Abstract：
:During homologous recombination, eukaryotic RecA homologue Rad51 assembles into a nucleoprotein filament on single-stranded DNA to catalyse homologous pairing and DNA-strand exchange with a homologous template. Rad51 nucleoprotein filaments are highly dynamic and regulated via the coordinated actions of various accessory proteins including Rad51 mediators. Here, we identify a new Rad51 mediator complex. The PCSS complex, comprising budding yeast Psy3, Csm2, Shu1 and Shu2 proteins, binds to recombination sites and is required for Rad51 assembly and function during meiosis. Within the hetero-tetramer, Psy3-Csm2 constitutes a core sub-complex with DNA-binding activity. In vitro, purified Psy3-Csm2 stabilizes the Rad51-single-stranded DNA complex independently of nucleotide cofactor. The mechanism of Rad51 stabilization is inferred by our high-resolution crystal structure, which reveals Psy3-Csm2 to be a structural mimic of the Rad51-dimer, a fundamental unit of the Rad51-filament. Together, these results reveal a novel molecular mechanism for this class of Rad51-mediators, which includes the human Rad51 paralogues.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Sasanuma H,Tawaramoto MS,Lao JP,Hosaka H,Sanda E,Suzuki M,Yamashita E,Hunter N,Shinohara M,Nakagawa A,Shinohara Adoi
10.1038/ncomms2678subject
Has Abstractpub_date
2013-01-01 00:00:00pages
1676issn
2041-1723pii
ncomms2678journal_volume
4pub_type
杂志文章abstract：:As a versatile intermediate, difluorocarbene is an electron-deficient transient species, meaning that its oxidation would be challenging. Herein we show that the oxidation of difluorocarbene could occur smoothly to generate carbonyl fluoride. The oxidation process is confirmed by successful trifluoromethoxylation, 18O...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-13359-z
更新日期：2019-11-25 00:00:00
abstract：:Inflammation is recognized as one of the drivers of cancer. Yet, the individual immune components that possess pro- and anti-tumorigenic functions in individual cancers remain largely unknown. NKG2D is a potent activating immunoreceptor that has emerged as an important player in inflammatory disorders besides its well...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms13930
更新日期：2017-01-27 00:00:00
abstract：:Herein, we report on a significant discovery, namely, the quantitative discharging of reduced graphite forms, such as graphite intercalation compounds, graphenide dispersions and graphenides deposited on surfaces with the simple solvent benzonitrile. Because of its comparatively low reduction potential, benzonitrile i...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms12411
更新日期：2016-08-10 00:00:00
abstract：:Biological tissues exhibit complex spatial heterogeneity that directs the functions of multicellular organisms. Quantifying protein expression is essential for elucidating processes within complex biological assemblies. Imaging mass spectrometry (IMS) is a powerful emerging tool for mapping the spatial distribution of...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-13858-z
更新日期：2020-01-07 00:00:00
abstract：:Amino-terminal acetylation is among the most ubiquitous of protein modifications in eukaryotes. Although loss of N-terminal acetylation is associated with many abnormalities, the molecular basis of these effects is known for only a few cases, where acetylation of single factors has been linked to binding avidity or me...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms5383
更新日期：2014-07-15 00:00:00
abstract：:After stroke, peripheral immune cells are activated and these systemic responses may amplify brain damage, but how the injured brain sends out signals to trigger systemic inflammation remains unclear. Here we show that a brain-to-cervical lymph node (CLN) pathway is involved. In rats subjected to focal cerebral ischem...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-13324-w
更新日期：2019-11-22 00:00:00
abstract：:Current machine learning classifiers have successfully been applied to whole-genome sequencing data to identify genetic determinants of antimicrobial resistance (AMR), but they lack causal interpretation. Here we present a metabolic model-based machine learning classifier, named Metabolic Allele Classifier (MAC), that...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-16310-9
更新日期：2020-05-22 00:00:00
abstract：:CD4(+) Th17 are heterogeneous in terms of cytokine production and capacity to initiate autoimmune diseases, such as experimental autoimmune encephalomyelitis (EAE). Here we demonstrate that experimental priming of encephalitogenic Th cells expressing RORγt and T-bet and producing IL-17A, IFN-γ and GM-CSF but not IL-10...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms11541
