Bacterial community in copper sulfide ores inoculated and leached with solution from a commercial-scale copper leaching plant.

Abstract:

:Most copper bioleaching plants operate with a high concentration of sulfate salts caused by the continuous addition of sulfuric acid and the recycling of the leaching solution. Since the bacteria involved in bioleaching have been generally isolated at low sulfate concentrations, the bacterial population in ores leached with the high-sulfate solution (1.25 M) employed in a copper production plant was investigated. The complexity of the original population was assessed by the length pattern of the spacer regions between the 16S and 23S rRNA genes, observed after PCR amplification of the DNA extracted from the leached ore. Six main spacers were distinguished by electrophoretic migration, but they could be further resolved into eight spacers by nucleotide sequence homology. The degree of homology was inferred from the electrophoretic migration of the heteroduplexes formed after hybridization. One of the spacers was indistinguishable from that found in Thiobacillus thiooxidans, four could be related to Thiobacillus ferrooxidans, and three could be related to Leptospirillum ferrooxidans. Only five of the spacers in the original sample could be recovered after culturing in media containing different inorganic energy source. Altogether, the results indicate that the bacteria in the leached ore formed a community composed of at least three species: a fairly homogeneous population of T. thiooxidans strains and two heterogeneous populations of T. ferrooxidans and L. ferrooxidans strains.

journal_name

Appl Environ Microbiol

authors

Espejo RT,Romero J

doi

10.1128/AEM.63.4.1344-1348.1997

keywords:

subject

Has Abstract

pub_date

1997-04-01 00:00:00

pages

1344-8

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

63

pub_type

杂志文章
  • Genetic characterization of plasmid-associated benzalkonium chloride resistance determinants in a Listeria monocytogenes strain from the 1998-1999 outbreak.

    abstract::Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02056-10

    authors: Elhanafi D,Dutta V,Kathariou S

    更新日期:2010-12-01 00:00:00

  • Physiological characterization of strain DCB-1, a unique dehalogenating sulfidogenic bacterium.

    abstract::Strain DCB-1 is an obligately anaerobic bacterium which carries out the reductive dehalogenation of halobenzoates and was previously known to grow only on pyruvate plus 20% ruminal fluid. When various electron acceptors were supplied, thiosulfate and sulfite were found to stimulate growth. Sulfide was produced from th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.12.2938-2943.1988

    authors: Stevens TO,Linkfield TG,Tiedje JM

    更新日期:1988-12-01 00:00:00

  • Formation of Crystalline delta-Endotoxin or Poly-beta-Hydroxybutyric Acid Granules by Asporogenous Mutants of Bacillus thuringiensis.

    abstract::Parental strains and asporogenous mutants of Bacillus thuringiensis subspp. kurstaki and aizawai produced high yields of delta-endotoxin on M medium, which contained 330 mug of potassium per ml, but not on ST and ST-a media, each of which contained only 11 mug of potassium per ml. On ST and ST-a media, refractile gran...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.6.1473-1480.1982

    authors: Wakisaka Y,Masaki E,Nishimoto Y

    更新日期:1982-06-01 00:00:00

  • Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification.

    abstract::DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.4.1378-1382.1996

    authors: Coleman SS,Melanson DM,Biosca EG,Oliver JD

    更新日期:1996-04-01 00:00:00

  • Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger beta-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes.

    abstract::The beta-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 2,412-bp open reading frame that encodes a 804-amino-acid propeptide. The 778-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.12.5512-5519.2001

    authors: La Grange DC,Pretorius IS,Claeyssens M,van Zyl WH

    更新日期:2001-12-01 00:00:00

  • Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a beta-mannanase from the extremely thermophilic bacterium "Caldocellum saccharolyticum".

    abstract::A lambda recombinant phage expressing beta-mannanase activity in Escherichia coli has been isolated from a genomic library of the extremely thermophilic anaerobe "Caldocellum saccharolyticum." The gene was cloned into pBR322 on a 5-kb BamHI fragment, and its location was obtained by deletion analysis. The sequence of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.3.694-700.1991

    authors: Lüthi E,Jasmat NB,Grayling RA,Love DR,Bergquist PL

    更新日期:1991-03-01 00:00:00

  • Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization.

