Influence of the Endogenous Storage Lipid Poly-beta-Hydroxybutyrate on the Reducing Power Availability during Cometabolism of Trichloroethylene and Naphthalene by Resting Methanotrophic Mixed Cultures.

abstract：:Confocal scanning laser microscopy (CSLM) was used to demonstrate the attachment of Escherichia coli O157:H7 transformed with a plasmid encoding for green fluorescent protein (GFP) to the surface and within the internal structures of nonwaxed Red Delicious cv. apples. Apples at 2 or 25 degrees C were inoculated with a...

journal_title：Applied and environmental microbiology

authors： Burnett SL,Chen J,Beuchat LR

更新日期：2000-11-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is widely used to produce recombinant proteins, taking advantage of this species' high-density cell growth and strong ability to secrete proteins. Circular plasmids containing the P. pastoris-specific autonomously replicating sequence (PARS1) permit transformation of P. pastori...

journal_title：Applied and environmental microbiology

authors： Nakamura Y,Nishi T,Noguchi R,Ito Y,Watanabe T,Nishiyama T,Aikawa S,Hasunuma T,Ishii J,Okubo Y,Kondo A

更新日期：2018-07-17 00:00:00

abstract：:The hindguts of lower termites and Cryptocercus cockroaches are home to a distinct community of archaea, bacteria, and protists (primarily parabasalids and some oxymonads). Within a host species, the composition of these hindgut communities appears relatively stable, but the evolutionary and ecological factors structu...

journal_title：Applied and environmental microbiology

authors： Tai V,James ER,Nalepa CA,Scheffrahn RH,Perlman SJ,Keeling PJ

更新日期：2015-02-01 00:00:00

abstract：:To observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (Danio rerio), a series of plasmids was constructed for the red fluorescent protein (RFP) labeling of a variety of fish and human pathogens. The aim of this study was to create a collection o...

journal_title：Applied and environmental microbiology

authors： Singer JT,Phennicie RT,Sullivan MJ,Porter LA,Shaffer VJ,Kim CH

更新日期：2010-06-01 00:00:00

abstract：:Four pure cultures of denitrifying bacteria, which had previously been isolated on defined alkylbenzenes, were capable of anaerobic growth with crude oil as the only source of organic substrates. Chemical analyses after growth revealed that the known growth substrates toluene, ethylbenzene, and m-xylene were selective...

journal_title：Applied and environmental microbiology

authors： Rabus R,Widdel F

更新日期：1996-04-01 00:00:00

abstract：:In this work we investigated the antibacterial properties of differently shaped silver nanoparticles against the gram-negative bacterium Escherichia coli, both in liquid systems and on agar plates. Energy-filtering transmission electron microscopy images revealed considerable changes in the cell membranes upon treatme...

journal_title：Applied and environmental microbiology

authors： Pal S,Tak YK,Song JM

更新日期：2007-03-01 00:00:00

abstract：:Morphological changes of Vibrio parahaemolyticus from rods to spheres took place after a culture was subjected to starvation at a wide range of temperatures. Scanning electron micrographs revealed that starved spherical cells gradually developed a rippled cell surface with blebs and an extracellular filamentous substa...

journal_title：Applied and environmental microbiology

authors： Jiang X,Chai TJ

更新日期：1996-04-01 00:00:00

abstract：:The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular charact...

journal_title：Applied and environmental microbiology

authors： Ohtomo T,Yamada T,Yoshida K

更新日期：1988-10-01 00:00:00

abstract：:Cyanobacterium Plectonema boryanum IU 594 and cyanophage LPP-1 were used as indicator organisms in a bioassay of 16 pesticides. Experiments such as spot tests, disk assays, growth curves, and one-step growth experiments were used to examine the effects of pesticides on the host and virus. Also, experiments were done i...

journal_title：Applied and environmental microbiology

authors： Mallison SM 3rd,Cannon RE

更新日期：1984-05-01 00:00:00

abstract：:A biofilm-forming marine bacterium, D2, isolated from the surface of the tunicate Ciona intestinalis, was found to produce a novel, 190-kDa protein with antibacterial activity. The protein contained at least two subunits of 60 and 80 kDa, joined together by noncovalent bonds, and was shown to be released by D2 cells i...

