Integron prevalence and diversity in manured soil.

abstract：:A physiological, unbalanced model is presented that explicitly describes growth of the marine cyanobacterium Trichodesmium sp. at the expense of N(2) (diazotrophy). The model involves the dynamics of intracellular reserves of carbon and nitrogen and allows the uncoupling of the metabolism of these elements. The result...

journal_title：Applied and environmental microbiology

authors： Rabouille S,Staal M,Stal LJ,Soetaert K

更新日期：2006-05-01 00:00:00

abstract：:We determined the prevalences of Escherichia coli O157:H7 in feces, hide, and carcasses of meat goats at a U.S. processing plant. Prevalences were 11.1%, 2.7%, and 2.7%, respectively. Sixteen pulsed-field gel electrophoresis (PFGE) subtypes were identified among 49 E. coli O157:H7 isolates, some of which were present ...

journal_title：Applied and environmental microbiology

authors： Jacob ME,Foster DM,Rogers AT,Balcomb CC,Sanderson MW

更新日期：2013-07-01 00:00:00

abstract：:The human intestine harbors both lactate-producing and lactate-utilizing bacteria. Lactate is normally present at <3 mmol liter(-1) in stool samples from healthy adults, but concentrations up to 100 mmol liter(-1) have been reported in gut disorders such as ulcerative colitis. The effect of different initial pH values...

journal_title：Applied and environmental microbiology

authors： Belenguer A,Duncan SH,Holtrop G,Anderson SE,Lobley GE,Flint HJ

更新日期：2007-10-01 00:00:00

abstract：:A new method for the rapid and sensitive detection of Legionella pneumophila in hot water systems has been developed. The method is based on an IF assay combined with detection by solid-phase cytometry. This method allowed the enumeration of L. pneumophila serogroup 1 and L. pneumophila serogroups 2 to 6, 8 to 10, and...

journal_title：Applied and environmental microbiology

authors： Aurell H,Catala P,Farge P,Wallet F,Le Brun M,Helbig JH,Jarraud S,Lebaron P

更新日期：2004-03-01 00:00:00

abstract：:A straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (sMMO) activity on solid media. Such activity results in the development of a colored complex between 1-naphthol, which is formed when sMMO reacts with naphthalene, and o-dianisidine (tetrazotized). Methanotro...

journal_title：Applied and environmental microbiology

authors： Graham DW,Korich DG,LeBlanc RP,Sinclair NA,Arnold RG

更新日期：1992-07-01 00:00:00

abstract：:Accumulating evidence suggests that pesticides have played a role in the increased rate of honey bee colony loss. One of the most commonly used pesticides in the United States is the neonicotinoid imidacloprid. Although the primary mode of action of imidacloprid is on the insect nervous system, it has also been shown ...

journal_title：Applied and environmental microbiology

authors： Raymann K,Motta EVS,Girard C,Riddington IM,Dinser JA,Moran NA

更新日期：2018-06-18 00:00:00

abstract：:The ability of certain oral biofilm bacteria to moderate pH through arginine metabolism by the arginine deiminase system (ADS) is a deterrent to the development of dental caries. Here, we characterize a novel Streptococcus strain, designated strain A12, isolated from supragingival dental plaque of a caries-free indivi...

journal_title：Applied and environmental microbiology

authors： Huang X,Palmer SR,Ahn SJ,Richards VP,Williams ML,Nascimento MM,Burne RA

更新日期：2016-01-29 00:00:00

abstract：:Enumeration of lactic acid streptococci from dairy starter cultures on Elliker agar was improved by the addition of 0.4% (wt/vol) diammonium phosphate. Agar plate counts were up to 7.75 times greater due to improved buffering by the phosphate salt. ...

journal_title：Applied and environmental microbiology

authors： Barach JT

更新日期：1979-07-01 00:00:00

abstract：:The prokaryote community activity and structural characteristics within marine sediment sampled across a continental shelf area located off eastern Antarctica (66 degrees S, 143 degrees E; depth range, 709 to 964 m) were studied. Correlations were found between microbial biomass and aminopeptidase and chitinase rates,...

journal_title：Applied and environmental microbiology

authors： Bowman JP,McCammon SA,Gibson JA,Robertson L,Nichols PD

更新日期：2003-05-01 00:00:00

abstract：UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method...