    abstract::The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacte...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.7.2691-2696.1998

    authors: Llobet-Brossa E,Rosselló-Mora R,Amann R

    更新日期:1998-07-01 00:00:00

  • Killing of Staphylococcus aureus via Magnetic Hyperthermia Mediated by Magnetotactic Bacteria.

    abstract::Staphylococcus aureus is a common hospital and household pathogen. Given the emergence of antibiotic-resistant derivatives of this pathogen resulting from the use of antibiotics as general treatment, development of alternative therapeutic strategies is urgently needed. Here, we assess the feasibility of killing S. aur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04103-15

    authors: Chen C,Chen L,Yi Y,Chen C,Wu LF,Song T

    更新日期:2016-02-12 00:00:00

  • Extremely acidophilic protists from acid mine drainage host Rickettsiales-lineage endosymbionts that have intervening sequences in their 16S rRNA genes.

    abstract::During a molecular phylogenetic survey of extremely acidic (pH < 1), metal-rich acid mine drainage habitats in the Richmond Mine at Iron Mountain, Calif., we detected 16S rRNA gene sequences of a novel bacterial group belonging to the order Rickettsiales in the Alphaproteobacteria. The closest known relatives of this ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.9.5512-5518.2003

    authors: Baker BJ,Hugenholtz P,Dawson SC,Banfield JF

    更新日期:2003-09-01 00:00:00

  • Cell Wall Glycans Mediate Recognition of the Dairy Bacterium Streptococcus thermophilus by Bacteriophages.

    abstract::Receptors on the cell surfaces of bacterial hosts are essential during the infection cycle of bacteriophages. To date, the phage receptors of the industrial relevant dairy starter bacterium Streptococcus thermophilus remain elusive. Thus, we set out to identify cell surface structures that are involved in host recogni...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01847-18

    authors: Szymczak P,Filipe SR,Covas G,Vogensen FK,Neves AR,Janzen T

    更新日期:2018-11-15 00:00:00

  • Arthrobacter aurescens TC1 atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli.

    abstract::Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.7.4402-4407.2004

    authors: Sajjaphan K,Shapir N,Wackett LP,Palmer M,Blackmon B,Tomkins J,Sadowsky MJ

    更新日期:2004-07-01 00:00:00

  • Enterotoxin production and thermal resistance of Yersinia enterocolitica in milk.

    abstract::Three of 36 raw milk isolates of Yersinia enterocolitica produced enterotoxin in milk at 25 degrees C, but not at 4 degrees C. No strain tested could survive pasteurization. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.1.174-176.1980

    authors: Francis DW,Spaulding PL,Lovett J

    更新日期:1980-07-01 00:00:00

  • Toolbox Approaches Using Molecular Markers and 16S rRNA Gene Amplicon Data Sets for Identification of Fecal Pollution in Surface Water.

    abstract::In this study, host-associated molecular markers and bacterial 16S rRNA gene community analysis using high-throughput sequencing were used to identify the sources of fecal pollution in environmental waters in Brisbane, Australia. A total of 92 fecal and composite wastewater samples were collected from different host g...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02032-15

    authors: Ahmed W,Staley C,Sadowsky MJ,Gyawali P,Sidhu JP,Palmer A,Beale DJ,Toze S

    更新日期:2015-10-01 00:00:00

  • Purification and Properties of beta-1, 4-Xylanase from Aeromonas caviae W-61.

    abstract::Aeromonas caviae W-61, which was isolated from water samples at the Faculty of Agriculture, Tohoku University, produced beta-1, 4-xylanase (1,4-beta-d-xylan xylanohydrolase; EC 3.2.1.8) extracellularly. The xylanase was purified to homogeneity by using DEAE-Sephadex A-50, CM-Sephadex C-50, and Sephadex G-100 column ch...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.2.445-449.1991

    authors: Viet DN,Kamio Y,Abe N,Kaneko J,Izaki K

    更新日期:1991-02-01 00:00:00

  • Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits.

