Interference of peptone and tyrosine with the lignin peroxidase assay.

abstract：:The influence of glucose concentration on Cd, Cu, Hg, and Zn toxicity to a Klebsiella sp. was studied by following the degradation of C-labeled glucose at pH 6.0. Uptake of C into the cells was also determined. The carbon concentrations ranged from 0.01 to 40 mg liter, which are equivalent to soluble C concentrations ...

journal_title：Applied and environmental microbiology

authors： Brynhildsen L,Lundgren BV,Allard B,Rosswall T

更新日期：1988-07-01 00:00:00

abstract：:The nutristat, a substrate concentration-controlled continuous culture, was used to grow pentachlorophenol (PCP)-degrading microorganisms. The PCP concentration control system consisted of on-line measurement of the PCP concentration in the culture vessel with a tangential filter and a flowthrough spectrophotometer. W...

journal_title：Applied and environmental microbiology

authors： Rutgers M,Bogte JJ,Breure AM,van Andel JG

更新日期：1993-10-01 00:00:00

abstract：:A metagenomic library of a petroleum-contaminated soil was constructed in a fosmid vector that allowed heterologous expression of metagenomic DNA. The library, consisting of 6.5 Gb of metagenomic DNA, was screened for extradiol dioxygenase (Edo) activity using catechol and 2,3-dihydroxybiphenyl as the substrates. Fift...

journal_title：Applied and environmental microbiology

authors： Terrón-González L,Martín-Cabello G,Ferrer M,Santero E

更新日期：2016-04-04 00:00:00

abstract：:Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...

journal_title：Applied and environmental microbiology

authors： Uchiyama H,Nakajima T,Yagi O,Nakahara T

更新日期：1992-09-01 00:00:00

abstract：:Norovirus is one of the most common causes of acute viral gastroenteritis. The virus is spread via the fecal-oral route, most commonly from infected food and water, but several outbreaks have originated from contamination of surfaces with infectious virus. In this study, a close surrogate of human norovirus causing ga...

journal_title：Applied and environmental microbiology

authors： Warnes SL,Summersgill EN,Keevil CW

更新日期：2015-02-01 00:00:00

abstract：:LeLoir glycosyltransferases are important biocatalysts for the production of glycosidic bonds in natural products, chiral building blocks, and pharmaceuticals. Trehalose transferase (TreT) is of particular interest since it catalyzes the stereo- and enantioselective α,α-(1→1) coupling of a nucleotide sugar donor and m...

journal_title：Applied and environmental microbiology

authors： Mestrom L,Marsden SR,Dieters M,Achterberg P,Stolk L,Bento I,Hanefeld U,Hagedoorn PL

更新日期：2019-04-04 00:00:00

abstract：:Methanol is already an important carbon feedstock in the chemical industry, but it has found only limited application in biotechnological production processes. This can be mostly attributed to the inability of most microbial platform organisms to utilize methanol as a carbon and energy source. With the aim to turn met...

journal_title：Applied and environmental microbiology

authors： Witthoff S,Schmitz K,Niedenführ S,Nöh K,Noack S,Bott M,Marienhagen J

更新日期：2015-03-01 00:00:00

abstract：:Despite the increasing knowledge of Synechococcus spp. and their co-occurring cyanophages in oceanic and coastal water, little is known about their abundance, distribution, and interactions in the Chesapeake Bay estuarine ecosystem. A 5-year interannual survey shows that Synechococcus spp. and their phages are persist...

journal_title：Applied and environmental microbiology

authors： Wang K,Wommack KE,Chen F

更新日期：2011-11-01 00:00:00

abstract：:beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...

journal_title：Applied and environmental microbiology

authors： Araujo A,Ward OP

更新日期：1990-06-01 00:00:00

abstract：:Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/...

journal_title：Applied and environmental microbiology

authors： Severino P,Dussurget O,Vêncio RZ,Dumas E,Garrido P,Padilla G,Piveteau P,Lemaître JP,Kunst F,Glaser P,Buchrieser C

