Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection.

abstract：:The variable-region genes of monoclonal antibody against Bacillus cereus spores were cloned from mouse hybridoma cells by reverse transcription-PCR. The heavy- and light-chain variable-region genes were connected by a 45-base linker DNA to allow folding of the fusion protein into a functional tertiary structure. For d...

journal_title：Applied and environmental microbiology

authors： Koo K,Foegeding PM,Swaisgood HE

更新日期：1998-07-01 00:00:00

abstract：:The recent advent of tools enabling statistical inferences to be drawn from comparisons of microbial communities has enabled the focus of microbial ecology to move from characterizing biodiversity to describing the distribution of that biodiversity. Although statistical tools have been developed to compare community s...

journal_title：Applied and environmental microbiology

authors： Schloss PD,Handelsman J

更新日期：2006-10-01 00:00:00

abstract：:Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7 degrees C for 15 s in a local past...

journal_title：Applied and environmental microbiology

authors： Ayele WY,Svastova P,Roubal P,Bartos M,Pavlik I

更新日期：2005-03-01 00:00:00

abstract：:Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 microns in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two co...

journal_title：Applied and environmental microbiology

authors： Berk SG,Ting RS,Turner GW,Ashburn RJ

更新日期：1998-01-01 00:00:00

abstract：:The deep-sea hydrothermal vent shrimp Rimicaris exoculata largely depends on a dense epibiotic chemoautotrophic bacterial community within its enlarged cephalothoracic chamber. However, our understanding of shrimp-bacterium interactions is limited. In this report, we focused on the deltaproteobacterial epibiont of R. ...

journal_title：Applied and environmental microbiology

authors： Jiang L,Liu X,Dong C,Huang Z,Cambon-Bonavita MA,Alain K,Gu L,Wang S,Shao Z

更新日期：2020-04-01 00:00:00

abstract：:A genetic transformation system with similarities to those reported for gram-negative bacteria was found to be associated with membrane vesicles of the ruminal cellulolytic genus Ruminococcus. Double-stranded DNA was recovered from the subcellular particulate fraction of all the cellulolytic ruminococci examined. Elec...

journal_title：Applied and environmental microbiology

authors： Klieve AV,Yokoyama MT,Forster RJ,Ouwerkerk D,Bain PA,Mawhinney EL

更新日期：2005-08-01 00:00:00

abstract：:The impact of feed supplementation with bambermycin, monensin, narasin, virginiamycin, chlortetracycline, penicillin, salinomycin, and bacitracin on the distribution of Escherichia coli pathotypes in broiler chickens was investigated using an E. coli virulence DNA microarray. Among 256 E. coli isolates examined, 59 (2...

journal_title：Applied and environmental microbiology

authors： Bonnet C,Diarrassouba F,Brousseau R,Masson L,Topp E,Diarra MS

更新日期：2009-11-01 00:00:00

abstract：:Intracellular granules containing ferric and ferrous iron formed in Shewanella putrefaciens CN32 during dissimilatory reduction of solid-phase ferric iron. It is the first in situ detection at high resolution (150 nm) of a mixed-valence metal particle residing within a prokaryotic cell. The relationship of the interna...

journal_title：Applied and environmental microbiology

authors： Glasauer S,Langley S,Boyanov M,Lai B,Kemner K,Beveridge TJ

更新日期：2007-02-01 00:00:00

abstract：:We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuat...

journal_title：Applied and environmental microbiology

authors： Willyerd KL,Kemp AM,Dawe AL

更新日期：2009-08-01 00:00:00

abstract：:Electrophoretic karyotypes (EKs) of 83 isolates were variable within agricultural and natural populations of Sclerotinia sclerotiorum, as well as among S. sclerotiorum, Sclerotinia minor, and Sclerotinia trifoliorum. Variation in EKs was not observed within six mitotic or three meiotic lineages of isolates. EKs of 8 t...

journal_title：Applied and environmental microbiology

authors： Errampalli D,Kohn LM

更新日期：1996-11-01 00:00:00

abstract：:This study evaluated the influence of parameters relevant for cheese making on histamine formation by Streptococcus thermophilus. Strains possessing a histidine decarboxylase (hdcA) gene represented 6% of the dairy isolates screened. The most histaminogenic, S. thermophilus PRI60, exhibited in skim milk a high basal l...

