Emergence of novel Streptococcus iniae exopolysaccharide-producing strains following vaccination with nonproducing strains.

abstract：:Vibrio cholerae, an environmental organism, is a facultative human pathogen. Here, we report the virulence profiles, comprising 18 genetic markers, of 102 clinical and 692 environmental V. cholerae strains isolated in Bangladesh between March 2004 and January 2006, showing the variability of virulence determinants wit...

journal_title：Applied and environmental microbiology

authors： Hasan NA,Ceccarelli D,Grim CJ,Taviani E,Choi J,Sadique A,Alam M,Siddique AK,Sack RB,Huq A,Colwell RR

更新日期：2013-09-01 00:00:00

abstract：:Reverse transcription-PCR analysis of drinking water in the homes of 56 children suffering from rotaviral gastroenteritis has shown the presence of the rotavirus genome in four samples. These strains were different from human rotaviruses detected in the children's feces, as determined by sequencing of the VP7-amplifie...

journal_title：Applied and environmental microbiology

authors： Gratacap-Cavallier B,Genoulaz O,Brengel-Pesce K,Soule H,Innocenti-Francillard P,Bost M,Gofti L,Zmirou D,Seigneurin JM

更新日期：2000-06-01 00:00:00

abstract：:The pigmented strain Serratia marcescens ATCC 274 had a higher buffering capacity and a higher membrane H conductance than S. marcescens GP, a spontaneous nonpigmented mutant of ATCC 274. The data suggest that mutations which apparently affect only the synthesis of a secondary metabolite can modify buffering capacity ...

journal_title：Applied and environmental microbiology

authors： Rius N,Solé M,Francia A,Lorén JG

更新日期：1994-06-01 00:00:00

abstract：:In earlier studies, the pathway of toluene and m- and p-xylene degradation (TOL pathway) in Pseudomonas putida (pWW0) was found to be subject to catabolite repression when the strain was grown at the maximal rate on glucose or succinate in the presence of an inducer. This report describes catabolite repression of the ...

journal_title：Applied and environmental microbiology

authors： Duetz WA,Marqués S,Wind B,Ramos JL,van Andel JG

更新日期：1996-02-01 00:00:00

abstract：:The erythromycin resistance gene ermB has been found in a variety of gram-positive bacteria. This gene has also been found in Bacteroides species but only in six recently isolated strains; thus, the gene seems to have entered this genus only recently. One of the six Bacteroides ermB-containing isolates, WH207, could t...

journal_title：Applied and environmental microbiology

authors： Gupta A,Vlamakis H,Shoemaker N,Salyers AA

更新日期：2003-11-01 00:00:00

abstract：:Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the lactase LacA (LacZ) from the thermophilic bacterium Thermoanaerobacter ethanolicus and the cellulase CelD, a cellulose-binding module (CBM) from Anaerocellum the...

journal_title：Applied and environmental microbiology

authors： Velikodvorskaya GA,Tikhonova TV,Gurvits ID,Karyagina AS,Lavrova NV,Sergienko OV,Tashlitskii VN,Lunina NA,Lunin VG

更新日期：2010-12-01 00:00:00

abstract：:Industrial production of the edible basidiomycete Pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. Optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields an...

journal_title：Applied and environmental microbiology

authors： Larraya LM,Alfonso M,Pisabarro AG,Ramírez L

更新日期：2003-06-01 00:00:00

abstract：:The single glucosyltransferase (GTF) of Streptococcus gordonii Challis CH1 makes alpha 1,3- and alpha 1,6-linked glucans from sucrose. The GTF carboxyl-terminal region has six direct repeats thought to be involved in glucan binding. Strains with defined mutations in this region have been described recently (M. M. Vick...

journal_title：Applied and environmental microbiology

authors： Vickerman MM,Clewell DB

更新日期：1997-05-01 00:00:00

abstract：:A gram-negative bacterium isolated from activated sludge was able to utilize up to 25 mM phosphonoacetate as the sole carbon and phosphorus source, with simultaneous excretion of virtually equimolar levels of phosphate. 2-Aminoethylphosphonate was similarly utilized with equivalent growth rates and cellular yields, wh...

