Elucidation of Growth Inhibition and Acetic Acid Production by Clostridium thermoaceticum.

Abstract:

:The production of acetic acid by Clostridium thermoaceticum was studied by using batch fermentations. In a pH-controlled fermentation with sodium hydroxide (pH 6.9), this organism was able to produce 56 g of acetic acid per liter. On the other hand, when the pH was not controlled and was decreased during fermentation to 5.4, the maximum attainable acetic acid concentration was only 15.3 g/liter. To obtain a better understanding of the end product inhibition, various salts were tested to determine their effect on the growth rate of C. thermoaceticum. An inverse linear relationship between the growth rate and the final cell concentration to the sodium acetate concentration was found. By using different concentrations of externally added sodium salts, the relative growth inhibition caused by the anion was found to be in the order of acetate > chloride > sulfate. Various externally added cations of acetate were also examined with respect to their inhibitory effects on growth. The relative magnitude of inhibition on the growth rate was found to be ammonium > potassium > sodium. The combined results have shown that the undissociated acetic acid was much more inhibitory than the ionized acetate ion. Complete growth inhibition resulted when the undissociated acetic acid concentration was between 0.04 and 0.05 M and when the ionized acetate concentration was 0.8 M. Therefore, at low pH (below 6.0), undissociated acetic acid is responsible for growth inhibition, and at high pH (above 6.0), ionized acetate ion is responsible for growth inhibition.

journal_name

Appl Environ Microbiol

authors

Wang G,Wang DI

doi

10.1128/AEM.47.2.294-298.1984

keywords:

subject

Has Abstract

pub_date

1984-02-01 00:00:00

pages

294-8

issue

2

eissn

0099-2240

issn

1098-5336

journal_volume

47

pub_type

杂志文章
  • Use of 16S-23S rRNA intergenic spacer region PCR and repetitive extragenic palindromic PCR analyses of Escherichia coli isolates to identify nonpoint fecal sources.

    abstract::Despite efforts to minimize fecal input into waterways, this kind of pollution continues to be a problem due to an inability to reliably identify nonpoint sources. Our objective was to find candidate source-specific Escherichia coli fingerprints as potential genotypic markers for raw sewage, horses, dogs, gulls, and c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.8.4942-4950.2003

    authors: Seurinck S,Verstraete W,Siciliano SD

    更新日期:2003-08-01 00:00:00

  • Chloroethene biodegradation in sediments at 4 degrees C.

    abstract::Microbial reductive dechlorination of [1,2-14C]trichloroethene to [14C]cis-dichloroethene and [14C]vinyl chloride was observed at 4 degrees C in anoxic microcosms prepared with cold temperature-adapted aquifer and river sediments from Alaska. Microbial anaerobic oxidation of [1,2-14C]cis-dichloroethene and [1,2-14C]vi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.10.6414-6417.2005

    authors: Bradley PM,Richmond S,Chapelle FH

    更新日期:2005-10-01 00:00:00

  • Detection of airborne Stachybotrys chartarum macrocyclic trichothecene mycotoxins in the indoor environment.

    abstract::The existence of airborne mycotoxins in mold-contaminated buildings has long been hypothesized to be a potential occupant health risk. However, little work has been done to demonstrate the presence of these compounds in such environments. The presence of airborne macrocyclic trichothecene mycotoxins in indoor environm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.11.7376-7388.2005

    authors: Brasel TL,Martin JM,Carriker CG,Wilson SC,Straus DC

    更新日期:2005-11-01 00:00:00

  • Oxygen requirements of the food spoilage yeast Zygosaccharomyces bailii in synthetic and complex media.

    abstract::Most yeast species can ferment sugars to ethanol, but only a few can grow in the complete absence of oxygen. Oxygen availability might, therefore, be a key parameter in spoilage of food caused by fermentative yeasts. In this study, the oxygen requirement and regulation of alcoholic fermentation were studied in batch c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.5.2123-2128.2001

    authors: Rodrigues F,Côrte-Real M,Leão C,van Dijken JP,Pronk JT

    更新日期:2001-05-01 00:00:00

  • Engineering trehalose synthesis in Lactococcus lactis for improved stress tolerance.