journal_title：Applied and environmental microbiology

authors： Rossi F,Gardini F,Rizzotti L,La Gioia F,Tabanelli G,Torriani S

更新日期：2011-04-01 00:00:00

abstract：:Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC50) of the allelopathic fraction...

journal_title：Applied and environmental microbiology

authors： Li FM,Hu HY

更新日期：2005-11-01 00:00:00

abstract：:Acinetobacter baumannii is a metabolically versatile pathogen that causes severe infections in compromised patients. However, little is known about the genes and factors involved in its basic physiology and virulence properties. Insertion mutagenesis was used to initiate the identification and characterization of some...

journal_title：Applied and environmental microbiology

authors： Dorsey CW,Tomaras AP,Actis LA

更新日期：2002-12-01 00:00:00

abstract：:Differences in biological activities (hemagglutination, hemolytic, leukotoxic, and virulence) and ribotypes between the two subspecies of Fusobacterium necrophorum of bovine ruminal and liver abscess origins were investigated. Hemagglutination activity was present in all hepatic, but only some ruminal, strains of Fuso...

journal_title：Applied and environmental microbiology

authors： Okwumabua O,Tan Z,Staats J,Oberst RD,Chengappa MM,Nagaraja TG

更新日期：1996-02-01 00:00:00

abstract：:Pseudomonas fluorescens isolates 13525 and 2-79 were grown in Luria broth and low-nutrient medium (LNM). Pililike fibrils were very rarely produced in Luria broth but were abundantly produced in LNM. In LNM the pili were peritrichously distributed and had diameters ranging from 3 to 8 nm. Pili were purified from strai...

journal_title：Applied and environmental microbiology

authors： Vesper SJ

更新日期：1987-07-01 00:00:00

abstract：:The practical task of adapting an original potentiometric technique to the bacteriological analysis of water is discussed. Various laboratory strains of organisms belonging to the usual aquatic flora were inoculated one by one in a minimal lactose broth supplied with lipoic (thioctic) acid. The time evolution of the r...

journal_title：Applied and environmental microbiology

authors： Charriere G,Jouenne T,Lemeland JF,Selegny E,Junter GA

更新日期：1984-01-01 00:00:00

abstract：:Forty-nine typical and atypical enteropathogenic Escherichia coli (EPEC) strains belonging to different serotypes and isolated from humans, pets (cats and dogs), farm animals (bovines, sheep, and rabbits), and wild animals (monkeys) were investigated for virulence markers and clonal similarity by pulsed-field gel elec...

journal_title：Applied and environmental microbiology

authors： Moura RA,Sircili MP,Leomil L,Matté MH,Trabulsi LR,Elias WP,Irino K,Pestana de Castro AF

更新日期：2009-12-01 00:00:00

abstract：:Acid-adapted strains of Escherichia coli K-12 W3110 were obtained by serial culture in medium buffered at pH 4.6 (M. M. Harden, A. He, K. Creamer, M. W. Clark, I. Hamdallah, K. A. Martinez, R. L. Kresslein, S. P. Bush, and J. L. Slonczewski, Appl Environ Microbiol 81:1932-1941, 2015, https://doi.org/10.1128/AEM.03494-...

journal_title：Applied and environmental microbiology

authors： He A,Penix SR,Basting PJ,Griffith JM,Creamer KE,Camperchioli D,Clark MW,Gonzales AS,Chávez Erazo JS,George NS,Bhagwat AA,Slonczewski JL

更新日期：2017-05-31 00:00:00

abstract：:Vibrio vulnificus is a human pathogen associated with consumption of raw oysters. During the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured by ordinary bacteriological methods. For this reason, another means of detecting this bacterium is necessary. In the pre...

journal_title：Applied and environmental microbiology

authors： Brauns LA,Hudson MC,Oliver JD

更新日期：1991-09-01 00:00:00

abstract：:Water samples from rivers, streams, ponds, and activated sewage were tested for the presence of bacteria which utilize 2,4-dichlorophenoxyacetic acid (2,4-D) as a sole source of carbon. Seventy percent of the attempted enrichments yielded pure cultures of 2,4-D-metabolizing bacteria. All but 1 of the 30 isolates were ...

