Engineering a Saccharomyces cerevisiae wine yeast that exhibits reduced ethanol production during fermentation under controlled microoxygenation conditions.

abstract：:The cyanobacterium Microcystis can produce microcystins, a family of toxins that are of major concern in water management. In several lakes, the average microcystin content per cell gradually declines from high levels at the onset of Microcystis blooms to low levels at the height of the bloom. Such seasonal dynamics m...

journal_title：Applied and environmental microbiology

authors： Kardinaal WE,Tonk L,Janse I,Hol S,Slot P,Huisman J,Visser PM

更新日期：2007-05-01 00:00:00

abstract：:Laboratory-scale soil microcosms containing different soils were permeated with CH(inf4) for up to 6 months to investigate their capacity to develop a methanotrophic community. Methane emissions were monitored continuously until steady states were established. The porous, coarse sand soil developed the greatest methan...

journal_title：Applied and environmental microbiology

authors： Kightley D,Nedwell DB,Cooper M

更新日期：1995-02-01 00:00:00

abstract：:The effects of different organic substrates on the abilities of anaerobic sediment enrichments to reductively dechlorinate polychlorinated biphenyls (PCBs) were studied. Sediments collected from a site previously contaminated with PCBs were dosed with additional PCBs (Aroclor 1242; approximately 300 ppm [300 mug/g], s...

journal_title：Applied and environmental microbiology

authors： Nies L,Vogel TM

更新日期：1990-09-01 00:00:00

abstract：:Many people in Japan often detect an unpleasant odor generated from laundry that is hung to dry indoors or when using their already-dried laundry. Such an odor is often described as a "wet-and-dirty-dustcloth-like malodor" or an "acidic or sweaty odor." In this study, we isolated the major microorganisms associated wi...

journal_title：Applied and environmental microbiology

authors： Kubota H,Mitani A,Niwano Y,Takeuchi K,Tanaka A,Yamaguchi N,Kawamura Y,Hitomi J

更新日期：2012-05-01 00:00:00

abstract：:A mixed methanogenic culture fed with glucose was perturbed with butyrate and formate to investigate the role of formate in the acetogenesis of butyrate. A free energy analysis suggests that formate rather than H(2) was the interspecies electron carrier for butyrate conversion into acetate for the culture studied. ...

journal_title：Applied and environmental microbiology

authors： Bae J,McCarty PL

更新日期：1993-02-01 00:00:00

abstract：:A soil bacterium capable of utilizing fluoranthene as the sole source of carbon and energy for growth was purified from a seven-member bacterial community previously isolated from a creosote waste site for its ability to degrade polycyclic aromatic hydrocarbons. By standard bacteriological methods, this bacterium was ...

journal_title：Applied and environmental microbiology

authors： Mueller JG,Chapman PJ,Blattmann BO,Pritchard PH

更新日期：1990-04-01 00:00:00

abstract：:The noncanonical alcohol dehydrogenase AlkJ is encoded on the alkane-metabolizing alk operon of the mesophilic bacterium Pseudomonas putida GPo1. To gain insight into the enzymology of AlkJ, we have produced the recombinant protein in Escherichia coli and purified it to homogeneity using His6 tag affinity and size exc...

journal_title：Applied and environmental microbiology

authors： Kirmair L,Skerra A

更新日期：2014-04-01 00:00:00

abstract：:Studies of the interaction of Legionella pneumophila with free-living amoebae showed that Naegleria lovaniensis and Acanthamoeba royreba could use L. pneumophila as a sole food source. However, growth of the amoebae on nonnutrient agar plates seeded with L. pneumophila was slower than growth on nonnutrient agar plates...

journal_title：Applied and environmental microbiology

authors： Tyndall RL,Domingue EL

更新日期：1982-10-01 00:00:00

abstract：:A variety of nitroaromatic compounds, including 2,4,6-trinitrotoluene (TNT), were reduced by hydrogen in the presence of enzyme preparations from Veillonella alkalescens. Consistent with the proposed reduction pathway, R-NO2 H2 leads to R-NO H2 leads to R-NHOH H2 leads to R-NH2, 3 mol of H2 was utilized per mol of nit...

