Distinct acid resistance and survival fitness displayed by Curli variants of enterohemorrhagic Escherichia coli O157:H7.

abstract：:Sequence analysis of the five genes (gutRMCBA) downstream from the previously described sorbitol-6-phosphate dehydrogenase-encoding Lactobacillus casei gutF gene revealed that they constitute a sorbitol (glucitol) utilization operon. The gutRM genes encode putative regulators, while the gutCBA genes encode the EIIC, E...

journal_title：Applied and environmental microbiology

authors： Alcántara C,Sarmiento-Rubiano LA,Monedero V,Deutscher J,Pérez-Martínez G,Yebra MJ

更新日期：2008-09-01 00:00:00

abstract：:Flavobacterium psychrophilum, a member of the Cytophaga-Flavobacterium-Bacteroides group, is an important pathogen of salmonid fish. Previous attempts to develop genetic techniques for this fastidious, psychrotrophic bacterium have met with failure. Here we describe the development of techniques for the genetic manipu...

journal_title：Applied and environmental microbiology

authors： Alvarez B,Secades P,McBride MJ,Guijarro JA

更新日期：2004-01-01 00:00:00

abstract：:The oil-degrading Arthrobacter sp. RAG-1 produced an extracellular nondialyzable emulsifying agent when grown on hexadecane, ethanol, or acetate medium. The emulsifier was prepared by two procedures: (i) heptane extraction of the cell-free culture medium and (ii) precipitation with ammonium sulfate. A convenient assay...

journal_title：Applied and environmental microbiology

authors： Rosenberg E,Zuckerberg A,Rubinovitz C,Gutnick DL

更新日期：1979-03-01 00:00:00

abstract：:Four new primers and one published primer were used to PCR amplify hypervariable regions within the protozoal 18S rRNA gene to determine which primer pair provided the best identification and statistical analysis. PCR amplicons of 394 to 498 bases were generated from three primer sets, sequenced using Roche 454 pyrose...

journal_title：Applied and environmental microbiology

authors： Ishaq SL,Wright AD

更新日期：2014-09-01 00:00:00

abstract：:In the luminal contents of metronidazole-treated rats, there was a dominant Bifidobacterium species. A strain has been isolated, its 16S rRNA gene has been sequenced, and the strain has been named Bifidobacterium pseudolongum strain Patronus. In this study, using an experimental model of healthy rats, the effects of m...

journal_title：Applied and environmental microbiology

authors： Vasquez N,Suau A,Magne F,Pochart P,Pélissier MA

更新日期：2009-01-01 00:00:00

abstract：:7 alpha-Dehydroxylation of cholic acid and chenodeoxycholic acid by whole cells of strain c-25, a Eubacterium lentum-like intestinal anaerobe, was studied. 7 alpha-Dehydroxylase activity was observed only in whole cells grown in the presence of the primary bile acid (cholic acid or chenodeoxycholic acid). Chenodeoxych...

journal_title：Applied and environmental microbiology

authors： Masuda N,Oda H,Hirano S,Masuda M,Tanaka H

更新日期：1984-04-01 00:00:00

abstract：:The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

journal_title：Applied and environmental microbiology

authors： Fujito BT,Lytle CD

更新日期：1996-09-01 00:00:00

abstract：:The mechanisms of metal resistance of a cadmium-resistant Alcaligenes sp. were studied. Growth in a defined medium was unaffected by cadmium at concentrations up to 0.1 mM, while at concentrations up to 2.5 mM, growth occurred after an extended lag phase. The increase in length of the lag phase was abolished by repeat...

journal_title：Applied and environmental microbiology

authors： McEntee JD,Woodrow JR,Quirk AV

更新日期：1986-03-01 00:00:00

abstract：:By using thin-layer chromatography and infrared spectroscopy, xanthomegnin and viomellein have been isolated and identified from species of the Aspergillus ochraceus group. A correlation was established between the occurrence of these fungal quinones in the fungal cultural products and the ability of these products to...