    abstract::During cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. The diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms wer...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00866-07

    authors: Hantsis-Zacharov E,Halpern M

    更新日期:2007-11-01 00:00:00

  • An improved PCR-restriction fragment length polymorphism (RFLP) method for the identification of cry1-type genes.

    abstract::The cry1-type genes of Bacillus thuringiensis represent the largest cry gene family, which contains 50 distinct holotypes. It is becoming more and more difficult to identify cry1-type genes using current methods because of the increasing number of cry1-type genes. In the present study, an improved PCR-restriction frag...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01983-13

    authors: Shu C,Liu D,Zhou Z,Cai J,Peng Q,Gao J,Song F,Zhang J

    更新日期:2013-11-01 00:00:00

  • Light-dependent degradation of nitrophenols by the phototrophic bacterium Rhodobacter capsulatus E1F1.

    abstract::Rhodobacter capsulatus E1F1, a phototrophic purple nonsulfur bacterium capable of photoassimilating nitrate or nitrite, grew phototrophically in the presence of mono- and dinitrophenols with acetate as a carbon source, the highest growth levels being obtained under microaerobic conditions. Utilization of 2,4-dinitroph...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.2.690-695.1992

    authors: Blasco R,Castillo F

    更新日期:1992-02-01 00:00:00

  • Permanent colonization by Lactobacillus casei is hindered by the low rate of cell division in mouse gut.

    abstract::Long residence times of probiotics in the intestinal tract would prolong their potential beneficial health effects and assist colonization. This study investigated the colonization potential of Lactobacillus casei Shirota in mouse intestine by using 5 (and 6)-carboxyfluorescein diacetate, succinimidyl ester (cFDA-SE) ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.2.670-674.2004

    authors: Lee YK,Ho PS,Low CS,Arvilommi H,Salminen S

    更新日期:2004-02-01 00:00:00

  • Transformation of mercuric chloride and methylmercury by the rumen microflora.

    abstract::The microflora in strained rumen fluid did not methylate or volatilize 203Hg2+ at detectable rates. However, there was an exponential decay in the concentration of added CH3Hg+, which was attributed to demethylation. The major product of demethylation was metallic mercury (Hg0), and it was released as a volatile produ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.4.626-636.1979

    authors: Kozak S,Forsberg CW

    更新日期:1979-10-01 00:00:00

  • Bradyrhizobia from wild Phaseolus, Desmodium, and Macroptilium species in northern Mexico.

    abstract::rRNA genetic markers were analyzed in 97 isolates of nodule bacteria from six legume species in Chihuahua, Mexico. The most common genotypes were widely shared across host species and had 16S rRNA sequences identical to those of strains from an eastern North American legume (Amphicarpaea) that are closely related to B...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.4.2044-2048.2002

    authors: Parker MA

    更新日期:2002-04-01 00:00:00

  • Structure-activity relationships in the peptide antibiotic nisin: role of dehydroalanine 5.

    abstract::A mutant of the peptide antibiotic nisin in which the dehydroalanine residue at position 5 has been replaced by an alanine has been produced and structurally characterized. It is shown to have activity very similar to that of wild-type nisin in inhibiting growth of Lactococcus lactis and Micrococcus luteus but is very...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.8.2966-2969.1996

    authors: Chan WC,Dodd HM,Horn N,Maclean K,Lian LY,Bycroft BW,Gasson MJ,Roberts GC

    更新日期:1996-08-01 00:00:00

  • Marine cyanophages demonstrate biogeographic patterns throughout the global ocean.

    abstract::Myoviruses and podoviruses that infect cyanobacteria are the two major groups of marine cyanophages, but little is known of how their phylogenetic lineages are distributed in different habitats. In this study, we analyzed the phylogenetic relationships of cyanopodoviruses and cyanomyoviruses based on the existing geno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02483-14

    authors: Huang S,Zhang S,Jiao N,Chen F

    更新日期:2015-01-01 00:00:00

  • A Nonautochthonous U.S. Strain of Vibrio parahaemolyticus Isolated from Chesapeake Bay Oysters Caused the Outbreak in Maryland in 2010.

