Abstract:
:A method was developed for the determination of dissolved DNA in aquatic environments. The method is based upon the concentration of dissolved DNA by ethanol precipitation of 0.2-mum-pore-size filtered water. The DNA in concentrated extracts was quantified by the fluorescence of Hoechst 33258-DNA complexes. Fluorescence not attributable to DNA was corrected for by DNase I digestion of the extracts and averaged 25% of the total fluorescence for all samples. The effectiveness of the procedure for concentrating dissolved DNA was demonstrated by the efficient (>90%) recovery of internal standards. Concentrations of dissolved DNA from a variety of marine and freshwater environments ranged from 0.2 to 44 mug/liter, with the highest values being obtained for estuarine and river environments. The method is simple, specific for DNA, and more sensitive than previously described methods for the determination of extracellular DNA.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Deflaun MF,Paul JH,Davis Ddoi
10.1128/AEM.52.4.654-659.1986keywords:
subject
Has Abstractpub_date
1986-10-01 00:00:00pages
654-9issue
4eissn
0099-2240issn
1098-5336journal_volume
52pub_type
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doi:10.1128/AEM.60.4.1335-1341.1994
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.32.5.723-725.1976
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pub_type: 杂志文章
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更新日期:1990-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.8.2653-2656.1993
更新日期:1993-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.6.1374-1378.1985
更新日期:1985-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00109-12
更新日期:2012-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.12.7388-7395.2004
更新日期:2004-12-01 00:00:00