journal_title：Applied and environmental microbiology

authors： McMullan G,Harrington F,Quinn JP

更新日期：1992-04-01 00:00:00

abstract：:Yeast extract agar, pork infusion agar, and modifications of these media were used to recover heated Clostridium botulinum spores. The D- and z-values were determined. Two type A strains and one type B strain of C. botulinum were studied. In all cases the D-values were largest when the spores were recovered in yeast e...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Pflug IJ

更新日期：1977-10-01 00:00:00

abstract：:Thiosulfate-oxidizing sox gene homologues were found at four loci (I, II, III, and IV) on the genome of Bradyrhizobium japonicum USDA110, a symbiotic nitrogen-fixing bacterium in soil. In fact, B. japonicum USDA110 can oxidize thiosulfate and grow under a chemolithotrophic condition. The deletion mutation of the soxY(...

journal_title：Applied and environmental microbiology

authors： Masuda S,Eda S,Ikeda S,Mitsui H,Minamisawa K

更新日期：2010-04-01 00:00:00

abstract：:A device for sampling at depths of up to 6,000 m is described in which 3 liters of seawater is concentrated over a Nucleopore filter to about 13 ml and retrieved under in situ pressure and temperature. Subsamples can be withdrawn into transfer units that are equipped with individual gas accumulators for preventing los...

journal_title：Applied and environmental microbiology

authors： Jannasch HW,Wirsen CO

更新日期：1977-03-01 00:00:00

abstract：:The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -inde...

journal_title：Applied and environmental microbiology

authors： Chouaia B,Rossi P,Montagna M,Ricci I,Crotti E,Damiani C,Epis S,Faye I,Sagnon N,Alma A,Favia G,Daffonchio D,Bandi C

更新日期：2010-11-01 00:00:00

abstract：:Previous studies have shown that the cellobiohydrolases of the white rot basidiomycete Phanerochaete chrysosporium are encoded by a family of structurally related genes. In this investigation, we identified and sequenced the most highly transcribed gene, cbh1-4. Evidence suggests that in this fungus the dominant isozy...

journal_title：Applied and environmental microbiology

authors： Vanden Wymelenberg A,Covert S,Cullen D

更新日期：1993-10-01 00:00:00

abstract：:A newly isolated rod-shaped, gram-negative anaerobic bacterium from human feces, named Julong 732, was found to be capable of metabolizing the isoflavone dihydrodaidzein to S-equol under anaerobic conditions. The metabolite, equol, was identified by using electron impact ionization mass spectrometry, (1)H and (13)C nu...

journal_title：Applied and environmental microbiology

authors： Wang XL,Hur HG,Lee JH,Kim KT,Kim SI

更新日期：2005-01-01 00:00:00

abstract：:The effect of addition of purified nisin Z in liposomes to cheese milk and of in situ production of nisin Z by Lactococcus lactis subsp. lactis biovar diacetylactis UL719 in the mixed starter on the inhibition of Listeria innocua in cheddar cheese was evaluated during 6 months of ripening. A cheese mixed starter cultu...

journal_title：Applied and environmental microbiology

authors： Benech RO,Kheadr EE,Laridi R,Lacroix C,Fliss I

更新日期：2002-08-01 00:00:00

abstract：:Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 microns in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two co...

journal_title：Applied and environmental microbiology

authors： Berk SG,Ting RS,Turner GW,Ashburn RJ

更新日期：1998-01-01 00:00:00

abstract：:A heat-stable extracellular protease from Pseudomonas fluorescens was purified by chromatography on a DEAE-cellulose column and gel filtration on a Sephadex G100 column. The homogeneous enzyme preparation was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relatedness of prot...

journal_title：Applied and environmental microbiology

authors： Jackman DM,Bartlett FM,Patel TR

更新日期：1983-07-01 00:00:00

abstract：:In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes. ...

journal_title：Applied and environmental microbiology

authors： Cao M,Bitar AP,Marquis H

更新日期：2007-04-01 00:00:00

abstract：:Most yeast species can ferment sugars to ethanol, but only a few can grow in the complete absence of oxygen. Oxygen availability might, therefore, be a key parameter in spoilage of food caused by fermentative yeasts. In this study, the oxygen requirement and regulation of alcoholic fermentation were studied in batch c...