journal_title：Applied and environmental microbiology

authors： McCormick NG,Feeherry FE,Levinson HS

更新日期：1976-06-01 00:00:00

abstract：:Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

journal_title：Applied and environmental microbiology

authors： Bian J,Fenno JC,Li C

更新日期：2012-03-01 00:00:00

abstract：:Both the periplasmic and the extracellular cellodextrinases from Bacteroides succinogenes S85 grown on Avicel microcrystalline cellulose were purified to homogeneity by column chromatography and characterized. Over 70% of the total cellobiosidase activity displayed by cells was accounted for by these enzymes. The peri...

journal_title：Applied and environmental microbiology

authors： Huang L,Forsberg CW

更新日期：1988-06-01 00:00:00

abstract：:Until recently, Amblyomma maculatum (the Gulf Coast tick) had garnered little attention compared to other species of human-biting ticks in the United States. A. maculatum is now recognized as the principal vector of Rickettsia parkeri, a pathogenic spotted fever group rickettsia (SFGR) that causes an eschar-associated...

journal_title：Applied and environmental microbiology

authors： Paddock CD,Fournier PE,Sumner JW,Goddard J,Elshenawy Y,Metcalfe MG,Loftis AD,Varela-Stokes A

更新日期：2010-05-01 00:00:00

abstract：:Algal biofuels represent one of the most promising means of sustainably replacing liquid fuels. However, significant challenges remain before alga-based fuels become competitive with fossil fuels. One of the largest challenges is the ability to harvest the algae in an economical and low-energy manner. In this article,...

journal_title：Applied and environmental microbiology

authors： Powell RJ,Hill RT

更新日期：2013-10-01 00:00:00

abstract：:Spore-forming Bacillus strains that produce extracellular poly-γ-glutamic acid were screened for their application to natto (fermented soybean food) fermentation. Among the 424 strains, including Bacillus subtilis and B. amyloliquefaciens, which we isolated from rice straw, 59 were capable of fermenting natto. Biotin ...

journal_title：Applied and environmental microbiology

authors： Kubo Y,Rooney AP,Tsukakoshi Y,Nakagawa R,Hasegawa H,Kimura K

更新日期：2011-09-01 00:00:00

abstract：:Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid...

journal_title：Applied and environmental microbiology

authors： Morlino GB,Tizzani L,Fleer R,Frontali L,Bianchi MM

更新日期：1999-11-01 00:00:00

abstract：:Great differences in capability to detect bacteriophages from urban sewage of the area of Barcelona existed among 115 strains of Bacteroides fragilis. The capability of six of the strains to detect phages in a variety of feces and wastewater was studied. Strains HSP40 and RYC4023 detected similar numbers of phages in ...

journal_title：Applied and environmental microbiology

authors： Puig A,Queralt N,Jofre J,Araujo R

更新日期：1999-04-01 00:00:00

abstract：:The diversity of microcystin-producing cyanobacteria in the western basin of Lake Erie was studied using sequence analysis of mcyA gene fragments. Distinct populations of potentially toxic Microcystis and Planktothrix were found in spatially isolated locations. This study highlights previously undocumented diversity o...

journal_title：Applied and environmental microbiology

authors： Rinta-Kanto JM,Wilhelm SW

更新日期：2006-07-01 00:00:00

abstract：:Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the lactase LacA (LacZ) from the thermophilic bacterium Thermoanaerobacter ethanolicus and the cellulase CelD, a cellulose-binding module (CBM) from Anaerocellum the...

journal_title：Applied and environmental microbiology

authors： Velikodvorskaya GA,Tikhonova TV,Gurvits ID,Karyagina AS,Lavrova NV,Sergienko OV,Tashlitskii VN,Lunina NA,Lunin VG

更新日期：2010-12-01 00:00:00

abstract：:Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to an...

journal_title：Applied and environmental microbiology

authors： Rodríguez E,Banchio C,Diacovich L,Bibb MJ,Gramajo H

更新日期：2001-09-01 00:00:00

abstract：:The inhibitory effects of sterigmatocystin, O-methylsterigmatocystin, and 5,6-dimethoxysterigmatocystin on the ATP synthesis system in mitochondria were compared with that of aflatoxin B1, which disturbs the respiratory chain in mitochondria. Sterigmatocystin and 5,6-dimethoxysterigmatocystin were found to uncouple th...