journal_title：Applied and environmental microbiology

authors： Amy PS,Schulke JW,Frazier LM,Seidler RJ

更新日期：1985-05-01 00:00:00

abstract：:Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and acidic heathland...

journal_title：Applied and environmental microbiology

authors： Herrmann M,Saunders AM,Schramm A

更新日期：2009-05-01 00:00:00

abstract：:The transport of branched-chain amino acids in Streptococcus thermophilus was energy dependent. The metabolic inhibitors of glycolysis and ATPase enzymes were active, but the proton-conducting uncouplers were not. Transport was optimal at temperatures of between 30 and 45 degrees C and at pH 7.0 for the three amino ac...

journal_title：Applied and environmental microbiology

authors： Akpemado KM,Bracquart PA

更新日期：1983-01-01 00:00:00

abstract：:Salmonella spp. are remarkably adaptable pathogens, and this adaptability allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amid the native microbiota remain poorly understood. Here, we aimed to uncover the mechanisms that enable ...

journal_title：Applied and environmental microbiology

authors： George AS,Cox CE,Desai P,Porwollik S,Chu W,de Moraes MH,McClelland M,Brandl MT,Teplitski M

更新日期：2018-02-14 00:00:00

abstract：:Acetaldehyde is a toxic compound produced by Saccharomyces cerevisiae cells under several growth conditions. The adverse effects of this molecule are important, as significant amounts accumulate inside the cells. By means of global gene expression analyses, we have detected the effects of acetaldehyde addition in the ...

journal_title：Applied and environmental microbiology

authors： Aranda A,del Olmo ML

更新日期：2004-04-01 00:00:00

abstract：:Twenty-six lactic acid bacterium strains isolated from European dairy products were identified as Streptococcus thermophilus and characterized by bacterial growth and exopolysaccharide (EPS)-producing capacity in milk and enriched milk medium. In addition, the acidification rates of the different strains were compared...

journal_title：Applied and environmental microbiology

authors： Vaningelgem F,Zamfir M,Mozzi F,Adriany T,Vancanneyt M,Swings J,De Vuyst L

更新日期：2004-02-01 00:00:00

abstract：:Intracellular pH (pH(in)) is a tightly regulated physiological parameter, which controls cell performance in all living systems. The purpose of this work was to evaluate if and how H(+) homeostasis is accomplished by an industrial wine strain of Saccharomyces cerevisiae while fermenting real must under the harsh winer...

journal_title：Applied and environmental microbiology

authors： Viana T,Loureiro-Dias MC,Loureiro V,Prista C

更新日期：2012-09-01 00:00:00

abstract：:A total of 1,794 migrating birds trapped at a coastal site in southern Sweden were sampled for detection of Campylobacter spp. All isolates phenotypically identified as Campylobacter jejuni and a subset of those identified as non-C. jejuni were identified to the species level by PCR-based techniques. C. jejuni was fou...

journal_title：Applied and environmental microbiology

authors： Waldenström J,Broman T,Carlsson I,Hasselquist D,Achterberg RP,Wagenaar JA,Olsen B

更新日期：2002-12-01 00:00:00

abstract：:The D-values of Bacillus pumilus spores on various devices ranged from 0.14 to 0.23 Mrads. The majority of devices displayed D-values equal to or less than the value obtained on filter paper. Increased resistivity was also encountered. ...

journal_title：Applied and environmental microbiology

authors： Prince HN

更新日期：1978-08-01 00:00:00

abstract：:Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), ...

journal_title：Applied and environmental microbiology

authors： Unnerstad H,Nilsson I,Ericsson H,Danielsson-Tham ML,Bille J,Bannerman E,Tham W

更新日期：1999-05-01 00:00:00

abstract：:The combination of ecological diversity with genetic and experimental tractability makes Drosophila a powerful model for the study of animal-associated microbial communities. Despite the known importance of yeasts in Drosophila physiology, behavior, and fitness, most recent work has focused on Drosophila-bacterial int...

journal_title：Applied and environmental microbiology

authors： Chandler JA,Eisen JA,Kopp A

更新日期：2012-10-01 00:00:00