journal_title：Applied and environmental microbiology

authors： Robbers JE,Hong S,Tuite J,Carlton WW

更新日期：1978-12-01 00:00:00

abstract：:We examined the effect of different sediment types on the staining effectiveness of the fluorochrome DAPI (4'-6-diamidino-2-phenylindole dihydrochloride) over a wide range of concentrations and on the masking effect of sediment particles on DAPI-stained bacteria. Sediment type greatly affects the staining efficiency o...

journal_title：Applied and environmental microbiology

authors： Schallenberg M,Kalff J,Rasmussen JB

更新日期：1989-05-01 00:00:00

abstract：:Pantoea vagans C9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of Erwinia amylovora, the causal agent of fire blight disease of pear and apple. One antibiotic, herbicolin I, was purified from culture filtrates of P. vagans C9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarb...

journal_title：Applied and environmental microbiology

authors： Kamber T,Lansdell TA,Stockwell VO,Ishimaru CA,Smits TH,Duffy B

更新日期：2012-06-01 00:00:00

abstract：:A seasonal study of the distribution of lysogenic bacteria in Tampa Bay, Florida, was conducted over a 13-month period. Biweekly water samples were collected and either were left unaltered or had the viral population reduced by filtration (pore size, 0.2 micro m) and resuspension in filtered (pore size, 0.2 micro m) w...

journal_title：Applied and environmental microbiology

authors： Williamson SJ,Houchin LA,McDaniel L,Paul JH

更新日期：2002-09-01 00:00:00

abstract：:The nucleotide sequence of an endo-beta-1,4-glucanase gene of Clostridium acetobutylicum contained two putative extended promoter consensus sequences, a Shine-Dalgarno sequence and a TTG initiation codon. The nucleotide sequence of the gene coding for the C-terminal region of this enzyme was not required for activity....

journal_title：Applied and environmental microbiology

authors： Zappe H,Jones WA,Jones DT,Woods DR

更新日期：1988-05-01 00:00:00

abstract：:Although Salmonella enterica can produce life-threatening colitis in horses, certain serotypes are more commonly associated with clinical disease. Our aim was to evaluate the proportional morbidity attributed to different serotypes, as well as the phenotypic and genotypic antimicrobial resistance (AMR) of Salmonella i...

journal_title：Applied and environmental microbiology

authors： Leon IM,Lawhon SD,Norman KN,Threadgill DS,Ohta N,Vinasco J,Scott HM

更新日期：2018-06-18 00:00:00

abstract：UNLABELLED:The efficiency of direct steam injection (DSI) at 105 °C for 3 s to inactivate Mycobacterium avium subsp. paratuberculosis in milk at a pilot-plant scale was investigated. Milk samples were artificially contaminated with M. avium subsp. paratuberculosis and also with cow fecal material naturally infected wit...

journal_title：Applied and environmental microbiology

authors： Peterz M,Butot S,Jagadeesan B,Bakker D,Donaghy J

更新日期：2016-04-18 00:00:00

abstract：:Mean pH values in pooled samples of foregut, midgut, and hindgut from adult Melanoplus sanguinipes, which had been raised in the laboratory on barley shoots and wheat bran, were 5.15, 6.39, and 5.98, respectively. Homogenates of midgut/hindgut sections and frass (feces) yielded colony counts of bacteria by the spread ...

journal_title：Applied and environmental microbiology

authors： Mead LJ,Khachatourians GG,Jones GA

更新日期：1988-05-01 00:00:00

abstract：:The recent advent of tools enabling statistical inferences to be drawn from comparisons of microbial communities has enabled the focus of microbial ecology to move from characterizing biodiversity to describing the distribution of that biodiversity. Although statistical tools have been developed to compare community s...

journal_title：Applied and environmental microbiology

authors： Schloss PD,Handelsman J

更新日期：2006-10-01 00:00:00

abstract：:When sucrose is provided as a substrate for glucosyltransferase (GTF), Spp+ cells of the oral bacteria Streptococcus gordonii grow embedded in an insoluble glucan mass associated with surfaces. Spp- phase variants with lower GTF activity, which either arise from or are grown with Spp+ cells, segregate preferentially a...