journal_title：Applied and environmental microbiology

authors： Rodrigues F,Côrte-Real M,Leão C,van Dijken JP,Pronk JT

更新日期：2001-05-01 00:00:00

abstract：:From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with...

journal_title：Applied and environmental microbiology

authors： Jock S,Kim WS,Barny MA,Geider K

更新日期：2003-01-01 00:00:00

abstract：:Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to aut...

journal_title：Applied and environmental microbiology

authors： Ng TK,Zeikus JG

更新日期：1986-10-01 00:00:00

abstract：:This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active beta-galactosidase in the digestive tract, although it did not multiply during its transit. This...

journal_title：Applied and environmental microbiology

authors： Drouault S,Anba J,Corthier G

更新日期：2002-02-01 00:00:00

abstract：:Polyethylene glycol 6000 precipitation was found to be an effective concentration method that enhanced the chances for detecting human virus pathogens in environmental samples. Percent recoveries from eluates of fresh and estuarine waters with 8% polyethylene glycol 6000 averaged 86 for hepatitis A virus, 77 for human...

journal_title：Applied and environmental microbiology

authors： Lewis GD,Metcalf TG

更新日期：1988-08-01 00:00:00

abstract：:Fresh produce is known to carry nonpathogenic epiphytic microorganisms. During agricultural production and harvesting, leafy greens can become contaminated with antibiotic-resistant pathogens or commensals from animal and human sources. As lettuce does not undergo any inactivation or preservation treatment during proc...

journal_title：Applied and environmental microbiology

authors： Holvoet K,Sampers I,Callens B,Dewulf J,Uyttendaele M

更新日期：2013-11-01 00:00:00

abstract：:The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange. ...

journal_title：Applied and environmental microbiology

authors： Johansson HE,Johansson MK,Wong AC,Armstrong ES,Peterson EJ,Grant RE,Roy MA,Reddington MV,Cook RM

更新日期：2011-06-01 00:00:00

abstract：:Two-dimensional polyacrylamide electrophoresis was used to determine that mutants of Rhizobium trifolii DT6, claimed to be capable of effectively nodulating soybeans, were actually Rhizobium japonicum 110 contaminants isolated from the parent DT6 culture. ...

journal_title：Applied and environmental microbiology

authors： Leps WT,Roberts GP,Brill WJ

更新日期：1980-02-01 00:00:00

abstract：:Brevibacterium linens M18, isolated from red smear cheese, produces a substance that inhibits the growth of Listeria spp. and several coryneform and other gram-positive bacteria. No gram-negative bacteria were inhibited. The substance is heat labile, sensitive to proteolytic enzymes, and stable between pH 3 and 12. Hi...

journal_title：Applied and environmental microbiology

authors： Valdés-Stauber N,Scherer S

更新日期：1994-10-01 00:00:00

abstract：:This study quantified the levels of airborne microorganisms in six swine farms with more than 10,000 pigs in subtropical Taiwan. We evaluated breeding, growing, and finishing stalls, which were primarily open-air buildings, as well as partially enclosed farrowing and nursery piggeries. Airborne culturable bacteria, gr...

journal_title：Applied and environmental microbiology

authors： Chang CW,Chung H,Huang CF,Su HJ

更新日期：2001-01-01 00:00:00

abstract：:A phosphocholine-substituted beta-1,3;1,6 cyclic glucan (PCCG), an unusual cyclic oligosaccharide, has been isolated from Bradyrhizobium japonicum USDA 110 (D. B. Rolin, P. E. Pfeffer, S. F. Osman, B. S. Swergold, F. Kappler, and A. J. Benesi, Biochim. Biophys. Acta 1116:215-225, 1992). Data presented here suggest tha...

journal_title：Applied and environmental microbiology

authors： Pfeffer PE,Bécard G,Rolin DB,Uknalis J,Cooke P,Tu S

更新日期：1994-06-01 00:00:00