was examined by using random amplification of polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the fliC gene. E. coli was recovered...

journal_title：Applied and environmental microbiology

authors： Aslam M,Nattress F,Greer G,Yost C,Gill C,McMullen L

更新日期：2003-05-01 00:00:00

abstract：:Mean pH values in pooled samples of foregut, midgut, and hindgut from adult Melanoplus sanguinipes, which had been raised in the laboratory on barley shoots and wheat bran, were 5.15, 6.39, and 5.98, respectively. Homogenates of midgut/hindgut sections and frass (feces) yielded colony counts of bacteria by the spread ...

journal_title：Applied and environmental microbiology

authors： Mead LJ,Khachatourians GG,Jones GA

更新日期：1988-05-01 00:00:00

abstract：:Vibrio vulnificus naturally inhabits a variety of aquatic organisms, including oysters, and is the leading cause of seafood-related death in the United States. Strains of this bacterium are genetically classified into environmental (E) and clinical (C) genotypes, which correlate with source of isolation. E-genotype st...

journal_title：Applied and environmental microbiology

authors： Williams TC,Ayrapetyan M,Oliver JD

更新日期：2014-03-01 00:00:00

abstract：:Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and...

journal_title：Applied and environmental microbiology

authors： Müller D,Greune L,Heusipp G,Karch H,Fruth A,Tschäpe H,Schmidt MA

更新日期：2007-05-01 00:00:00

abstract：:In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a step particularly c...

journal_title：Applied and environmental microbiology

authors： Picard C,Ponsonnet C,Paget E,Nesme X,Simonet P

更新日期：1992-09-01 00:00:00