journal_title：Applied and environmental microbiology

authors： James SG,Holmström C,Kjelleberg S

更新日期：1996-08-01 00:00:00

abstract：:Beef carcass sponge samples collected from July to August 1999 at four large processing plants in the United States were surveyed for the presence of non-O157 Shiga toxin-producing Escherichia coli (STEC). Twenty-eight (93%) of 30 single-source lots surveyed included at least one sample containing non-O157 STEC. Of 33...

journal_title：Applied and environmental microbiology

authors： Arthur TM,Barkocy-Gallagher GA,Rivera-Betancourt M,Koohmaraie M

更新日期：2002-10-01 00:00:00

abstract：:Cytochrome P450 BM-3, a self-sufficient P450 enzyme from Bacillus megaterium that catalyzes the subterminal hydroxylation of long-chain fatty acids, has been engineered into a catalyst for the oxidation of polycyclic aromatic hydrocarbons. The activities of a triplet mutant (A74G/F87V/L188Q) towards naphthalene, fluor...

journal_title：Applied and environmental microbiology

authors： Li QS,Ogawa J,Schmid RD,Shimizu S

更新日期：2001-12-01 00:00:00

abstract：:Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of th...

journal_title：Applied and environmental microbiology

authors： Fassel TA,Buchholz LA,Collins ML,Remsen CC

更新日期：1992-07-01 00:00:00

abstract：:Campylobacter jejuni is a foodborne pathogen that is recognized as the leading cause of human bacterial gastroenteritis. The widespread use of antibiotics in medicine and in animal husbandry has led to an increased incidence of antibiotic resistance in Campylobacter In addition to a role in multidrug resistance (MDR),...

journal_title：Applied and environmental microbiology

authors： Vieira A,Ramesh A,Seddon AM,Karlyshev AV

更新日期：2017-10-31 00:00:00

abstract：:The spatial arrangements and associative behavior of Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, and Streptococcus oralis strains in an in vitro model of supragingival plaque were determined. Using species-specific fluorescence-labeled antibodies in conjunction with con...

journal_title：Applied and environmental microbiology

authors： Guggenheim M,Shapiro S,Gmür R,Guggenheim B

更新日期：2001-03-01 00:00:00

abstract：:The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (wt/vol) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts...

journal_title：Applied and environmental microbiology

authors： Bajpai P,Margaritis A

更新日期：1982-12-01 00:00:00

abstract：:Genomic probes were used to investigate hepatitis A virus (HAV) and enterovirus RNAs in two types of shellfish from natural beds (Atlantic coast, France). After elution concentration, nucleic acid extracted by proteinase K and purified by phenol-chloroform and ethanol precipitation was assayed by dot blot hybridizatio...

journal_title：Applied and environmental microbiology

authors： Le Guyader F,Apaire-Marchais V,Brillet J,Billaudel S

更新日期：1993-11-01 00:00:00

abstract：:The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...

journal_title：Applied and environmental microbiology

authors： Rawlings TK,Ruiz GM,Colwell RR

更新日期：2007-12-01 00:00:00

abstract：:Despite the benefits to the global food supply and agricultural economies, pesticides are believed to pose a threat to the health of both humans and wildlife. Chlorpyrifos (CP), a commonly used organophosphate insecticide, has poor target specificity and causes acute neurotoxicity in a wide range of species via the su...

journal_title：Applied and environmental microbiology

authors： Daisley BA,Trinder M,McDowell TW,Collins SL,Sumarah MW,Reid G

更新日期：2018-04-16 00:00:00

abstract：:The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity...

journal_title：Applied and environmental microbiology

authors： Garneau MÈ,Schnetzer A,Countway PD,Jones AC,Seubert EL,Caron DA

更新日期：2011-11-01 00:00:00

abstract：:The susceptibility of Trichoplusia ni larvae to several Bacillus thuringiensis insecticidal crystal proteins (ICPs) was tested. Neonatal larvae proved to be susceptible to solubilized trypsin-treated CryIA(a), CryIA(b), and CryIA(c) (50% lethal concentrations [LC(50)s], 570, 480, and 320 ng/cm, respectively) but showe...