journal_title：Applied and environmental microbiology

authors： Figueroa-Cuilan W,Daniel JJ,Howell M,Sulaiman A,Brown PJ

更新日期：2016-07-29 00:00:00

abstract：:Protoplasts of Claviceps purpurea were prepared by treatment of mycelium with a lytic mixture of snail gut enzyme and cellulase from Trichoderma viride. Such protoplasts could be efficiently lysed by Triton X-100 treatment at high osmotic pressure without Ca or Mg, allowing the release of intact vacuoles in high yield...

journal_title：Applied and environmental microbiology

authors： Keller U,Madry N,Kleinkauf H,Glund K

更新日期：1984-04-01 00:00:00

abstract：:Biotin-labeled DNA probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (Lactobacillus delbrueckii 21 and Lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. The forestomach sections were incubated ...

journal_title：Applied and environmental microbiology

authors： Tannock GW

更新日期：1989-02-01 00:00:00

abstract：:Three cutinase gene-like genes from the basidiomycete Coprinopsis cinerea (Coprinus cinereus) found with a similarity search were cloned and expressed in Trichoderma reesei under the control of an inducible cbh1 promoter. The selected transformants of all three polyesterase constructs showed activity with p-nitropheny...

journal_title：Applied and environmental microbiology

authors： Kontkanen H,Westerholm-Parvinen A,Saloheimo M,Bailey M,Rättö M,Mattila I,Mohsina M,Kalkkinen N,Nakari-Setälä T,Buchert J

更新日期：2009-04-01 00:00:00

abstract：:The presence of PCR inhibitors in water samples is well known and contributes to the fact that a practical PCR assay has not been developed for legionella surveillance. In this study, we devised a new seminested PCR assay for detection of Legionella spp. in water samples as a means of overriding the PCR inhibitors wit...

journal_title：Applied and environmental microbiology

authors： Miyamoto H,Yamamoto H,Arima K,Fujii J,Maruta K,Izu K,Shiomori T,Yoshida S

更新日期：1997-07-01 00:00:00

abstract：:We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill the mycelia of zygomycete molds. This migration was restricted to zygomycete molds and several basidiomycete species. No migration was seen on any molds of the phylum Ascomycota. S. marcesce...

journal_title：Applied and environmental microbiology

authors： Hover T,Maya T,Ron S,Sandovsky H,Shadkchan Y,Kijner N,Mitiagin Y,Fichtman B,Harel A,Shanks RM,Bruna RE,García-Véscovi E,Osherov N

更新日期：2016-04-18 00:00:00

abstract：:Vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related Vibrio mimicus. The two species share many genes coding for proteins, such as ctxAB, and show almost identical 16S DNA coding for rRNA (rDNA) sequences. Primers targeting conserved...

journal_title：Applied and environmental microbiology

authors： Chun J,Huq A,Colwell RR

更新日期：1999-05-01 00:00:00

abstract：:To determine the potential for bacteriophage-mediated gene transfer in the marine environment, we established transduction systems by using marine phage host isolates. Plasmid pQSR50, which contains transposon Tn5 and encodes kanamycin and streptomycin resistance, was used in plasmid transduction assays. Both marine b...

journal_title：Applied and environmental microbiology

authors： Jiang SC,Paul JH

更新日期：1998-08-01 00:00:00

abstract：:Dental caries is one of the most common diseases worldwide. Bacteria and fungi are both commensals in the oral cavity; however, most research regarding caries has focused on bacterial impacts. The oral fungal mycobiome associated with caries is not well characterized, and its role in disease is unclear. ITS1 amplicon ...

journal_title：Applied and environmental microbiology

authors： O'Connell LM,Santos R,Springer G,Burne RA,Nascimento MM,Richards VP

更新日期：2020-03-18 00:00:00

abstract：:Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, P...

journal_title：Applied and environmental microbiology

authors： Abeijón Mukdsi MC,Falentin H,Maillard MB,Chuat V,Medina RB,Parayre S,Thierry A

更新日期：2014-01-01 00:00:00

abstract：:A mesophilic acetogenic bacterium (MPOB) oxidized propionate to acetate and CO(2) in cocultures with the formate- and hydrogen-utilizing methanogens Methanospirillum hungatei and Methanobacterium formicicum. Propionate oxidation did not occur in cocultures with two Methanobrevibacter strains, which grew only with hydr...