    abstract:UNLABELLED:In the summer of 2010, Vibrio parahaemolyticus caused an outbreak in Maryland linked to the consumption of oysters. Strains isolated from both stool and oyster samples were indistinguishable by pulsed-field gel electrophoresis (PFGE). However, the oysters contained other potentially pathogenic V. parahaemoly...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00096-16

    authors: Haendiges J,Jones J,Myers RA,Mitchell CS,Butler E,Toro M,Gonzalez-Escalona N

    更新日期:2016-05-16 00:00:00

  • Ruminal Degradation of Rumen-Protected Glucose Influences the Ruminal Microbiota and Metabolites in Early-Lactation Dairy Cows.

    abstract::Rumen-protected glucose (RPG) plays an important role in alleviating the negative energy balance of dairy cows. This study used a combination of rumen microbes 16S and metabolomics to elucidate the changes of rumen microbial composition and rumen metabolites of different doses of RPG's rumen degradation part in early-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01908-20

    authors: Wang Y,Nan X,Zhao Y,Wang Y,Jiang L,Xiong B

    更新日期:2021-01-04 00:00:00

  • Clustering of marine bacteria in seawater enrichments.

    abstract::Seawater enrichments of marine bacteria clustered in 20- to 50-(mu)m-wide bands near air-water interfaces. The cells within the band travelled at up to 212 (mu)m s(sup-1) and at an average speed of 163 (mu)m s(sup-1). Mean cell speeds peaked mid-run at 187 (mu)m s(sup-1). At the end of the run, bacteria reversed direc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.10.3716-3721.1996

    authors: Mitchell JG,Pearson L,Dillon S

    更新日期:1996-10-01 00:00:00

  • Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR.

    abstract::Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02855-06

    authors: Müller D,Greune L,Heusipp G,Karch H,Fruth A,Tschäpe H,Schmidt MA

    更新日期:2007-05-01 00:00:00

  • Investigation of malic acid production in Aspergillus oryzae under nitrogen starvation conditions.

    abstract::Malic acid has great potential for replacing petrochemical building blocks in the future. For this application, high yields, rates, and titers are essential in order to sustain a viable biotechnological production process. Natural high-capacity malic acid producers like the malic acid producer Aspergillus flavus have ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01445-13

    authors: Knuf C,Nookaew I,Brown SH,McCulloch M,Berry A,Nielsen J

    更新日期:2013-10-01 00:00:00

  • Soil microbial responses to increased moisture and organic resources along a salinity gradient in a polar desert.

    abstract::Microbial communities in extreme environments often have low diversity and specialized physiologies suggesting a limited resistance to change. The McMurdo Dry Valleys (MDV) are a microbially dominated, extreme ecosystem currently undergoing climate change-induced disturbances, including the melting of massive buried i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03414-13

    authors: Van Horn DJ,Okie JG,Buelow HN,Gooseff MN,Barrett JE,Takacs-Vesbach CD

    更新日期:2014-05-01 00:00:00

  • Assessment of Giardia and Cryptosporidium spp. as a microbial source tracking tool for surface water: application in a mixed-use watershed.

    abstract::Knowledge of host specificity, combined with genomic sequencing of Giardia and Cryptosporidium spp., has demonstrated a microbial source tracking (MST) utility for these common waterborne microbes. To explore the source attribution potential of these pathogens, water samples were collected in a mixed rural-urban water...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02037-13

    authors: Prystajecky N,Huck PM,Schreier H,Isaac-Renton JL

    更新日期:2014-04-01 00:00:00

  • Simultaneous Nitrification and Denitrification in Aerobic Chemostat Cultures of Thiosphaera pantotropha.

    abstract::Thiosphaera pantotropha is capable of simultaneous heterotrophic nitrification and aerobic denitrification. Consequently, its nitrification potential could not be judged from nitrite accumulation, but was estimated from complete nitrogen balances. The maximum rate of nitrification obtained during these experiments was...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.11.2812-2818.1988

    authors: Robertson LA,van Niel EW,Torremans RA,Kuenen JG

    更新日期:1988-11-01 00:00:00