    abstract::Trehalose accumulation is a common cell defense strategy against a variety of stressful conditions. In particular, our team detected high levels of trehalose in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize tr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02922-10

    authors: Carvalho AL,Cardoso FS,Bohn A,Neves AR,Santos H

    更新日期:2011-06-01 00:00:00

  • Plate diffusion assay as a rapid method for dosimetry of mutagens.

    abstract::This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.6.1127-1131.1979

    authors: Awerbuch TE,Stark AA

    更新日期:1979-12-01 00:00:00

  • Effects of carbon substrates on nitrite accumulation in freshwater sediments

    abstract::The contribution of the biochemical pathways nitrification, denitrification, and dissimilatory NO3- reduction to NH4+ (DNRA) to the accumulation of NO2- in freshwaters is governed by the species compositions of the bacterial populations resident in the sediments, available carbon (C) and nitrogen (N) substrates, and e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.1.61-66.1999

    authors: Kelso BHL,Smith RV,Laughlin RJ

    更新日期:1999-01-01 00:00:00

  • Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale.

    abstract::Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00335-09

    authors: Lauber CL,Hamady M,Knight R,Fierer N

    更新日期:2009-08-01 00:00:00

  • The locus coding for the 3-nitrobenzoate dioxygenase of Comamonas sp. strain JS46 is flanked by IS1071 elements and is subject to deletion and inversion events.

    abstract::In Comamonas sp. strain JS46, 3-nitrobenzoate (3Nba) is initially oxidized at the 3,4 position by a dioxygenase, which results in release of nitrite and production of protocatechuate. The locus coding for the 3Nba dioxygenase (designated mnb, for m-nitrobenzoate) was mobilized from strain JS46 using a plasmid capture ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.4.2651-2660.2006

    authors: Providenti MA,Shaye RE,Lynes KD,McKenna NT,O'brien JM,Rosolen S,Wyndham RC,Lambert IB

    更新日期:2006-04-01 00:00:00

  • Comparative Profiling and Discovery of Novel Glycosylated Mycosporine-Like Amino Acids in Two Strains of the Cyanobacterium Scytonema cf. crispum.

    abstract:UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01633-16

    authors: D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

    更新日期:2016-09-16 00:00:00

  • Antagonism of Lactic Acid Bacteria against Phytopathogenic Bacteria.

    abstract::A variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens Xanthomonas campestris, Erwinia carotovora, and Pseudomonas syringae. Effective "in vitro" inhibition was found both on agar plates and in broth cultures. I...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.3.552-555.1986

    authors: Visser R,Holzapfel WH,Bezuidenhout JJ,Kotzé JM

    更新日期:1986-09-01 00:00:00

  • Bacteria on closed-boll and commercially harvested cotton.

    abstract::The bacterial content of specially treated cottons used by other investigators to test human pulmonary responses to cotton dust was examined. Cotton from Lubbock, Tex. and Stoneville, Miss. were either (i) harvested by machine and handled as commercial bale cotton, (ii) harvested as closed bolls with bracts intact and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.2.355-362.1982

    authors: Millner PD,Ericson KE,Marsh PB

    更新日期:1982-08-01 00:00:00

  • Comparison of the seasonal variations of Synechococcus assemblage structures in estuarine waters and coastal waters of Hong Kong.

    abstract::Seasonal variation in the phylogenetic composition of Synechococcus assemblages in estuarine and coastal waters of Hong Kong was examined through pyrosequencing of the rpoC1 gene. Sixteen samples were collected in 2009 from two stations representing estuarine and ocean-influenced coastal waters, respectively. Synechoc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01895-15

    authors: Xia X,Vidyarathna NK,Palenik B,Lee P,Liu H

    更新日期:2015-11-01 00:00:00

  • Isolation and characterization of a freeze-tolerant diploid derivative of an industrial baker's yeast strain and its use in frozen doughs.

