Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli.

abstract：:In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fos...

journal_title：Applied and environmental microbiology

authors： Jogler C,Lin W,Meyerdierks A,Kube M,Katzmann E,Flies C,Pan Y,Amann R,Reinhardt R,Schüler D

更新日期：2009-06-01 00:00:00

abstract：:A model was constructed to simulate the results of experiments which investigated nitrification and denitrification in the freshwater sediment of Lake Vilhelmsborg, Denmark (K. Jensen, N. P. Sloth, N. Risgaard-Petersen, S. Rysgaard, and N. P. Revsbech, Appl. Environ. Microbiol. 60:2094-2100, 1994). The model output fa...

journal_title：Applied and environmental microbiology

authors： Blackburn TH,Blackburn ND,Jensen K,Risgaard-Petersen N

更新日期：1994-09-01 00:00:00

abstract：:Upon exposure of Nitrosomonas europaea to chloroform (7 microM, 1 h), transcripts for 175 of 2,460 genes were found at higher levels in treated cells than in untreated cells and transcripts for 501 genes were found at lower levels. With chloromethane (3.2 mM, 1 h), transcripts for 67 genes were at higher levels and tr...

journal_title：Applied and environmental microbiology

authors： Gvakharia BO,Permina EA,Gelfand MS,Bottomley PJ,Sayavedra-Soto LA,Arp DJ

更新日期：2007-05-01 00:00:00

abstract：:The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolat...

journal_title：Applied and environmental microbiology

authors： Tryfinopoulou P,Tsakalidou E,Nychas GJ

更新日期：2002-01-01 00:00:00

abstract：:A multilocus enzyme electrophoresis analysis of 96 strains of the salmonellae distinguished 80 electrophoretic types (ETs) and placed them in eight groups, seven of which correspond precisely to the seven taxonomic groups (I, II, IIIa, IIIb, IV, V, and VI) previously defined on the basis of biotype and genomic DNA hyb...

journal_title：Applied and environmental microbiology

authors： Boyd EF,Wang FS,Whittam TS,Selander RK

更新日期：1996-03-01 00:00:00

abstract：UNLABELLED:A structurally novel chitinase, Tc-ChiD, was identified from the hyperthermophilic archaeon Thermococcus chitonophagus, which can grow on chitin as the sole organic carbon source. The gene encoding Tc-ChiD contains regions corresponding to a signal sequence, two chitin-binding domains, and a putative catalyt...

journal_title：Applied and environmental microbiology

authors： Horiuchi A,Aslam M,Kanai T,Atomi H

更新日期：2016-05-31 00:00:00

abstract：:The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...

journal_title：Applied and environmental microbiology

authors： Kurtenbach K,Peacey M,Rijpkema SG,Hoodless AN,Nuttall PA,Randolph SE

更新日期：1998-04-01 00:00:00

abstract：UNLABELLED:Raw sausages are perishable foodstuffs; reducing their salt content raises questions about a possible increased spoilage of these products. In this study, we evaluated the influence of salt reduction (from 2.0% to 1.5% [wt/wt]), in combination with two types of packaging (modified atmosphere [50% mix of CO2-...

journal_title：Applied and environmental microbiology

authors： Fougy L,Desmonts MH,Coeuret G,Fassel C,Hamon E,Hézard B,Champomier-Vergès MC,Chaillou S

更新日期：2016-06-13 00:00:00

abstract：:Three predominant ruminal cellulolytic bacteria (Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1, and Ruminococcus albus 7) were grown in different binary combinations to determine the outcome of competition in either cellulose-excess batch culture or in cellulose-limited continuous culture. Relative popu...

journal_title：Applied and environmental microbiology

authors： Shi Y,Odt CL,Weimer PJ

更新日期：1997-02-01 00:00:00

abstract：:The world's largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of infection during the outbreak investigation, a laboratory method was needed to attempt detection of the parasite, Toxoplasma gond...

journal_title：Applied and environmental microbiology

authors： Isaac-Renton J,Bowie WR,King A,Irwin GS,Ong CS,Fung CP,Shokeir MO,Dubey JP

更新日期：1998-06-01 00:00:00

abstract：:Listeria monocytogenes strains are classified in at least three distinct phylogenetic lineages. There are correlations between lineage classification and source of bacterial isolation; e.g., human clinical and food isolates usually are classified in either lineage I or II. However, human clinical isolates are overrepr...