journal_title：Applied and environmental microbiology

authors： Vickerman MM,Clewell DB,Jones GW

更新日期：1991-12-01 00:00:00

abstract：:A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequenci...

journal_title：Applied and environmental microbiology

authors： Klyachko O,Stein BD,Grindle N,Clay K,Fuqua C

更新日期：2007-10-01 00:00:00

abstract：:The seasonal variations in community structure and cell morphology of pelagic procaryotes from a high mountain lake (Gossenkollesee, Austria) were studied by in situ hybridization with rRNA-targeted fluorescently labeled oligonucleotide probes (FISH) and image-analyzed microscopy. Compositional changes and biomass flu...

journal_title：Applied and environmental microbiology

authors： Pernthaler J,Glockner FO,Unterholzner S,Alfreider A,Psenner R,Amann R

更新日期：1998-11-01 00:00:00

abstract：:Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins w...

journal_title：Applied and environmental microbiology

authors： Martínez-Rodríguez S,Las Heras-Vázquez FJ,Mingorance-Cazorla L,Clemente-Jiménez JM,Rodríguez-Vico F

更新日期：2004-01-01 00:00:00

abstract：:Conjugal plasmid transfer was examined on the phylloplane of bean (Phaseolus vulgaris) and related to the spatial distribution pattern and metabolic activity of the bacteria. The donor (Pseudomonas putida KT2442) harbored a derivative of the TOL plasmid, which conferred kanamycin resistance and had the gfp gene insert...

journal_title：Applied and environmental microbiology

authors： Normander B,Christensen BB,Molin S,Kroer N

更新日期：1998-05-01 00:00:00

abstract：:Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bott...

journal_title：Applied and environmental microbiology

authors： Loy A,Beisker W,Meier H

更新日期：2005-07-01 00:00:00

abstract：:beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...

journal_title：Applied and environmental microbiology

authors： Araujo A,Ward OP

更新日期：1990-06-01 00:00:00

abstract：:Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Tilsala-Timisjarvi A,Rodtong S,Ng J,Munro K,Alatossava T

更新日期：1999-09-01 00:00:00

abstract：:Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, P...

journal_title：Applied and environmental microbiology

authors： Abeijón Mukdsi MC,Falentin H,Maillard MB,Chuat V,Medina RB,Parayre S,Thierry A

更新日期：2014-01-01 00:00:00

abstract：:Resources from the Sinorhizobium meliloti Rm1021 open reading frame (ORF) plasmid libraries were used in a medium-throughput method to construct a set of 50 overlapping deletion mutants covering all of the Rm1021 pSymA megaplasmid except the replicon region. Each resulting pSymA derivative carried a defined deletion o...

journal_title：Applied and environmental microbiology

authors： Yurgel SN,Mortimer MW,Rice JT,Humann JL,Kahn ML

更新日期：2013-03-01 00:00:00

abstract：:In Agrobacterium tumefaciens, the balance between acquiring enough iron and avoiding iron-induced toxicity is regulated in part by Fur (ferric uptake regulator). A fur mutant was constructed to address the physiological role of the regulator. Atypically, the mutant did not show alterations in the levels of siderophore...

journal_title：Applied and environmental microbiology

authors： Kitphati W,Ngok-Ngam P,Suwanmaneerat S,Sukchawalit R,Mongkolsuk S

更新日期：2007-08-01 00:00:00

abstract：:Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typi...

journal_title：Applied and environmental microbiology

authors： Varshney AK,Mediavilla JR,Robiou N,Guh A,Wang X,Gialanella P,Levi MH,Kreiswirth BN,Fries BC

更新日期：2009-11-01 00:00:00

abstract：:Biodegradability screening tests of soil commonly measure 14CO2 evolution from radiolabeled test compounds, and glucose has often served as a positive control. When constant amounts of radiolabel were added to soil in combination with increasing amounts of unlabeled substrates, glucose and some related hexoses behaved...

journal_title：Applied and environmental microbiology

authors： Shen J,Bartha R

更新日期：1996-07-01 00:00:00