    abstract::The routine production and storage of frozen doughs are still problematic. Although commercial baker's yeast is highly resistant to environmental stress conditions, it rapidly loses stress resistance during dough preparation due to the initiation of fermentation. As a result, the yeast loses gassing power significantl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.10.4780-4787.2002

    authors: Teunissen A,Dumortier F,Gorwa MF,Bauer J,Tanghe A,Loïez A,Smet P,Van Dijck P,Thevelein JM

    更新日期:2002-10-01 00:00:00

  • Suitability of quantitative real-time PCR to estimate the biomass of fungal root endophytes.

    abstract::A nested single-copy locus-based quantitative PCR (qPCR) assay and a multicopy locus-based qPCR assay were developed to estimate endophytic biomass of fungal root symbionts belonging to the Phialocephala fortinii sensu lato-Acephala applanata species complex (PAC). Both assays were suitable for estimation of endophyti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00907-10

    authors: Tellenbach C,Grünig CR,Sieber TN

    更新日期:2010-09-01 00:00:00

  • Characterization of a Yellowstone hot spring microbial community by 5S rRNA sequences.

    abstract::The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.6.1379-1384.1985

    authors: Stahl DA,Lane DJ,Olsen GJ,Pace NR

    更新日期:1985-06-01 00:00:00

  • Evidence from Serpula lacrymans that 2,5-dimethoxyhydroquinone Is a lignocellulolytic agent of divergent brown rot basidiomycetes.

    abstract::Basidiomycetes that cause brown rot of wood are essential biomass recyclers in coniferous forest ecosystems and a major cause of failure in wooden structures. Recent work indicates that distinct lineages of brown rot fungi have arisen independently from ligninolytic white rot ancestors via loss of lignocellulolytic en...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03880-12

    authors: Korripally P,Timokhin VI,Houtman CJ,Mozuch MD,Hammel KE

    更新日期:2013-04-01 00:00:00

  • Effect of spatial differences in microbial activity, pH, and substrate levels on methanogenesis initiation in refuse.

    abstract::The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02349-10

    authors: Staley BF,de Los Reyes FL 3rd,Barlaz MA

    更新日期:2011-04-01 00:00:00

  • Quantitative detection of hepatitis a virus and enteroviruses near the United States-Mexico border and correlation with levels of fecal indicator bacteria.

    abstract::For decades, untreated sewage flowing northward from Tijuana, Mexico, via the Tijuana River has adversely affected the water quality of the recreational beaches of San Diego, California. We used quantitative reverse transcription-PCR to measure the levels of hepatitis A virus (HAV) and enteroviruses in coastal waters ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01024-06

    authors: Gersberg RM,Rose MA,Robles-Sikisaka R,Dhar AK

    更新日期:2006-12-01 00:00:00

  • Systems Analysis of NADH Dehydrogenase Mutants Reveals Flexibility and Limits of Pseudomonas taiwanensis VLB120's Metabolism.

    abstract::Obligate aerobic organisms rely on a functional electron transport chain for energy conservation and NADH oxidation. Because of this essential requirement, the genes of this pathway are likely constitutively and highly expressed to avoid a cofactor imbalance and energy shortage under fluctuating environmental conditio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03038-19

    authors: Nies SC,Dinger R,Chen Y,Wordofa GG,Kristensen M,Schneider K,Büchs J,Petzold CJ,Keasling JD,Blank LM,Ebert BE

    更新日期:2020-05-19 00:00:00

  • Evolutionary relationships of "Candidatus endobugula" bacterial symbionts and their Bugula bryozoan hosts.