journal_title：Applied and environmental microbiology

authors： Dong X,Plugge CM,Stams AJ

更新日期：1994-08-01 00:00:00

abstract：:During incubation of seawater in bottles, the decrease in dissolved oxygen is often nonlinear over time scales frequently used to measure respiration. Numbers of bacteria always increase, and rates of assimilation of dissolved leucine often increase exponentially. This suggests that sample handling disrupts the previo...

journal_title：Applied and environmental microbiology

authors： Pomeroy LR,Sheldon JE,Sheldon WM

更新日期：1994-01-01 00:00:00

abstract：:This is the first description, to our knowledge, of the distribution of human polyomavirus and simian virus 40 (SV40) in urban sewage. Using a nested-PCR procedure, we report the detection of human polyomaviruses JC virus (JCV) and BK virus (BKV) but not SV40 in a high percentage of urban sewage samples obtained from ...

journal_title：Applied and environmental microbiology

authors： Bofill-Mas S,Pina S,Girones R

更新日期：2000-01-01 00:00:00

abstract：:Phages lytic to Vibrio vulnificus were found in estuarine waters, sediments, plankton, crustacea, molluscan shellfish, and the intestines of finfish of the U.S. Gulf Coast, but no apparent relationship between densities of V. vulnificus and its phages was observed. Phage diversity and abundance in molluscan shellfish ...

journal_title：Applied and environmental microbiology

authors： DePaola A,McLeroy S,McManus G

更新日期：1997-06-01 00:00:00

abstract：:Forty-two Helicobacter isolates were isolated from swine feces in The Netherlands and Denmark. All 12 isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis ( approximately 99% similarity). Species-specific PCR indicated that all of the isolates were H. canadensis isolates. Although the ...

journal_title：Applied and environmental microbiology

authors： Inglis GD,McConville M,de Jong A

更新日期：2006-06-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:The use of DNA probes for Shiga-like toxin I (SLT-I) and SLT-II for detection of SLT-producing Escherichia coli (SLTEC) in foods and calf fecal samples was evaluated. Enrichment cultures were prepared from food or fecal samples. Colonies formed by plating of enrichment cultures were probed for SLTEC by colony hybridiz...

journal_title：Applied and environmental microbiology

authors： Samadpour M,Liston J,Ongerth JE,Tarr PI

更新日期：1990-05-01 00:00:00

abstract：:Scanning and transmission electron microscopy were used to examine the adherence and in situ morphology of the microbial community colonizing the anal and vulvar pores of the subfamily Cyathostominae (Nematoda: Strongylidae) from the colon of Burchell's zebra (Equus burchelli antiquorum). Two different morphological t...

journal_title：Applied and environmental microbiology

authors： Mackie RI,Krecek RC,Els HJ,van Niekerk JP,Kirschner LM,Baecker AA

更新日期：1989-05-01 00:00:00

abstract：:The currently used microbial decontamination method for spacecraft and components uses dry-heat microbial reduction at temperatures of >110°C for extended periods to prevent the contamination of extraplanetary destinations. This process is effective and reproducible, but it is also long and costly and precludes the us...

journal_title：Applied and environmental microbiology

authors： Pottage T,Macken S,Giri K,Walker JT,Bennett AM

更新日期：2012-06-01 00:00:00

abstract：:Lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are widely produced by Gram-positive bacteria, including many species of the Bacillus group. In the present study, one novel gene cluster coding lantibiotic cerecidins was unveiled in Bacillus cereus strain As 1.1846 ...

journal_title：Applied and environmental microbiology

authors： Wang J,Zhang L,Teng K,Sun S,Sun Z,Zhong J

更新日期：2014-04-01 00:00:00

abstract：:A nonsporulating strain of Streptomyces diastaticus producing alpha-L-arabinofuranosidase activity (EC 3.2-1.55) was isolated from soil. Two alpha-L-arabinosidases were purified by ion-exchange chromatography and chromatofocusing. The enzymes had molecular weights of 38,000 (C1) and 60,000 (C2) and pIs of 8.8 and 8.3,...

journal_title：Applied and environmental microbiology

authors： Tajana E,Fiechter A,Zimmermann W

更新日期：1992-05-01 00:00:00