更新日期：2016-05-18 00:00:00
abstract：:dDsk2 is a conserved extraproteasomal ubiquitin receptor that targets ubiquitylated proteins for degradation. Here we report that dDsk2 plays a nonproteolytic function in transcription regulation. dDsk2 interacts with the dHP1c complex, localizes at promoters of developmental genes and is required for transcription. T...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms8049
更新日期：2015-04-28 00:00:00
abstract：:High-intensity lasers can be used to generate shockwaves, which have found applications in nuclear fusion, proton imaging, cancer therapies and materials science. Collisionless electrostatic shocks are one type of shockwave widely studied for applications involving ion acceleration. Here we show a novel mechanism for ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms9905
更新日期：2015-11-13 00:00:00
abstract：:Cells couple growth with division and regulate size in response to nutrient availability. In rod-shaped fission yeast, cell-size control occurs at mitotic commitment. An important regulator is the DYRK-family kinase Pom1, which forms gradients from cell poles and inhibits the mitotic activator Cdr2, itself localized a...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms9445
更新日期：2015-10-07 00:00:00
abstract：:Centromeres consist of specialized centrochromatin containing CENP-A nucleosomes intermingled with H3 nucleosomes carrying transcription-associated modifications. We have designed a novel synthetic biology 'in situ epistasis' analysis in which H3 dimethylated on lysine 4 (H3K4me2) demethylase LSD2 plus synthetic modul...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms13334
更新日期：2016-11-14 00:00:00
abstract：:Oocyte-specific miRNA function remains unclear in mice and worms because loss of Dgcr8 and Dicer from mouse and worm oocytes, respectively, does not yield oogenic defects. These data lead to several models: (a) miRNAs are not generated in oocytes; (b) miRNAs are generated but do not perform an oogenic function; (c) fu...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-07791-w
更新日期：2018-12-14 00:00:00
abstract：:Excessive nitrogen runoff leads to degraded water quality, harming human and ecosystem health. We examine the impact of changes in land use and land management for six combinations of socioeconomic pathways and climate outcomes, and find that societal choices will substantially impact riverine total nitrogen loading (...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-08884-w
更新日期：2019-02-26 00:00:00
abstract：:In certain regions of the predominantly nitrogen limited ocean, microbes can become co-limited by phosphorus. Within such regions, a proportion of the dissolved organic phosphorus pool can be accessed by microbes employing a variety of alkaline phosphatase (APase) enzymes. In contrast to the PhoA family of APases that...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms15465
更新日期：2017-05-19 00:00:00
abstract：:Fluctuations in glacier motion are very common and are thought to be controlled by subglacial hydrology and till deformation. There are few instrumented studies that have monitored seasonal changes. We use the innovative Glacsweb subglacial in situ wireless probes, combined with dGPS and custom geophone data from an I...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-09547-6
更新日期：2019-04-08 00:00:00
abstract：:Brainstem regions support vital bodily functions, yet their genetic architectures and involvement in common brain disorders remain understudied. Here, using imaging-genetics data from a discovery sample of 27,034 individuals, we identify 45 brainstem-associated genetic loci, including the first linked to midbrain, pon...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-17376-1
更新日期：2020-08-11 00:00:00
abstract：:Ultrasound sensors have wide applications across science and technology. However, improved sensitivity is required for both miniaturisation and increased spatial resolution. Here, we introduce cavity optomechanical ultrasound sensing, where dual optical and mechanical resonances enhance the ultrasound signal. We achie...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-08038-4
更新日期：2019-01-10 00:00:00
abstract：:In Arabidopsis thaliana, CONSTANS (CO) plays an essential role in the regulation of photoperiodic flowering under long-day conditions. CO protein is stable only in the afternoon of long days, when it induces the expression of FLOWERING LOCUS T (FT), which promotes flowering. The blue-light photoreceptor FLAVIN-BINDING...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-02476-2