    abstract::Ribosomal gene sequences were obtained from bryozoans in the genus Bugula and their bacterial symbionts; analyses of host and symbiont phylogenetic trees did not support a history of strict cospeciation. Symbiont-derived compounds known to defend host larvae from predation were only detected in two out of four symbiot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02798-07

    authors: Lim-Fong GE,Regali LA,Haygood MG

    更新日期:2008-06-01 00:00:00

  • Inhibitor Evaluation with Immobilized Nitrobacter agilis Cells.

    abstract::Nitrobacter agilis was entrapped in calcium alginate beads and used as a floating bed supplied with a continuous flow of nitrite medium. Complete nitrite oxidation was achieved within 30 h, and the system could be maintained for at least 210 h. The immobilized Nitrobacter system was subjected to sulfur oxyanions, acid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.6.1231-1235.1986

    authors: Tsai YL,Schlasner SM,Tuovinen OH

    更新日期:1986-12-01 00:00:00

  • Application of flow cytometry to detection and characterization of Legionella spp.

    abstract::Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.4.852-857.1985

    authors: Tyndall RL,Hand RE Jr,Mann RC,Evans C,Jernigan R

    更新日期:1985-04-01 00:00:00

  • Reduction of Norwalk virus, poliovirus 1, and bacteriophage MS2 by ozone disinfection of water.

    abstract::Norwalk virus and other human caliciviruses (noroviruses) are major agents of gastroenteritis, and water is a major route of their transmission. In an effort to control Norwalk virus in drinking water, Norwalk virus reduction by bench-scale ozone disinfection was determined using quantitative reverse transcription (RT...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.3975-3978.2003

    authors: Shin GA,Sobsey MD

    更新日期:2003-07-01 00:00:00

  • Toward cloning of the magnetotactic metagenome: identification of magnetosome island gene clusters in uncultivated magnetotactic bacteria from different aquatic sediments.

    abstract::In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fos...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02701-08

    authors: Jogler C,Lin W,Meyerdierks A,Kube M,Katzmann E,Flies C,Pan Y,Amann R,Reinhardt R,Schüler D

    更新日期:2009-06-01 00:00:00

  • Response of a soil bacterial community to grassland succession as monitored by 16S rRNA levels of the predominant ribotypes.

    abstract::The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.3998-4003.2000

    authors: Felske A,Wolterink A,Van Lis R,De Vos WM,Akkermans AD

    更新日期:2000-09-01 00:00:00

  • Chlorine injury and the enumeration of waterborne coliform bacteria.

    abstract::Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.3.633-641.1979

    authors: Camper AK,McFeters GA

    更新日期:1979-03-01 00:00:00

  • Biochemical and Molecular Characterization of Obesumbacterium proteus, a Common Contaminant of Brewing Yeasts.

    abstract::We have evaluated the effectiveness of API 20E, Biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (ERIC)-PCR to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant Obesumbacterium proteus. Of the five typing techniques, Biolog tes...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.5.1635-1640.1994

    authors: Prest AG,Hammond JR,Stewart GS

    更新日期:1994-05-01 00:00:00

  • Genomic potential of Marinobacter aquaeolei, a biogeochemical "opportunitroph".

    abstract::The genus of Marinobacter is one of the most ubiquitous in the global oceans and assumed to significantly impact various biogeochemical cycles. The genome structure and content of Marinobacter aquaeolei VT8 was analyzed and compared with those from other organisms with diverse adaptive strategies. Here, we report the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01866-10

    authors: Singer E,Webb EA,Nelson WC,Heidelberg JF,Ivanova N,Pati A,Edwards KJ

    更新日期:2011-04-01 00:00:00

  • An improved enzyme-linked immunosorbent assay for whole-cell determination of methanogens in samples from anaerobic reactors.

    abstract::An enzyme-linked immunosorbent assay was developed for the detection of whole cells of methanogens in samples from anaerobic continuously stirred tank digesters treating slurries of solid waste. The assay was found to allow for quantitative analysis of the most important groups of methanogens in samples from anaerobic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.5.2001-2006.1997

    authors: Sørensen AH,Ahring BK

    更新日期:1997-05-01 00:00:00