更新日期：2007-10-01 00:00:00

abstract：:In a batch of barley associated with field cases of mycotoxic porcine nephropathy and containing ochratoxin A and citrinin, the mycoflora were isolated by parallel incubation at 10 and 25 degrees C. Subsequently, the isolated cultures were checked for production of nephrotoxins (xanthomegnin, viomellein, ochratoxin, a...

journal_title：Applied and environmental microbiology

authors： Hald B,Christensen DH,Krogh P

更新日期：1983-12-01 00:00:00

abstract：:The aerobic intestinal microflora of 2-week-old herring (Clupea harengus) larvae was characterized by using conventional microbiological methods and electron microscopy. Larvae were hatched and kept in filtered seawater or in seawater with penicillin and streptomycin. The gastrointestinal tract of herring larvae is es...

journal_title：Applied and environmental microbiology

authors： Hansen GH,Strøm E,Olafsen JA

更新日期：1992-02-01 00:00:00

abstract：:The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, a...

journal_title：Applied and environmental microbiology

authors： Kurz M,Burch AY,Seip B,Lindow SE,Gross H

更新日期：2010-08-01 00:00:00

abstract：:The subunit composition of the extracellular complex from Clostridium thermocellum was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Twenty-six bands, representing proteins with apparent molecular sizes ranging from 37,500 to 185,000 Da, could be detected by silver staining. Cultiva...

journal_title：Applied and environmental microbiology

authors： Kohring S,Wiegel J,Mayer F

更新日期：1990-12-01 00:00:00

abstract：:Bifidobacteria constitute a specific group of commensal bacteria that inhabit the gastrointestinal tracts of humans and other mammals. Bifidobacterium breve UCC2003 has previously been shown to utilize several plant-derived carbohydrates that include cellodextrins, starch, and galactan. In the present study, we invest...

journal_title：Applied and environmental microbiology

authors： Egan M,O'Connell Motherway M,Ventura M,van Sinderen D

更新日期：2014-07-01 00:00:00

abstract：:Three expedition huts in the Ross Sea region of Antarctica, built between 1901 and 1911 by Robert F. Scott and Ernest Shackleton, sheltered and stored the supplies for up to 48 men for 3 years during their explorations and scientific investigation in the South Pole region. The huts, built with wood taken to Antarctica...

journal_title：Applied and environmental microbiology

authors： Blanchette RA,Held BW,Jurgens JA,McNew DL,Harrington TC,Duncan SM,Farrell RL

更新日期：2004-03-01 00:00:00

abstract：:Trehalose, a disaccharide accumulated by many microorganisms, acts as a protectant during periods of physiological stress, such as salinity and desiccation. Previous studies reported that the trehalose biosynthetic genes (otsA, treS, and treY) in Bradyrhizobium japonicum were induced by salinity and desiccation stress...

journal_title：Applied and environmental microbiology

authors： Sugawara M,Cytryn EJ,Sadowsky MJ

更新日期：2010-02-01 00:00:00

abstract：:A novel method of detecting extracellular protease activity at biofilm-substratum interfaces was developed. This method utilizes fluorescent molecules bound to cellulose substrata with a lectin. Extracellular proteases degrade the lectin and release the fluorochrome into solution. This new technique and a standard dis...

journal_title：Applied and environmental microbiology

authors： Francoeur SN,Wetzel RG,Neely RK

更新日期：2001-09-01 00:00:00

abstract：:Glycogen debranching enzyme (GDE) has 4-alpha-glucanotransferase and amylo-1,6-glucosidase activities in the single polypeptide chain. We analyzed the detailed action profile of GDE from Saccharomyces cerevisiae on amylose and tested whether GDE catalyzes cyclization of amylose. GDE treatment resulted in a rapid reduc...

journal_title：Applied and environmental microbiology

authors： Yanase M,Takata H,Takaha T,Kuriki T,Smith SM,Okada S

更新日期：2002-09-01 00:00:00

abstract：:Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests th...