journal_title：Applied and environmental microbiology

authors： Oliver HF,Orsi RH,Wiedmann M,Boor KJ

更新日期：2010-07-01 00:00:00

abstract：:Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum str...

journal_title：Applied and environmental microbiology

authors： Bakan B,Giraud-Delville C,Pinson L,Richard-Molard D,Fournier E,Brygoo Y

更新日期：2002-11-01 00:00:00

abstract：:One of the first steps in characterizing an ecosystem is to describe the organisms inhabiting it. For microbial studies, experimental limitations have hindered the ability to depict diverse communities. Here we describe oligonucleotide fingerprinting of rRNA genes (OFRG), a method that permits identification of arraye...

journal_title：Applied and environmental microbiology

authors： Valinsky L,Della Vedova G,Scupham AJ,Alvey S,Figueroa A,Yin B,Hartin RJ,Chrobak M,Crowley DE,Jiang T,Borneman J

更新日期：2002-07-01 00:00:00

abstract：:Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

journal_title：Applied and environmental microbiology

authors： Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

更新日期：2014-11-01 00:00:00

abstract：:Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blen...

journal_title：Applied and environmental microbiology

authors： Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JA

更新日期：1990-06-01 00:00:00

abstract：:Broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. To gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an IncP-1 chloroaniline degradation plasmid, pWDL7::rfp and its close relativ...

journal_title：Applied and environmental microbiology

authors： Król JE,Penrod JT,McCaslin H,Rogers LM,Yano H,Stancik AD,Dejonghe W,Brown CJ,Parales RE,Wuertz S,Top EM

更新日期：2012-02-01 00:00:00

abstract：:The antifungal protein AFP from Aspergillus giganteus is highly effective in restricting the growth of major human- and plant-pathogenic filamentous fungi. However, a fundamental prerequisite for the use of AFP as an antifungal drug is a complete understanding of its mode of action. In this study, we performed several...

journal_title：Applied and environmental microbiology

authors： Hagen S,Marx F,Ram AF,Meyer V

更新日期：2007-04-01 00:00:00

abstract：:Pesticides are key stressors of soil microorganisms with reciprocal effects on ecosystem functioning. These effects have been mainly attributed to the parent compounds, while the impact of their transformation products (TPs) has been largely overlooked. We assessed in a meadow soil (soil A) the transformation of iprod...

journal_title：Applied and environmental microbiology

authors： Vasileiadis S,Puglisi E,Papadopoulou ES,Pertile G,Suciu N,Pappolla RA,Tourna M,Karas PA,Papadimitriou F,Kasiotakis A,Ipsilanti N,Ferrarini A,Sułowicz S,Fornasier F,Menkissoglu-Spiroudi U,Nicol GW,Trevisan M,Karpouzas DG

更新日期：2018-10-30 00:00:00

abstract：:The abundance of nifH, nirS, and nirK gene fragments involved in nitrogen (N) fixation and denitrification in thinned second-growth Douglas-fir (Pseudotsuga menziesii subsp. menziesii [Mirb.] Franco) forest soil was investigated by using quantitative real-time PCR. Prokaryotic N cycling is an important aspect of N ava...

journal_title：Applied and environmental microbiology

authors： Levy-Booth DJ,Winder RS

更新日期：2010-11-01 00:00:00

abstract：:A microbial community analysis using 16S rRNA gene sequencing was performed on borehole water and a granite rock core from Henderson Mine, a >1,000-meter-deep molybdenum mine near Empire, CO. Chemical analysis of borehole water at two separate depths (1,044 m and 1,004 m below the mine entrance) suggests that a sharp ...

journal_title：Applied and environmental microbiology

authors： Sahl JW,Schmidt R,Swanner ED,Mandernack KW,Templeton AS,Kieft TL,Smith RL,Sanford WE,Callaghan RL,Mitton JB,Spear JR

更新日期：2008-01-01 00:00:00

abstract：:Shewanella oneidensis couples oxidation of lactate to respiration of many substrates. Here we report that llpR (L-lactate-positive regulator, SO_3460) encodes a positive regulator of L-lactate utilization distinct from previously studied regulators. We also demonstrate D-lactate inhibition of L-lactate utilization in ...