更新日期：2017-12-22 00:00:00
abstract：:Cytokines are involved in early host defense against pathogen infections. In particular, tumor necrosis factor (TNF) and interferon-gamma (IFN-γ) have critical functions in non-cytopathic elimination of hepatitis B virus (HBV) in hepatocytes. However, the molecular mechanisms and mediator molecules are largely unknown...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-05782-5
更新日期：2018-08-16 00:00:00
abstract：:Heart failure (HF) is a leading cause of mortality. Inflammation is implicated in HF, yet clinical trials targeting pro-inflammatory cytokines in HF were unsuccessful, possibly due to redundant functions of individual cytokines. Searching for better cardiac inflammation targets, here we link T cells with HF developmen...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms14680
更新日期：2017-03-06 00:00:00
abstract：:Roughly a third (~30 ppm) of the carbon dioxide (CO2) that entered the ocean during ice ages is attributed to biological mechanisms. A leading hypothesis for the biological drawdown of CO2 is iron (Fe) fertilisation of the high latitudes, but modelling efforts attribute at most 10 ppm to this mechanism, leaving ~20 pp...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-12549-z
更新日期：2019-10-10 00:00:00
abstract：:RNA-binding proteins (RBPs) integrate the processing of RNAs into post-transcriptional gene regulation, but the direct contribution of them to myeloid cell specification is poorly understood. Here, we report the first global RBP transcriptomic analysis of myeloid differentiation by combining RNA-seq analysis with myel...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-01425-3
更新日期：2017-11-10 00:00:00
abstract：:Ion distribution in aqueous electrolytes near the interface plays a critical role in electrochemical, biological and colloidal systems, and is expected to be particularly significant inside nanoconfined regions. Electroneutrality of the total charge inside nanoconfined regions is commonly assumed a priori in solving i...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms7358
更新日期：2015-02-20 00:00:00
abstract：:Mucosal-associated invariant T (MAIT) cells are thought to detect microbial antigens presented by the HLA-Ib molecule MR1 through the exclusive use of a TRAV1-2-containing TCRα. Here we use MR1 tetramer staining and ex vivo analysis with mycobacteria-infected MR1-deficient cells to demonstrate the presence of function...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms12506
更新日期：2016-08-16 00:00:00
abstract：:MeCP2 plays a multifaceted role in gene expression regulation and chromatin organization. Interaction between MeCP2 and methylated DNA in the regulation of gene expression is well established. However, the widespread distribution of MeCP2 suggests it has additional interactions with chromatin. Here we demonstrate, by ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-16907-0
更新日期：2020-06-19 00:00:00
abstract：:Magnetized plasma interactions are ubiquitous in astrophysical and laboratory plasmas. Various physical effects have been shown to be important within colliding plasma flows influenced by opposing magnetic fields, however, experimental verification of the mechanisms within the interaction region has remained elusive. ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-20387-7
更新日期：2021-01-12 00:00:00
abstract：:Cytokinesis mediates the physical separation of dividing cells and, in 3D epithelia, provides a spatial landmark for lumen formation. Here, we unravel an unexpected role in cytokinesis for proteins of the intraflagellar transport (IFT) machinery, initially characterized for their ciliary role and their link to polycys...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-01479-3
更新日期：2017-12-04 00:00:00
abstract：:Field-effect transistors based on carbon nanotubes have been shown to be faster and less energy consuming than their silicon counterparts. However, ensuring these advantages are maintained for integrated circuits is a challenge. Here we demonstrate that a significant reduction in the use of field-effect transistors ca...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms1682
更新日期：2012-02-14 00:00:00
abstract：:Recent discovery of the half quantized thermal Hall conductivity in [Formula: see text]-RuCl[Formula: see text], a candidate material for the Kitaev spin liquid, suggests the presence of a highly entangled quantum state in external magnetic fields. This field induced phase appears between the low field zig-zag magneti...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-15320-x
更新日期：2020-04-02 00:00:00