journal_title：Applied and environmental microbiology

authors： Zozaya-Hinchliffe M,Martin DH,Ferris MJ

更新日期：2008-03-01 00:00:00

abstract：:Mold-damaged building materials may contain biologically active agents, such as (1-->3)-beta-D-glucan, allergens, and mycotoxins, which have been associated with adverse health effects. The release of these components from contaminated surfaces into the air is not well understood. The purpose of this study was to char...

journal_title：Applied and environmental microbiology

authors： Seo SC,Reponen T,Levin L,Borchelt T,Grinshpun SA

更新日期：2008-02-01 00:00:00

abstract：:Nitrate was formed from ammonium at pH 3.2 to 6.1 in suspensions of a naturally acid forest soil; the maximum rates of formation occurred at ca. pH 4 to 5. Nitrate was also formed from soil nitrogen in suspensions incubated at 50 degrees C. Autotrophic nitrifying bacteria could not be isolated from this soil. Enrichme...

journal_title：Applied and environmental microbiology

authors： Stroo HF,Klein TM,Alexander M

更新日期：1986-11-01 00:00:00

abstract：:Searles Lake occupies a closed basin harboring salt-saturated, alkaline brines that have exceptionally high concentrations of arsenic oxyanions. Strain SLAS-1(T) was previously isolated from Searles Lake (R. S. Oremland, T. R. Kulp, J. Switzer Blum, S. E. Hoeft, S. Baesman, L. G. Miller, and J. F. Stolz, Science 308:1...

journal_title：Applied and environmental microbiology

authors： Blum JS,Han S,Lanoil B,Saltikov C,Witte B,Tabita FR,Langley S,Beveridge TJ,Jahnke L,Oremland RS

更新日期：2009-04-01 00:00:00

abstract：:Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...

journal_title：Applied and environmental microbiology

authors： Morick D,Faigenbaum O,Smirnov M,Fellig Y,Inbal A,Kotler M

更新日期：2015-05-15 00:00:00

abstract：:Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...

journal_title：Applied and environmental microbiology

authors： Desjardins AE,Plattner RD,Vanmiddlesworth F

更新日期：1986-03-01 00:00:00

abstract：:The type III secretion system (T3SS) is a specialized secretion apparatus that is commonly used by many plant and animal pathogenic bacteria to deliver proteins, termed effectors, to the interior of the host cells. These effectors suppress host defenses and interfere with signal transduction pathways to promote infect...

journal_title：Applied and environmental microbiology

authors： Jiménez-Guerrero I,Pérez-Montaño F,Medina C,Ollero FJ,López-Baena FJ

更新日期：2017-02-15 00:00:00

abstract：:The als gene for alpha-acetolactate synthase of Lactococcus lactis MG1363 was cloned on a multicopy plasmid under the control of the inducible L. lactis lacA promoter. More than a hundredfold overproduction of alpha-acetolactate synthase was obtained in L. lactis under inducing conditions as compared with that of the ...

journal_title：Applied and environmental microbiology

authors： Platteeuw C,Hugenholtz J,Starrenburg M,van Alen-Boerrigter I,de Vos WM

更新日期：1995-11-01 00:00:00

abstract：:The extremely thermoacidophilic archaeon Metallosphaera sedula (optimum growth temperature, 73°C, pH 2.0) grows chemolithoautotrophically on metal sulfides or molecular hydrogen by employing the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) carbon fixation cycle. This cycle adds two CO2 molecules to acetyl coenzyme ...

journal_title：Applied and environmental microbiology

authors： Hawkins AB,Adams MW,Kelly RM

更新日期：2014-04-01 00:00:00

abstract：:Enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC, respectively) strains are closely related human pathogens that are responsible for food-borne epidemics in many countries. Integration host factor (IHF) and the locus of enterocyte effacement-encoded regulator (Ler) are needed for the expression o...

journal_title：Applied and environmental microbiology

authors： Li M,Rosenshine I,Tung SL,Wang XH,Friedberg D,Hew CL,Leung KY

更新日期：2004-09-01 00:00:00

abstract：:Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage. Because of their efficiency and simplicity, serine integrases are also used for divers...

journal_title：Applied and environmental microbiology

authors： Fogg PCM,Haley JA,Stark WM,Smith MCM

更新日期：2017-02-15 00:00:00