Abstract:
:A model was constructed to simulate the results of experiments which investigated nitrification and denitrification in the freshwater sediment of Lake Vilhelmsborg, Denmark (K. Jensen, N. P. Sloth, N. Risgaard-Petersen, S. Rysgaard, and N. P. Revsbech, Appl. Environ. Microbiol. 60:2094-2100, 1994). The model output faithfully represented the profiles of O(2) and NO(3) and rates of nitrification, denitrification, and O(2) consumption as the O(2) concentration in the overlying water was increased from 10 to 600 muM. The model also accurately predicted the response, to increasing O(2) concentrations, of the integrated (micromoles per square meter per hour) rates of nitrification and denitrification. The simulated rates of denitrification of NO(3) diffusing from the overlying water (D(w)) and of NO(3) generated by nitrification within the sediment (D(n)) corresponded to the experimental rates as the O(2) concentration in the overlying water was altered. The predicted D(w) and D(n) rates, as NO(3) concentration in the overlying water was changed, closely resembled those determined experimentally. The model was composed of 41 layers 0.1 mm thick, of which 3 represented the diffusive boundary layer in the water. Large first-order rate constants for nitrification and denitrification were required to completely oxidize all NH(4) diffusing from the lower sediment layers and to remove much of the NO(3) produced. In addition to the flux of NH(4) from below, the model required a flux of an electron donor, possibly methane. Close coupling between nitrification and denitrification, achieved by allowing denitrification to tolerate some O(2) ( approximately 10 muM), was necessary to reproduce the real data. Spatial separation of the two processes (no toleration by denitrification of O(2)) resulted in too high NO(3) concentrations and too low rates of denitrification.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Blackburn TH,Blackburn ND,Jensen K,Risgaard-Petersen Ndoi
10.1128/AEM.60.9.3089-3095.1994keywords:
subject
Has Abstractpub_date
1994-09-01 00:00:00pages
3089-95issue
9eissn
0099-2240issn
1098-5336journal_volume
60pub_type
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