journal_title：Applied and environmental microbiology

authors： Kawai K,Nakamaru T,Nozawa Y,Maebayashi Y,Yamazaki M,Natori S

更新日期：1984-11-01 00:00:00

abstract：:Methanotrophs have multiple methane monooxygenases that are well known to be regulated by copper, i.e., a "copper switch." At low copper/biomass ratios the soluble methane monooxygenase (sMMO) is expressed while expression and activity of the particulate methane monooxygenase (pMMO) increases with increasing availabil...

journal_title：Applied and environmental microbiology

authors： Farhan Ul Haque M,Kalidass B,Bandow N,Turpin EA,DiSpirito AA,Semrau JD

更新日期：2015-11-01 00:00:00

abstract：:In this study, host-associated molecular markers and bacterial 16S rRNA gene community analysis using high-throughput sequencing were used to identify the sources of fecal pollution in environmental waters in Brisbane, Australia. A total of 92 fecal and composite wastewater samples were collected from different host g...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Staley C,Sadowsky MJ,Gyawali P,Sidhu JP,Palmer A,Beale DJ,Toze S

更新日期：2015-10-01 00:00:00

abstract：:Resistance to hops is a prerequisite for the capability of lactic acid bacteria to grow in beer and thus cause beer spoilage. Bactericidal hop compounds, mainly iso-alpha-acids, are described as ionophores which exchange H+ for cellular divalent cations, e.g., Mn2+, and thus dissipate ion gradients across the cytoplas...

journal_title：Applied and environmental microbiology

authors： Behr J,Israel L,Gänzle MG,Vogel RF

更新日期：2007-05-01 00:00:00

abstract：:Accurate enumeration of viruses within environmental samples is critical for investigations of the ecological role of viruses and viral infection within microbial communities. This report evaluates differences in viral and bacterial direct counts between estuarine sediment samples which were either immediately process...

journal_title：Applied and environmental microbiology

authors： Helton RR,Liu L,Wommack KE

更新日期：2006-07-01 00:00:00

abstract：:Volume 61, no. 12, p. 4505, Introduction, column 1, line 13, and column 2, line 5: reference 19, which refers to a toxicity test based on the inhibition of (beta)-glucuronidase, not (beta)-galactosidase, in E. coli, should not be cited. [This corrects the article on p. 4505 in vol. 61.]. ...

journal_title：Applied and environmental microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：1996-02-01 00:00:00

abstract：:A strain that efficiently degraded methyl tert-butyl ether (MTBE) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (TBA). This strain, a gram-positive methylotrophic bacterium identified as Mycobacterium austroafricanum IFP 2012, was also able to degrade tert-amyl methyl ether and tert...

journal_title：Applied and environmental microbiology

authors： François A,Mathis H,Godefroy D,Piveteau P,Fayolle F,Monot F

更新日期：2002-06-01 00:00:00

abstract：:We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of th...

journal_title：Applied and environmental microbiology

authors： Qian C,Wong CC,Swarup S,Chiam KH

更新日期：2013-08-01 00:00:00

abstract：:The lytic action of glycerol and sucrose esters of fatty acids with different carbon chain lengths on the exponentially growing cells of Bacillus subtilis 168 was investigated. Of each series of esters, glycerol dodecanoate and sucrose hexadecanoate were the most active. Lysis at 1 h after the addition of 0.1 mM glyce...

journal_title：Applied and environmental microbiology

authors： Tsuchido T,Ahn YH,Takano M

更新日期：1987-03-01 00:00:00

abstract：:One of the first steps in characterizing an ecosystem is to describe the organisms inhabiting it. For microbial studies, experimental limitations have hindered the ability to depict diverse communities. Here we describe oligonucleotide fingerprinting of rRNA genes (OFRG), a method that permits identification of arraye...

journal_title：Applied and environmental microbiology

authors： Valinsky L,Della Vedova G,Scupham AJ,Alvey S,Figueroa A,Yin B,Hartin RJ,Chrobak M,Crowley DE,Jiang T,Borneman J

更新日期：2002-07-01 00:00:00

abstract：:To obtain molecular insights into the action mode of antimicrobial activity of pediocin PA-1, the interactions between this bacteriocin and dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylglycerol (DMPG) model membranes have been investigated in D(2)O at pD 6 by Fourier transform infrared spectroscopy....

journal_title：Applied and environmental microbiology

authors： Gaussier H,Lefèvre T,Subirade M

更新日期：2003-11-01 00:00:00