journal_title：Applied and environmental microbiology

authors： Brutinel ED,Gralnick JA

更新日期：2012-12-01 00:00:00

abstract：:Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ...

journal_title：Applied and environmental microbiology

authors： Asgeirsdóttir SA,Scholtmeijer K,Wessels JG

更新日期：1999-05-01 00:00:00

abstract：:Leuconostoc mesenteroides strains that are resistant to high levels of nisin (up to 25,000 IU/ml in broth) were isolated. These nisin-resistant mutants were evaluated to determine their potential use as starter culture strains for cabbage fermentations. We found that some L. mesenteroides strains could be adapted to h...

journal_title：Applied and environmental microbiology

authors： Breidt F,Crowley KA,Fleming HP

更新日期：1993-11-01 00:00:00

abstract：:Two yellow pigments were isolated for the first time from the entomopathogenic bacterium Photorhabdus luminescens in liquid culture and were identified as the anthraquinone derivatives 3,8-dimethoxy-1-hydroxy-9,10-anthraquinone (minor) and 1,3-dimethoxy-8-hydroxy-9,10-anthraquinone (major). A known antibiotic, 3,5-dih...

journal_title：Applied and environmental microbiology

authors： Li J,Chen G,Wu H,Webster JM

更新日期：1995-12-01 00:00:00

abstract：:Fusarium isolates from specific diseased sorghum plants and rain-soaked wheat and wheat flour associated with human mycotoxicosis in India have been screened for their toxigenic potential. Of the 322 isolates screened, 11 isolates were found to produce deoxynivalenol in concentrations ranging from 0.01 to 186 microgra...

journal_title：Applied and environmental microbiology

authors： Ramakrishna Y,Bhat RV,Ravindranath V

更新日期：1989-10-01 00:00:00

abstract：:The efficiency of a system developed for the recovery of viruses contaminating large quantities of vegetables was investigated in the laboratory and tested in the field. Viruses seeded onto a number of leafy vegetables in the laboratory were eluted with a phosphate-buffered saline solution (pH 9.0). The eluate was cla...

journal_title：Applied and environmental microbiology

authors： Ward BK,Chenoweth CM,Irving LG

更新日期：1982-12-01 00:00:00

abstract：:Vibrio alginolyticus is one of the most abundant microorganisms in marine environments and is also an opportunistic pathogen mediating high-mortality vibriosis in marine animals. Alternative sigma factors play essential roles in bacterial pathogens in the adaptation to environmental changes during infection and the ad...

journal_title：Applied and environmental microbiology

authors： Gu D,Zhang J,Hao Y,Xu R,Zhang Y,Ma Y,Wang Q

更新日期：2019-07-01 00:00:00

abstract：:The pathogenic oomycete Aphanomyces invadans is the primary etiological agent in ulcerative mycosis, an ulcerative skin disease caused by a fungus-like agent of wild and cultured fish. We developed sensitive PCR and fluorescent peptide nucleic acid in situ hybridization (FISH) assays to detect A. invadans. Laboratory-...

journal_title：Applied and environmental microbiology

authors： Vandersea MW,Litaker RW,Yonnish B,Sosa E,Landsberg JH,Pullinger C,Moon-Butzin P,Green J,Morris JA,Kator H,Noga EJ,Tester PA

更新日期：2006-02-01 00:00:00

abstract：:O-antigen biosynthetic (wbf) regions for Vibrio cholerae serogroups O5, O8, and O108 were isolated and sequenced. Sequences were compared to those of other published V. cholerae O-antigen regions. These wbf regions showed a high degree of heterogeneity both in gene content and in gene order. Genes identified frequentl...

journal_title：Applied and environmental microbiology

authors： Aydanian A,Tang L,Morris JG,Johnson JA,Stine OC

更新日期：2011-04-01 00:00:00

abstract：:Adsorption of Agrobacterium tumefaciens cells to potato tuber disks reached equilibrium after coincubation for about 30 min. More than 10% of the number of bacteria bound at equilibrium were adsorbed within 30 s. Adsorption isotherms obtained at three temperatures showed that the heat of adsorption was nearly zero. ...

journal_title：Applied and environmental microbiology

authors： Kluepfel DA,Pueppke SG

更新日期：1986-05-01 00:00:00