journal_title：Applied and environmental microbiology

authors： Estada U,Ferre J

更新日期：1994-10-01 00:00:00

abstract：:Protein disulfide isomerase (PDI) is important in assisting the folding and maturation of secretory proteins in eukaryotes. A gene, pdiA, encoding PDIA was previously isolated from Aspergillus niger, and we report its functional characterization here. Functional analysis of PDIA showed that it catalyzes the refolding ...

journal_title：Applied and environmental microbiology

authors： Ngiam C,Jeenes DJ,Punt PJ,Van Den Hondel CA,Archer DB

更新日期：2000-02-01 00:00:00

abstract：:Two bacteriolytic enzymes were produced when Hartmanella glebae was grown in the presence of both Enterobacter aerogenes and Alcaligenes faecalis. The identification of enzyme I as N-acetylmuramidase was reported earlier. Enzyme II was purified by gel filtration on a Bio-Gel A column. A recovery of 68.76% with 72.3-fo...

journal_title：Applied and environmental microbiology

authors： Hemelt DM,Mares B,Upadhyay JM

更新日期：1979-09-01 00:00:00

abstract：:In Comamonas sp. strain JS46, 3-nitrobenzoate (3Nba) is initially oxidized at the 3,4 position by a dioxygenase, which results in release of nitrite and production of protocatechuate. The locus coding for the 3Nba dioxygenase (designated mnb, for m-nitrobenzoate) was mobilized from strain JS46 using a plasmid capture ...

journal_title：Applied and environmental microbiology

authors： Providenti MA,Shaye RE,Lynes KD,McKenna NT,O'brien JM,Rosolen S,Wyndham RC,Lambert IB

更新日期：2006-04-01 00:00:00

abstract：:Rhodococcus erythropolis N1-36, a desulfurization strain, was grown in continuous culture at 10 different dilution rates with 50 (mu)M dibenzothiophene sulfone (DBTO(inf2)) as the growth-limiting nutrient. The steady-state biomass, concentrations of substrate (DBTO(inf2)) and product (monohydroxybiphenyl), saturation ...

journal_title：Applied and environmental microbiology

authors： Wang P,Humphrey AE,Krawiec S

更新日期：1996-08-01 00:00:00

abstract：:Demethylation inhibitor (DMI)-resistant strains of the plant pathogenic fungus Penicillium digitatum were shown to be simultaneously resistant to cycloheximide, 4-nitroquinoline-N-oxide (4NQO), and acriflavine. A PMR1 (Penicillium multidrug resistance) gene encoding an ATP-binding cassette (ABC) transporter (P-glycopr...

journal_title：Applied and environmental microbiology

authors： Nakaune R,Adachi K,Nawata O,Tomiyama M,Akutsu K,Hibi T

更新日期：1998-10-01 00:00:00

abstract：:The existence of airborne mycotoxins in mold-contaminated buildings has long been hypothesized to be a potential occupant health risk. However, little work has been done to demonstrate the presence of these compounds in such environments. The presence of airborne macrocyclic trichothecene mycotoxins in indoor environm...

journal_title：Applied and environmental microbiology

authors： Brasel TL,Martin JM,Carriker CG,Wilson SC,Straus DC

更新日期：2005-11-01 00:00:00

abstract：:This study demonstrates that elution of enteroviruses from a mixture of primary- and activated-sludge solids with beef extract at pH 9.2 +/- 0.2 may be less efficient than elution with beef extract at pH 7.2 +/- 0.2 and that elution of enteroviruses from extended-aeration-sludge solids with beef extract is at best no ...

journal_title：Applied and environmental microbiology

authors： Berg G,Sullivan G

更新日期：1988-07-01 00:00:00

abstract：:Light microscopy and a radioassay detected no significant difference in adherence of Kanagawa-positive and Kanagawa-negative strains of Vibrio parahaemolyticus to human epithelial cell lines. ...

journal_title：Applied and environmental microbiology

authors： Gingras SP,Howard LV

更新日期：1980-02-01 00:00:00

abstract：:Total extracted DNA from two heavily polychlorobiphenyl-contaminated soils was analyzed with respect to biphenyl dioxygenase sequences and activities. This was done by PCR amplification and cloning of a DNA segment encoding the active site of the enzyme. The translated sequences obtained fell into three similarity clu...

journal_title：Applied and environmental microbiology

authors： Standfuss-Gabisch C,Al-Halbouni D,Hofer B

更新日期：2012-04-01 00:00:00